##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294674_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3253048 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32868558963778 31.0 31.0 34.0 28.0 34.0 2 31.53483010395174 31.0 31.0 34.0 28.0 34.0 3 31.603765145795574 31.0 31.0 34.0 28.0 34.0 4 35.17565895123589 37.0 35.0 37.0 32.0 37.0 5 35.29600485452413 37.0 35.0 37.0 32.0 37.0 6 34.86012656437901 37.0 35.0 37.0 32.0 37.0 7 34.70964676820016 37.0 35.0 37.0 32.0 37.0 8 34.74935783302306 37.0 35.0 37.0 32.0 37.0 9 36.2372239819394 39.0 37.0 39.0 31.0 39.0 10 36.004508079806996 39.0 35.0 39.0 30.0 39.0 11 36.15189477683698 39.0 35.0 39.0 31.0 39.0 12 36.108204674508336 39.0 35.0 39.0 30.0 39.0 13 35.96213120740917 39.0 35.0 39.0 30.0 39.0 14 37.03936492790761 40.0 36.0 41.0 30.0 41.0 15 37.09259715811141 40.0 36.0 41.0 31.0 41.0 16 37.13394576409571 40.0 36.0 41.0 31.0 41.0 17 37.184115020743626 40.0 37.0 41.0 31.0 41.0 18 37.19019485725387 40.0 37.0 41.0 31.0 41.0 19 37.216151744456276 40.0 37.0 41.0 31.0 41.0 20 37.27144419633525 40.0 37.0 41.0 31.0 41.0 21 37.110500060251184 40.0 37.0 41.0 30.0 41.0 22 37.10707896102363 40.0 36.0 41.0 30.0 41.0 23 37.12218018301605 40.0 36.0 41.0 30.0 41.0 24 37.04718743775069 40.0 36.0 41.0 30.0 41.0 25 35.95176124053503 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4734635365028659 1101 2 0.44113372944180895 1101 3 0.36189143469243135 1101 4 0.3461159124941773 1101 5 0.3476266468316851 1101 6 0.463728848404692 1101 7 0.5106194079231017 1101 8 0.5220223162660105 1101 9 0.7437496714808063 1101 10 0.8148810988498738 1101 11 0.7740223609099388 1101 12 0.8428235549786507 1101 13 0.8190729492798283 1101 14 1.0276484213153765 1101 15 0.8935076804538724 1101 16 1.020990833381667 1101 17 0.9618655100784963 1101 18 0.9276380482957194 1101 19 1.0283498312002521 1101 20 1.0027726067538936 1101 21 1.1802123736522603 1101 22 1.1639833200072758 1101 23 1.1280635127713339 1101 24 1.293133671449418 1101 25 1.19884993781735 1102 1 0.39125964548557945 1102 2 0.4001910952802632 1102 3 0.31088322691070047 1102 4 0.286875981709926 1102 5 0.3552938142275437 1102 6 0.4026448282879045 1102 7 0.3762623559190885 1102 8 0.4742781700347507 1102 9 0.6001213358119273 1102 10 0.7414680869204986 1102 11 0.7293562474395046 1102 12 0.7272611158824489 1102 13 0.7711540317514078 1102 14 1.0375893474962865 1102 15 0.9180662313853674 1102 16 0.8756548227008025 1102 17 0.8219431816826699 1102 18 0.7836934331211296 1102 19 0.9254032716886087 1102 20 0.8169582188634905 1102 21 1.0899756759587191 1102 22 1.1055539286810472 1102 23 0.9829393568922455 1102 24 1.0145628941679519 1102 25 1.0506518242878329 1103 1 0.33834440836933766 1103 2 0.2925263788573069 1103 3 0.23787529162098053 1103 4 0.21044181851679866 1103 5 0.234328176541311 1103 6 0.29743007013960465 1103 7 0.36078122838225113 1103 8 0.423360668899349 1103 9 0.36450502722216527 1103 10 0.5192531934370805 1103 11 0.5435820793461588 1103 12 0.4831791702441066 1103 13 0.6607393293695338 1103 14 0.8141491943089747 1103 15 0.7470069380284841 1103 16 0.7390900409772527 1103 17 0.7476676466434995 1103 18 0.7503015480958766 1103 19 0.9123168053066806 1103 20 0.7596180133836796 1103 21 0.9553369582111841 1103 22 0.9073557118703235 1103 23 0.8947759553317596 1103 24 1.0440981366700512 1103 25 0.9471299792984098 1104 1 0.13970083728356641 1104 2 0.1640800625125003 1104 3 0.0854833391661316 1104 4 0.06774293513865359 1104 5 0.13563028578369796 1104 6 0.03545959640726437 1104 7 0.16056288236098482 1104 8 0.10168833964513624 1104 9 -0.11547845959759684 1104 10 0.2826950250735436 1104 11 0.38520947384306226 1104 12 0.4002177455973097 1104 13 -0.11793930363494809 1104 14 0.27069235138355197 1104 15 0.39651514487322004 1104 16 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0.25922523982789514 2207 4 0.2702025101634433 2207 5 0.17360887806015057 2207 6 0.3188357035284639 2207 7 0.30360186406345235 2207 8 0.23828271294610204 2207 9 0.39511558993083185 2207 10 0.5885548330245385 2207 11 0.4625557376138971 2207 12 0.45486938132469845 2207 13 0.5231729390965256 2207 14 0.4081171318230119 2207 15 0.44839870808140603 2207 16 0.5272601606020828 2207 17 0.6338440533381373 2207 18 0.5163397792380948 2207 19 0.6323921644522628 2207 20 0.5313802263582943 2207 21 0.8818513732266098 2207 22 0.8080834856508474 2207 23 0.6875166618673987 2207 24 0.8599891322294013 2207 25 0.8907104719354564 2208 1 0.25383546508235 2208 2 0.2283590910949087 2208 3 0.21930095048420384 2208 4 0.14097656797477498 2208 5 0.11178340495647632 2208 6 0.26904487420365086 2208 7 0.31396066904768816 2208 8 0.24878580685980722 2208 9 0.4201617988225408 2208 10 0.39522755122913367 2208 11 0.1278991849184763 2208 12 -0.04610210360147704 2208 13 0.16152697778779412 2208 14 -0.289604640916707 2208 15 0.25598727135973576 2208 16 0.38575949918779173 2208 17 0.28082485518690703 2208 18 0.43157967619139015 2208 19 0.5348931442124822 2208 20 0.4407089435492608 2208 21 0.738261476154932 2208 22 0.6816979228742568 2208 23 0.5989777611743392 2208 24 0.7581237455482892 2208 25 0.7160111276995664 2209 1 0.04367364675874086 2209 2 0.05114690467237537 2209 3 0.10921824975379835 2209 4 -0.490941157057172 2209 5 0.0033786751237627755 2209 6 0.19724940869870267 2209 7 0.17942184880791956 2209 8 0.21093431959448594 2209 9 0.2821589357481926 2209 10 0.317550816622564 2209 11 0.32595357233771693 2209 12 0.39632885974253185 2209 13 0.4644858887431127 2209 14 0.4200345249142998 2209 15 -0.08244405659625897 2209 16 -0.1177235414883171 2209 17 0.22125112827418292 2209 18 -0.09067552219176633 2209 19 0.27166860243664104 2209 20 0.36357282865251506 2209 21 0.6271369974647527 2209 22 0.6717289687140209 2209 23 0.6176273432962631 2209 24 0.6358283528168656 2209 25 0.7345617469845394 2210 1 -0.10059848123531623 2210 2 -0.14897912027608484 2210 3 -0.09286649567430416 2210 4 0.006299628522967282 2210 5 -0.026691828884828794 2210 6 -0.4041340025805269 2210 7 -0.1151963802229119 2210 8 -0.2735508385895642 2210 9 -0.025453162785396444 2210 10 0.2075146427520025 2210 11 0.07331915332646588 2210 12 0.07219622506491419 2210 13 0.18900264783307819 2210 14 0.21388504773426575 2210 15 0.042747829549043104 2210 16 0.16626689142169937 2210 17 -0.904786334939196 2210 18 -1.32982642621527 2210 19 -0.2970341014080802 2210 20 -0.1575500079201646 2210 21 -0.3898655413907264 2210 22 -0.30808808268317023 2210 23 -0.07852352736226464 2210 24 0.3196252688538408 2210 25 0.3946572216198234 2211 1 -0.2761811742402429 2211 2 -0.26957966090544616 2211 3 -0.23080404679023658 2211 4 -0.15309486909647063 2211 5 -0.16402143049144513 2211 6 -0.15289853641491646 2211 7 -0.2746749316046504 2211 8 -0.2107821790098825 2211 9 -0.2115854586826984 2211 10 -0.055492158708737804 2211 11 -0.5002591165752008 2211 12 -0.22299369413379821 2211 13 -0.28662432827099593 2211 14 -0.27148859727243746 2211 15 -0.379427317423918 2211 16 -0.39866502563346273 2211 17 -0.4929692051681229 2211 18 -0.5727117163561104 2211 19 -0.3060214420726268 2211 20 -0.5157190465203669 2211 21 -0.0865586127983633 2211 22 -0.20156128031993603 2211 23 -0.8213530508328759 2211 24 -0.3430804576708937 2211 25 -0.959771075037068 2212 1 -0.19850688541971095 2212 2 -0.2688706202754574 2212 3 -0.1504008663080043 2212 4 -0.033208431423467744 2212 5 -0.09124532649004635 2212 6 -0.14411804154746477 2212 7 -0.20449730949026446 2212 8 -0.3550488741066147 2212 9 -0.18815405477698732 2212 10 -0.4219929043399233 2212 11 -0.262874840771957 2212 12 -0.28394170527943174 2212 13 -0.21479104956024742 2212 14 -0.28526443934867984 2212 15 -0.10250797852829407 2212 16 -0.369436888816395 2212 17 -0.6058740868477059 2212 18 -0.3700450023059929 2212 19 -0.2954373226347542 2212 20 -0.5256020488934894 2212 21 -0.02031829185448686 2212 22 -0.3347494623618701 2212 23 -0.17559024973410686 2212 24 -0.3941854528075055 2212 25 -0.14982049860427793 2213 1 -0.23118440187072054 2213 2 -0.23960026052664674 2213 3 -0.12497180044108447 2213 4 -0.01294669153944028 2213 5 -0.09294059390269638 2213 6 -0.14844907934856622 2213 7 -0.22054633309168992 2213 8 -0.2087530101654309 2213 9 -0.25777495022592944 2213 10 -0.11746158385756189 2213 11 0.05229267658123149 2213 12 0.1340481958368045 2213 13 0.09761887654348556 2213 14 -0.6604094296844778 2213 15 -0.22888760798072383 2213 16 -0.2683963265157914 2213 17 -0.22642780312210675 2213 18 -0.08785534340987056 2213 19 -0.18323434983393838 2213 20 -0.25491040931483155 2213 21 -0.0430330161582404 2213 22 -0.34507651419314556 2213 23 -0.47109113413824133 2213 24 -0.6366522705655768 2213 25 -0.4447928729355084 2214 1 -0.2995959196520417 2214 2 -0.17888117982376883 2214 3 -0.16009754867032555 2214 4 -0.027472333930823822 2214 5 -0.03634537282275829 2214 6 -0.006815682889175889 2214 7 -0.06210869656236184 2214 8 -0.04480654324795097 2214 9 -0.14684854980430373 2214 10 0.01848593885078742 2214 11 -0.013088402248996545 2214 12 -0.22887150583327553 2214 13 -0.21215967477170494 2214 14 -0.31880059502658753 2214 15 -0.061445127665749055 2214 16 -0.37223376836211486 2214 17 -0.37263467602740263 2214 18 -0.29899734426295765 2214 19 -0.29929198876985197 2214 20 -0.38820562405484793 2214 21 -0.42071524467054644 2214 22 -0.7296479075222564 2214 23 -1.0540347636177572 2214 24 -0.4714313502074887 2214 25 -0.8489463188549067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 13.0 9 50.0 10 83.0 11 253.0 12 530.0 13 1311.0 14 2701.0 15 4651.0 16 7154.0 17 9255.0 18 10993.0 19 12423.0 20 13663.0 21 14913.0 22 16339.0 23 17997.0 24 19981.0 25 22762.0 26 26038.0 27 29365.0 28 35033.0 29 43658.0 30 55254.0 31 72984.0 32 97285.0 33 131793.0 34 180123.0 35 251504.0 36 359474.0 37 567993.0 38 1247472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39057747954101 21.669113909405656 11.931323763444963 25.008984847608367 2 14.317464728463891 22.23526981464768 39.73092312194594 23.716342334942492 3 17.484386466957922 27.854703022720894 30.610928734318744 24.049981776002443 4 11.521983814393403 18.08310964344996 39.41938909373085 30.975517448425787 5 12.334883709489414 38.836630611478725 35.76000459978065 13.068481079251207 6 29.57981705082386 40.05788799220781 17.457466407255 12.904828549713326 7 24.590420000528866 34.4817131854596 23.972906008608234 16.954960805403303 8 23.89799187649318 37.87967862644475 21.35117102103441 16.871158476027663 9 24.472732470654417 16.247362086906268 22.53082880808596 36.74907663435335 10 14.250301452186303 30.55448993240743 34.32953237644318 20.865676238963086 11 31.6955232089406 26.091344513801584 23.575569976563894 18.637562300693915 12 20.23388850240839 28.158043506261755 32.735506238389576 18.872561752940285 13 26.41606469257899 24.003557280297517 27.578236650131398 22.002141376992093 14 19.739552544607054 23.44276098157495 29.5142632208808 27.303423252937197 15 22.66430454956542 30.442383586421702 25.231585231419228 21.661726632593652 16 20.466670457986286 31.427539428443197 27.321017692654117 20.784772420916404 17 19.293818994929985 30.575549630853782 29.697757210393828 20.432874163822408 18 19.651537503615856 29.59259866100344 30.872788563780322 19.883075271600383 19 20.093500463567526 29.152341569342077 31.28684255181013 19.467315415280268 20 21.310176751483688 28.290596924355725 30.539292145628615 19.859934178531972 21 21.26257745053556 28.939633155899518 30.034361662492117 19.763427731072806 22 20.267350680427278 28.698082605482522 30.605393986269718 20.42917272782048 23 20.085138771648197 28.919761872118464 30.65712031999587 20.337979036237467 24 20.12537783806997 28.652706216663947 30.573182619248968 20.648733326017116 25 20.622525750029357 28.29942520856094 29.951989021846252 21.126060019563443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2010.0 1 2010.0 2 2378.5 3 2747.0 4 2747.0 5 2747.0 6 5821.0 7 8895.0 8 8895.0 9 8895.0 10 13307.0 11 17719.0 12 17719.0 13 17719.0 14 26788.5 15 35858.0 16 35858.0 17 35858.0 18 56128.5 19 76399.0 20 76399.0 21 76399.0 22 109818.0 23 143237.0 24 143237.0 25 143237.0 26 183946.0 27 224655.0 28 224655.0 29 224655.0 30 265042.5 31 305430.0 32 305430.0 33 305430.0 34 333307.5 35 361185.0 36 361185.0 37 361185.0 38 370494.0 39 379803.0 40 379803.0 41 379803.0 42 374805.5 43 369808.0 44 369808.0 45 369808.0 46 378319.5 47 386831.0 48 386831.0 49 386831.0 50 355253.0 51 323675.0 52 323675.0 53 323675.0 54 281213.5 55 238752.0 56 238752.0 57 238752.0 58 202420.0 59 166088.0 60 166088.0 61 166088.0 62 137621.5 63 109155.0 64 109155.0 65 109155.0 66 83356.5 67 57558.0 68 57558.0 69 57558.0 70 42365.0 71 27172.0 72 27172.0 73 27172.0 74 19048.5 75 10925.0 76 10925.0 77 10925.0 78 7252.0 79 3579.0 80 3579.0 81 3579.0 82 2378.0 83 1177.0 84 1177.0 85 1177.0 86 733.5 87 290.0 88 290.0 89 290.0 90 185.5 91 81.0 92 81.0 93 81.0 94 48.0 95 15.0 96 15.0 97 15.0 98 9.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09627893593946354 2 0.0 3 0.14091399819492365 4 0.012449862405965113 5 0.022102348320713373 6 0.017214624561334477 7 0.025022686415939757 8 0.023301223959806314 9 9.222120300714898E-5 10 0.3965204325297383 11 0.06397077448595902 12 0.0013833180451072347 13 3.074040100238299E-5 14 9.52952431073873E-4 15 0.0010451736340810218 16 9.222120300714898E-5 17 3.074040100238299E-5 18 4.611060150357449E-4 19 4.918464160381279E-4 20 1.8444240601429797E-4 21 1.2296160400953197E-4 22 0.002828116892219236 23 1.8444240601429797E-4 24 3.074040100238299E-5 25 0.0012910968421000859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3253048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.09011963430203 #Duplication Level Percentage of deduplicated Percentage of total 1 85.69268298486172 62.632884511478615 2 10.5161241444589 15.37249543615346 3 2.12683635952276 4.663521718803057 4 0.6823077050373243 1.994798071543364 5 0.28843368763783883 1.054082636800627 6 0.1660292348535371 0.7281057982941994 7 0.10038416211406312 0.513596329281423 8 0.07158895638603345 0.4185956309400013 9 0.048680259187835244 0.3202241371080938 >10 0.2696674464846632 3.5702882646442218 >50 0.02010926194733989 1.0332372481520773 >100 0.01381570813984432 1.9635218007576383 >500 0.0016066252680787024 0.8331238296751602 >1k 0.0014375068148085963 2.1813567467381945 >5k 8.455922440050567E-5 0.4453627822180631 >10k+ 2.1139806100126416E-4 2.2748050574117595 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 20823 0.6401073700726212 No Hit GGTATCAACGCAGAGTACTTTTTTT 15614 0.4799806212512081 No Hit TATCAACGCAGAGTACTTTTTTTTT 13315 0.40930843934672956 No Hit CCCATGTACTCTGCGTTGATACCAC 12313 0.37850655754234186 No Hit GTACATGGGAAGCAGTGGTATCAAC 11548 0.3549901507755188 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8598 0.264305967818489 No Hit GAGTACATGGGAAGCAGTGGTATCA 5814 0.17872469142785474 No Hit CATGTACTCTGCGTTGATACCACTG 4761 0.14635504917234543 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4068 0.12505195127769403 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4035 0.12403751804461538 No Hit GCGTTGATACCACTGCTTCCCATGT 3739 0.11493835934791002 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3456 0.10623882586423562 No Hit GTATCAACGCAGAGTACATGGGAAG 3332 0.10242701613994015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.222120300714898E-5 0.0 5 0.0 0.0 0.0 9.222120300714898E-5 0.0 6 0.0 0.0 0.0 9.222120300714898E-5 0.0 7 0.0 0.0 0.0 1.22961604009532E-4 0.0 8 0.0 0.0 3.0740401002383E-5 1.22961604009532E-4 0.0 9 0.0 0.0 3.0740401002383E-5 2.45923208019064E-4 0.0 10 0.0 0.0 3.0740401002383E-5 2.45923208019064E-4 0.0 11 0.0 0.0 3.0740401002383E-5 2.45923208019064E-4 0.0 12 0.0 0.0 3.0740401002383E-5 2.7666360902144695E-4 0.0 13 0.0 0.0 3.0740401002383E-5 3.0740401002382996E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCGT 85 3.847499E-6 12.32224 6 AATCGCC 80 2.9232237E-5 11.853135 18 CCGTCCC 85 5.2074945E-5 11.201345 9 TACCGTC 60 0.0057788673 11.108686 7 ATCGCCG 90 9.6995274E-5 10.536283 19 ACACCGC 75 0.00259916 10.156513 6 TACACCG 95 1.6075771E-4 10.022876 5 CGCCCTA 265 0.0 10.019255 16 CGAACCG 95 1.6756909E-4 9.981741 12 GGTCTAA 135 6.3920015E-7 9.85826 1 TAGACAG 425 0.0 9.857793 5 GTCCCGC 280 0.0 9.821321 12 TTAGACT 410 0.0 9.753968 4 ACTGTCG 80 0.0044333255 9.521731 8 GTATTAG 520 0.0 9.506179 1 ATTGGAC 265 0.0 9.318203 3 TTACACT 575 0.0 9.273339 4 CTTAGAC 300 0.0 9.180819 3 ACGGTAC 145 1.7140683E-6 9.169902 3 TGTCCCG 290 0.0 9.160875 11 >>END_MODULE