FastQCFastQC Report
Wed 25 May 2016
SRR1294672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492919
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT390271.1173176360516806No Hit
GGTATCAACGCAGAGTACTTTTTTT291990.8359483858629415No Hit
TATCAACGCAGAGTACTTTTTTTTT256250.7336270895488844No Hit
ACGCAGAGTACTTTTTTTTTTTTTT155590.4454440540991646No Hit
GTACTTTTTTTTTTTTTTTTTTTTT86130.24658459013793335No Hit
GAGTACTTTTTTTTTTTTTTTTTTT79640.22800414209433428No Hit
CCCATGTACTCTGCGTTGATACCAC76010.21761168810384668No Hit
ATTCCATTCCATTCCATTCCATTCC75610.21646651411040452No Hit
GTACATGGGAAGCAGTGGTATCAAC72320.20704745801434274No Hit
GAATGGAATGGAATGGAATGGAATG69370.19860179981270681No Hit
GCAGAGTACTTTTTTTTTTTTTTTT49810.14260279153338512No Hit
ATCAACGCAGAGTACTTTTTTTTTT38110.1091064522252019No Hit
GAGTACATGGGAAGCAGTGGTATCA35410.1013765277694673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT500.001515121513.28254111
TCGGAAC751.5101417E-512.64170913
GGTATCA78950.011.8840671
CCCCGTA3300.011.49229915
CCCGTAC3300.011.49229916
ATCGCCG1053.5525954E-610.83575219
CCGTACA3450.010.71781817
AATCGCC1106.1961637E-610.34306918
AAATCGC1201.5646383E-610.27124317
TACCGTA750.002595755310.1581577
CTCGTAG750.002635031210.1397871
TATTCCG2450.09.7176265
TAGACAG5000.09.71373755
AGCGCTC800.0044275519.5232728
CGCAGCG1606.397386E-89.5232728
CCAGGAC2600.09.4974623
GCTCGAA800.004567049.4875311
TCGCCCA1304.3494274E-69.48141812
GCCCCGT3550.09.34774414
GTATTAG5300.09.3266911
Produced by FastQC (version 0.11.5)