##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294675_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3726659 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34192959430954 31.0 31.0 34.0 28.0 34.0 2 31.546492448061386 31.0 31.0 34.0 28.0 34.0 3 31.62033955883809 31.0 31.0 34.0 28.0 34.0 4 35.19240477865026 37.0 35.0 37.0 32.0 37.0 5 35.297890147716764 37.0 35.0 37.0 32.0 37.0 6 34.87860010803242 37.0 35.0 37.0 32.0 37.0 7 34.72617832755828 37.0 35.0 37.0 32.0 37.0 8 34.7725096393311 37.0 35.0 37.0 32.0 37.0 9 36.27193848430994 39.0 37.0 39.0 32.0 39.0 10 36.0359407716134 39.0 35.0 39.0 30.0 39.0 11 36.18150627680182 39.0 35.0 39.0 31.0 39.0 12 36.131150180362624 39.0 35.0 39.0 30.0 39.0 13 36.0007494648692 39.0 35.0 39.0 30.0 39.0 14 37.08306260379605 40.0 36.0 41.0 30.0 41.0 15 37.14055807091553 40.0 36.0 41.0 31.0 41.0 16 37.180024520622894 40.0 37.0 41.0 31.0 41.0 17 37.21652316458254 40.0 37.0 41.0 31.0 41.0 18 37.22808982522952 40.0 37.0 41.0 31.0 41.0 19 37.257578705215586 40.0 37.0 41.0 31.0 41.0 20 37.31110090834713 40.0 37.0 41.0 31.0 41.0 21 37.16172367796463 40.0 37.0 41.0 30.0 41.0 22 37.14252605349725 40.0 37.0 41.0 30.0 41.0 23 37.155691196860246 40.0 37.0 41.0 30.0 41.0 24 37.087689268054845 40.0 36.0 41.0 30.0 41.0 25 36.01941122061342 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47469043313622805 1101 2 0.4339563289492965 1101 3 0.3942987327852414 1101 4 0.31238137507590125 1101 5 0.3482883303954125 1101 6 0.4575753778482792 1101 7 0.5004091897999032 1101 8 0.48603959651458695 1101 9 0.668444779292777 1101 10 0.6094844096200234 1101 11 0.6185489547346208 1101 12 0.6571079964178068 1101 13 0.699760287502798 1101 14 0.8239384770680616 1101 15 0.841583739684495 1101 16 0.9344739974350844 1101 17 0.9378657795818697 1101 18 0.9491968201388303 1101 19 1.073441460830722 1101 20 0.9269828552151296 1101 21 1.197804047110587 1101 22 1.1091218734732777 1101 23 1.0256594793647835 1101 24 1.2661848365608677 1101 25 1.0861139814569398 1102 1 0.41593481317621794 1102 2 0.4146196691127031 1102 3 0.3389280896474567 1102 4 0.3501174057804377 1102 5 0.3365041531853237 1102 6 0.39324064572551265 1102 7 0.48603422300983823 1102 8 0.4663732637324216 1102 9 0.5902069372324448 1102 10 0.6866021408827478 1102 11 0.6073021705934423 1102 12 0.7291602819767533 1102 13 0.7497905101265516 1102 14 0.9466965003871977 1102 15 0.8489471710264311 1102 16 0.9045988952861705 1102 17 0.8174281406017911 1102 18 0.77747875170828 1102 19 0.9606771832553207 1102 20 0.9069655638867076 1102 21 1.1346012207421339 1102 22 1.0622446536804944 1102 23 0.9091314779715702 1102 24 1.0959291084250893 1102 25 1.0115792871288107 1103 1 0.30998984882382885 1103 2 0.28933998523392646 1103 3 0.25828735044422757 1103 4 0.19867356169359596 1103 5 0.22239732841515547 1103 6 0.2838238497632375 1103 7 0.3685544496479096 1103 8 0.4094401645771981 1103 9 0.331783044662906 1103 10 0.5900264847127161 1103 11 0.5275255249890591 1103 12 0.6037073125232482 1103 13 0.6755733420298782 1103 14 0.8287282555081958 1103 15 0.721651556448073 1103 16 0.6793341213423361 1103 17 0.7301651619310405 1103 18 0.7048775883096496 1103 19 0.7819375068552432 1103 20 0.7376119671390313 1103 21 1.0229554866028039 1103 22 0.9394383202790877 1103 23 0.8781267772717953 1103 24 0.9694528046781912 1103 25 0.8557530677267167 1104 1 0.18311463809773443 1104 2 0.13671433367537844 1104 3 0.08884813495699717 1104 4 0.09695173534045409 1104 5 0.07103782103001066 1104 6 0.06061737622628982 1104 7 0.14973981175607776 1104 8 0.08989844277351011 1104 9 -0.14937264533536165 1104 10 0.21337069873138148 1104 11 0.26078671766330785 1104 12 0.34357021752908423 1104 13 -0.13452269300024966 1104 14 0.24004756370963776 1104 15 0.3638982212368873 1104 16 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0.21898594422918904 2207 4 0.14694487950939816 2207 5 0.13040507729814976 2207 6 0.2258301826359954 2207 7 0.24458705394502545 2207 8 0.23459605821651053 2207 9 0.3817259697867641 2207 10 0.5718861356598595 2207 11 0.47974711923124147 2207 12 0.37427939317547754 2207 13 0.4552095337878157 2207 14 0.5098450127750525 2207 15 0.4771636182319128 2207 16 0.5031976160671263 2207 17 0.5550483234687036 2207 18 0.559110555092488 2207 19 0.6549077744265972 2207 20 0.5106738551529233 2207 21 0.8659038370168659 2207 22 0.7752379632663704 2207 23 0.6038188797290971 2207 24 0.8139723963871219 2207 25 0.6845635452877303 2208 1 0.20184394236065017 2208 2 0.20881721359030436 2208 3 0.16543413682756025 2208 4 0.14267319652061872 2208 5 0.14120425168870554 2208 6 0.287989307800963 2208 7 0.21306690130167283 2208 8 0.17487443790613355 2208 9 0.27670204024900613 2208 10 0.35551029500076226 2208 11 -0.0025006340081290546 2208 12 -0.1545689998921631 2208 13 0.1037197605355189 2208 14 -0.3435991171741293 2208 15 0.18325020563410988 2208 16 0.2906946504044754 2208 17 0.2045352876396862 2208 18 0.42642654344106035 2208 19 0.5918420013024175 2208 20 0.3688284062660614 2208 21 0.710617049060744 2208 22 0.7186367174305559 2208 23 0.6789644150161251 2208 24 0.7678633352056821 2208 25 0.676442021233072 2209 1 -0.01465790084269969 2209 2 0.021965757456175794 2209 3 0.04570318668775997 2209 4 -0.5125916555319137 2209 5 -0.09051939949124232 2209 6 0.16202675572898784 2209 7 0.16775663628588688 2209 8 0.1418322570806012 2209 9 0.2131117685724675 2209 10 0.2731752967112371 2209 11 0.2592638177721227 2209 12 0.35314604804645455 2209 13 0.3594862593131083 2209 14 0.3311920549582794 2209 15 -0.262491301382326 2209 16 -0.24683051031408354 2209 17 0.1723308568449866 2209 18 -0.12166897566924462 2209 19 0.27739509816970553 2209 20 0.3344659539660455 2209 21 0.6092994948393482 2209 22 0.5014408947543174 2209 23 0.47614185094643346 2209 24 0.5371020096599537 2209 25 0.5315349896091206 2210 1 -0.043836044023539245 2210 2 -0.030316868002739028 2210 3 -0.0013046618871506155 2210 4 0.08485980814670313 2210 5 0.10641590890743657 2210 6 -0.41373083377386166 2210 7 -0.05195510112635304 2210 8 -0.13285903719106074 2210 9 0.13891157722550673 2210 10 0.33437813076764655 2210 11 0.25479253063235774 2210 12 0.24599168157445916 2210 13 0.3065205908094981 2210 14 0.30439953800607356 2210 15 0.17629982826954915 2210 16 0.195069344465999 2210 17 -0.6559223729200028 2210 18 -1.2249714901860003 2210 19 -0.10254902600623694 2210 20 -0.026049301103917344 2210 21 -0.2199602952930917 2210 22 -0.1499577454401333 2210 23 0.003321737324583296 2210 24 0.34487228723865115 2210 25 0.36710462364236207 2211 1 -0.22524322654220086 2211 2 -0.2099011992764659 2211 3 -0.1324099390426099 2211 4 -0.12483873588558225 2211 5 -0.11131852635345751 2211 6 -0.023810111558475455 2211 7 -0.15169603567742485 2211 8 -0.132712983657008 2211 9 -0.06592520748974096 2211 10 -0.050777562668010034 2211 11 -0.25915733156530507 2211 12 -0.1294330892522595 2211 13 -0.11451988498981791 2211 14 -0.19302649407487138 2211 15 -0.27427241426551774 2211 16 -0.1687379635887183 2211 17 -0.4002656256150843 2211 18 -0.4174399901406929 2211 19 -0.20648749860674087 2211 20 -0.45154877472182164 2211 21 -0.059464147938520284 2211 22 -0.12115742511691963 2211 23 -0.5946278773372029 2211 24 -0.24023058380915074 2211 25 -0.6821989238561628 2212 1 -0.25526848314869 2212 2 -0.3181298777253261 2212 3 -0.21440454547369825 2212 4 -0.15122357114722718 2212 5 -0.12334401429398412 2212 6 -0.1867094400559779 2212 7 -0.3713358200614252 2212 8 -0.39392595040511935 2212 9 -0.22638554637826047 2212 10 -0.6614641652583231 2212 11 -0.2754576665243107 2212 12 -0.4485629880673869 2212 13 -0.3962530522696781 2212 14 -0.4714683801127535 2212 15 -0.3435658313861367 2212 16 -0.43520435202074736 2212 17 -0.6480577323709866 2212 18 -0.4208500281365133 2212 19 -0.627273495608911 2212 20 -0.7589843418546423 2212 21 -0.23446763379386937 2212 22 -0.4975978756161865 2212 23 -0.3568691714134715 2212 24 -0.5707648545206823 2212 25 -0.40295093376866475 2213 1 -0.14895636128134981 2213 2 -0.2355450670811372 2213 3 -0.13338190821907148 2213 4 -0.07871870942698678 2213 5 -0.07674302232164365 2213 6 -0.22927265876237612 2213 7 -0.27278381922595685 2213 8 -0.32378919057025257 2213 9 -0.30166933944017416 2213 10 -0.27637059011620124 2213 11 -0.05632421718630809 2213 12 0.10199665521742673 2213 13 0.09389676503685251 2213 14 -0.6179651795245249 2213 15 -0.21241272564619607 2213 16 -0.3745316033620796 2213 17 -0.2973280494095718 2213 18 -0.28983181582769646 2213 19 -0.37030152974480757 2213 20 -0.3738448420671858 2213 21 -0.06996839383857889 2213 22 -0.5098008179313993 2213 23 -0.5162623997999987 2213 24 -0.6951273844881456 2213 25 -0.4418044299007775 2214 1 -0.2775502178909335 2214 2 -0.09745145126324672 2214 3 -0.14399420187673329 2214 4 -0.06373239961485666 2214 5 -0.14356697966361764 2214 6 0.03062022785700691 2214 7 -0.14709778472933266 2214 8 -0.0870966854053421 2214 9 -0.11357842875460733 2214 10 0.04537747830224248 2214 11 0.04265150099551107 2214 12 -0.22411520038133403 2214 13 -0.30777188053235705 2214 14 -0.2491350327905124 2214 15 -0.08293124877786795 2214 16 -0.322268446939745 2214 17 -0.3804051483098263 2214 18 -0.2891120082572982 2214 19 -0.44565764285294307 2214 20 -0.5670908270818629 2214 21 -0.4591833660825273 2214 22 -0.8029600127706402 2214 23 -0.8659521640517198 2214 24 -0.5375152838664263 2214 25 -0.9051690303461655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 17.0 9 55.0 10 123.0 11 263.0 12 661.0 13 1477.0 14 3013.0 15 5353.0 16 7937.0 17 10198.0 18 12238.0 19 13837.0 20 15190.0 21 16840.0 22 18503.0 23 20201.0 24 22806.0 25 26045.0 26 29826.0 27 33406.0 28 39847.0 29 49457.0 30 63062.0 31 82518.0 32 110246.0 33 149147.0 34 204862.0 35 285679.0 36 409125.0 37 646221.0 38 1448504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.16670476249823 22.441229233854777 11.965484370815782 24.426581632831212 2 14.207632090835249 22.673069899875465 40.33752484463966 22.781773164649625 3 17.24908723330392 28.439923990743893 30.57660952716697 23.734379248785217 4 11.298625666569158 18.029477989463714 40.045945300402295 30.625951043564843 5 12.63332520991172 38.43692664120851 35.65036936518144 13.27937878369833 6 29.549865651565753 40.318942002054676 17.53370519348214 12.597487152897427 7 24.194387775505653 34.281479755791395 24.76599180156051 16.75814066714244 8 22.890240802853317 38.61039622256141 21.96260189789826 16.536761076687018 9 23.613147118181253 16.456599037689813 23.481608315441964 36.44864552868697 10 14.052467343273031 30.753300153114605 34.466006079328665 20.728226424283697 11 31.51153383599562 26.780178734114813 23.40999107752511 18.298296352364456 12 19.47564648602697 28.771177435351397 33.3437270568839 18.409449021737732 13 25.60975544308064 25.027491119389005 28.00077173703624 21.361981700494116 14 19.16760630124944 23.5591658513626 30.007384695968952 27.265843151419006 15 22.243761017053306 30.965416548932286 25.22298421970215 21.56783821431226 16 20.559955692133315 32.45081644328475 26.855096746355713 20.134131118226225 17 18.362677327159435 31.361888148010404 30.344810375626196 19.930624149203965 18 18.652080181482223 31.009814186804974 31.17103153881533 19.167074092897472 19 19.409742384837404 29.216877168841453 31.687518314064878 19.68586213225626 20 21.04369311749343 28.85633776814625 30.48329451832221 19.616674596038106 21 21.435695783857525 29.95052128545373 29.297468801093096 19.316314129595646 22 19.36285704011923 29.60844128417176 31.434316317916185 19.594385357792827 23 19.393461916816623 30.142664016928837 30.85695011455304 19.606923951701496 24 19.44313108420467 28.793922060999428 31.102451579941064 20.660495274854842 25 20.385810950058392 28.890176332692175 29.89270089132376 20.83131182592567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1844.0 1 1844.0 2 2225.0 3 2606.0 4 2606.0 5 2606.0 6 5521.5 7 8437.0 8 8437.0 9 8437.0 10 13925.5 11 19414.0 12 19414.0 13 19414.0 14 31290.5 15 43167.0 16 43167.0 17 43167.0 18 67873.5 19 92580.0 20 92580.0 21 92580.0 22 130927.0 23 169274.0 24 169274.0 25 169274.0 26 216484.5 27 263695.0 28 263695.0 29 263695.0 30 312897.5 31 362100.0 32 362100.0 33 362100.0 34 401293.0 35 440486.0 36 440486.0 37 440486.0 38 461519.0 39 482552.0 40 482552.0 41 482552.0 42 466153.5 43 449755.0 44 449755.0 45 449755.0 46 440136.0 47 430517.0 48 430517.0 49 430517.0 50 389754.5 51 348992.0 52 348992.0 53 348992.0 54 302258.5 55 255525.0 56 255525.0 57 255525.0 58 214177.0 59 172829.0 60 172829.0 61 172829.0 62 138439.5 63 104050.0 64 104050.0 65 104050.0 66 76934.5 67 49819.0 68 49819.0 69 49819.0 70 34836.5 71 19854.0 72 19854.0 73 19854.0 74 13238.5 75 6623.0 76 6623.0 77 6623.0 78 4260.5 79 1898.0 80 1898.0 81 1898.0 82 1202.0 83 506.0 84 506.0 85 506.0 86 301.0 87 96.0 88 96.0 89 96.0 90 64.0 91 32.0 92 32.0 93 32.0 94 19.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09638660258424503 2 0.0 3 0.13435626924813887 4 0.013121672790561197 5 0.021654785157429215 6 0.014141352884715236 7 0.022218292577882763 8 0.020071597642821626 9 5.366737337652841E-5 10 0.3865929241178224 11 0.06322016583755047 12 0.0013685180211014747 13 2.6833686688264206E-5 14 8.855116607127189E-4 15 0.0013416843344132103 16 1.8783580681784945E-4 17 5.366737337652841E-5 18 3.4883792694743467E-4 19 4.025053003239631E-4 20 2.1466949350611365E-4 21 1.6100212012958525E-4 22 0.0031395413425269122 23 2.6833686688264206E-5 24 2.6833686688264206E-5 25 0.0017978570081137017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3726659.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.3481191672788 #Duplication Level Percentage of deduplicated Percentage of total 1 89.47931042837494 70.10535676448252 2 8.203817350064122 12.855073187388264 3 1.2839224382718348 3.017787245857946 4 0.3623130926159629 1.1354619742456311 5 0.16261060011901046 0.6370117337993475 6 0.1014801426422108 0.4770466985306629 7 0.058584791961436414 0.32130057833894005 8 0.05313815575089257 0.3330619647280282 9 0.03560379898532194 0.2510541617138859 >10 0.22079714782775686 3.241886569213983 >50 0.020659650460355033 1.1149682531796812 >100 0.015251520130451552 2.289044151832751 >500 0.0013414305593589027 0.7412682301423887 >1k 8.94287004365734E-4 1.3939841451011794 >5k 1.7197827007033265E-4 0.9879533858559043 >10k+ 1.0318696204219958E-4 1.09774095558878 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16795 0.4506717679293974 No Hit GGTATCAACGCAGAGTACTTTTTTT 13682 0.3671385012688309 No Hit TATCAACGCAGAGTACTTTTTTTTT 10258 0.27525995804821424 No Hit CCCATGTACTCTGCGTTGATACCAC 9145 0.2453940647641762 No Hit GTACATGGGAAGCAGTGGTATCAAC 8688 0.23313106994763944 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6802 0.18252273685357312 No Hit ATTCCATTCCATTCCATTCCATTCC 6416 0.17216493379190315 No Hit GAATGGAATGGAATGGAATGGAATG 5610 0.1505369823211622 No Hit GAGTACATGGGAAGCAGTGGTATCA 4461 0.11970507631634664 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 5 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 6 0.0 2.6833686688264206E-5 0.0 2.6833686688264206E-5 0.0 7 0.0 2.6833686688264206E-5 0.0 8.050106006479262E-5 0.0 8 0.0 2.6833686688264206E-5 0.0 8.050106006479262E-5 0.0 9 0.0 2.6833686688264206E-5 0.0 8.050106006479262E-5 0.0 10 0.0 2.6833686688264206E-5 0.0 1.0733474675305682E-4 0.0 11 0.0 2.6833686688264206E-5 0.0 1.6100212012958525E-4 0.0 12 0.0 2.6833686688264206E-5 0.0 1.6100212012958525E-4 0.0 13 0.0 2.6833686688264206E-5 0.0 1.8783580681784945E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 40 2.719318E-4 16.659306 8 TACCGTC 50 8.567179E-5 15.231365 7 CGAACCG 60 4.1550488E-4 12.6446085 12 CGCCCTA 175 0.0 11.92222 16 ACCATCG 75 0.0026041386 10.154243 8 TGTGCGA 75 0.002605303 10.153696 10 GTCTCGC 150 2.4132532E-8 10.142769 1 TATACCG 95 1.611352E-4 10.020635 5 TATTCCG 235 0.0 9.722148 5 GACTGTC 265 0.0 9.699218 7 TAGACTG 540 0.0 9.695892 5 CTGTGCG 90 0.0010940084 9.51909 9 GTAGGAC 170 1.668559E-8 9.4995165 3 CCGCCCT 220 1.8189894E-11 9.483457 15 GTCCCGC 235 7.2759576E-12 9.281681 12 CCCGTAC 165 1.09570465E-7 9.196202 16 TCTCGCT 145 1.7091716E-6 9.171947 2 TAATACT 775 0.0 8.966851 4 GCGAACC 85 0.0075169094 8.9298 11 CCCCGTA 160 6.9196176E-7 8.89074 15 >>END_MODULE