FastQCFastQC Report
Wed 25 May 2016
SRR1294675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726659
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89350.23975899055964067No Hit
CCCATGTACTCTGCGTTGATACCAC79280.21273746806455862No Hit
GTATCAACGCAGAGTACTTTTTTTT66680.1789270228373457No Hit
ATTCCATTCCATTCCATTCCATTCC65370.17541180988118313No Hit
GAATGGAATGGAATGGAATGGAATG49980.1341147660679445No Hit
GGTATCAACGCAGAGTACTTTTTTT49770.13355125864749096No Hit
GAGTACATGGGAAGCAGTGGTATCA43800.11753154769459723No Hit
TATCAACGCAGAGTACTTTTTTTTT37890.10167283886183308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCG550.003077215312.087039
CGCCCTA1850.011.81670116
CGTGCAT600.00589232811.07977710
AGCACGG951.3661453E-510.9966185
CCCCGGT1355.7134457E-810.55330319
CTGTACG750.002656116410.1300829
CCGTGCA750.002656116410.1300829
TATACCG856.616222E-410.0557325
TGTCCCG2107.2759576E-129.94825311
CCGCCCT2300.09.91758715
CTAGGAC3700.09.7540193
GTCCCGC2251.8189894E-129.71530112
TCCCGCC2450.09.6983213
CCCCCGG1401.0507938E-69.50588618
AGCGTAC1502.652887E-79.4996311
GTCTTAC3400.09.4996311
CGCAGAA900.00111714569.49733452
TGCTCGG800.00452992379.49695110
TAGGACC2451.8189894E-129.3033864
CTTACAC5950.09.2579063
Produced by FastQC (version 0.11.5)