FastQCFastQC Report
Wed 25 May 2016
SRR1294675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726659
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167950.4506717679293974No Hit
GGTATCAACGCAGAGTACTTTTTTT136820.3671385012688309No Hit
TATCAACGCAGAGTACTTTTTTTTT102580.27525995804821424No Hit
CCCATGTACTCTGCGTTGATACCAC91450.2453940647641762No Hit
GTACATGGGAAGCAGTGGTATCAAC86880.23313106994763944No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68020.18252273685357312No Hit
ATTCCATTCCATTCCATTCCATTCC64160.17216493379190315No Hit
GAATGGAATGGAATGGAATGGAATG56100.1505369823211622No Hit
GAGTACATGGGAAGCAGTGGTATCA44610.11970507631634664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC402.719318E-416.6593068
TACCGTC508.567179E-515.2313657
CGAACCG604.1550488E-412.644608512
CGCCCTA1750.011.9222216
ACCATCG750.002604138610.1542438
TGTGCGA750.00260530310.15369610
GTCTCGC1502.4132532E-810.1427691
TATACCG951.611352E-410.0206355
TATTCCG2350.09.7221485
GACTGTC2650.09.6992187
TAGACTG5400.09.6958925
CTGTGCG900.00109400849.519099
GTAGGAC1701.668559E-89.49951653
CCGCCCT2201.8189894E-119.48345715
GTCCCGC2357.2759576E-129.28168112
CCCGTAC1651.09570465E-79.19620216
TCTCGCT1451.7091716E-69.1719472
TAATACT7750.08.9668514
GCGAACC850.00751690948.929811
CCCCGTA1606.9196176E-78.8907415
Produced by FastQC (version 0.11.5)