FastQCFastQC Report
Wed 25 May 2016
SRR1294674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3253048
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC115840.35609680521160464No Hit
CCCATGTACTCTGCGTTGATACCAC107890.3316581864147101No Hit
GTATCAACGCAGAGTACTTTTTTTT79730.24509321719199964No Hit
GGTATCAACGCAGAGTACTTTTTTT54700.16814999348303497No Hit
GAGTACATGGGAAGCAGTGGTATCA53920.16575224220484913No Hit
TATCAACGCAGAGTACTTTTTTTTT46410.1426662010520595No Hit
CATGTACTCTGCGTTGATACCACTG41690.12815673177893472No Hit
GCGTTGATACCACTGCTTCCCATGT35400.10882101954843582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAA551.9538833E-413.82426213
TCGCGCG1658.0035534E-1110.9361269
ACAGCGT700.001495885410.8538998
CGCCCTA2900.010.48737116
ATCGCCG750.00265310410.13139719
TATCGCG1554.0870873E-89.8035217
AGCCGGC800.0044967529.50535118
AGCGTCG1201.6928134E-59.5041817
GCGCCTT1901.0440999E-99.5041813
CCGCCCT3300.09.5041815
ATCGCGC1701.6749254E-89.4971628
CGCGCGC1901.0586518E-99.49701610
TAACGCT800.00452941149.4970154
TTAGACA3450.09.3593784
CGACTAA3050.09.34837317
CTAATAC4500.09.2859723
GCGCGCC2054.2564352E-109.26509711
GTGTAAG4750.09.2007891
CGCCTTT2401.0913936E-119.10817214
TAGTACC2304.1836756E-119.0841024
Produced by FastQC (version 0.11.5)