FastQCFastQC Report
Wed 25 May 2016
SRR1294672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492919
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT147420.4220538752831085No Hit
GGTATCAACGCAGAGTACTTTTTTT100410.2874673017038185No Hit
TATCAACGCAGAGTACTTTTTTTTT90170.25815084747169914No Hit
ATTCCATTCCATTCCATTCCATTCC80480.2304090074805628No Hit
GTACATGGGAAGCAGTGGTATCAAC72100.20641761231794953No Hit
CCCATGTACTCTGCGTTGATACCAC64530.18474519449205665No Hit
GAATGGAATGGAATGGAATGGAATG61650.17649994173927308No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43070.1233066097438847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC350.002175602616.2804728
AACCGTC655.4727916E-513.1496127
CCCGTAC3500.011.67670316
CCCCGTA3550.011.51207815
CCGTACA3800.010.50489617
GGAACCG1002.408889E-510.4467855
GCCCCGT3300.010.36817214
AGCGTAC3550.010.1687991
CTCCGTC750.002655754610.1302169
GCGACTA3800.010.00451916
TAGAACG951.6500324E-49.9970674
ACAGCGT951.6505094E-49.996788
CTAGGAC2002.5465852E-119.9722173
CGACTAA4050.09.85644717
GGTATCA56250.09.6433521
CCGGGGG2002.6375346E-109.50429316
AAGCGAC4100.09.50415814
GAACCGG1901.0459189E-99.50415713
AATACCG800.00452931049.4970775
TACCGTT800.00452984549.4969427
Produced by FastQC (version 0.11.5)