FastQCFastQC Report
Wed 25 May 2016
SRR1294674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3253048
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT208230.6401073700726212No Hit
GGTATCAACGCAGAGTACTTTTTTT156140.4799806212512081No Hit
TATCAACGCAGAGTACTTTTTTTTT133150.40930843934672956No Hit
CCCATGTACTCTGCGTTGATACCAC123130.37850655754234186No Hit
GTACATGGGAAGCAGTGGTATCAAC115480.3549901507755188No Hit
ACGCAGAGTACTTTTTTTTTTTTTT85980.264305967818489No Hit
GAGTACATGGGAAGCAGTGGTATCA58140.17872469142785474No Hit
CATGTACTCTGCGTTGATACCACTG47610.14635504917234543No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40680.12505195127769403No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40350.12403751804461538No Hit
GCGTTGATACCACTGCTTCCCATGT37390.11493835934791002No Hit
ACGCAGAGTACATGGGAAGCAGTGG34560.10623882586423562No Hit
GTATCAACGCAGAGTACATGGGAAG33320.10242701613994015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT853.847499E-612.322246
AATCGCC802.9232237E-511.85313518
CCGTCCC855.2074945E-511.2013459
TACCGTC600.005778867311.1086867
ATCGCCG909.6995274E-510.53628319
ACACCGC750.0025991610.1565136
TACACCG951.6075771E-410.0228765
CGCCCTA2650.010.01925516
CGAACCG951.6756909E-49.98174112
GGTCTAA1356.3920015E-79.858261
TAGACAG4250.09.8577935
GTCCCGC2800.09.82132112
TTAGACT4100.09.7539684
ACTGTCG800.00443332559.5217318
GTATTAG5200.09.5061791
ATTGGAC2650.09.3182033
TTACACT5750.09.2733394
CTTAGAC3000.09.1808193
ACGGTAC1451.7140683E-69.1699023
TGTCCCG2900.09.16087511
Produced by FastQC (version 0.11.5)