##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3390595 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.914152530750503 31.0 30.0 33.0 25.0 34.0 2 29.947078610096458 31.0 30.0 34.0 25.0 34.0 3 29.943057192026767 31.0 30.0 34.0 25.0 34.0 4 33.39205242737631 35.0 35.0 37.0 26.0 37.0 5 33.973935548185494 35.0 35.0 37.0 28.0 37.0 6 34.054181640685485 37.0 35.0 37.0 29.0 37.0 7 33.982246478862855 37.0 35.0 37.0 28.0 37.0 8 33.78242107948605 37.0 35.0 37.0 28.0 37.0 9 34.85363719347194 39.0 35.0 39.0 26.0 39.0 10 35.02402144756304 39.0 35.0 39.0 27.0 39.0 11 35.255993712018096 39.0 35.0 39.0 27.0 39.0 12 35.05669801318058 39.0 35.0 39.0 27.0 39.0 13 34.885351391127514 39.0 35.0 39.0 27.0 39.0 14 35.787940464726695 39.0 35.0 41.0 26.0 41.0 15 35.78519669851457 39.0 35.0 41.0 26.0 41.0 16 35.924583443318944 39.0 35.0 41.0 27.0 41.0 17 35.961359584379736 39.0 35.0 41.0 27.0 41.0 18 35.860165841098684 39.0 35.0 41.0 27.0 41.0 19 36.161474313505444 39.0 35.0 41.0 27.0 41.0 20 36.273053254664745 39.0 36.0 41.0 27.0 41.0 21 36.34175122655463 40.0 36.0 41.0 27.0 41.0 22 36.37283367668507 39.0 36.0 41.0 27.0 41.0 23 36.38826105742503 39.0 36.0 41.0 27.0 41.0 24 36.426342279157495 39.0 36.0 41.0 27.0 41.0 25 35.38934464304938 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.071589909206093 1101 2 1.0485741512307563 1101 3 1.3453325679153672 1101 4 1.1796248966416485 1101 5 0.8457895726856748 1101 6 0.8572164133196125 1101 7 0.8384725618674196 1101 8 1.0305847030681932 1101 9 1.2361686773500438 1101 10 1.147286337535526 1101 11 0.953116575487698 1101 12 1.1048293971223515 1101 13 1.195993605816895 1101 14 1.2745585418392409 1101 15 1.3975255943493465 1101 16 1.362208412860383 1101 17 1.2555257499220858 1101 18 1.370986900224466 1101 19 1.3060428850347066 1101 20 1.1112454984761868 1101 21 1.0045435200615742 1101 22 1.0261392380431147 1101 23 1.1129355979620499 1101 24 1.1471148354135252 1101 25 1.0762057685371076 1102 1 0.7777987256192311 1102 2 0.6474117210957289 1102 3 1.001626959424268 1102 4 0.7989469661568194 1102 5 0.6602333064958117 1102 6 0.515185501825556 1102 7 0.43627956961658754 1102 8 0.5950784517845662 1102 9 0.8337119978736567 1102 10 0.7397816975653626 1102 11 0.6092612822729464 1102 12 0.7613440811986223 1102 13 0.8170243949126288 1102 14 1.0428567915188722 1102 15 1.0278193612973325 1102 16 0.9440189976180449 1102 17 0.9463459126227605 1102 18 1.0110789271638154 1102 19 0.6856873978497404 1102 20 0.046353985731812486 1102 21 0.3301004964789698 1102 22 0.3662955881667429 1102 23 0.5772095788496046 1102 24 0.6047495068179671 1102 25 0.6872245460012252 1103 1 0.6783251591305302 1103 2 0.7243219022696188 1103 3 0.9477202249584877 1103 4 0.8190401921143007 1103 5 0.5917311500716096 1103 6 0.5283590399670572 1103 7 0.4382912822989553 1103 8 0.5803798303371792 1103 9 0.7325393168733356 1103 10 0.7216443027104233 1103 11 0.6397966224855693 1103 12 0.8835106734290932 1103 13 0.8773454396688365 1103 14 0.6861025204365063 1103 15 0.9625134860918863 1103 16 0.830464918013547 1103 17 0.8750888668869052 1103 18 0.915107998745313 1103 19 0.7996130052909791 1103 20 -0.07114139024888999 1103 21 0.34662966301076636 1103 22 -0.16006220480018385 1103 23 0.35477718382847456 1103 24 0.41452677058197906 1103 25 0.48842014086838503 1104 1 0.36567574969113537 1104 2 0.3837266504014849 1104 3 0.6070669856650781 1104 4 0.5263662852832098 1104 5 0.34922364101787196 1104 6 0.2651589901303595 1104 7 0.22587557419056026 1104 8 0.36264057994154086 1104 9 0.6353506263565336 1104 10 0.7279878162624556 1104 11 0.47958676027244707 1104 12 0.45775604937336567 1104 13 0.49640238190785624 1104 14 0.8000595357213172 1104 15 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0.4180707396459411 2207 9 -0.9423052709954192 2207 10 0.039966396542567395 2207 11 0.25732798355377895 2207 12 0.5109880717790318 2207 13 0.6808193645460605 2207 14 0.8792021245953592 2207 15 0.8661293205516571 2207 16 0.8398815920842964 2207 17 0.7842749181041242 2207 18 0.8461105966153326 2207 19 -0.20005312172590095 2207 20 0.33799573754366463 2207 21 -0.17636791862567236 2207 22 0.3078179165317181 2207 23 -0.3745495832759005 2207 24 -1.3510436179952663 2207 25 -0.113975614821058 2208 1 0.48850035264281644 2208 2 0.14463352450757583 2208 3 0.6053559161210949 2208 4 0.40062043086624755 2208 5 0.3679094112564627 2208 6 0.15711623849544054 2208 7 0.2576026738515793 2208 8 0.4392984590020177 2208 9 -0.20927979178052425 2208 10 -6.610252092471569E-4 2208 11 0.1080769873303069 2208 12 0.20671054035274494 2208 13 0.42832495424845973 2208 14 0.6274956453702387 2208 15 0.6298704662131343 2208 16 0.4256975462754653 2208 17 0.42085390107619247 2208 18 0.6354762266865421 2208 19 -1.3106718743377925 2208 20 -0.16816936965851426 2208 21 -0.21717808723891352 2208 22 0.07472358825403091 2208 23 0.2682073192143548 2208 24 0.42517073384443194 2208 25 0.492174507771189 2209 1 0.16189382048230527 2209 2 -1.3944751233122972 2209 3 -0.1568575987109213 2209 4 0.0790713824151581 2209 5 0.08400628107620634 2209 6 0.13672999376592543 2209 7 0.11100950768022955 2209 8 0.2352019202207387 2209 9 -2.1663630193308876 2209 10 -0.3401779952863535 2209 11 -0.12104861065459005 2209 12 0.24502226907045355 2209 13 0.3973523234135996 2209 14 0.5348465038301384 2209 15 0.7438434629326167 2209 16 0.5365172771635969 2209 17 0.33833820786173163 2209 18 0.5339515829884718 2209 19 0.35679357818930413 2209 20 0.2943742502595441 2209 21 0.3691209649456866 2209 22 0.2618074256120977 2209 23 0.2382559295995037 2209 24 0.3505022078865352 2209 25 0.37278710316253694 2210 1 0.20939632597480085 2210 2 0.1263640860946431 2210 3 0.1934262074831139 2210 4 0.3176771452111211 2210 5 0.10133110879299068 2210 6 0.0809276975269384 2210 7 0.09189488507105636 2210 8 0.24813001666669265 2210 9 0.40933141831254005 2210 10 -1.4759709959033458 2210 11 -0.4543111454621709 2210 12 -2.3056623751169667 2210 13 -0.6827079553081603 2210 14 -0.49871407848875293 2210 15 -0.6798341348631993 2210 16 -0.13945219336952874 2210 17 0.15432080162591433 2210 18 0.3785316395946481 2210 19 0.25271554229954774 2210 20 0.2589256936517401 2210 21 0.3085668430432804 2210 22 0.3331122322403033 2210 23 0.14710668385884418 2210 24 0.2566808584788802 2210 25 0.19682677318836284 2211 1 -0.15601015664192985 2211 2 0.021758971521691706 2211 3 -1.411728884089186 2211 4 -0.4882355264353677 2211 5 -0.3185529581405646 2211 6 -0.2507705827402944 2211 7 -0.2949711678313278 2211 8 0.03523715115632342 2211 9 0.19550480957231287 2211 10 0.2870828699341814 2211 11 -0.02860231541166769 2211 12 -0.029713562968943563 2211 13 -0.019673698795983796 2211 14 -0.5812979122587407 2211 15 -0.09958596758172433 2211 16 0.12478627240293605 2211 17 -0.0035991605026808315 2211 18 0.1418916109682442 2211 19 0.06139569921973731 2211 20 0.13130728842169503 2211 21 -4.81463554727668E-4 2211 22 -0.09166384644247216 2211 23 -0.30813834428450804 2211 24 -0.061059368102107214 2211 25 0.03172243710056222 2212 1 -0.43257999524141155 2212 2 -0.4174172706596764 2212 3 -2.5975599449062443 2212 4 -1.47885130097999 2212 5 -0.6731174596849883 2212 6 -0.5058316201639528 2212 7 -0.408470883703032 2212 8 -0.4227710669698723 2212 9 -0.9353167003851013 2212 10 -0.3151645151842999 2212 11 -0.4329180049048347 2212 12 -0.0598667337965324 2212 13 -0.48669315384777434 2212 14 -0.16088359164843524 2212 15 -0.06327501730094554 2212 16 -1.2427562439705753 2212 17 -1.3939114567796338 2212 18 -0.36239037761017556 2212 19 -0.3112319485210264 2212 20 -0.24667496197643857 2212 21 -0.05045641741575935 2212 22 -0.37292685198595166 2212 23 -0.20213111372093806 2212 24 -0.23765535806732885 2212 25 -0.18971848614806675 2213 1 -3.0567842096423696 2213 2 -1.2660232505913989 2213 3 -2.1778928793182253 2213 4 -0.8485083501355035 2213 5 -0.6716463460694797 2213 6 -0.6140940138934212 2213 7 -0.27023411607002856 2213 8 -0.08609658505552176 2213 9 -0.15941127217305961 2213 10 -0.14094504178131473 2213 11 -0.34176527496695286 2213 12 -0.18281179409823523 2213 13 -0.5346716225105723 2213 14 -0.16504709454591193 2213 15 -0.21846583815193554 2213 16 -1.5518916817615818 2213 17 -0.8810780284898243 2213 18 -0.21493286759461228 2213 19 -0.23171668136003376 2213 20 -0.09572902536709194 2213 21 0.030310400382724367 2213 22 -0.018055723719022865 2213 23 0.15661861330089977 2213 24 0.10609388462440705 2213 25 0.12692257525353057 2214 1 -0.8123974610495139 2214 2 -0.5320767707749816 2214 3 -6.3814937430010765 2214 4 -2.1525780850787584 2214 5 -1.0625938047437984 2214 6 -0.6792107200876742 2214 7 -0.4751836717398561 2214 8 -0.315550411691639 2214 9 -0.2200638311037082 2214 10 -0.1590986680589026 2214 11 -0.6743669254873836 2214 12 -0.465529018933033 2214 13 -4.882440231349413 2214 14 -3.6669636299646484 2214 15 -2.009481534605335 2214 16 -2.7710791999956257 2214 17 -3.1583767149706077 2214 18 -1.0570003859474966 2214 19 -0.6510746036928268 2214 20 -0.3404538987014689 2214 21 -0.2553062568779154 2214 22 -0.2729428170756165 2214 23 -0.12357843779452082 2214 24 -0.17380120803096588 2214 25 0.10368793181797997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1279.0 3 189.0 4 170.0 5 368.0 6 56.0 7 64.0 8 128.0 9 353.0 10 797.0 11 1912.0 12 4532.0 13 8757.0 14 14315.0 15 21512.0 16 27794.0 17 31093.0 18 31559.0 19 30767.0 20 29313.0 21 27868.0 22 26314.0 23 25590.0 24 26239.0 25 27619.0 26 29503.0 27 33107.0 28 38274.0 29 47485.0 30 59081.0 31 75377.0 32 100206.0 33 135843.0 34 187205.0 35 261498.0 36 377848.0 37 586297.0 38 1120283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.872131872531014 22.372464441138604 12.626624796185485 21.128778890144897 2 14.34046970700264 23.469946612937303 41.012341700808186 21.177241979251875 3 17.621850743167954 31.05845365351565 29.744482898638314 21.575212704678087 4 11.29761486731722 18.551274598617255 38.4254524316078 31.72565810245772 5 13.140608849321463 39.502824298617014 33.07130782302538 14.285259029036142 6 28.88709972077369 40.16738822019714 17.82733537349655 13.118176685532617 7 23.449780783042502 35.947655901386604 24.56915073202173 16.03341258354917 8 22.91643610966752 38.487008979992225 22.34931753652687 16.247237373813388 9 24.215794806170642 17.867987735685066 22.453441436538604 35.462776021605684 10 15.94456880866618 31.064664312518353 31.04556944466989 21.945197434145577 11 30.952494780959665 29.69284243367525 21.55479889061824 17.799863894746846 12 19.501967664492177 30.484412880492602 32.21161947567312 17.801999979342106 13 25.157945248008275 27.157729224238576 27.250867341337624 20.43345818641553 14 19.50205595296733 24.700652480645296 28.740873785175715 27.05641778121166 15 23.586286642509354 30.63565527251349 24.15638780691228 21.621670278064872 16 21.216354760399682 33.19707907704784 25.486274530635516 20.10029163191696 17 18.861995876527192 31.672414698828888 29.59373817659395 19.871851248049968 18 19.366488492416643 30.983179827016304 30.120565199399703 19.529766481167353 19 19.981217426002296 29.00237202305499 30.62149837364277 20.394912177299943 20 22.778386217410976 27.88582622905052 28.638560001935986 20.697227551602516 21 23.175349212214076 30.130376888307573 27.679546369408857 19.014727530069493 22 20.72095815310806 29.268712341574904 30.038850745140998 19.97147876017604 23 20.29534147751604 30.32267337278421 29.716862105999116 19.665123043700632 24 20.484598011100342 27.8496129284381 29.58429210827378 22.08149695218778 25 21.845952788384746 27.976344018742505 28.271335450857286 21.906367742015455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4555.0 1 4555.0 2 5058.0 3 5561.0 4 5561.0 5 5561.0 6 8690.5 7 11820.0 8 11820.0 9 11820.0 10 16505.5 11 21191.0 12 21191.0 13 21191.0 14 30462.5 15 39734.0 16 39734.0 17 39734.0 18 59682.0 19 79630.0 20 79630.0 21 79630.0 22 108910.5 23 138191.0 24 138191.0 25 138191.0 26 179207.0 27 220223.0 28 220223.0 29 220223.0 30 262199.5 31 304176.0 32 304176.0 33 304176.0 34 343977.5 35 383779.0 36 383779.0 37 383779.0 38 408278.5 39 432778.0 40 432778.0 41 432778.0 42 418133.5 43 403489.0 44 403489.0 45 403489.0 46 413802.5 47 424116.0 48 424116.0 49 424116.0 50 379966.0 51 335816.0 52 335816.0 53 335816.0 54 284079.0 55 232342.0 56 232342.0 57 232342.0 58 192173.5 59 152005.0 60 152005.0 61 152005.0 62 128906.0 63 105807.0 64 105807.0 65 105807.0 66 79459.0 67 53111.0 68 53111.0 69 53111.0 70 39516.5 71 25922.0 72 25922.0 73 25922.0 74 18324.5 75 10727.0 76 10727.0 77 10727.0 78 7264.5 79 3802.0 80 3802.0 81 3802.0 82 2554.5 83 1307.0 84 1307.0 85 1307.0 86 832.5 87 358.0 88 358.0 89 358.0 90 236.5 91 115.0 92 115.0 93 115.0 94 70.5 95 26.0 96 26.0 97 26.0 98 20.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09803589045580495 2 0.05187880003362241 3 0.054828134884880086 4 0.04969629224369174 5 0.047543277802273645 6 0.06795267497297672 7 0.06403005962080402 8 0.060815284632933155 9 0.06571118048602088 10 0.06636003415329758 11 0.06019592431416905 12 0.060756297935908005 13 0.061316671557646966 14 0.0631747525139393 15 0.06633054080478501 16 0.0612576848606218 17 0.0645609398940304 18 0.14622802192535528 19 0.0692503823075301 20 0.06329272590798961 21 0.06226045871004942 22 0.06615358071370954 23 0.06624206075924727 24 0.07641726599608624 25 0.06963379583819358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3390595.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.56674009579557 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5869016909197 45.23991744575013 2 13.425090552471755 15.456773971933243 3 3.992973898510784 6.895874718745978 4 1.5610639021279034 3.594614397069019 5 0.7386863225670641 2.12618817717686 6 0.4194900076480931 1.4489203345836643 7 0.26166461377584516 1.0544225169450565 8 0.1716154726439973 0.7903474648091287 9 0.1289318437069255 0.6679967343073485 >10 0.6147446565769501 6.478215947183361 >50 0.05059344152066046 2.0004191577352537 >100 0.03942741121129646 4.81200943793601 >500 0.004173342637486567 1.7185077438972498 >1k 0.00412117585213204 4.678935760985787 >5k 3.130006976302815E-4 1.35747847119053 >10k+ 2.08667131753521E-4 1.6793777197512734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 17527 0.5169299193799318 No Hit GTACATGGGAAGCAGTGGTATCAAC 14386 0.4242913117019284 No Hit CCCATGTACTCTGCGTTGATACCAC 13398 0.3951518833715026 No Hit GGTATCAACGCAGAGTACTTTTTTT 10611 0.31295392106695136 No Hit ATTCCATTCCATTCCATTCCATTCC 9999 0.2949039917772544 No Hit TATCAACGCAGAGTACTTTTTTTTT 9859 0.2907749229854937 No Hit GAATGGAATGGAATGGAATGGAATG 7363 0.21715952509810227 No Hit GAGTACATGGGAAGCAGTGGTATCA 6593 0.1944496467434182 No Hit GTACTGGTTCACTATCGGTCAGTCA 6026 0.1777269181367872 No Hit CATGTACTCTGCGTTGATACCACTG 5363 0.15817282807294883 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4814 0.14198097973954424 No Hit GTTAATGATAGTGTGTCGAAACACA 4636 0.13673116370430557 No Hit GTATCAACGCAGAGTACATGGGAAG 4237 0.12496331764778748 No Hit GCGTTGATACCACTGCTTCCCATGT 4204 0.12399003714687244 No Hit GGATACCACGTGTCCCGCCCTACTC 3969 0.11705910024641691 No Hit GGTATCAACGCAGAGTACATGGGAA 3806 0.11225168443886693 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3494 0.103049759702943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.89866970251534E-5 0.0 5 0.0 0.0 0.0 5.89866970251534E-5 0.0 6 0.0 0.0 0.0 5.89866970251534E-5 0.0 7 0.0 0.0 0.0 8.84800455377301E-5 0.0 8 0.0 0.0 0.0 8.84800455377301E-5 0.0 9 0.0 0.0 0.0 8.84800455377301E-5 0.0 10 0.0 0.0 0.0 8.84800455377301E-5 0.0 11 0.0 0.0 0.0 1.179733940503068E-4 0.0 12 0.0 0.0 0.0 1.179733940503068E-4 0.0 13 0.0 0.0 0.0 1.179733940503068E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCC 240 0.0 14.25741 18 GTCGTAT 40 0.005282249 14.249413 1 GGACCGT 80 2.0066327E-6 13.059455 6 ATCGCCG 255 0.0 13.037139 19 AAATCGC 270 0.0 12.671944 17 TGTCCCG 785 0.0 12.097662 11 CGGAAAT 315 0.0 12.068339 14 GAAATCG 300 0.0 11.721548 16 GTCCCGC 820 0.0 11.70523 12 ACCGCTC 65 8.0464093E-4 11.689065 8 CGCCCTA 850 0.0 11.5165615 16 GTAGGAC 160 4.5474735E-11 11.278288 3 TATACCG 85 5.339586E-5 11.173866 5 CCGCCCT 890 0.0 11.105748 15 AGTGCGA 60 0.005890878 11.080096 10 TCCCGCC 875 0.0 11.078735 13 CCCGCCC 885 0.0 11.060941 14 TGGACCG 90 9.544283E-5 10.553096 5 GTACTGG 1610 0.0 10.3846655 1 GTGTCCC 945 0.0 10.250974 10 >>END_MODULE