##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294672_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3492919 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.933448213371108 31.0 30.0 33.0 25.0 34.0 2 30.0751597732441 31.0 30.0 34.0 25.0 34.0 3 29.984050875499833 31.0 30.0 34.0 25.0 34.0 4 33.56842572072241 35.0 35.0 37.0 27.0 37.0 5 33.979909640046046 35.0 35.0 37.0 28.0 37.0 6 34.06833682659117 37.0 35.0 37.0 29.0 37.0 7 33.98810879954559 37.0 35.0 37.0 28.0 37.0 8 33.87193691007435 37.0 35.0 37.0 28.0 37.0 9 34.891760444487836 39.0 35.0 39.0 27.0 39.0 10 35.08195151390571 39.0 35.0 39.0 27.0 39.0 11 35.27192557285182 39.0 35.0 39.0 27.0 39.0 12 35.084926675940665 39.0 35.0 39.0 27.0 39.0 13 34.91485488212008 39.0 35.0 39.0 27.0 39.0 14 35.801793571508526 39.0 35.0 41.0 26.0 41.0 15 35.81101708914521 39.0 35.0 41.0 27.0 41.0 16 35.940058443954754 39.0 35.0 41.0 27.0 41.0 17 35.9590331181456 39.0 35.0 41.0 27.0 41.0 18 35.8733082559315 39.0 35.0 41.0 27.0 41.0 19 36.194735692410845 39.0 35.0 41.0 27.0 41.0 20 36.300559217090345 39.0 36.0 41.0 27.0 41.0 21 36.36747860457113 40.0 36.0 41.0 27.0 41.0 22 36.38633389437316 39.0 36.0 41.0 27.0 41.0 23 36.38294103012409 39.0 36.0 41.0 27.0 41.0 24 36.41960520699163 39.0 36.0 41.0 27.0 41.0 25 35.33668458959398 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0385566396001629 1101 2 1.0428429858604957 1101 3 1.3240461718790257 1101 4 1.12299541484024 1101 5 0.814399433479231 1101 6 0.8327845948638455 1101 7 0.8361216557333435 1101 8 0.9741047206890912 1101 9 1.2996982728149433 1101 10 1.1701242502353608 1101 11 0.9160136204550327 1101 12 1.1490900272370865 1101 13 1.1884523848337238 1101 14 1.2926031124668498 1101 15 1.390480828416841 1101 16 1.3447834619704153 1101 17 1.2920154138479987 1101 18 1.440712637355638 1101 19 1.291771759065547 1101 20 1.2213691073478614 1101 21 1.086108691805876 1101 22 1.002864386168433 1101 23 1.0799012788353508 1101 24 1.0446791921332164 1101 25 1.1161329314495774 1102 1 0.921719483451497 1102 2 0.9036553997591632 1102 3 1.1867300967999697 1102 4 1.0698838584261097 1102 5 0.8380278129623235 1102 6 0.8475647182724941 1102 7 0.674067776550622 1102 8 0.8151351732334078 1102 9 1.1080300279730153 1102 10 1.0213779731461514 1102 11 0.8117044757533947 1102 12 1.066884740061326 1102 13 1.0878823749307927 1102 14 1.2234921176782692 1102 15 1.3275412204821322 1102 16 1.2749455289312266 1102 17 1.2898729594623788 1102 18 1.3641094999246022 1102 19 1.0599525475610108 1102 20 0.3528395793628576 1102 21 0.6570452489741783 1102 22 0.6350215506371981 1102 23 0.7930024472250636 1102 24 0.9213026410057381 1102 25 0.9087217599053687 1103 1 0.7410440718513058 1103 2 0.7301286682889341 1103 3 1.0243078492515352 1103 4 0.869507956364636 1103 5 0.6337338007880007 1103 6 0.5603867405457592 1103 7 0.525862882580725 1103 8 0.6398710218437671 1103 9 0.736451468433259 1103 10 0.6636147012756837 1103 11 0.5997861779112483 1103 12 0.889702645593573 1103 13 0.8178225011085303 1103 14 0.72082880057539 1103 15 0.9641462542881882 1103 16 0.9008320694792786 1103 17 0.9207505865639902 1103 18 0.9739862046397079 1103 19 0.8108998784050812 1103 20 -0.0642103063735533 1103 21 0.42074098855372455 1103 22 -0.22036055240401708 1103 23 0.3213782118154782 1103 24 0.49550948469966016 1103 25 0.6735034100311665 1104 1 0.3114297546197484 1104 2 0.34198621546913444 1104 3 0.6253804852857918 1104 4 0.45545603887170927 1104 5 0.28524914697192116 1104 6 0.13528542379508934 1104 7 0.27372100609171923 1104 8 0.3175270991969015 1104 9 0.6196459195057145 1104 10 0.6306191210353873 1104 11 0.3255284332368049 1104 12 0.4062928964259527 1104 13 0.3345729865846323 1104 14 0.6241803293203461 1104 15 0.6356592694526597 1104 16 0.5226197594940771 1104 17 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0.20279331567762426 2207 8 0.3313568131776776 2207 9 -1.3576059547250097 2207 10 -0.20618508963589477 2207 11 0.12823025195070414 2207 12 0.4376164346981284 2207 13 0.5268255924324023 2207 14 0.723462483889513 2207 15 0.7443672337497489 2207 16 0.5506510210600766 2207 17 0.6416899786151689 2207 18 0.6694893323117341 2207 19 -0.48788704526033655 2207 20 0.09236481487349835 2207 21 -0.29728700298581145 2207 22 0.2237843659511114 2207 23 -0.49305953418362947 2207 24 -1.6719208393527794 2207 25 -0.1761938617803196 2208 1 0.49427753208857084 2208 2 0.124399329742225 2208 3 0.6824044412326415 2208 4 0.5352341354631562 2208 5 0.37394568621358104 2208 6 0.29257916774981396 2208 7 0.2960899546264386 2208 8 0.46143140846966446 2208 9 -0.17098613332258594 2208 10 0.0537608420491722 2208 11 0.13170029237726766 2208 12 0.33128355662612563 2208 13 0.4846621198341481 2208 14 0.7664776812525389 2208 15 0.6682214793431243 2208 16 0.570703351981976 2208 17 0.4872673481055827 2208 18 0.706033866682354 2208 19 -1.262737476632502 2208 20 -0.07352750536664843 2208 21 -0.20447796017676723 2208 22 0.24857728639620547 2208 23 0.3143820580405716 2208 24 0.5262142783475596 2208 25 0.5635031561775676 2209 1 0.22744337408811788 2209 2 -1.3177847639439122 2209 3 -0.11396704735804164 2209 4 0.16295083947849776 2209 5 0.1285900864696572 2209 6 0.1501031615069195 2209 7 0.2416954725730065 2209 8 0.3148709532540508 2209 9 -1.9318258562522814 2209 10 -0.24968159532545542 2209 11 0.06837718909171997 2209 12 0.26937690617071297 2209 13 0.5003886070199712 2209 14 0.6781387324692361 2209 15 0.760540209956595 2209 16 0.5893482475642458 2209 17 0.5213175577347045 2209 18 0.6937452986930168 2209 19 0.4750842403612481 2209 20 0.3980557040952917 2209 21 0.4854410680149144 2209 22 0.43850304802796103 2209 23 0.42666878529066565 2209 24 0.5093055345492914 2209 25 0.5374335870715115 2210 1 0.07234819037771345 2210 2 0.190582453979399 2210 3 0.12746016321013443 2210 4 0.1879759363992335 2210 5 0.15256054475528913 2210 6 0.05794259864171636 2210 7 0.05226452227571343 2210 8 0.11459157005692333 2210 9 0.2078330449230421 2210 10 -1.3658802988839227 2210 11 -0.5445811994800849 2210 12 -2.3481657033757912 2210 13 -0.797651466644993 2210 14 -0.4795972878399084 2210 15 -0.7424634138131481 2210 16 -0.22593746922321145 2210 17 0.08803205961348226 2210 18 0.23569225966614482 2210 19 0.14606195297038482 2210 20 0.2923179950390846 2210 21 0.14384294753486415 2210 22 0.16994426364817627 2210 23 0.07959935465748202 2210 24 0.1130850290516392 2210 25 0.047678281851460724 2211 1 -0.40725711250582464 2211 2 -0.2363003815593565 2211 3 -1.6267262807874339 2211 4 -0.6898891806174987 2211 5 -0.5983305535276884 2211 6 -0.5388672437668944 2211 7 -0.4825425655579778 2211 8 -0.1748677645422987 2211 9 -0.03311175580456194 2211 10 4.737517474069364E-4 2211 11 -0.40448411771001247 2211 12 -0.3857945309434996 2211 13 -0.43285043917024524 2211 14 -0.8565555571576198 2211 15 -0.5455219504092739 2211 16 -0.2560477380140611 2211 17 -0.3129802662758081 2211 18 -0.17178658034994498 2211 19 -0.23085170321549242 2211 20 -0.2036389807429302 2211 21 -0.26508275517711866 2211 22 -0.2441715151095778 2211 23 -0.5776871627229667 2211 24 -0.4111390231623986 2211 25 -0.16810487593954804 2212 1 -0.32202536563352524 2212 2 -0.3698733513639283 2212 3 -2.2630973953684546 2212 4 -1.354270899653038 2212 5 -0.5918037452864624 2212 6 -0.34896755460084705 2212 7 -0.2552460942485766 2212 8 -0.3465860685387909 2212 9 -0.8476878155615921 2212 10 -0.23761358427193358 2212 11 -0.33690939526441355 2212 12 -0.020151513635852325 2212 13 -0.39507602097864236 2212 14 -0.08110129614596673 2212 15 -0.0067735192777931275 2212 16 -1.0692935347335322 2212 17 -1.2272873752678493 2212 18 -0.2130154096269692 2212 19 0.010764861882137211 2212 20 -0.0833568429236351 2212 21 0.0161651283908526 2212 22 -0.2631374822914907 2212 23 0.0345466122904412 2212 24 -0.10982678386249489 2212 25 0.02232020277147484 2213 1 -2.9358523748156067 2213 2 -1.2631222793408945 2213 3 -2.372288812587957 2213 4 -1.104359204242165 2213 5 -0.8945972556439017 2213 6 -0.6708700982517755 2213 7 -0.46663278483195825 2213 8 -0.17348699770521137 2213 9 -0.3751179605141033 2213 10 -0.24957393551141394 2213 11 -0.6206632925023428 2213 12 -0.3858617777676514 2213 13 -0.7550971555649468 2213 14 -0.34482401548046937 2213 15 -0.36670541835309933 2213 16 -1.6965121210408967 2213 17 -1.129056632184728 2213 18 -0.47653066637256103 2213 19 -0.38508333295396113 2213 20 -0.318455576497513 2213 21 -0.15795077510606603 2213 22 -0.18325919899653798 2213 23 -0.05800617230966765 2213 24 -0.12554433578267066 2213 25 -0.1886356039733954 2214 1 -1.0348908698419805 2214 2 -0.9300738611759805 2214 3 -6.543529258486224 2214 4 -2.359506162601953 2214 5 -1.4247149455672528 2214 6 -0.8895037110082598 2214 7 -0.7003322080361372 2214 8 -0.6336339694601634 2214 9 -0.38841708259496244 2214 10 -0.47778723476744744 2214 11 -1.0280878308379613 2214 12 -0.773511435253269 2214 13 -5.050390764195718 2214 14 -3.86864410233472 2214 15 -2.439728626816695 2214 16 -2.972694043461459 2214 17 -3.769672515100268 2214 18 -1.4890744350755654 2214 19 -1.0234409249318617 2214 20 -0.7254079352521927 2214 21 -0.6733972761549083 2214 22 -0.7317795690245887 2214 23 -0.6680721033138468 2214 24 -0.6113292522906022 2214 25 -0.27986404780345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1350.0 3 161.0 4 159.0 5 390.0 6 40.0 7 53.0 8 97.0 9 272.0 10 742.0 11 1902.0 12 4129.0 13 8072.0 14 13059.0 15 19073.0 16 24460.0 17 28439.0 18 30314.0 19 30622.0 20 29905.0 21 29245.0 22 28285.0 23 28012.0 24 28834.0 25 30282.0 26 32949.0 27 36979.0 28 42611.0 29 51894.0 30 64924.0 31 80565.0 32 106242.0 33 143532.0 34 195569.0 35 268737.0 36 384152.0 37 588653.0 38 1158215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.208526155182945 23.508656492627395 13.239424268442404 21.043393083747254 2 13.451370679585064 23.84351147880776 42.72085526327097 19.98426257833621 3 16.454990579920004 31.962931548373554 31.314969834832784 20.267108036873662 4 10.494977355203492 18.867952091739856 41.31346980841795 29.323600744638707 5 11.56814702957365 40.591730789501426 35.20552724322987 12.634594937695063 6 26.53302633414724 42.302344922581256 19.088869044907657 12.075759698363846 7 21.950200776521136 36.52600039476357 26.585063097713725 14.938735731001573 8 21.782306135684966 40.11095476327435 23.03597579002129 15.070763311019391 9 23.68965214954974 17.993616066747684 23.98799302591675 34.32873875778583 10 14.190323699776341 32.25082943931593 33.791591214260194 19.767255646647534 11 29.5885570104124 29.722794196003477 23.9637439283885 16.724904865195615 12 18.04479594546476 31.13472042424574 34.0575975698456 16.7628860604439 13 23.883933303273245 27.831898030987862 28.907271886618087 19.376896779120806 14 18.34337376146647 25.220864113746128 30.651375460828866 25.784386663958536 15 21.398932796427452 32.60987022928318 25.666515213065423 20.324681761223946 16 19.545571345946104 34.225984236855666 27.512596679100298 18.71584773809793 17 17.41517669397376 33.026257438404336 31.083735262133207 18.474830605488695 18 17.876016432061498 32.26562394730135 31.81287931196118 18.04548030867597 19 18.79741769282646 30.60625316213449 32.075001962457186 18.52132718258186 20 20.869604576486235 29.682818381230426 30.577936370019536 18.869640672263806 21 21.230685229433934 31.15982155313401 29.721034253880283 17.888458963551773 22 19.217746369041258 30.543197787662717 31.927140465827293 18.311915377468733 23 18.93506177260519 31.09506177260519 31.61637242614145 18.353504028648164 24 18.9020683095101 29.385237329849385 31.575628386179943 20.137065974460576 25 19.865280894949237 29.55122471653237 30.587258868444316 19.996235520074073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4142.0 1 4142.0 2 4689.5 3 5237.0 4 5237.0 5 5237.0 6 8947.0 7 12657.0 8 12657.0 9 12657.0 10 20177.5 11 27698.0 12 27698.0 13 27698.0 14 43937.0 15 60176.0 16 60176.0 17 60176.0 18 87988.5 19 115801.0 20 115801.0 21 115801.0 22 155789.0 23 195777.0 24 195777.0 25 195777.0 26 246946.5 27 298116.0 28 298116.0 29 298116.0 30 345003.5 31 391891.0 32 391891.0 33 391891.0 34 425063.0 35 458235.0 36 458235.0 37 458235.0 38 470205.0 39 482175.0 40 482175.0 41 482175.0 42 450216.0 43 418257.0 44 418257.0 45 418257.0 46 393464.5 47 368672.0 48 368672.0 49 368672.0 50 317498.0 51 266324.0 52 266324.0 53 266324.0 54 216693.5 55 167063.0 56 167063.0 57 167063.0 58 134506.5 59 101950.0 60 101950.0 61 101950.0 62 82558.5 63 63167.0 64 63167.0 65 63167.0 66 47259.0 67 31351.0 68 31351.0 69 31351.0 70 23098.5 71 14846.0 72 14846.0 73 14846.0 74 10546.5 75 6247.0 76 6247.0 77 6247.0 78 4203.5 79 2160.0 80 2160.0 81 2160.0 82 1422.5 83 685.0 84 685.0 85 685.0 86 437.5 87 190.0 88 190.0 89 190.0 90 123.0 91 56.0 92 56.0 93 56.0 94 39.0 95 22.0 96 22.0 97 22.0 98 23.0 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1006321646737299 2 0.053737289642273416 3 0.05645707787669854 4 0.052391710199978875 5 0.048755782770800005 6 0.06745074821374329 7 0.06361441533571205 8 0.06089462710128692 9 0.06530354697603924 10 0.06607653942161269 11 0.059634935708500536 12 0.060465186853746106 13 0.06106640320030324 14 0.06292731093964675 15 0.06587613397276032 16 0.06063696295276243 17 0.06444466648095762 18 0.13913864020322259 19 0.0686245515570215 20 0.06315634573833519 21 0.061037773850467186 22 0.06619105682095691 23 0.06567572852390795 24 0.07526656071898603 25 0.0686245515570215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3492919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.07557558825688 #Duplication Level Percentage of deduplicated Percentage of total 1 83.30483772387205 57.543296150627974 2 11.502695680689223 15.891106499203286 3 2.8707969735141052 5.94905860027538 4 0.9812426741243493 2.7111961002759917 5 0.42317452493742674 1.4615511942169959 6 0.2086615725320296 0.8648050935480455 7 0.13561839226115535 0.6557542954055315 8 0.09426454258294327 0.5209102029184446 9 0.06841792375730321 0.4253406717680275 >10 0.35234617418506003 4.530786073866808 >50 0.03126906170992647 1.4935169444011736 >100 0.02198298705541235 3.037296808428742 >500 0.0028485739160549503 1.3008895188994092 >1k 0.0015499593166524038 1.829816241706523 >5k 2.0945396170978425E-4 1.067547134627091 >10k+ 8.37815846839137E-5 0.7171284698306779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14742 0.4220538752831085 No Hit GGTATCAACGCAGAGTACTTTTTTT 10041 0.2874673017038185 No Hit TATCAACGCAGAGTACTTTTTTTTT 9017 0.25815084747169914 No Hit ATTCCATTCCATTCCATTCCATTCC 8048 0.2304090074805628 No Hit GTACATGGGAAGCAGTGGTATCAAC 7210 0.20641761231794953 No Hit CCCATGTACTCTGCGTTGATACCAC 6453 0.18474519449205665 No Hit GAATGGAATGGAATGGAATGGAATG 6165 0.17649994173927308 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4307 0.1233066097438847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.725869967210806E-5 0.0 5 0.0 0.0 0.0 5.725869967210806E-5 0.0 6 0.0 0.0 0.0 5.725869967210806E-5 0.0 7 0.0 0.0 0.0 5.725869967210806E-5 0.0 8 0.0 0.0 0.0 5.725869967210806E-5 0.0 9 0.0 0.0 0.0 1.7177609901632417E-4 0.0 10 0.0 0.0 0.0 1.7177609901632417E-4 0.0 11 0.0 0.0 0.0 1.7177609901632417E-4 0.0 12 0.0 0.0 0.0 2.004054488523782E-4 0.0 13 0.0 0.0 0.0 2.004054488523782E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 35 0.0021756026 16.280472 8 AACCGTC 65 5.4727916E-5 13.149612 7 CCCGTAC 350 0.0 11.676703 16 CCCCGTA 355 0.0 11.512078 15 CCGTACA 380 0.0 10.504896 17 GGAACCG 100 2.408889E-5 10.446785 5 GCCCCGT 330 0.0 10.368172 14 AGCGTAC 355 0.0 10.168799 1 CTCCGTC 75 0.0026557546 10.130216 9 GCGACTA 380 0.0 10.004519 16 TAGAACG 95 1.6500324E-4 9.997067 4 ACAGCGT 95 1.6505094E-4 9.99678 8 CTAGGAC 200 2.5465852E-11 9.972217 3 CGACTAA 405 0.0 9.856447 17 GGTATCA 5625 0.0 9.643352 1 CCGGGGG 200 2.6375346E-10 9.504293 16 AAGCGAC 410 0.0 9.504158 14 GAACCGG 190 1.0459189E-9 9.504157 13 AATACCG 80 0.0045293104 9.497077 5 TACCGTT 80 0.0045298454 9.496942 7 >>END_MODULE