##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294673_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3991818 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.939641286251028 31.0 30.0 34.0 25.0 34.0 2 30.102793263620736 31.0 30.0 34.0 25.0 34.0 3 29.98351252486962 31.0 30.0 34.0 25.0 34.0 4 33.50636176298619 35.0 33.0 37.0 27.0 37.0 5 33.9441728054736 35.0 35.0 37.0 28.0 37.0 6 34.038526305558015 37.0 35.0 37.0 29.0 37.0 7 33.9143751042758 37.0 35.0 37.0 28.0 37.0 8 33.73246325358521 37.0 35.0 37.0 27.0 37.0 9 34.80514016420588 38.0 35.0 39.0 27.0 39.0 10 34.925887152169764 38.0 35.0 39.0 27.0 39.0 11 35.17377921538507 39.0 35.0 39.0 27.0 39.0 12 34.92742579947282 38.0 35.0 39.0 27.0 39.0 13 34.794830575943095 38.0 35.0 39.0 27.0 39.0 14 35.662912236980745 39.0 34.0 41.0 25.0 41.0 15 35.66568365591818 39.0 34.0 41.0 26.0 41.0 16 35.77717646445805 39.0 34.0 41.0 27.0 41.0 17 35.77360140166711 39.0 34.0 41.0 27.0 41.0 18 35.66428479454725 39.0 34.0 41.0 26.0 41.0 19 35.9366784758223 39.0 34.0 41.0 27.0 41.0 20 36.051640630910526 39.0 35.0 41.0 27.0 41.0 21 36.08983500750786 39.0 35.0 41.0 27.0 41.0 22 36.098978710953254 39.0 35.0 41.0 27.0 41.0 23 36.07906046818768 39.0 35.0 41.0 27.0 41.0 24 36.070195835581686 39.0 35.0 41.0 27.0 41.0 25 34.83556489799886 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.074669253469601 1101 2 1.1125026709678068 1101 3 1.2984294536959524 1101 4 1.1195439624036325 1101 5 0.8048460839340947 1101 6 0.8252586833499436 1101 7 0.8216895386805589 1101 8 0.9850179918945372 1101 9 1.1811727332441393 1101 10 1.1880394775761332 1101 11 0.8922423563507493 1101 12 1.2278929450288913 1101 13 1.2360107086339625 1101 14 1.3269292296094761 1101 15 1.3944411473794318 1101 16 1.3601768727483048 1101 17 1.237139836960722 1101 18 1.34111012770758 1101 19 1.2449125827375624 1101 20 1.1545675177958046 1101 21 1.0279966081327174 1101 22 0.9877750188998675 1101 23 1.0189791071913632 1101 24 1.0852813956728298 1101 25 1.0662343598458008 1102 1 0.9394039811231316 1102 2 0.9665724464969045 1102 3 1.1835742222950287 1102 4 1.0216855734217773 1102 5 0.7981816798473744 1102 6 0.7038880399338439 1102 7 0.6734957948020082 1102 8 0.7993722524559246 1102 9 1.1061164952830396 1102 10 1.0059145440693413 1102 11 0.8368712730862029 1102 12 1.0348382775395066 1102 13 1.1129946792581222 1102 14 1.2258024884328336 1102 15 1.30206215366141 1102 16 1.2961637765743248 1102 17 1.2191052322156466 1102 18 1.2243765670434499 1102 19 1.0089380706734161 1102 20 0.23163314858512507 1102 21 0.5663657668102786 1102 22 0.712489728812173 1102 23 0.7623535194122795 1102 24 0.8957359696830594 1102 25 0.9136196935455843 1103 1 0.6728166738029415 1103 2 0.7049455548313652 1103 3 0.9538746895269838 1103 4 0.7215549697420442 1103 5 0.5243938679070084 1103 6 0.4601768653483873 1103 7 0.4318981423921855 1103 8 0.5576869162926457 1103 9 0.7030190103012117 1103 10 0.5855017913284897 1103 11 0.6441448713701945 1103 12 0.7521387135325597 1103 13 0.7381153764460038 1103 14 0.6249730552631902 1103 15 0.843924925006867 1103 16 0.8791486171131453 1103 17 0.9064007639693656 1103 18 0.9413816566574837 1103 19 0.7594187156663708 1103 20 -0.13978044402573886 1103 21 0.31032591277203636 1103 22 -0.36928188759163305 1103 23 0.2378042435327501 1103 24 0.4232353616880644 1103 25 0.4654836759579908 1104 1 0.4024290115792901 1104 2 0.3847180312699017 1104 3 0.637286324651626 1104 4 0.43127610939223615 1104 5 0.3486196947718554 1104 6 0.14497846367510903 1104 7 0.23503953355846363 1104 8 0.3411251985727972 1104 9 0.6060137545094904 1104 10 0.500388667100907 1104 11 0.3180084291206384 1104 12 0.4314331445672934 1104 13 0.35143929399092144 1104 14 0.650616708305229 1104 15 0.6694523159625803 1104 16 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0.26133052541509727 2207 8 0.33826280143882315 2207 9 -1.3507099871129071 2207 10 -0.2818711798860889 2207 11 0.09600832768968104 2207 12 0.4124041471364137 2207 13 0.5244316133197273 2207 14 0.7111322153766082 2207 15 0.7665506670382669 2207 16 0.7301303464418254 2207 17 0.7121678258731734 2207 18 0.700274052110494 2207 19 -0.43886369709780837 2207 20 0.12867426393399484 2207 21 -0.3599049152574736 2207 22 0.22660413244465616 2207 23 -0.5163131958203095 2207 24 -1.5033662488214645 2207 25 -0.2400220044067254 2208 1 0.5419439134363024 2208 2 0.23270911057091936 2208 3 0.710853035850576 2208 4 0.5459542312643464 2208 5 0.4412313866215243 2208 6 0.2588400265487323 2208 7 0.38079239927719755 2208 8 0.5622896708787053 2208 9 -0.1776347084439962 2208 10 0.0715806822626277 2208 11 0.25113385358288554 2208 12 0.29916877760684457 2208 13 0.5700924067273476 2208 14 0.8151571092532564 2208 15 0.7466296256863387 2208 16 0.5892739585275208 2208 17 0.5877652005543013 2208 18 0.8014627128542102 2208 19 -1.4795599661833805 2208 20 -0.13606891308283764 2208 21 -0.18907267634730118 2208 22 0.14678832638458061 2208 23 0.3813177793586746 2208 24 0.5295054104890937 2208 25 0.6370937508070682 2209 1 0.01788167885682057 2209 2 -1.551902331272995 2209 3 -0.38199556692530123 2209 4 -0.02365036841254664 2209 5 0.030897204460586636 2209 6 0.04970986374323161 2209 7 0.07564004935611024 2209 8 0.17516391721071045 2209 9 -2.076066970213418 2209 10 -0.4664540888453246 2209 11 -0.11416502206373735 2209 12 0.3432354259862933 2209 13 0.33742551574432156 2209 14 0.5966719285089255 2209 15 0.7375832629139438 2209 16 0.5792668638103322 2209 17 0.43046080454941205 2209 18 0.5791847879929364 2209 19 0.30859334809083094 2209 20 0.3446138893382198 2209 21 0.39435987682616513 2209 22 0.2836224661948066 2209 23 0.30676475042729834 2209 24 0.4093382455862624 2209 25 0.4711469013510623 2210 1 0.16300600960139633 2210 2 0.20979786066440909 2210 3 0.20984994030066773 2210 4 0.3344746449802116 2210 5 0.21397530492313166 2210 6 0.1190809396641157 2210 7 0.1793090843732017 2210 8 0.29467109420026105 2210 9 0.46785718543036836 2210 10 -1.4298030445160492 2210 11 -0.39954248365329903 2210 12 -2.155857049883778 2210 13 -0.5573472664909076 2210 14 -0.33913223411440896 2210 15 -0.5457476928853566 2210 16 -9.042574163586892E-4 2210 17 0.1509327195459349 2210 18 0.5198368853759163 2210 19 0.3800091849164886 2210 20 0.3900546565980463 2210 21 0.3739277268517327 2210 22 0.2950017828731504 2210 23 0.25190980551364106 2210 24 0.28788928654821433 2210 25 0.18381265782014822 2211 1 -0.38149489243564005 2211 2 -0.09570777707492795 2211 3 -1.4949747255632246 2211 4 -0.6423820816742776 2211 5 -0.5043055709058208 2211 6 -0.44983291933076686 2211 7 -0.4547825783632149 2211 8 -0.11342905147066773 2211 9 0.09129814889421084 2211 10 0.1109396053310121 2211 11 -0.2920899861965296 2211 12 -0.3170367928903488 2211 13 -0.32775253043634933 2211 14 -0.8494352703350998 2211 15 -0.4721745493325855 2211 16 -0.1805878314509286 2211 17 -0.2688144601715763 2211 18 -0.07151346133898073 2211 19 -0.12778495384896615 2211 20 -0.11904921131391433 2211 21 -0.23732782481561543 2211 22 -0.26663149660775076 2211 23 -0.6168295516517688 2211 24 -0.38255450650701306 2211 25 -0.2131596329307115 2212 1 -0.40695036118200534 2212 2 -0.36178119362269356 2212 3 -2.37224036709587 2212 4 -1.3938062931602033 2212 5 -0.6262421739725355 2212 6 -0.45724452588348186 2212 7 -0.3524674764396494 2212 8 -0.3276986120357037 2212 9 -0.991524336381282 2212 10 -0.2840149522386355 2212 11 -0.3232649900820519 2212 12 0.039090599444861596 2212 13 -0.43467265120008136 2212 14 -0.004997178865380647 2212 15 0.133794162497054 2212 16 -1.0978912547687898 2212 17 -1.4398438069853157 2212 18 -0.2503554051315575 2212 19 -0.10524282712392363 2212 20 -0.21863567442035503 2212 21 -0.12524377469689085 2212 22 -0.37808894677854 2212 23 -0.03748881384747449 2212 24 -0.17319908040811072 2212 25 0.09931479959319489 2213 1 -3.1961818377560647 2213 2 -1.4163646857834777 2213 3 -2.3899951777990687 2213 4 -1.1026457632025455 2213 5 -0.8106226962132936 2213 6 -0.5727573284636946 2213 7 -0.3876475726374409 2213 8 -0.11176734430025448 2213 9 -0.2810529303411684 2213 10 -0.24771901290800002 2213 11 -0.5088289802359682 2213 12 -0.21628179367381506 2213 13 -0.6325176817191078 2213 14 -0.21661274206109482 2213 15 -0.276375189616779 2213 16 -1.6264970849635105 2213 17 -1.1136797623547423 2213 18 -0.357412797775595 2213 19 -0.2313171198994084 2213 20 -0.25490544083703526 2213 21 -0.017769979213618114 2213 22 -0.11049981507421336 2213 23 0.026332226729344654 2213 24 0.08080889541058411 2213 25 0.01077822073631296 2214 1 -0.9263330323677579 2214 2 -0.7430366019991546 2214 3 -6.492991451542149 2214 4 -2.1075465184970135 2214 5 -1.2602132503908692 2214 6 -0.780016730015106 2214 7 -0.6962882108783717 2214 8 -0.41488530226369136 2214 9 -0.27030689451068923 2214 10 -0.06536097195066048 2214 11 -0.7258379126123273 2214 12 -0.5133995496897654 2214 13 -5.372215848937056 2214 14 -3.9318243689164234 2214 15 -2.2058577181730783 2214 16 -2.997119791678756 2214 17 -3.4915683714848313 2214 18 -1.1990328319438035 2214 19 -0.7456377484555503 2214 20 -0.3720430604515599 2214 21 -0.41346728978334113 2214 22 -0.3965421491311858 2214 23 -0.42165089056902616 2214 24 -0.2738091333973358 2214 25 -0.04839850123248368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1503.0 3 196.0 4 187.0 5 424.0 6 61.0 7 87.0 8 241.0 9 646.0 10 1574.0 11 3469.0 12 6812.0 13 11522.0 14 17447.0 15 23710.0 16 28712.0 17 31477.0 18 32389.0 19 32415.0 20 31724.0 21 31688.0 22 31672.0 23 32794.0 24 35113.0 25 37483.0 26 41327.0 27 46819.0 28 53881.0 29 65859.0 30 81102.0 31 100952.0 32 131332.0 33 172557.0 34 231443.0 35 314071.0 36 437998.0 37 653389.0 38 1267742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.962678898401606 20.179303378889728 12.188737970419355 23.669279752289313 2 15.500734762052643 21.287621935034654 39.81016746258579 23.401475840326917 3 18.656959774213234 27.49517809407197 29.20522207085664 24.642640060858156 4 12.276125603508753 17.953136917128283 38.21110518766262 31.559632291700346 5 12.318278653600341 39.455700644126416 35.19952881024587 13.02649189202737 6 29.742325942604346 38.94555457039242 18.14154112103544 13.170578365967803 7 26.087630056006773 33.903987602958786 22.569428168425897 17.438954172608547 8 24.879601047263467 36.77391216071408 21.017434755962295 17.32905203606015 9 25.52124577081204 17.011180823620332 21.078069738077996 36.389503667489635 10 15.064559964725083 30.3063718701095 33.346451965626514 21.28261619953891 11 32.463654411101196 25.66810712173015 23.307398457944394 18.56084000922426 12 21.295775015052172 27.71115775626664 31.00887388712953 19.98419334155166 13 27.57727636758773 23.617522869894458 26.321854206694468 22.483346555823346 14 20.718774996283848 23.98610498019348 27.894818770910394 27.400301252612273 15 23.44238933028513 30.50030895647912 24.151221976143294 21.906079737092448 16 21.42076152079566 30.334172382175133 26.27911500316586 21.965951093863346 17 20.760111588247486 29.763249725704377 27.56001101945224 21.916627666595893 18 21.31367561248501 28.657048532193407 28.787974572401925 21.241301282919657 19 21.52312169882505 29.242512740937197 28.869873229868315 20.36449233036943 20 22.73629443876546 27.806147643510272 28.45141308167764 21.00614483604663 21 22.646067232662308 28.11226165141386 28.306951118330186 20.93471999759364 22 22.238165140558383 28.211545480433326 27.74356066531102 21.806728713697275 23 22.12860402686919 28.154970366632547 28.14892913535441 21.567496471143848 24 22.117703865324 28.251921079489854 27.760692323178084 21.869682732008062 25 22.063520872053292 28.028705120986764 28.115466241375902 21.792307765584038 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4988.0 1 4988.0 2 4900.0 3 4812.0 4 4812.0 5 4812.0 6 7592.5 7 10373.0 8 10373.0 9 10373.0 10 15393.0 11 20413.0 12 20413.0 13 20413.0 14 32488.0 15 44563.0 16 44563.0 17 44563.0 18 66320.0 19 88077.0 20 88077.0 21 88077.0 22 119558.5 23 151040.0 24 151040.0 25 151040.0 26 194039.5 27 237039.0 28 237039.0 29 237039.0 30 275251.0 31 313463.0 32 313463.0 33 313463.0 34 346208.0 35 378953.0 36 378953.0 37 378953.0 38 389310.0 39 399667.0 40 399667.0 41 399667.0 42 413602.0 43 427537.0 44 427537.0 45 427537.0 46 462777.5 47 498018.0 48 498018.0 49 498018.0 50 471503.5 51 444989.0 52 444989.0 53 444989.0 54 397299.5 55 349610.0 56 349610.0 57 349610.0 58 302540.5 59 255471.0 60 255471.0 61 255471.0 62 219836.5 63 184202.0 64 184202.0 65 184202.0 66 141521.5 67 98841.0 68 98841.0 69 98841.0 70 73920.5 71 49000.0 72 49000.0 73 49000.0 74 34592.0 75 20184.0 76 20184.0 77 20184.0 78 13746.5 79 7309.0 80 7309.0 81 7309.0 82 4821.0 83 2333.0 84 2333.0 85 2333.0 86 1507.5 87 682.0 88 682.0 89 682.0 90 437.0 91 192.0 92 192.0 93 192.0 94 115.5 95 39.0 96 39.0 97 39.0 98 31.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09870189472566133 2 0.05238214768308575 3 0.055438399245656994 4 0.05072876569021935 5 0.04804828276239047 6 0.06590981853381091 7 0.062102029701754935 8 0.05944659801624222 9 0.0645570514487384 10 0.06465725641800303 11 0.0585698045351767 12 0.05912093186613217 13 0.060198135285726956 14 0.062001824732490315 15 0.06470735890263533 16 0.059621956712455335 17 0.06285356697123967 18 0.1402368544858508 19 0.06783876419215505 20 0.06142564615921867 21 0.05982236665098459 22 0.06478251262958382 23 0.0642564365409445 24 0.0762810328527002 25 0.06773855922289042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3991818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.76577107171443 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27036896352206 44.82087042065531 2 14.113922874652285 16.588311211513 3 4.720294205817115 8.321751860705662 4 1.9704605638242445 4.631825375981475 5 0.9885252110673524 2.9045723126101106 6 0.5464361722523309 1.9267045802330633 7 0.3363143483737688 1.3834640403265996 8 0.20788540907742478 0.9773237087194918 9 0.14657808220641835 0.7752396620765988 >10 0.6344033968223193 6.330235886321993 >50 0.03433923786685895 1.376785506059786 >100 0.023550896557644033 2.872744464631314 >500 0.0032007951159652016 1.3379302388047207 >1k 0.0033305569652390906 3.9252832607065096 >5k 2.5952391936927803E-4 0.9269124330418652 >10k+ 1.2976195968463901E-4 0.9000450376125543 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 12230 0.3063766935266087 No Hit GTATCAACGCAGAGTACTTTTTTTT 11603 0.2906695645943778 No Hit CCCATGTACTCTGCGTTGATACCAC 11576 0.2899931810518416 No Hit GGTATCAACGCAGAGTACTTTTTTT 7083 0.177437949325345 No Hit GTACTGGTTCACTATCGGTCAGTCA 6787 0.1700227815997623 No Hit TATCAACGCAGAGTACTTTTTTTTT 6574 0.1646868669864207 No Hit GAGTACATGGGAAGCAGTGGTATCA 5644 0.14138921163239407 No Hit GGATACCACGTGTCCCGCCCTACTC 5207 0.13044181874023314 No Hit GTTAATGATAGTGTGTCGAAACACA 5171 0.12953997401685147 No Hit CATGTACTCTGCGTTGATACCACTG 4555 0.11410840875009834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.01024846323154E-5 0.0 5 0.0 0.0 0.0 5.01024846323154E-5 0.0 6 0.0 0.0 0.0 7.51537269484731E-5 0.0 7 0.0 0.0 0.0 7.51537269484731E-5 0.0 8 0.0 0.0 0.0 7.51537269484731E-5 0.0 9 0.0 0.0 0.0 2.50512423161577E-4 0.0 10 0.0 0.0 0.0 3.006149077938924E-4 0.0 11 0.0 2.50512423161577E-5 0.0 3.006149077938924E-4 0.0 12 0.0 2.50512423161577E-5 0.0 3.256661501100501E-4 0.0 13 0.0 2.50512423161577E-5 0.0 3.256661501100501E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAAAT 70 1.0869677E-4 12.220003 13 TAGACCG 55 0.0030775964 12.086864 5 GTCGTAC 65 8.023772E-4 11.693087 1 CTATACG 85 5.3456602E-5 11.172732 4 CCGTACA 845 0.0 11.023602 17 CCCCGTA 850 0.0 10.623017 15 TGTCCCG 950 0.0 10.395869 11 CCCGTAC 890 0.0 10.145706 16 GCGCTCA 75 0.0026570414 10.129689 9 TAGCACG 95 1.6508531E-4 9.9966545 4 CGCCCTA 1030 0.0 9.874047 16 GTACTGG 1950 0.0 9.598076 1 CCCGCCC 1070 0.0 9.504567 14 GTATTAG 500 0.0 9.500633 1 ATCGCCG 270 0.0 9.497893 19 TCCCGCC 1090 0.0 9.417251 13 AGCGTAC 1010 0.0 9.406568 1 GCCCCGT 875 0.0 9.341631 14 AATCGCC 275 0.0 9.332809 18 GTCCCGC 1070 0.0 9.237851 12 >>END_MODULE