FastQCFastQC Report
Wed 25 May 2016
SRR1294673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991818
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT315530.790441848801724No Hit
GGTATCAACGCAGAGTACTTTTTTT213350.5344682548152245No Hit
TATCAACGCAGAGTACTTTTTTTTT188290.4716898415709333No Hit
CCCATGTACTCTGCGTTGATACCAC132480.3318788582044572No Hit
ACGCAGAGTACTTTTTTTTTTTTTT123210.30865635657737905No Hit
GTACATGGGAAGCAGTGGTATCAAC122710.3074037944615712No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63500.1590753887076014No Hit
GTACTGGTTCACTATCGGTCAGTCA63350.15869962007285904No Hit
GAGTACTTTTTTTTTTTTTTTTTTT62640.15692098186841183No Hit
GAGTACATGGGAAGCAGTGGTATCA58220.14584833276467013No Hit
GGATACCACGTGTCCCGCCCTACTC55280.13848326752371976No Hit
CATGTACTCTGCGTTGATACCACTG52100.1305169724671816No Hit
GTTAATGATAGTGTGTCGAAACACA51630.1293395640783222No Hit
AGCGTACACGGTGGATGCCCTGGCA44590.11170348948774719No Hit
GCGTTGATACCACTGCTTCCCATGT39950.10007971305305001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC905.536822E-712.64288217
GTACGAC550.00307188412.0898643
GTGCGAC550.003101162112.07455811
ATCGCCG2550.010.7837719
AAACCGT909.305547E-510.5796016
TGTCCCG11750.010.41568311
AATCGCC2650.010.37670518
CCGCACA750.002600941610.155789
TACGACA856.461167E-410.0817394
GGAGCGT951.6079385E-410.022788
TAGACAG5050.09.8044635
GTACTGG19000.09.7092741
GAAATCG3050.09.63760716
CGCCCTA12900.09.55566716
TAGCGCT800.0044340439.5216434
TGGACCG1304.121748E-69.5216425
TACTGGT17800.09.4990592
AAATCGC3000.09.482161517
CCCGCCC13050.09.44547714
GTCTTAG3450.09.371271
Produced by FastQC (version 0.11.5)