FastQCFastQC Report
Wed 25 May 2016
SRR1294676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390595
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT481881.421225478124046No Hit
GGTATCAACGCAGAGTACTTTTTTT321240.947444327618014No Hit
TATCAACGCAGAGTACTTTTTTTTT294860.8696408742418366No Hit
ACGCAGAGTACTTTTTTTTTTTTTT175490.5175787730472085No Hit
CCCATGTACTCTGCGTTGATACCAC153240.4519560726067254No Hit
GTACATGGGAAGCAGTGGTATCAAC138600.40877781038431305No Hit
GTACTTTTTTTTTTTTTTTTTTTTT99350.2930164174724495No Hit
ATTCCATTCCATTCCATTCCATTCC92340.27234158016513327No Hit
GAGTACTTTTTTTTTTTTTTTTTTT90620.2672687242209701No Hit
GAATGGAATGGAATGGAATGGAATG82810.24423441903264767No Hit
GAGTACATGGGAAGCAGTGGTATCA67110.19792986186790226No Hit
CATGTACTCTGCGTTGATACCACTG59750.17622275736264578No Hit
GCAGAGTACTTTTTTTTTTTTTTTT56940.16793512643061173No Hit
GTACTGGTTCACTATCGGTCAGTCA56460.16651944570200805No Hit
ATCAACGCAGAGTACTTTTTTTTTT48710.14366210060476112No Hit
GTTAATGATAGTGTGTCGAAACACA46140.13608231003702892No Hit
GCGTTGATACCACTGCTTCCCATGT45970.1355809231123151No Hit
ACGCAGAGTACATGGGAAGCAGTGG43380.12794214584755773No Hit
GTATCAACGCAGAGTACATGGGAAG42910.12655595846746662No Hit
GGTATCAACGCAGAGTACATGGGAA40880.12056880871941356No Hit
GGATACCACGTGTCCCGCCCTACTC40200.11856326102055834No Hit
TATCAACGCAGAGTACATGGGAAGC35250.10396405350683287No Hit
ACTCTGCGTTGATACCACTGCTTCC34590.1020174925050028No Hit
GTGGTATCAACGCAGAGTACTTTTT34360.10133914548921355No Hit
GGTATCAACGCAGAGTACATGGGGG34160.10074927851896201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG853.8421513E-612.32381254
TTATACG802.8000117E-511.90315310
GCGGTAT657.976053E-411.7013081
AATCGCC2700.011.58766118
CGATAGT752.1145213E-411.37697518
AAATCGC2950.011.24842317
TGTCCCG7100.011.22468211
ATCGCCG2800.011.17414619
ACGGGTG909.291683E-510.5810515
TTAGACT2350.010.5360254
CGCCCTA7600.010.22929916
CGGTATC750.002649257210.1331912
AAGGTCG750.002694404510.11256915
CTTAGAC1606.182745E-910.0936093
GAAATCG3300.010.05540816
CCCCGTA9050.09.95194215
GTACTAG2201.8189894E-129.9394641
CCGTACA9350.09.8357117
CTAGGAC1752.577508E-99.7712923
GGTATCA111850.09.7410651
Produced by FastQC (version 0.11.5)