##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3390595 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22888195139791 31.0 31.0 34.0 28.0 34.0 2 31.394393314447758 31.0 31.0 34.0 28.0 34.0 3 31.461817763548876 31.0 31.0 34.0 28.0 34.0 4 35.00147437249214 37.0 35.0 37.0 32.0 37.0 5 35.202021769040535 37.0 35.0 37.0 32.0 37.0 6 34.6985555632566 37.0 35.0 37.0 32.0 37.0 7 34.55785990364523 37.0 35.0 37.0 31.0 37.0 8 34.589879062524425 37.0 35.0 37.0 31.0 37.0 9 36.01997083107832 39.0 35.0 39.0 30.0 39.0 10 35.816195387535224 39.0 35.0 39.0 30.0 39.0 11 35.955114367832195 39.0 35.0 39.0 30.0 39.0 12 35.92956457494923 39.0 35.0 39.0 30.0 39.0 13 35.75822886543512 39.0 35.0 39.0 30.0 39.0 14 36.776963335343794 40.0 36.0 41.0 30.0 41.0 15 36.81414353527921 39.0 36.0 41.0 30.0 41.0 16 36.86465207434093 39.0 36.0 41.0 30.0 41.0 17 36.95984244653224 40.0 36.0 41.0 30.0 41.0 18 36.98791628018091 40.0 36.0 41.0 30.0 41.0 19 37.02911111471585 40.0 36.0 41.0 30.0 41.0 20 37.10120731022136 40.0 37.0 41.0 30.0 41.0 21 36.988116540017316 40.0 36.0 41.0 30.0 41.0 22 36.96619413406791 40.0 36.0 41.0 30.0 41.0 23 36.976638613576675 40.0 36.0 41.0 30.0 41.0 24 36.896161293224345 40.0 36.0 41.0 30.0 41.0 25 35.806456683856375 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5173707608026312 1101 2 0.5076017714019017 1101 3 0.4183070333122032 1101 4 0.34159426553608085 1101 5 0.3779965687856759 1101 6 0.43146869170087143 1101 7 0.5242876165856387 1101 8 0.5066998984611146 1101 9 0.688800794581141 1101 10 0.6858851697838091 1101 11 0.6407381486694064 1101 12 0.8000346331598323 1101 13 0.7682057522303793 1101 14 0.9872458754846676 1101 15 0.9148023744538918 1101 16 0.9151475230680717 1101 17 0.9627581380498356 1101 18 0.9902373899521919 1101 19 1.0039199292410643 1101 20 0.9468915424139368 1101 21 1.2064384852679808 1101 22 1.157927283937589 1101 23 1.1439067611794869 1101 24 1.2426340695212872 1101 25 1.1010825547726384 1102 1 0.34584473444147434 1102 2 0.3673715185645605 1102 3 0.25852055553189146 1102 4 0.2605584999663293 1102 5 0.3191258574098157 1102 6 0.34657097239767864 1102 7 0.34739702933363503 1102 8 0.3895427659889137 1102 9 0.5170184761470864 1102 10 0.6851391135906582 1102 11 0.5684005958279315 1102 12 0.6193817192805398 1102 13 0.7154962036849213 1102 14 0.8032212113081911 1102 15 0.8142301287547014 1102 16 0.772095958474651 1102 17 0.7395264734217193 1102 18 0.761932967882764 1102 19 0.8653446774001168 1102 20 0.770175363613788 1102 21 0.9789859701411743 1102 22 0.9551387012234827 1102 23 0.8562762278876903 1102 24 0.9942102193973597 1102 25 1.0550027727905302 1103 1 0.29082254558370124 1103 2 0.21148634393800947 1103 3 0.2354260291060264 1103 4 0.18612750145248924 1103 5 0.18388036285614362 1103 6 0.26952522396261713 1103 7 0.24510620872255373 1103 8 0.3793571264131046 1103 9 0.2459969990900035 1103 10 0.534075237171102 1103 11 0.5165770831137593 1103 12 0.5216047998051181 1103 13 0.6654208167727163 1103 14 0.6599039007478353 1103 15 0.6846599529328685 1103 16 0.6016174423294416 1103 17 0.5520830111867525 1103 18 0.5029736386900865 1103 19 0.6378288487611954 1103 20 0.6381992559646719 1103 21 0.824373782741155 1103 22 0.7388155175816564 1103 23 0.7316201959452968 1103 24 0.8738925817241352 1103 25 0.823556727326725 1104 1 0.1466559887470531 1104 2 0.14355361613183248 1104 3 0.0834882390894407 1104 4 0.0014302064069582343 1104 5 0.04611078666158619 1104 6 0.07590881311161013 1104 7 0.05767432275818152 1104 8 0.01887663738063594 1104 9 -0.1364355797569985 1104 10 0.29020936738302794 1104 11 0.30658476909624 1104 12 0.32739968592163393 1104 13 -0.2172129876219202 1104 14 0.16225292119825951 1104 15 0.4037075578544389 1104 16 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2207 2 0.18282616979865196 2207 3 0.1827300296359553 2207 4 0.19202795132643757 2207 5 0.04399755299667163 2207 6 0.2209484683317271 2207 7 0.22148261524275625 2207 8 0.16367282809687111 2207 9 0.36915609132987726 2207 10 0.4549850500932635 2207 11 0.41851505209983486 2207 12 0.34160044100831755 2207 13 0.372451773047068 2207 14 0.4065764409384087 2207 15 0.45181275656839404 2207 16 0.4303479947594653 2207 17 0.5371500627297507 2207 18 0.45561158115712885 2207 19 0.6228569243411073 2207 20 0.4901985940063227 2207 21 0.8489155721402852 2207 22 0.6998003298425388 2207 23 0.5527436758205653 2207 24 0.7851038004713047 2207 25 0.7984499899336086 2208 1 0.10708382399864291 2208 2 0.10291049276476372 2208 3 0.16932549989698842 2208 4 0.02723349620154636 2208 5 0.006065737084448131 2208 6 0.17517720223342081 2208 7 0.15441363130792496 2208 8 0.09185725542799617 2208 9 0.1749664924629286 2208 10 0.2350994076589572 2208 11 -0.08739062415687471 2208 12 -0.24240162667803844 2208 13 0.014190966385761783 2208 14 -0.4123604574034019 2208 15 0.059891559798955996 2208 16 0.15667791579868862 2208 17 0.1598269341161398 2208 18 0.2319345266020747 2208 19 0.4259495327103622 2208 20 0.29821016157684 2208 21 0.5410561389592559 2208 22 0.5134298501779 2208 23 0.5814998066192345 2208 24 0.6046841407052597 2208 25 0.5007717186830618 2209 1 -0.024406272226521253 2209 2 -0.056040924229208144 2209 3 0.022052087014863275 2209 4 -0.5456701964951947 2209 5 -0.08845912315758397 2209 6 0.04180174238863543 2209 7 0.09413034451909397 2209 8 0.0860294228090126 2209 9 0.17308199999716578 2209 10 0.3116170199009076 2209 11 0.2116710809152309 2209 12 0.19858641113501818 2209 13 0.2275727607635858 2209 14 0.20506566874963283 2209 15 -0.3995984169536797 2209 16 -0.30313594351245143 2209 17 0.05302739159636616 2209 18 -0.33172417025131296 2209 19 0.0837074066967105 2209 20 0.20529015341288925 2209 21 0.4669202613588581 2209 22 0.5220868171686348 2209 23 0.46719608256095313 2209 24 0.5654230409606313 2209 25 0.539438567047533 2210 1 -0.19417215966053902 2210 2 -0.19165421492836288 2210 3 -0.12828554238117462 2210 4 -0.02806723462764893 2210 5 -0.04939241503195291 2210 6 -0.48906555791462836 2210 7 -0.17484778179689897 2210 8 -0.30251072383167354 2210 9 -0.11463076283470741 2210 10 0.22309221634131404 2210 11 0.10567173218005621 2210 12 0.1086041372903992 2210 13 0.13260080269263597 2210 14 0.08318949557521194 2210 15 -0.04075468133782323 2210 16 0.10107210050586701 2210 17 -0.9219824426657155 2210 18 -1.454821220135912 2210 19 -0.30506953754914434 2210 20 -0.1275047030640195 2210 21 -0.32123916467932645 2210 22 -0.36522017013578534 2210 23 -0.19863626326556982 2210 24 0.2450945602847483 2210 25 0.23032489930032085 2211 1 -0.2148025754124241 2211 2 -0.20488989753568632 2211 3 -0.12995556386321994 2211 4 -0.09277568688419535 2211 5 -0.08646537248499442 2211 6 -0.054759013680047985 2211 7 -0.13486708554429327 2211 8 -0.09474249339517371 2211 9 -0.011436181727844996 2211 10 0.08866227179569108 2211 11 -0.1855949344275203 2211 12 -0.040092287856992925 2211 13 -0.030365986091254626 2211 14 -0.08938750869642575 2211 15 -0.07812279163660207 2211 16 -0.23062677065795611 2211 17 -0.33282444118096066 2211 18 -0.3432791484465696 2211 19 -0.15853089791379915 2211 20 -0.24580238992756875 2211 21 0.05419542393361354 2211 22 0.019372049385339096 2211 23 -0.672130225083265 2211 24 -0.08909747241175126 2211 25 -0.7443951096272841 2212 1 -0.20906328582498546 2212 2 -0.31913470628554563 2212 3 -0.21088604918019982 2212 4 -0.11134492492045922 2212 5 -0.1452034483633966 2212 6 -0.1813534563277912 2212 7 -0.1896833407435352 2212 8 -0.34673018422957824 2212 9 -0.1875965423106294 2212 10 -0.5102269923035152 2212 11 -0.23459558838170125 2212 12 -0.369845162551826 2212 13 -0.3289062365798472 2212 14 -0.28165445802908806 2212 15 -0.18935288182650822 2212 16 -0.37891943334648204 2212 17 -0.6206144059259273 2212 18 -0.3686201959226878 2212 19 -0.5209106789972822 2212 20 -0.6757627411924432 2212 21 -0.12670299280353703 2212 22 -0.3657228963956385 2212 23 -0.3801992986957927 2212 24 -0.4111968727667161 2212 25 -0.23465629341696825 2213 1 -0.18322720882240162 2213 2 -0.19161222312686732 2213 3 -0.10637316895069304 2213 4 -0.048601505647177135 2213 5 -0.08420861698144932 2213 6 -0.2505432918534538 2213 7 -0.374971787960412 2213 8 -0.3304553735957043 2213 9 -0.28218582865109454 2213 10 -0.22445299982295808 2213 11 0.017468890362806633 2213 12 0.11080736028720395 2213 13 0.1287359195119322 2213 14 -0.6026063003409305 2213 15 -0.22707514854692334 2213 16 -0.2916731468042997 2213 17 -0.20875585253487117 2213 18 -0.1108438161634524 2213 19 -0.26617082328251485 2213 20 -0.3007884072157694 2213 21 -0.042640647194602366 2213 22 -0.22812238922043093 2213 23 -0.2882945551424996 2213 24 -0.5194822504764929 2213 25 -0.391527444598438 2214 1 -0.17144864761165834 2214 2 -0.06947263401690762 2214 3 -0.02525196903544824 2214 4 0.046642054661965915 2214 5 -0.03725992930285571 2214 6 0.09932523737309396 2214 7 0.08119456179846196 2214 8 0.026599246526281206 2214 9 0.10072676849419082 2214 10 0.12302562863203548 2214 11 0.22707489216750076 2214 12 0.0038559000135904853 2214 13 0.04260849565012137 2214 14 0.0484636836344734 2214 15 0.21967626333163537 2214 16 -0.06153522459406702 2214 17 -0.23926340456002748 2214 18 -0.14063960237613315 2214 19 -0.3289091889222462 2214 20 -0.24092602858179646 2214 21 -0.33009380045967873 2214 22 -0.5264505497048475 2214 23 -0.6872514335830573 2214 24 -0.247460687004029 2214 25 -0.5623166982206911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 26.0 10 105.0 11 256.0 12 618.0 13 1664.0 14 3682.0 15 6900.0 16 10356.0 17 13535.0 18 15889.0 19 17057.0 20 17754.0 21 18539.0 22 19485.0 23 20776.0 24 22277.0 25 24810.0 26 27440.0 27 31325.0 28 36814.0 29 45646.0 30 58351.0 31 75077.0 32 100886.0 33 136450.0 34 185864.0 35 260164.0 36 371871.0 37 589367.0 38 1277608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08736747976936 22.284329791784817 11.886707841879389 22.741594886566432 2 14.086642609925395 23.557841617769153 39.893116104990426 22.46239966731503 3 17.113221455420913 30.889301822288477 30.34687389990526 21.65060282238535 4 11.121126866917546 18.146465090404323 38.86754641447413 31.864861628204 5 13.149914303498363 38.39244572800727 33.5789573051474 14.878682663346973 6 29.385256443536168 39.943582186059025 17.014369050259816 13.656792320144989 7 23.200243081829193 36.05635132404371 24.407539666735993 16.335865927391104 8 23.59349731640993 37.67905596157012 22.002282098799302 16.72516462322065 9 24.671797340166364 15.868722475275677 23.325364214112714 36.134115970445244 10 16.35483135487577 29.999437405432296 31.409062510178522 22.236668729513415 11 31.456841262777164 28.134779493269523 21.723917711484955 18.68446153246836 12 20.268162666042187 29.653973952339353 32.583141280156575 17.494722101461885 13 26.154775875488447 25.786492215373535 27.79572776797451 20.263004141163506 14 20.0666969861598 23.307196042657285 30.176448832316094 26.449658138866816 15 24.03522489077753 28.934891818659885 25.711557127634194 21.318326162928393 16 21.001541322847856 31.93316569309094 26.761240065899955 20.30405291816124 17 18.439654396942128 30.63804435504683 30.684614352348188 20.237686895662858 18 18.940525637596085 29.443839665521043 32.05500994370591 19.56062475317696 19 19.429849255746145 27.412443368783546 33.45766811961504 19.700039255855273 20 21.970325492311506 26.228231276767122 31.661892173833028 20.139551057088344 21 22.363803779051764 28.677874562096346 30.271109477830677 18.687212181021216 22 19.851832757065125 28.094026443941928 32.61451948138516 19.439621317607795 23 19.491074275207907 29.043385626054203 32.365754309054495 19.0997857896834 24 19.677165807181336 26.493756995453598 32.55726502280573 21.271812174559333 25 21.17205864569846 26.519452710933102 31.134719350661992 21.173769292706453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3631.0 1 3631.0 2 3935.5 3 4240.0 4 4240.0 5 4240.0 6 9995.5 7 15751.0 8 15751.0 9 15751.0 10 19106.0 11 22461.0 12 22461.0 13 22461.0 14 27604.0 15 32747.0 16 32747.0 17 32747.0 18 52418.5 19 72090.0 20 72090.0 21 72090.0 22 105107.0 23 138124.0 24 138124.0 25 138124.0 26 183763.0 27 229402.0 28 229402.0 29 229402.0 30 274414.0 31 319426.0 32 319426.0 33 319426.0 34 356125.0 35 392824.0 36 392824.0 37 392824.0 38 408230.5 39 423637.0 40 423637.0 41 423637.0 42 409651.5 43 395666.0 44 395666.0 45 395666.0 46 408122.0 47 420578.0 48 420578.0 49 420578.0 50 377930.5 51 335283.0 52 335283.0 53 335283.0 54 283272.5 55 231262.0 56 231262.0 57 231262.0 58 191264.0 59 151266.0 60 151266.0 61 151266.0 62 129032.5 63 106799.0 64 106799.0 65 106799.0 66 80166.5 67 53534.0 68 53534.0 69 53534.0 70 39771.0 71 26008.0 72 26008.0 73 26008.0 74 18306.5 75 10605.0 76 10605.0 77 10605.0 78 7139.0 79 3673.0 80 3673.0 81 3673.0 82 2422.5 83 1172.0 84 1172.0 85 1172.0 86 738.0 87 304.0 88 304.0 89 304.0 90 192.0 91 80.0 92 80.0 93 80.0 94 54.5 95 29.0 96 29.0 97 29.0 98 16.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09679716981827673 2 0.0 3 0.13339841532238442 4 0.011236965783291723 5 0.021382677671618108 6 0.015660968060178228 7 0.023299745324935592 8 0.02161862445971872 9 2.064534395880369E-4 10 0.3947684698408392 11 0.06273235228625064 12 0.0012092272890156448 13 5.89866970251534E-5 14 0.0010912538949653379 15 0.0011207472434779145 16 2.94933485125767E-5 17 0.0 18 2.359467881006136E-4 19 5.308802732263806E-4 20 2.359467881006136E-4 21 2.654401366131903E-4 22 0.0030083215482828237 23 5.89866970251534E-5 24 0.0 25 0.0019170676533174858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3390595.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.62693660410118 #Duplication Level Percentage of deduplicated Percentage of total 1 77.052783469201 41.32104734272378 2 14.19469330675808 15.2243583615235 3 4.424844940275915 7.118726372854632 4 1.7297485262585375 3.7104445823481615 5 0.8147848961694999 2.184720898643047 6 0.4307968540286068 1.3861389348142998 7 0.29129819035226917 1.0935000710837377 8 0.16695529784908283 0.7162640938777277 9 0.12731789252096812 0.6144901695710754 >10 0.6592406934556713 6.470315479725934 >50 0.05362062775573905 1.9644814339228747 >100 0.0440138697940638 5.033680257010752 >500 0.004450083407720141 1.6562812165868288 >1k 0.0046725875767509 5.030264735857111 >5k 4.4500834064294283E-4 1.805881387310042 >10k+ 3.337562554822071E-4 4.6694046621465555 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 48188 1.421225478124046 No Hit GGTATCAACGCAGAGTACTTTTTTT 32124 0.947444327618014 No Hit TATCAACGCAGAGTACTTTTTTTTT 29486 0.8696408742418366 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 17549 0.5175787730472085 No Hit CCCATGTACTCTGCGTTGATACCAC 15324 0.4519560726067254 No Hit GTACATGGGAAGCAGTGGTATCAAC 13860 0.40877781038431305 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 9935 0.2930164174724495 No Hit ATTCCATTCCATTCCATTCCATTCC 9234 0.27234158016513327 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 9062 0.2672687242209701 No Hit GAATGGAATGGAATGGAATGGAATG 8281 0.24423441903264767 No Hit GAGTACATGGGAAGCAGTGGTATCA 6711 0.19792986186790226 No Hit CATGTACTCTGCGTTGATACCACTG 5975 0.17622275736264578 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 5694 0.16793512643061173 No Hit GTACTGGTTCACTATCGGTCAGTCA 5646 0.16651944570200805 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4871 0.14366210060476112 No Hit GTTAATGATAGTGTGTCGAAACACA 4614 0.13608231003702892 No Hit GCGTTGATACCACTGCTTCCCATGT 4597 0.1355809231123151 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4338 0.12794214584755773 No Hit GTATCAACGCAGAGTACATGGGAAG 4291 0.12655595846746662 No Hit GGTATCAACGCAGAGTACATGGGAA 4088 0.12056880871941356 No Hit GGATACCACGTGTCCCGCCCTACTC 4020 0.11856326102055834 No Hit TATCAACGCAGAGTACATGGGAAGC 3525 0.10396405350683287 No Hit ACTCTGCGTTGATACCACTGCTTCC 3459 0.1020174925050028 No Hit GTGGTATCAACGCAGAGTACTTTTT 3436 0.10133914548921355 No Hit GGTATCAACGCAGAGTACATGGGGG 3416 0.10074927851896201 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.89866970251534E-5 0.0 10 0.0 0.0 0.0 5.89866970251534E-5 0.0 11 0.0 0.0 0.0 5.89866970251534E-5 0.0 12 0.0 0.0 0.0 5.89866970251534E-5 0.0 13 0.0 0.0 0.0 5.89866970251534E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACG 85 3.8421513E-6 12.3238125 4 TTATACG 80 2.8000117E-5 11.903153 10 GCGGTAT 65 7.976053E-4 11.701308 1 AATCGCC 270 0.0 11.587661 18 CGATAGT 75 2.1145213E-4 11.376975 18 AAATCGC 295 0.0 11.248423 17 TGTCCCG 710 0.0 11.224682 11 ATCGCCG 280 0.0 11.174146 19 ACGGGTG 90 9.291683E-5 10.581051 5 TTAGACT 235 0.0 10.536025 4 CGCCCTA 760 0.0 10.229299 16 CGGTATC 75 0.0026492572 10.133191 2 AAGGTCG 75 0.0026944045 10.112569 15 CTTAGAC 160 6.182745E-9 10.093609 3 GAAATCG 330 0.0 10.055408 16 CCCCGTA 905 0.0 9.951942 15 GTACTAG 220 1.8189894E-12 9.939464 1 CCGTACA 935 0.0 9.83571 17 CTAGGAC 175 2.577508E-9 9.771292 3 GGTATCA 11185 0.0 9.741065 1 >>END_MODULE