##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3253048 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.93191001177972 31.0 30.0 33.0 25.0 34.0 2 30.16781861196023 31.0 30.0 34.0 25.0 34.0 3 30.108681765531895 31.0 30.0 34.0 25.0 34.0 4 33.75143619153483 35.0 35.0 37.0 28.0 37.0 5 34.084583135570085 35.0 35.0 37.0 28.0 37.0 6 34.14170894496485 37.0 35.0 37.0 29.0 37.0 7 34.052335840110565 37.0 35.0 37.0 29.0 37.0 8 33.895625579456556 37.0 35.0 37.0 28.0 37.0 9 35.04683269352312 39.0 35.0 39.0 27.0 39.0 10 35.15788177733621 38.0 35.0 39.0 27.0 39.0 11 35.38385723174082 39.0 35.0 39.0 28.0 39.0 12 35.17319080443941 39.0 35.0 39.0 27.0 39.0 13 35.03252180724047 38.0 35.0 39.0 27.0 39.0 14 35.92555658570055 39.0 35.0 41.0 27.0 41.0 15 35.91222047753369 39.0 35.0 41.0 27.0 41.0 16 36.02272299701695 39.0 35.0 41.0 27.0 41.0 17 36.01314828431674 39.0 35.0 41.0 27.0 41.0 18 35.897387311838 39.0 35.0 41.0 27.0 41.0 19 36.18415098701279 39.0 35.0 41.0 27.0 41.0 20 36.28915497096877 39.0 35.0 41.0 27.0 41.0 21 36.32964161610895 39.0 36.0 41.0 27.0 41.0 22 36.338396789718445 39.0 36.0 41.0 27.0 41.0 23 36.31188288644988 39.0 35.0 41.0 27.0 41.0 24 36.31333291116516 39.0 35.0 41.0 27.0 41.0 25 35.07416828771048 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0429066488338066 1101 2 0.984275504271892 1101 3 1.318206525842644 1101 4 1.1121529518595423 1101 5 0.8334576001175407 1101 6 0.7802893575679093 1101 7 0.7786847323959734 1101 8 0.9986066536876521 1101 9 1.2436816647472853 1101 10 1.2110202334870408 1101 11 0.9678174907729868 1101 12 1.1840644912803953 1101 13 1.2605684113483306 1101 14 1.254164223563535 1101 15 1.4614421776041837 1101 16 1.377272439278407 1101 17 1.2525717534352907 1101 18 1.3484735801666687 1101 19 1.1829515230430516 1101 20 1.1658227228863112 1101 21 1.1053191274237975 1101 22 1.0120621496873525 1101 23 1.0306369706043483 1101 24 1.0999973763777362 1101 25 1.1337278242294175 1102 1 0.8909471574687124 1102 2 0.8608123939540704 1102 3 1.201675483943884 1102 4 0.9855202716135878 1102 5 0.8254368150169569 1102 6 0.7096879171182664 1102 7 0.6694838972020989 1102 8 0.7045599315281663 1102 9 1.094981548555701 1102 10 1.0122968127578673 1102 11 0.7455812433791493 1102 12 0.9691272759968967 1102 13 0.9061027270888999 1102 14 1.144066660326736 1102 15 1.2992463654932678 1102 16 1.2272249387728351 1102 17 1.2218186271888811 1102 18 1.2639127471733573 1102 19 1.0029209902784046 1102 20 0.23342024664547978 1102 21 0.5047470838017745 1102 22 0.5849788131703448 1102 23 0.7648272487153491 1102 24 0.8681408630523819 1102 25 0.7891973609963401 1103 1 0.6339237983635542 1103 2 0.6809666484323316 1103 3 0.9589297238275876 1103 4 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-1.246140083426127 2207 10 -0.13373972252693989 2207 11 0.1658140167268627 2207 12 0.493770428148288 2207 13 0.7196737162651061 2207 14 0.9277876272935615 2207 15 0.9866281328459863 2207 16 0.8774434309200316 2207 17 0.802035005250886 2207 18 0.8679519018111819 2207 19 -0.30953312324088245 2207 20 0.3493287694994862 2207 21 -0.22908337986752514 2207 22 0.3319273637309408 2207 23 -0.4808539021490077 2207 24 -1.495410831367586 2207 25 -0.1459501048815781 2208 1 0.66081313729158 2208 2 0.2731635232028147 2208 3 0.7847186352983577 2208 4 0.6571869724037995 2208 5 0.5705734069265347 2208 6 0.46022728087928044 2208 7 0.5243293676565202 2208 8 0.6241197991914689 2208 9 0.03801828628228776 2208 10 0.14905074123680606 2208 11 0.3884480650386237 2208 12 0.4146849923905478 2208 13 0.7095563429562262 2208 14 1.0561740180132233 2208 15 0.9202516393308642 2208 16 0.7196497518601248 2208 17 0.6232977317608857 2208 18 0.9095998197669815 2208 19 -1.3451163777348114 2208 20 -3.801045409232984E-4 2208 21 -0.1310227200843883 2208 22 0.29621202636482735 2208 23 0.47694171241000305 2208 24 0.5785708863492474 2208 25 0.7242145465558352 2209 1 0.2399040831310124 2209 2 -1.3023967098602753 2209 3 -0.22036222805791894 2209 4 0.13905122521090618 2209 5 0.08817641295992473 2209 6 0.17104589317570884 2209 7 0.14126014325513836 2209 8 0.18427338969763696 2209 9 -2.2972100903321078 2209 10 -0.4222992311825422 2209 11 -0.04376977779368474 2209 12 0.2988623555061807 2209 13 0.42706865404993977 2209 14 0.6097290248949321 2209 15 0.7264307359565905 2209 16 0.6732921327809365 2209 17 0.38121747841968556 2209 18 0.6966463837170451 2209 19 0.4051691223773588 2209 20 0.3787275145096416 2209 21 0.40712898804372344 2209 22 0.2746444948783946 2209 23 0.46480242376294 2209 24 0.5186160574678169 2209 25 0.5150814058118343 2210 1 0.28114266500630336 2210 2 0.30139108102835976 2210 3 0.18953160781432743 2210 4 0.37810545161271136 2210 5 0.2720306036184894 2210 6 0.21052993900140393 2210 7 0.20618335704833868 2210 8 0.3577150518415593 2210 9 0.4782049544098612 2210 10 -1.344037992178123 2210 11 -0.30390531349265615 2210 12 -2.166307920754683 2210 13 -0.6044647599365831 2210 14 -0.3094760989497516 2210 15 -0.5475095190280399 2210 16 0.0707479204328223 2210 17 0.20065972450845493 2210 18 0.4312119353353836 2210 19 0.3472347285457005 2210 20 0.44978956101579826 2210 21 0.39356606671707084 2210 22 0.36627742424150256 2210 23 0.23628365677090102 2210 24 0.22710515849177426 2210 25 0.2751984118257269 2211 1 -0.37521062672053773 2211 2 -0.10331539453182259 2211 3 -1.4950707384643707 2211 4 -0.5845789203872087 2211 5 -0.5308712060876957 2211 6 -0.3927884418256795 2211 7 -0.4928485398237967 2211 8 -0.10260601617490295 2211 9 0.1820365211799455 2211 10 0.1412769333089514 2211 11 -0.18215084752487343 2211 12 -0.31065139542633347 2211 13 -0.34422580282803494 2211 14 -0.8204718954661772 2211 15 -0.42462583523727915 2211 16 -0.15604542516074105 2211 17 -0.17163622775209575 2211 18 -0.1735307313113239 2211 19 -0.1440081686763648 2211 20 -0.14739716172945094 2211 21 -0.2293870512183105 2211 22 -0.2745965005868598 2211 23 -0.5958659696285693 2211 24 -0.3654435899785611 2211 25 -0.18172750610005295 2212 1 -0.27373536943500554 2212 2 -0.18828067957615602 2212 3 -2.2922443948475113 2212 4 -1.2520256765296125 2212 5 -0.5387274078569391 2212 6 -0.392011642867061 2212 7 -0.21485738736624427 2212 8 -0.2829331462253535 2212 9 -0.7172114771984894 2212 10 -0.12875938083803362 2212 11 -0.15502432450902148 2212 12 0.1246821511325038 2212 13 -0.2488965734276647 2212 14 0.10633763229196802 2212 15 0.2143463388337068 2212 16 -1.0516415813102569 2212 17 -1.2697161978478775 2212 18 -0.20909637778606083 2212 19 -0.027653087657249387 2212 20 -0.1805191648909812 2212 21 -0.07245091752328392 2212 22 -0.2570925552568255 2212 23 0.03241165524984524 2212 24 -0.03576309919134957 2212 25 0.06887353975790944 2213 1 -3.022255338925113 2213 2 -1.3557059563112723 2213 3 -2.3567995546006983 2213 4 -1.0099554873737517 2213 5 -0.796269885227403 2213 6 -0.673890953824646 2213 7 -0.40074590596879744 2213 8 -0.18167387501811305 2213 9 -0.20710953150937428 2213 10 -0.15093307398397116 2213 11 -0.39523488650889504 2213 12 -0.25179915062290803 2213 13 -0.6363213033905097 2213 14 -0.19341539067519875 2213 15 -0.23468169144653928 2213 16 -1.5229473008215493 2213 17 -0.8999472911463613 2213 18 -0.21265083493688763 2213 19 -0.19231038446984172 2213 20 -0.252920342638447 2213 21 -0.042365265647717365 2213 22 0.037188862961080815 2213 23 0.044362839421886235 2213 24 0.10846684041764831 2213 25 -0.0779151300271792 2214 1 -1.24466719569811 2214 2 -0.9202812829789195 2214 3 -6.536546764280853 2214 4 -2.3487437042645922 2214 5 -1.6159456133802337 2214 6 -0.9914722438993095 2214 7 -0.892666604343411 2214 8 -0.6231533882329643 2214 9 -0.48923589346019014 2214 10 -0.6109405740669871 2214 11 -1.2772161850367851 2214 12 -0.9858250304378515 2214 13 -5.859481119851964 2214 14 -4.327072455309182 2214 15 -2.4552748104330533 2214 16 -3.2258496690622565 2214 17 -3.6614030318151407 2214 18 -1.5373166628938648 2214 19 -1.1797656438221935 2214 20 -0.7125666397581796 2214 21 -0.6446972998004838 2214 22 -0.6253857874483586 2214 23 -0.47182929640250393 2214 24 -0.32973402810834784 2214 25 -0.4114210500898423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1291.0 3 143.0 4 174.0 5 361.0 6 46.0 7 60.0 8 111.0 9 292.0 10 799.0 11 1914.0 12 3854.0 13 7104.0 14 11204.0 15 15444.0 16 19165.0 17 21817.0 18 23304.0 19 23574.0 20 23834.0 21 24437.0 22 24897.0 23 26059.0 24 27581.0 25 29864.0 26 33412.0 27 37707.0 28 44188.0 29 53592.0 30 66009.0 31 82451.0 32 106996.0 33 142188.0 34 191340.0 35 259741.0 36 363013.0 37 543235.0 38 1041847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.037072572413784 21.276456945603076 12.871715796946297 22.81475468503684 2 14.484064189215164 21.93597994663714 40.87711932827386 22.702836535873836 3 17.799942421684882 28.385894050978955 30.159472247990294 23.654691279345872 4 11.670594212820715 18.025099388139367 39.636168363477324 30.668138035562592 5 12.159303994594444 39.53711383992896 35.62995679798885 12.673625367487743 6 29.312379337542342 40.04037155116088 18.024464101803737 12.622785009493038 7 24.64471639447739 34.38167118025521 24.250920661325825 16.722691763941576 8 23.481669546772448 38.100754282170804 21.890885311091413 16.52669085996534 9 24.39636612298372 17.307826591974475 22.310974996408635 35.984832288633164 10 14.21109701234283 30.924966355212057 34.317622178644214 20.5463144538009 11 31.277035471276537 26.903467891787674 23.74741353032387 18.07208310661192 12 19.92618303089436 28.567763780777323 32.71133252579042 18.7947206625379 13 25.92980850152815 24.89126395881626 27.375797910299383 21.803129629356206 14 19.552756773583504 24.19231003482687 29.099662928053384 27.155270263536245 15 22.44743525381685 31.243724714289932 24.7180735611139 21.590766470779315 16 20.569029492074613 32.03140932553555 26.875471584360426 20.524089598029413 17 19.50439692541284 30.9796832146588 29.29113958315646 20.224780276771906 18 19.92060645707469 30.306753376688345 30.03110786162312 19.741532304613845 19 20.29461336686796 29.869209408604547 30.18676503928763 19.64941218523986 20 21.620731234658283 29.08112730471802 29.30515481676069 19.992986643863006 21 21.55785009317116 29.582289297528213 29.002038138348563 19.85782247095207 22 20.624384199706476 29.31321675462825 29.597388499196057 20.465010546469216 23 20.442863180845848 29.616044561938647 29.488231678769157 20.452860578446348 24 20.50514043676946 29.230869570300218 29.417302312073645 20.84668768085668 25 20.966643933612097 28.910108457280774 28.886883212085245 21.23636439702188 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3975.0 1 3975.0 2 4045.0 3 4115.0 4 4115.0 5 4115.0 6 6351.5 7 8588.0 8 8588.0 9 8588.0 10 13631.0 11 18674.0 12 18674.0 13 18674.0 14 30181.0 15 41688.0 16 41688.0 17 41688.0 18 62578.5 19 83469.0 20 83469.0 21 83469.0 22 114505.0 23 145541.0 24 145541.0 25 145541.0 26 184821.5 27 224102.0 28 224102.0 29 224102.0 30 263330.5 31 302559.0 32 302559.0 33 302559.0 34 330191.5 35 357824.0 36 357824.0 37 357824.0 38 370074.0 39 382324.0 40 382324.0 41 382324.0 42 376604.0 43 370884.0 44 370884.0 45 370884.0 46 376930.5 47 382977.0 48 382977.0 49 382977.0 50 350552.5 51 318128.0 52 318128.0 53 318128.0 54 276963.0 55 235798.0 56 235798.0 57 235798.0 58 200063.5 59 164329.0 60 164329.0 61 164329.0 62 135848.0 63 107367.0 64 107367.0 65 107367.0 66 82277.0 67 57187.0 68 57187.0 69 57187.0 70 42135.5 71 27084.0 72 27084.0 73 27084.0 74 19002.0 75 10920.0 76 10920.0 77 10920.0 78 7363.0 79 3806.0 80 3806.0 81 3806.0 82 2523.0 83 1240.0 84 1240.0 85 1240.0 86 783.5 87 327.0 88 327.0 89 327.0 90 218.0 91 109.0 92 109.0 93 109.0 94 65.0 95 21.0 96 21.0 97 21.0 98 16.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1010436980948329 2 0.0529657109271059 3 0.05607049142834659 4 0.05084462325794148 5 0.048539093182762755 6 0.06898145984934745 7 0.06529261172906148 8 0.06178820601478982 9 0.06747518020023067 10 0.06679889137817824 11 0.06132709999975408 12 0.06252597563884701 13 0.06320226446089944 14 0.06486224611502812 15 0.0676288822052426 16 0.06267967764385893 17 0.0657844581450996 18 0.14364989388413574 19 0.07005737388443084 20 0.06480076531302335 21 0.06277189884686607 22 0.06753666100223545 23 0.06769036300724736 24 0.07826506095206712 25 0.06993441228042131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3253048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.81619486624885 #Duplication Level Percentage of deduplicated Percentage of total 1 86.76399497658015 65.78115950518637 2 9.698998733409738 14.706823559593868 3 1.9935278903349334 4.534250970148058 4 0.6239731635528835 1.8922908383694068 5 0.27630572505543677 1.0474224346731584 6 0.14782786221414446 0.6724647604973136 7 0.09881978169417531 0.5244497877896036 8 0.06737347868203994 0.4086400630859682 9 0.050827406111324164 0.3468186473653907 >10 0.2438116191159354 3.340861402658271 >50 0.019417941156240397 1.0345583804823282 >100 0.012135501963855076 1.7862998719875938 >500 0.0015543145897861435 0.8430680346384988 >1k 0.0012270904623865532 1.8029859279351013 >5k 1.2270904623865533E-4 0.5840942544316758 >10k+ 8.180603082577021E-5 0.6938115611574135 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 11584 0.35609680521160464 No Hit CCCATGTACTCTGCGTTGATACCAC 10789 0.3316581864147101 No Hit GTATCAACGCAGAGTACTTTTTTTT 7973 0.24509321719199964 No Hit GGTATCAACGCAGAGTACTTTTTTT 5470 0.16814999348303497 No Hit GAGTACATGGGAAGCAGTGGTATCA 5392 0.16575224220484913 No Hit TATCAACGCAGAGTACTTTTTTTTT 4641 0.1426662010520595 No Hit CATGTACTCTGCGTTGATACCACTG 4169 0.12815673177893472 No Hit GCGTTGATACCACTGCTTCCCATGT 3540 0.10882101954843582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.0740401002383E-5 0.0 3 0.0 0.0 0.0 3.0740401002383E-5 0.0 4 0.0 0.0 0.0 6.1480802004766E-5 0.0 5 0.0 0.0 0.0 6.1480802004766E-5 0.0 6 0.0 0.0 0.0 6.1480802004766E-5 0.0 7 0.0 0.0 0.0 6.1480802004766E-5 0.0 8 0.0 0.0 0.0 6.1480802004766E-5 0.0 9 0.0 0.0 0.0 9.222120300714898E-5 0.0 10 3.0740401002383E-5 0.0 0.0 9.222120300714898E-5 0.0 11 3.0740401002383E-5 0.0 0.0 9.222120300714898E-5 0.0 12 3.0740401002383E-5 0.0 0.0 9.222120300714898E-5 0.0 13 3.0740401002383E-5 0.0 0.0 9.222120300714898E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAA 55 1.9538833E-4 13.824262 13 TCGCGCG 165 8.0035534E-11 10.936126 9 ACAGCGT 70 0.0014958854 10.853899 8 CGCCCTA 290 0.0 10.487371 16 ATCGCCG 75 0.002653104 10.131397 19 TATCGCG 155 4.0870873E-8 9.803521 7 AGCCGGC 80 0.004496752 9.505351 18 AGCGTCG 120 1.6928134E-5 9.50418 17 GCGCCTT 190 1.0440999E-9 9.50418 13 CCGCCCT 330 0.0 9.50418 15 ATCGCGC 170 1.6749254E-8 9.497162 8 CGCGCGC 190 1.0586518E-9 9.497016 10 TAACGCT 80 0.0045294114 9.497015 4 TTAGACA 345 0.0 9.359378 4 CGACTAA 305 0.0 9.348373 17 CTAATAC 450 0.0 9.285972 3 GCGCGCC 205 4.2564352E-10 9.265097 11 GTGTAAG 475 0.0 9.200789 1 CGCCTTT 240 1.0913936E-11 9.108172 14 TAGTACC 230 4.1836756E-11 9.084102 4 >>END_MODULE