##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3991818 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.233364597283742 31.0 31.0 34.0 28.0 34.0 2 31.419238552459056 31.0 31.0 34.0 28.0 34.0 3 31.495535367594414 31.0 31.0 34.0 28.0 34.0 4 35.06805696051273 37.0 35.0 37.0 32.0 37.0 5 35.20067297657358 37.0 35.0 37.0 32.0 37.0 6 34.72459390683643 37.0 35.0 37.0 32.0 37.0 7 34.56069765705751 37.0 35.0 37.0 31.0 37.0 8 34.593074884676604 37.0 35.0 37.0 31.0 37.0 9 36.04193577963725 39.0 35.0 39.0 30.0 39.0 10 35.805636178803745 39.0 35.0 39.0 30.0 39.0 11 35.95256572318678 39.0 35.0 39.0 30.0 39.0 12 35.8963299428982 39.0 35.0 39.0 30.0 39.0 13 35.751453598335395 39.0 35.0 39.0 30.0 39.0 14 36.78371559023984 39.0 36.0 41.0 30.0 41.0 15 36.83654465208584 39.0 36.0 41.0 30.0 41.0 16 36.87588938173033 39.0 36.0 41.0 30.0 41.0 17 36.93653618476594 40.0 36.0 41.0 30.0 41.0 18 36.9417706418479 40.0 36.0 41.0 30.0 41.0 19 36.940212204063414 40.0 36.0 41.0 30.0 41.0 20 37.02042678298459 40.0 36.0 41.0 30.0 41.0 21 36.84810580041475 40.0 36.0 41.0 29.0 41.0 22 36.84291117480807 40.0 36.0 41.0 30.0 41.0 23 36.852050118517425 39.0 36.0 41.0 30.0 41.0 24 36.77018641631457 39.0 36.0 41.0 29.0 41.0 25 35.62030182738792 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5227442558609994 1101 2 0.5124266149445695 1101 3 0.4490486765534989 1101 4 0.4040264794055446 1101 5 0.43156305036691833 1101 6 0.4934084760191979 1101 7 0.48954972900518356 1101 8 0.5506379167403921 1101 9 0.728666706969797 1101 10 0.8213498231028566 1101 11 0.7532571147770781 1101 12 0.7565163692055634 1101 13 0.8077082520389851 1101 14 0.9864316384047029 1101 15 0.9014171943635034 1101 16 1.043909963297473 1101 17 1.0834832294652017 1101 18 1.0407701262986535 1101 19 1.2423218258772977 1101 20 1.078433797504026 1101 21 1.3337241017411188 1101 22 1.2926261930249652 1101 23 1.2246865926550186 1101 24 1.327625435168244 1101 25 1.3498634684380733 1102 1 0.4134603533233445 1102 2 0.41291696687151713 1102 3 0.37711896284501734 1102 4 0.3158813952393018 1102 5 0.31829438269588195 1102 6 0.4457838349416221 1102 7 0.5104246746718957 1102 8 0.5131763205196336 1102 9 0.6705180143734069 1102 10 0.7798419484245684 1102 11 0.6908945140053717 1102 12 0.7521798707756275 1102 13 0.7996426787990814 1102 14 0.9545910290100394 1102 15 0.8618173782670695 1102 16 0.8969841248556847 1102 17 0.8103668886406581 1102 18 0.8248188467424171 1102 19 1.0094009785062568 1102 20 0.8832432739379996 1102 21 1.1485321935343435 1102 22 1.1672474555973054 1102 23 1.0103198039587369 1102 24 1.0931073506055142 1102 25 1.1106816447434724 1103 1 0.3093313131999693 1103 2 0.29002242198071926 1103 3 0.2523229085781189 1103 4 0.22683331623942848 1103 5 0.2592892316781601 1103 6 0.3569122870754313 1103 7 0.3842355801283901 1103 8 0.4453729337033252 1103 9 0.24488234967800793 1103 10 0.5968993027830294 1103 11 0.5667211790859241 1103 12 0.5445251041620125 1103 13 0.7826387617628043 1103 14 0.875661723185921 1103 15 0.7704507460527026 1103 16 0.7214088658166986 1103 17 0.7039414042116405 1103 18 0.7016371724678692 1103 19 0.921224742396717 1103 20 0.755044900276495 1103 21 1.0509695351412418 1103 22 0.9053291917857464 1103 23 0.9310811703322699 1103 24 1.0110888125987287 1103 25 0.9246445753536392 1104 1 0.21457643862609643 1104 2 0.18774036751175416 1104 3 0.12334570293047875 1104 4 0.16964220605047586 1104 5 0.17759880777247616 1104 6 0.11620568386711483 1104 7 0.21562359324076397 1104 8 0.17032521366860465 1104 9 -0.04766480177964638 1104 10 0.33443421844098253 1104 11 0.41325625159699797 1104 12 0.43722215715155244 1104 13 -0.06821823974677699 1104 14 0.31342562528232065 1104 15 0.4209567278763231 1104 16 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0.15432871789476366 2207 6 0.28447788812660235 2207 7 0.2895891252339027 2207 8 0.31072549287865314 2207 9 0.4316242835831403 2207 10 0.5323247007681289 2207 11 0.37472041471916384 2207 12 0.35963260195632074 2207 13 0.5181384397018647 2207 14 0.40972924271267885 2207 15 0.48340008090448094 2207 16 0.5138457248509738 2207 17 0.5388136198539399 2207 18 0.5272647012099583 2207 19 0.6956815966845085 2207 20 0.5551262564464992 2207 21 0.8324773139256649 2207 22 0.7333596375946385 2207 23 0.567926648069033 2207 24 0.7276618980010952 2207 25 0.8215357109354784 2208 1 0.20843280109223983 2208 2 0.17060447332549344 2208 3 0.1672824512634037 2208 4 0.16542215777858615 2208 5 0.13534513151681438 2208 6 0.26422806296283596 2208 7 0.24676588390828869 2208 8 0.15190031482728017 2208 9 0.30186715407144504 2208 10 0.4063663829595896 2208 11 -0.006846499643494042 2208 12 -0.1202094940959384 2208 13 0.061186069225406925 2208 14 -0.42822929807770294 2208 15 0.17909701272223089 2208 16 0.18185200412916203 2208 17 0.1597033673274737 2208 18 0.32412817356524215 2208 19 0.45260064860039506 2208 20 0.38802100213317914 2208 21 0.7081923293297976 2208 22 0.6828307938389528 2208 23 0.6177067578052728 2208 24 0.7114090765028465 2208 25 0.7360301174710813 2209 1 -0.07392348425992878 2209 2 -0.0844830139401509 2209 3 -0.04185212534206073 2209 4 -0.5937315427031251 2209 5 -0.14189616579846387 2209 6 -0.0457474357849037 2209 7 0.027421783907747965 2209 8 0.07475068156898601 2209 9 0.10138895614515775 2209 10 0.11832064728870506 2209 11 0.2219378170684152 2209 12 0.1630571530401852 2209 13 0.2411887579134131 2209 14 0.22783373222909375 2209 15 -0.33393958063235374 2209 16 -0.3904273272423069 2209 17 0.036135151971336654 2209 18 -0.25698544059870443 2209 19 0.20427844431139164 2209 20 0.18071425158358778 2209 21 0.4634612175573025 2209 22 0.44138216604117986 2209 23 0.4175373704609129 2209 24 0.467697241690864 2209 25 0.47381109508114605 2210 1 -0.14318383648190292 2210 2 -0.16971500718101495 2210 3 -0.13617184958892636 2210 4 -0.05960234770758177 2210 5 -0.04644321495219117 2210 6 -0.4923438376473399 2210 7 -0.17012680503090394 2210 8 -0.29408186758627863 2210 9 -0.017442521589188686 2210 10 0.14278375826648215 2210 11 0.12156303403114777 2210 12 0.11833267658236224 2210 13 0.19833482931902324 2210 14 0.0660808569945317 2210 15 0.12639586595118857 2210 16 0.17756032998870808 2210 17 -0.8540815505624195 2210 18 -1.469732869953802 2210 19 -0.40233660027502083 2210 20 -0.21161060936127996 2210 21 -0.42816278104678673 2210 22 -0.3053479797639014 2210 23 -0.17594260468212042 2210 24 0.1462221093703775 2210 25 0.24959404357029058 2211 1 -0.2663345600863387 2211 2 -0.2537821057261809 2211 3 -0.16839155078613643 2211 4 -0.15757581134833742 2211 5 -0.14584364021661145 2211 6 -0.08234955861734505 2211 7 -0.2376299812021827 2211 8 -0.13645275535260026 2211 9 -0.15040785462078787 2211 10 -0.027930495199676386 2211 11 -0.32216162512028745 2211 12 -0.19902766371457403 2211 13 -0.182271286448767 2211 14 -0.21970628750677434 2211 15 -0.3062726309319146 2211 16 -0.2766594144093233 2211 17 -0.4593714164960687 2211 18 -0.48288512002932293 2211 19 -0.21921339584864796 2211 20 -0.37391763964914304 2211 21 0.0405652202035256 2211 22 -0.09245570071242781 2211 23 -0.7551436135470198 2211 24 -0.20759332426325727 2211 25 -0.7985573911524071 2212 1 -0.24741953143572104 2212 2 -0.3659903052035389 2212 3 -0.18277712548232827 2212 4 -0.10336476325893074 2212 5 -0.14092740124539205 2212 6 -0.18786451119996883 2212 7 -0.27789110358965985 2212 8 -0.46382529176838716 2212 9 -0.24924804049395988 2212 10 -0.5429050929190069 2212 11 -0.32187787864548056 2212 12 -0.4452165653077671 2212 13 -0.37109445394562357 2212 14 -0.3994853608376161 2212 15 -0.2568970449896071 2212 16 -0.36226560090454996 2212 17 -0.6074070722707816 2212 18 -0.40519205351369436 2212 19 -0.4058655854932951 2212 20 -0.6372003180382961 2212 21 -0.161914623364666 2212 22 -0.49741341487528956 2212 23 -0.34774274983511333 2212 24 -0.5204424902240561 2212 25 -0.28638000952140885 2213 1 -0.12273175220301624 2213 2 -0.18334531436868318 2213 3 -0.1468681273338568 2213 4 -0.017728924345270514 2213 5 -0.05548630045922209 2213 6 -0.315366735126851 2213 7 -0.3913926342083869 2213 8 -0.267319601883095 2213 9 -0.31098984175158506 2213 10 -0.23582738886046428 2213 11 0.04957000044579729 2213 12 0.09625225862365028 2213 13 0.08138005632541478 2213 14 -0.6354934289466598 2213 15 -0.20058163561457576 2213 16 -0.3468401110282784 2213 17 -0.1932384372399767 2213 18 -0.19333942675174853 2213 19 -0.26164883854875143 2213 20 -0.3664858697710329 2213 21 -0.0672462172269519 2213 22 -0.35346639625281995 2213 23 -0.5020058019401432 2213 24 -0.7168971189467115 2213 25 -0.4230308487692582 2214 1 -0.22075133676662873 2214 2 -0.11911637648762152 2214 3 -0.15080060684188723 2214 4 -0.08903409957543573 2214 5 -0.09858213920089298 2214 6 -0.030638009779238473 2214 7 -0.1106483681328001 2214 8 -0.16446942792052965 2214 9 -0.10023890652298206 2214 10 -0.06015627989187777 2214 11 0.10448071739738651 2214 12 -0.0969928285422057 2214 13 -0.23464829069637716 2214 14 -0.07144798098966731 2214 15 0.03810401194471069 2214 16 -0.25532641144253887 2214 17 -0.3252050149303187 2214 18 -0.1931981449389113 2214 19 -0.35933890126647583 2214 20 -0.47205086673367447 2214 21 -0.4198136239877428 2214 22 -0.6977732277781215 2214 23 -0.9671212051592519 2214 24 -0.42024887727419724 2214 25 -0.8415385219595848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 14.0 9 29.0 10 126.0 11 299.0 12 830.0 13 2013.0 14 4272.0 15 7228.0 16 10913.0 17 14121.0 18 16490.0 19 18056.0 20 19582.0 21 20675.0 22 22271.0 23 24520.0 24 27459.0 25 30715.0 26 34974.0 27 39132.0 28 46478.0 29 57134.0 30 72574.0 31 93468.0 32 124201.0 33 167346.0 34 226079.0 35 312833.0 36 441616.0 37 687635.0 38 1468735.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57443841602262 20.405540575747857 11.429700541203887 25.590320467025627 2 15.225919618579805 21.46748674413513 38.63625546054455 24.670338176740522 3 18.240005297864396 26.940819443162344 29.73110328026104 25.088071978712218 4 12.035902414581441 17.87885127305753 38.149484826657485 31.93576148570355 5 12.482269112587407 38.82739230106765 35.16011483075193 13.530223755593013 6 30.08047851167116 38.76908614973883 17.61580467487904 13.534630663710962 7 26.02727300972275 33.94708963952361 22.425591047172524 17.60004630358111 8 25.09464757028224 36.41053818951162 20.7882896087867 17.706524631419434 9 25.6669087454748 15.764440869349341 21.344511879689755 37.2241385054861 10 15.040771653590834 29.833447845379563 33.39996157223866 21.725818928790943 11 32.64546080081618 24.884159020180206 23.36404461428745 19.106335564716158 12 21.55605007414021 27.14040244393389 31.364127261126107 19.939420220799793 13 27.985769892314732 22.683198482495946 26.671381310470565 22.659650314718757 14 20.9707065005951 23.09657888862919 28.42609036135071 27.506624249425006 15 23.590760170571293 29.518343027465065 24.84461748972008 22.046279312243566 16 21.130223284507565 29.73383272212401 26.933050220538888 22.202893772829537 17 20.497908596511312 29.15719833850099 28.129846633750994 22.2150464312367 18 20.948126763611878 27.753589350990833 29.883526462194578 21.41475742320272 19 21.18283157898429 28.421957646110997 30.219445191811 20.175765583093717 20 22.219332974599006 27.027074421340487 29.935853692508562 20.81773891155195 21 22.10972254719283 27.3822878520893 29.684875096885776 20.823114503832095 22 21.701272588712694 27.45199671518894 29.171737597909264 21.674993098189095 23 21.586997393416276 27.38506168246775 29.658087862288205 21.369853061827765 24 21.62779506174757 27.64916327577141 29.17759005485472 21.5454516076263 25 21.584383980247246 27.3377796739122 29.443098472924262 21.634737872916297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2521.0 1 2521.0 2 2869.5 3 3218.0 4 3218.0 5 3218.0 6 7406.0 7 11594.0 8 11594.0 9 11594.0 10 16141.5 11 20689.0 12 20689.0 13 20689.0 14 29623.0 15 38557.0 16 38557.0 17 38557.0 18 60015.0 19 81473.0 20 81473.0 21 81473.0 22 115601.0 23 149729.0 24 149729.0 25 149729.0 26 194807.5 27 239886.0 28 239886.0 29 239886.0 30 281415.0 31 322944.0 32 322944.0 33 322944.0 34 353895.5 35 384847.0 36 384847.0 37 384847.0 38 388428.5 39 392010.0 40 392010.0 41 392010.0 42 407427.0 43 422844.0 44 422844.0 45 422844.0 46 460557.5 47 498271.0 48 498271.0 49 498271.0 50 472107.0 51 445943.0 52 445943.0 53 445943.0 54 399613.0 55 353283.0 56 353283.0 57 353283.0 58 305333.5 59 257384.0 60 257384.0 61 257384.0 62 222412.5 63 187441.0 64 187441.0 65 187441.0 66 143771.0 67 100101.0 68 100101.0 69 100101.0 70 74700.0 71 49299.0 72 49299.0 73 49299.0 74 34726.5 75 20154.0 76 20154.0 77 20154.0 78 13452.0 79 6750.0 80 6750.0 81 6750.0 82 4436.5 83 2123.0 84 2123.0 85 2123.0 86 1349.5 87 576.0 88 576.0 89 576.0 90 362.5 91 149.0 92 149.0 93 149.0 94 85.5 95 22.0 96 22.0 97 22.0 98 16.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09662264161342025 2 0.0 3 0.13292189172953275 4 0.011097700346057861 5 0.022571169326858088 6 0.011072649103741703 7 0.019189251614176797 8 0.01743566465204576 9 1.252562115807885E-4 10 0.3889455882006645 11 0.0629537719405043 12 0.001427920812020989 13 0.0 14 0.0011273059042270965 15 0.0014028695697048312 16 1.252562115807885E-4 17 2.5051242316157704E-5 18 2.50512423161577E-4 19 6.012298155877848E-4 20 1.503074538969462E-4 21 1.0020496926463081E-4 22 0.0028057391394096624 23 7.51537269484731E-5 24 2.5051242316157704E-5 25 0.0017786382044471967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3991818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.67463252656542 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66608714336718 41.57006963903478 2 14.90682368269603 16.598638613448077 3 5.088014992889601 8.498200950563515 4 2.1659578102688632 4.823556206190531 5 1.0771129945306976 2.9983935080042525 6 0.5973820906144618 1.9955417023746937 7 0.35668420171942405 1.3900783301132185 8 0.22509277308306466 1.002556594062816 9 0.15610243199462934 0.7821850984023739 >10 0.6895874172655737 6.585786090915774 >50 0.03748561250732704 1.4286377864619086 >100 0.025635476388221463 2.9582064577999643 >500 0.003676289262695096 1.4116835800934848 >1k 0.0037670617000863313 4.160381768166913 >5k 3.177039988024609E-4 1.0277264389956904 >10k+ 2.723177132592522E-4 2.768357235372022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 31553 0.790441848801724 No Hit GGTATCAACGCAGAGTACTTTTTTT 21335 0.5344682548152245 No Hit TATCAACGCAGAGTACTTTTTTTTT 18829 0.4716898415709333 No Hit CCCATGTACTCTGCGTTGATACCAC 13248 0.3318788582044572 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12321 0.30865635657737905 No Hit GTACATGGGAAGCAGTGGTATCAAC 12271 0.3074037944615712 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6350 0.1590753887076014 No Hit GTACTGGTTCACTATCGGTCAGTCA 6335 0.15869962007285904 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6264 0.15692098186841183 No Hit GAGTACATGGGAAGCAGTGGTATCA 5822 0.14584833276467013 No Hit GGATACCACGTGTCCCGCCCTACTC 5528 0.13848326752371976 No Hit CATGTACTCTGCGTTGATACCACTG 5210 0.1305169724671816 No Hit GTTAATGATAGTGTGTCGAAACACA 5163 0.1293395640783222 No Hit AGCGTACACGGTGGATGCCCTGGCA 4459 0.11170348948774719 No Hit GCGTTGATACCACTGCTTCCCATGT 3995 0.10007971305305001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.50512423161577E-5 0.0 2.50512423161577E-5 0.0 5 0.0 5.01024846323154E-5 0.0 5.01024846323154E-5 0.0 6 0.0 5.01024846323154E-5 0.0 5.01024846323154E-5 0.0 7 0.0 5.01024846323154E-5 0.0 5.01024846323154E-5 0.0 8 0.0 5.01024846323154E-5 0.0 5.01024846323154E-5 0.0 9 0.0 5.01024846323154E-5 0.0 1.753586962131039E-4 0.0 10 0.0 5.01024846323154E-5 0.0 2.254611808454193E-4 0.0 11 0.0 5.01024846323154E-5 0.0 2.50512423161577E-4 0.0 12 0.0 5.01024846323154E-5 0.0 2.50512423161577E-4 0.0 13 0.0 5.01024846323154E-5 0.0 2.50512423161577E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATC 90 5.536822E-7 12.642882 17 GTACGAC 55 0.003071884 12.089864 3 GTGCGAC 55 0.0031011621 12.074558 11 ATCGCCG 255 0.0 10.78377 19 AAACCGT 90 9.305547E-5 10.579601 6 TGTCCCG 1175 0.0 10.415683 11 AATCGCC 265 0.0 10.376705 18 CCGCACA 75 0.0026009416 10.15578 9 TACGACA 85 6.461167E-4 10.081739 4 GGAGCGT 95 1.6079385E-4 10.02278 8 TAGACAG 505 0.0 9.804463 5 GTACTGG 1900 0.0 9.709274 1 GAAATCG 305 0.0 9.637607 16 CGCCCTA 1290 0.0 9.555667 16 TAGCGCT 80 0.004434043 9.521643 4 TGGACCG 130 4.121748E-6 9.521642 5 TACTGGT 1780 0.0 9.499059 2 AAATCGC 300 0.0 9.4821615 17 CCCGCCC 1305 0.0 9.445477 14 GTCTTAG 345 0.0 9.37127 1 >>END_MODULE