##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294675_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3726659 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.288650236042525 31.0 30.0 34.0 26.0 34.0 2 30.297566533455303 31.0 30.0 34.0 26.0 34.0 3 30.28910775040056 31.0 30.0 34.0 26.0 34.0 4 33.93616185435802 35.0 35.0 37.0 28.0 37.0 5 34.26903588442087 37.0 35.0 37.0 30.0 37.0 6 34.37753118812319 37.0 35.0 37.0 30.0 37.0 7 34.23431148382505 37.0 35.0 37.0 30.0 37.0 8 34.15646964211107 37.0 35.0 37.0 29.0 37.0 9 35.2769437718879 39.0 35.0 39.0 28.0 39.0 10 35.35132433635597 39.0 35.0 39.0 28.0 39.0 11 35.62255736304287 39.0 35.0 39.0 29.0 39.0 12 35.38637261955011 39.0 35.0 39.0 27.0 39.0 13 35.26915663601097 39.0 35.0 39.0 27.0 39.0 14 36.19861006869692 39.0 36.0 41.0 27.0 41.0 15 36.187843856923855 39.0 36.0 41.0 27.0 41.0 16 36.30347718962213 39.0 36.0 41.0 27.0 41.0 17 36.27217569410026 39.0 36.0 41.0 27.0 41.0 18 36.16364926332138 39.0 36.0 41.0 27.0 41.0 19 36.44442327564717 39.0 36.0 41.0 27.0 41.0 20 36.55292448276057 40.0 36.0 41.0 27.0 41.0 21 36.58842276688047 40.0 36.0 41.0 28.0 41.0 22 36.57144214160727 39.0 36.0 41.0 28.0 41.0 23 36.54997277722485 39.0 36.0 41.0 28.0 41.0 24 36.60078933972762 39.0 36.0 41.0 28.0 41.0 25 35.376531096620326 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.01506641859444 1101 2 1.0169836922001139 1101 3 1.302847709743478 1101 4 1.0992952825586002 1101 5 0.8266456677872682 1101 6 0.7968528337368284 1101 7 0.788535442651785 1101 8 0.8954655532494726 1101 9 1.2023668082254204 1101 10 1.1545041564286223 1101 11 0.9599947516975078 1101 12 1.0781531590947466 1101 13 1.173217847332559 1101 14 1.2679933582617622 1101 15 1.459904488194745 1101 16 1.3976869884268197 1101 17 1.321759287691016 1101 18 1.4931587993023285 1101 19 1.1951641614279254 1101 20 1.1215958660939194 1101 21 1.120493167452075 1101 22 1.039575579012343 1101 23 1.0815160598952076 1101 24 1.1577851276711044 1101 25 1.053476446389574 1102 1 0.902753766786855 1102 2 0.8340791423209488 1102 3 1.1283390795357384 1102 4 0.9857188323847836 1102 5 0.7283911202407367 1102 6 0.6914127360745823 1102 7 0.6542994227950913 1102 8 0.725022355603123 1102 9 1.072410326733646 1102 10 0.9382019132407109 1102 11 0.7655611784233756 1102 12 0.9269329093099756 1102 13 0.9471520162812936 1102 14 1.2104232290911696 1102 15 1.2705305998425587 1102 16 1.1524242599647039 1102 17 1.1323354855816419 1102 18 1.295952278573779 1102 19 0.9451232257027655 1102 20 0.20697746819156038 1102 21 0.4883178183140373 1102 22 0.638697749123466 1102 23 0.7720816392003087 1102 24 0.8284625548012698 1102 25 0.8722073141252054 1103 1 0.7238752500768513 1103 2 0.7356563490182921 1103 3 0.9859699346153086 1103 4 0.8455841572841578 1103 5 0.5857291317222675 1103 6 0.5558253655525434 1103 7 0.5659993591870816 1103 8 0.7001574252746536 1103 9 0.7971350887656143 1103 10 0.6795329610509526 1103 11 0.6089572139734827 1103 12 0.897766126338233 1103 13 0.7437813274244434 1103 14 0.6959066390570428 1103 15 0.9563516714822171 1103 16 0.9298029443207554 1103 17 0.8677498726115829 1103 18 0.9869199945531903 1103 19 0.832801081503078 1103 20 0.02827719984215804 1103 21 0.46462092769946395 1103 22 -0.09768020299608793 1103 23 0.48256603044429625 1103 24 0.5960101957311963 1103 25 0.706725141521126 1104 1 0.3493411137288547 1104 2 0.3679368538442702 1104 3 0.6340225295046196 1104 4 0.5220996083301159 1104 5 0.3996462225957842 1104 6 0.16610697434980182 1104 7 0.25159995000356616 1104 8 0.2720585272024181 1104 9 0.5479137862850223 1104 10 0.5602831582626422 1104 11 0.30038315421969486 1104 12 0.4534108467012601 1104 13 0.35958943506864216 1104 14 0.6197844118451883 1104 15 0.6106889200570933 1104 16 0.45088430634796595 1104 17 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0.373667597820301 2207 9 -1.176201210739407 2207 10 -0.10826073389866764 2207 11 0.22337874245706502 2207 12 0.5389433468744258 2207 13 0.6524677108483345 2207 14 0.9099988085249819 2207 15 0.9181241748607718 2207 16 0.7985491537456468 2207 17 0.7525221622053522 2207 18 0.8340209728312971 2207 19 -0.3251960681081485 2207 20 0.33124912693521225 2207 21 -0.22785176375946747 2207 22 0.3051529083117117 2207 23 -0.45175265342716386 2207 24 -1.4831956599700362 2207 25 -0.21125951163303114 2208 1 0.4077815257056052 2208 2 0.1325818304262043 2208 3 0.6304816378423155 2208 4 0.4971723281250888 2208 5 0.4021921231862393 2208 6 0.2967956800944478 2208 7 0.4528025354361347 2208 8 0.48887238357264806 2208 9 -0.1442759713293853 2208 10 0.10525953430235546 2208 11 0.31905403557177436 2208 12 0.2788959498884722 2208 13 0.5769738799557587 2208 14 0.8685707852545761 2208 15 0.7880323093374457 2208 16 0.6130989927867958 2208 17 0.6415288819753968 2208 18 0.8088565976938611 2208 19 -1.2653747987107025 2208 20 -0.0339771230541146 2208 21 -0.15625705172909932 2208 22 0.25339552523148257 2208 23 0.41435495305397296 2208 24 0.5028782301983981 2208 25 0.5929868939979457 2209 1 0.12849605179227908 2209 2 -1.3731407742629358 2209 3 -0.22290959852874437 2209 4 0.1035080914258657 2209 5 0.03627675377568096 2209 6 0.17030667765379803 2209 7 0.0986078979002798 2209 8 0.2704844880294317 2209 9 -2.1762471363969382 2209 10 -0.40892180959528446 2209 11 -0.08112683594187331 2209 12 0.28397167415835156 2209 13 0.34948395295232615 2209 14 0.542475227127305 2209 15 0.6555287020097751 2209 16 0.5864593918188987 2209 17 0.31066210610767087 2209 18 0.6532792963720908 2209 19 0.31962599904693434 2209 20 0.34665596904752505 2209 21 0.33894225878534456 2209 22 0.26885676500955924 2209 23 0.3724535517177827 2209 24 0.35282200396129326 2209 25 0.330240329407097 2210 1 0.1369494421993913 2210 2 0.18015584352953695 2210 3 0.14561138438889998 2210 4 0.31044260429014514 2210 5 0.15837792062924905 2210 6 0.10935496031135727 2210 7 0.14621574689389405 2210 8 0.2137812035191189 2210 9 0.3519793662035511 2210 10 -1.3862785279181864 2210 11 -0.3093852645939492 2210 12 -2.1219170598882187 2210 13 -0.6290896887599189 2210 14 -0.48323975941490005 2210 15 -0.6950733919205021 2210 16 -5.14290647032567E-4 2210 17 0.12988385713471473 2210 18 0.45822870666477655 2210 19 0.3206325943062467 2210 20 0.26099805860934566 2210 21 0.29491703615783393 2210 22 0.2579720400376644 2210 23 0.15955797398185467 2210 24 0.14316550734127276 2210 25 0.13136799941942456 2211 1 -0.3821058106076727 2211 2 -0.10500699603111485 2211 3 -1.5413847677543124 2211 4 -0.6823978625212632 2211 5 -0.48514378554123994 2211 6 -0.46704054160815645 2211 7 -0.49243031290222206 2211 8 -0.15012906030550255 2211 9 0.0016043419840841011 2211 10 -0.0350174578706941 2211 11 -0.2724924734133012 2211 12 -0.31963589891549304 2211 13 -0.341521551931514 2211 14 -0.8131630527618583 2211 15 -0.3998350686519103 2211 16 -0.23458668310338027 2211 17 -0.31301514159740407 2211 18 -0.0100217230236197 2211 19 -0.15596399367691305 2211 20 -0.09763409202802364 2211 21 -0.2568291231683375 2211 22 -0.3711936535527016 2211 23 -0.6571544917333227 2211 24 -0.4627224944622057 2211 25 -0.24086013288555108 2212 1 -0.4373723042491555 2212 2 -0.43414835063450496 2212 3 -2.430657444035603 2212 4 -1.512298579022982 2212 5 -0.7184452708325182 2212 6 -0.5608465727717018 2212 7 -0.42961401114123277 2212 8 -0.45263087453476913 2212 9 -0.9355383974545717 2212 10 -0.3440657876706368 2212 11 -0.5367222895185861 2212 12 -0.16414781578734505 2212 13 -0.580010465327021 2212 14 -0.22651958287806195 2212 15 -0.03637705261063928 2212 16 -1.2409711856242893 2212 17 -1.4597266331949044 2212 18 -0.3664002837456337 2212 19 -0.25532259629257226 2212 20 -0.37925040392968157 2212 21 -0.13864801080020328 2212 22 -0.47954766434617824 2212 23 -0.2110706917707148 2212 24 -0.3515729069022697 2212 25 -0.16716962613725173 2213 1 -3.2174129876013424 2213 2 -1.4401971861832514 2213 3 -2.400296616058373 2213 4 -1.1067968718846117 2213 5 -0.8914821434197364 2213 6 -0.6821055936549953 2213 7 -0.4831609014298692 2213 8 -0.2888393399014575 2213 9 -0.30791871104634794 2213 10 -0.25979381832542003 2213 11 -0.5360331951182715 2213 12 -0.2447011350360313 2213 13 -0.7031811404307504 2213 14 -0.26632231773155013 2213 15 -0.33215368902750697 2213 16 -1.5945586358732697 2213 17 -1.112129386941234 2213 18 -0.3475827624638086 2213 19 -0.215827483018046 2213 20 -0.3528701885610772 2213 21 -0.170608540447013 2213 22 -0.13781428845491916 2213 23 -0.040978634920250556 2213 24 -0.08029330981667471 2213 25 -0.07558509676108827 2214 1 -0.9201982304534155 2214 2 -0.6762814987286134 2214 3 -6.469765441041325 2214 4 -2.1110764035389096 2214 5 -1.2027947685167035 2214 6 -0.65544313579354 2214 7 -0.5623698870537268 2214 8 -0.2958348901333778 2214 9 -0.2489363920951888 2214 10 -0.11233506317185515 2214 11 -0.7433998886828945 2214 12 -0.47062540603779013 2214 13 -5.047020136870614 2214 14 -3.6824607715387145 2214 15 -2.2097071993998725 2214 16 -2.8119238435664258 2214 17 -3.460494437420401 2214 18 -1.1771302593496245 2214 19 -0.8354231082542327 2214 20 -0.45851544436902714 2214 21 -0.5121394448686729 2214 22 -0.4551592970051459 2214 23 -0.33492250299327253 2214 24 -0.34853743492450207 2214 25 -0.041533252622883765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1485.0 3 161.0 4 193.0 5 441.0 6 56.0 7 73.0 8 183.0 9 384.0 10 956.0 11 2086.0 12 4152.0 13 7293.0 14 11179.0 15 14937.0 16 18783.0 17 21164.0 18 22728.0 19 23964.0 20 24602.0 21 25271.0 22 26227.0 23 27542.0 24 29725.0 25 32239.0 26 36009.0 27 40976.0 28 47791.0 29 58442.0 30 72957.0 31 90746.0 32 118556.0 33 157913.0 34 212997.0 35 291208.0 36 411680.0 37 627331.0 38 1264229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44489027478914 22.19887716293601 12.979778349675025 22.376454212599832 2 14.484293988631777 22.221327269852033 41.6879642956962 21.606414445819986 3 17.664212606458957 28.501627090247123 30.516668277647106 23.317492025646807 4 11.367747810925852 17.712585988314554 40.32398888051819 30.5956773202414 5 12.350620927245625 39.08469610862683 35.50563903314905 13.059043930978492 6 29.37997928600909 40.0602784117197 18.169263434220724 12.390478868050483 7 24.222921193361053 33.89070523526638 25.241205906013743 16.645167665358827 8 22.64279724695978 38.667471825050434 22.41164089327472 16.27809003471507 9 23.770260919206585 17.042412629296237 23.332195699205958 35.855130752291224 10 14.06971851540769 30.858851704833352 34.61165738087138 20.459772398887583 11 31.206224092906016 27.277098974697978 23.655757785351923 17.86091914704409 12 19.302521466444308 28.903651987312518 33.45964786412269 18.334178682120484 13 25.25096520949725 25.483947275221443 28.01428770506087 21.250799810220443 14 19.07472233641525 23.9886622660807 29.806769290916364 27.129846106587696 15 22.088973598029707 31.489701180092133 25.003168537177682 21.418156684700477 16 20.596645106410858 32.81435312117125 26.702988568830953 19.886013203586938 17 18.5164042008846 31.58837087907957 30.170255477196516 19.724969442839313 18 18.842589669002304 31.371299201225373 30.77621546089095 19.00989566888138 19 19.598903757632264 29.618495625932802 31.017101531312885 19.76549908512205 20 21.26510792641864 29.397530317888066 29.72168012278597 19.61568163290733 21 21.648952509016393 30.331189948898164 28.68155006755581 19.338307474529635 22 19.609317372775198 29.979160048731273 30.84672606540592 19.5647965130876 23 19.619722080430783 30.562157323065993 30.1677339735474 19.65038662295582 24 19.70754054514915 29.12344066155827 30.364949978987283 20.8040688143053 25 20.574237070764195 29.377911182647054 29.16845909320135 20.879392653387406 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3504.0 1 3504.0 2 3588.5 3 3673.0 4 3673.0 5 3673.0 6 6217.5 7 8762.0 8 8762.0 9 8762.0 10 14820.0 11 20878.0 12 20878.0 13 20878.0 14 34778.5 15 48679.0 16 48679.0 17 48679.0 18 74231.0 19 99783.0 20 99783.0 21 99783.0 22 136587.5 23 173392.0 24 173392.0 25 173392.0 26 219314.5 27 265237.0 28 265237.0 29 265237.0 30 312293.5 31 359350.0 32 359350.0 33 359350.0 34 397983.0 35 436616.0 36 436616.0 37 436616.0 38 459316.0 39 482016.0 40 482016.0 41 482016.0 42 466250.0 43 450484.0 44 450484.0 45 450484.0 46 438155.0 47 425826.0 48 425826.0 49 425826.0 50 384844.5 51 343863.0 52 343863.0 53 343863.0 54 297986.5 55 252110.0 56 252110.0 57 252110.0 58 211164.5 59 170219.0 60 170219.0 61 170219.0 62 136471.0 63 102723.0 64 102723.0 65 102723.0 66 76073.5 67 49424.0 68 49424.0 69 49424.0 70 34722.5 71 20021.0 72 20021.0 73 20021.0 74 13487.5 75 6954.0 76 6954.0 77 6954.0 78 4568.5 79 2183.0 80 2183.0 81 2183.0 82 1408.5 83 634.0 84 634.0 85 634.0 86 419.5 87 205.0 88 205.0 89 205.0 90 142.0 91 79.0 92 79.0 93 79.0 94 49.5 95 20.0 96 20.0 97 20.0 98 22.0 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10110933144137954 2 0.055438396697953855 3 0.05954395076125828 4 0.05433821554373502 5 0.05117184051451984 6 0.06992858750961653 7 0.06587670081968863 8 0.06279082685053824 9 0.06858690317520331 10 0.0674062209609197 11 0.06152964357618982 12 0.063273833210927 13 0.06273715947716171 14 0.06595720187975342 15 0.06823806524825587 16 0.06354217007780964 17 0.06684271354046614 18 0.14313088479520128 19 0.07151177502422411 20 0.06587670081968863 21 0.06254932367034387 22 0.0687479052953329 23 0.06676221248040135 24 0.0730949625388317 25 0.071297105530718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3726659.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.06936826434293 #Duplication Level Percentage of deduplicated Percentage of total 1 89.98503651871927 72.05045027297679 2 7.8336850539124185 12.54476426897185 3 1.1629451231856691 2.7934884401872493 4 0.357302616901843 1.144359792581084 5 0.16142620607811228 0.6462647170992041 6 0.0992601143615495 0.47686167904654203 7 0.06413269693987439 0.35945451703450104 8 0.046918612516787334 0.30053949312469236 9 0.03837176809197287 0.27651629072791245 >10 0.2156322572127939 3.2087002408806917 >50 0.019164781942362406 1.0533721544983354 >100 0.013829936861739126 2.151200681616008 >500 0.0013495960666467708 0.7459201628600659 >1k 8.097576076545579E-4 1.4358104951405684 >5k 1.3495960127575844E-4 0.8122967932544297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 8935 0.23975899055964067 No Hit CCCATGTACTCTGCGTTGATACCAC 7928 0.21273746806455862 No Hit GTATCAACGCAGAGTACTTTTTTTT 6668 0.1789270228373457 No Hit ATTCCATTCCATTCCATTCCATTCC 6537 0.17541180988118313 No Hit GAATGGAATGGAATGGAATGGAATG 4998 0.1341147660679445 No Hit GGTATCAACGCAGAGTACTTTTTTT 4977 0.13355125864749096 No Hit GAGTACATGGGAAGCAGTGGTATCA 4380 0.11753154769459723 No Hit TATCAACGCAGAGTACTTTTTTTTT 3789 0.10167283886183308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 5 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 6 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 7 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 8 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 9 0.0 2.6833686688264206E-5 0.0 8.050106006479262E-5 0.0 10 0.0 2.6833686688264206E-5 0.0 1.0733474675305682E-4 0.0 11 2.6833686688264206E-5 2.6833686688264206E-5 0.0 1.3416843344132102E-4 0.0 12 2.6833686688264206E-5 2.6833686688264206E-5 0.0 1.3416843344132102E-4 0.0 13 2.6833686688264206E-5 2.6833686688264206E-5 0.0 1.3416843344132102E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGCCG 55 0.0030772153 12.08703 9 CGCCCTA 185 0.0 11.816701 16 CGTGCAT 60 0.005892328 11.079777 10 AGCACGG 95 1.3661453E-5 10.996618 5 CCCCGGT 135 5.7134457E-8 10.553303 19 CTGTACG 75 0.0026561164 10.130082 9 CCGTGCA 75 0.0026561164 10.130082 9 TATACCG 85 6.616222E-4 10.055732 5 TGTCCCG 210 7.2759576E-12 9.948253 11 CCGCCCT 230 0.0 9.917587 15 CTAGGAC 370 0.0 9.754019 3 GTCCCGC 225 1.8189894E-12 9.715301 12 TCCCGCC 245 0.0 9.69832 13 CCCCCGG 140 1.0507938E-6 9.505886 18 AGCGTAC 150 2.652887E-7 9.499631 1 GTCTTAC 340 0.0 9.499631 1 CGCAGAA 90 0.0011171456 9.4973345 2 TGCTCGG 80 0.0045299237 9.496951 10 TAGGACC 245 1.8189894E-12 9.303386 4 CTTACAC 595 0.0 9.257906 3 >>END_MODULE