FastQCFastQC Report
Wed 25 May 2016
SRR1294673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991818
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC122300.3063766935266087No Hit
GTATCAACGCAGAGTACTTTTTTTT116030.2906695645943778No Hit
CCCATGTACTCTGCGTTGATACCAC115760.2899931810518416No Hit
GGTATCAACGCAGAGTACTTTTTTT70830.177437949325345No Hit
GTACTGGTTCACTATCGGTCAGTCA67870.1700227815997623No Hit
TATCAACGCAGAGTACTTTTTTTTT65740.1646868669864207No Hit
GAGTACATGGGAAGCAGTGGTATCA56440.14138921163239407No Hit
GGATACCACGTGTCCCGCCCTACTC52070.13044181874023314No Hit
GTTAATGATAGTGTGTCGAAACACA51710.12953997401685147No Hit
CATGTACTCTGCGTTGATACCACTG45550.11410840875009834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT701.0869677E-412.22000313
TAGACCG550.003077596412.0868645
GTCGTAC658.023772E-411.6930871
CTATACG855.3456602E-511.1727324
CCGTACA8450.011.02360217
CCCCGTA8500.010.62301715
TGTCCCG9500.010.39586911
CCCGTAC8900.010.14570616
GCGCTCA750.002657041410.1296899
TAGCACG951.6508531E-49.99665454
CGCCCTA10300.09.87404716
GTACTGG19500.09.5980761
CCCGCCC10700.09.50456714
GTATTAG5000.09.5006331
ATCGCCG2700.09.49789319
TCCCGCC10900.09.41725113
AGCGTAC10100.09.4065681
GCCCCGT8750.09.34163114
AATCGCC2750.09.33280918
GTCCCGC10700.09.23785112
Produced by FastQC (version 0.11.5)