##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294672_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3492919 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.239255476579903 31.0 31.0 34.0 28.0 34.0 2 31.419986836224947 31.0 31.0 34.0 28.0 34.0 3 31.483245675035693 31.0 31.0 34.0 28.0 34.0 4 35.02867000351282 37.0 35.0 37.0 32.0 37.0 5 35.19005250336466 37.0 35.0 37.0 32.0 37.0 6 34.71594588938363 37.0 35.0 37.0 32.0 37.0 7 34.56357590886018 37.0 35.0 37.0 31.0 37.0 8 34.60008520094511 37.0 35.0 37.0 31.0 37.0 9 36.03490461702662 39.0 35.0 39.0 30.0 39.0 10 35.82568046954424 39.0 35.0 39.0 30.0 39.0 11 35.96847250108004 39.0 35.0 39.0 30.0 39.0 12 35.93571050459516 39.0 35.0 39.0 30.0 39.0 13 35.77768651377258 39.0 35.0 39.0 30.0 39.0 14 36.81162259989424 40.0 36.0 41.0 30.0 41.0 15 36.86811202893626 40.0 36.0 41.0 30.0 41.0 16 36.91500604508722 40.0 36.0 41.0 30.0 41.0 17 36.994016179590766 40.0 36.0 41.0 30.0 41.0 18 37.023237298087935 40.0 36.0 41.0 30.0 41.0 19 37.07137325543478 40.0 37.0 41.0 30.0 41.0 20 37.13789040055037 40.0 37.0 41.0 30.0 41.0 21 36.987161740653015 40.0 36.0 41.0 30.0 41.0 22 36.96940209606922 40.0 36.0 41.0 30.0 41.0 23 36.984045149629864 40.0 36.0 41.0 30.0 41.0 24 36.91937116205672 40.0 36.0 41.0 30.0 41.0 25 35.86319551068891 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5276344978857708 1101 2 0.5021079308791769 1101 3 0.47556407779241283 1101 4 0.46349247028759066 1101 5 0.43480345700004364 1101 6 0.5142334477332611 1101 7 0.5354478081649603 1101 8 0.5160826194788513 1101 9 0.7295451534035493 1101 10 0.805486511093882 1101 11 0.7398666217195355 1101 12 0.7467165486305731 1101 13 0.7330482151445281 1101 14 1.0268671636972897 1101 15 0.9662670681116978 1101 16 0.988026630204665 1101 17 1.0574810680133595 1101 18 0.941919853371374 1101 19 1.129002379949469 1101 20 0.9878327852387514 1101 21 1.2195797750828277 1101 22 1.1332620101057032 1101 23 1.152966387924394 1101 24 1.2853289575942668 1101 25 1.2017525396891386 1102 1 0.36162291361381804 1102 2 0.3327663735302444 1102 3 0.2960618417182488 1102 4 0.2549656359079151 1102 5 0.36140978802468027 1102 6 0.3332647423434807 1102 7 0.4066048582974915 1102 8 0.3541137708894482 1102 9 0.552912822057877 1102 10 0.668650406130979 1102 11 0.6054283655221937 1102 12 0.6904724074316562 1102 13 0.7141173045848888 1102 14 0.8789215195913584 1102 15 0.778224413246619 1102 16 0.8377397002518094 1102 17 0.7397296781949478 1102 18 0.6880913351950824 1102 19 0.8808298510367436 1102 20 0.7923501047578796 1102 21 1.0852254787404334 1102 22 1.037547170267679 1102 23 0.8990684579689372 1102 24 1.0044909474619175 1102 25 0.978456615161484 1103 1 0.29987198309731156 1103 2 0.3110736612704841 1103 3 0.2820761934017533 1103 4 0.24264737856799456 1103 5 0.26615120938679837 1103 6 0.3137878271350374 1103 7 0.3029326440081874 1103 8 0.36391505129198976 1103 9 0.22477585801025413 1103 10 0.5231899445829669 1103 11 0.5270061120610237 1103 12 0.5232893004082158 1103 13 0.6102806423823353 1103 14 0.7394741237072111 1103 15 0.7335817936689608 1103 16 0.6602283404116918 1103 17 0.6477556232715571 1103 18 0.644281487241031 1103 19 0.7857597407938144 1103 20 0.7278431011245061 1103 21 0.9735598024566983 1103 22 0.8610394970171811 1103 23 0.8794058459595817 1103 24 0.9850084890653932 1103 25 0.8622787837749115 1104 1 0.24030498372503573 1104 2 0.2374436399425761 1104 3 0.1849669328304877 1104 4 0.19989483004791708 1104 5 0.20400742403147376 1104 6 0.13805355600994318 1104 7 0.24230301179533598 1104 8 0.2244950346261163 1104 9 0.13712796675689987 1104 10 0.42070263670852626 1104 11 0.46920077604548993 1104 12 0.5513433029705013 1104 13 0.06817573182373593 1104 14 0.37919809062508847 1104 15 0.5369708573333298 1104 16 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2207 1 0.1947381526597134 2207 2 0.2572282863133246 2207 3 0.25182769319578213 2207 4 0.22081477392858773 2207 5 0.163813244260254 2207 6 0.30746722950217276 2207 7 0.19555483438250576 2207 8 0.2785447501561933 2207 9 0.3269606416348907 2207 10 0.5444341418599734 2207 11 0.4435433966116733 2207 12 0.37740780577216526 2207 13 0.49842465376449496 2207 14 0.4180594362840182 2207 15 0.4083598968492552 2207 16 0.6324290133717838 2207 17 0.624142305308304 2207 18 0.6075864690150325 2207 19 0.6098527678333809 2207 20 0.532288393692518 2207 21 0.895485008343023 2207 22 0.7934557318331699 2207 23 0.622792905324772 2207 24 0.7747011913947901 2207 25 0.7728620677786111 2208 1 0.25079908045977106 2208 2 0.22677033846407113 2208 3 0.23519569395508455 2208 4 0.23907541827618672 2208 5 0.1707565425079025 2208 6 0.3685574275488861 2208 7 0.4122239945299242 2208 8 0.3059430362501345 2208 9 0.4651355102445436 2208 10 0.5574573505353513 2208 11 0.20705323620847338 2208 12 0.05981428817864298 2208 13 0.1324618725843223 2208 14 -0.21046654658978525 2208 15 0.42378271879381657 2208 16 0.4084399589479446 2208 17 0.31456299572899127 2208 18 0.4682019405000659 2208 19 0.6826661058641079 2208 20 0.5118099993010787 2208 21 0.8159379853177384 2208 22 0.798623671783723 2208 23 0.7866851322604731 2208 24 0.8555208548231477 2208 25 0.7479946646503421 2209 1 0.06622098506549534 2209 2 0.04736810697812288 2209 3 0.1150434719915765 2209 4 -0.4783242530681875 2209 5 -0.05202984105785191 2209 6 0.1626401839977305 2209 7 0.16922290745785773 2209 8 0.23809506123063784 2209 9 0.2670071037812676 2209 10 0.25140237825242195 2209 11 0.3392454068352819 2209 12 0.33670928141572176 2209 13 0.44917767344215775 2209 14 0.43713412551112185 2209 15 -0.21769665863151744 2209 16 -0.18958080012829726 2209 17 0.18253661116239073 2209 18 -0.15315301729600606 2209 19 0.3117461123600904 2209 20 0.3123861427149066 2209 21 0.5728864572093357 2209 22 0.6482844418693361 2209 23 0.5800255369011751 2209 24 0.6962955956733623 2209 25 0.7770635629623257 2210 1 -0.17305975446183552 2210 2 -0.21856108176223898 2210 3 -0.18002946171894862 2210 4 -0.1264621112465676 2210 5 -0.08090853604006298 2210 6 -0.48215039259169856 2210 7 -0.23654700990491762 2210 8 -0.3544464321083254 2210 9 -0.15361294911198087 2210 10 0.04286830505098038 2210 11 0.018357590347058306 2210 12 -0.015534343067137968 2210 13 -0.013019690351733004 2210 14 -0.04230821089189618 2210 15 -0.053274752376225365 2210 16 0.11208675082717434 2210 17 -0.9856609558364866 2210 18 -1.5548785952666933 2210 19 -0.5043387380294462 2210 20 -0.24339236510729734 2210 21 -0.4595612620749634 2210 22 -0.4084663497702934 2210 23 -0.2981438417159481 2210 24 0.07900789953045262 2210 25 0.07979450866857007 2211 1 -0.2754651131363026 2211 2 -0.2855565442145256 2211 3 -0.2609400541429352 2211 4 -0.13645014169037495 2211 5 -0.135234665714421 2211 6 -0.05934128343994871 2211 7 -0.23453190174136296 2211 8 -0.14411598362133304 2211 9 -0.1954472384546122 2211 10 -0.14467357593507302 2211 11 -0.3684791580289186 2211 12 -0.22617716518600162 2211 13 -0.19397461809683847 2211 14 -0.27641466011230875 2211 15 -0.2681441172004426 2211 16 -0.3009775960982708 2211 17 -0.4652230346417454 2211 18 -0.4733373725286043 2211 19 -0.33126497332090565 2211 20 -0.3769825889156948 2211 21 -0.037377308732125414 2211 22 -0.19342286299747258 2211 23 -0.6925032956021866 2211 24 -0.26385823598936753 2211 25 -0.8812026650976961 2212 1 -0.1938193764638534 2212 2 -0.2968033214918684 2212 3 -0.15684909472629016 2212 4 -0.13030217341477623 2212 5 -0.07469782538289138 2212 6 -0.1662873876486728 2212 7 -0.2161291489995918 2212 8 -0.3572102355987923 2212 9 -0.1562494396883949 2212 10 -0.527265123235523 2212 11 -0.12805987782178363 2212 12 -0.32994584686451134 2212 13 -0.267115466492605 2212 14 -0.19899119303075707 2212 15 -0.06736099302258225 2212 16 -0.369825231335291 2212 17 -0.6853757447757829 2212 18 -0.35285078764864153 2212 19 -0.497203313751001 2212 20 -0.6824335021614516 2212 21 -0.21544640824876637 2212 22 -0.48818854981110604 2212 23 -0.3691776677778762 2212 24 -0.5498628190182728 2212 25 -0.2505661811213358 2213 1 -0.2452129133126668 2213 2 -0.2353298955876646 2213 3 -0.1457430637888315 2213 4 -0.01536098373917838 2213 5 -0.13120504907065111 2213 6 -0.30887708995049934 2213 7 -0.4125931619537724 2213 8 -0.3692489172644713 2213 9 -0.360232508613322 2213 10 -0.2880566355686369 2213 11 -0.09801636919320345 2213 12 -0.013717272031421146 2213 13 0.01261299157628315 2213 14 -0.6849087388866408 2213 15 -0.3377995157400022 2213 16 -0.2985884298126251 2213 17 -0.3098612774519367 2213 18 -0.3125733888890565 2213 19 -0.3243486764035879 2213 20 -0.40940648468086493 2213 21 -0.11624777239508433 2213 22 -0.4659085306394388 2213 23 -0.5546496212296574 2213 24 -0.8592052317656282 2213 25 -0.5470146609791087 2214 1 -0.36866891957088654 2214 2 -0.32595421745741504 2214 3 -0.23637408324129083 2214 4 -0.23691943086444667 2214 5 -0.2283687567452759 2214 6 -0.1942756898878102 2214 7 -0.208199145842066 2214 8 -0.4002469374590234 2214 9 -0.42824297827461066 2214 10 -0.20375590338889538 2214 11 -0.17620689666802747 2214 12 -0.3359154722569997 2214 13 -0.36849339550649063 2214 14 -0.41498061734426983 2214 15 -0.24006477216364885 2214 16 -0.5382346367036348 2214 17 -0.4535600576881791 2214 18 -0.44158396839070235 2214 19 -0.7307622816051946 2214 20 -0.5253452580587776 2214 21 -0.7094420907017209 2214 22 -0.9335092421906239 2214 23 -1.2385779678108122 2214 24 -0.7819855858802214 2214 25 -0.9548654334783038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 45.0 10 123.0 11 280.0 12 707.0 13 1663.0 14 3546.0 15 6245.0 16 9351.0 17 12516.0 18 15185.0 19 17001.0 20 18100.0 21 19272.0 22 20141.0 23 21533.0 24 23040.0 25 25845.0 26 29420.0 27 32851.0 28 38365.0 29 47292.0 30 60342.0 31 77953.0 32 104441.0 33 141460.0 34 192234.0 35 266865.0 36 381096.0 37 605091.0 38 1320904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48134775307623 23.63617085905217 12.249875346022248 22.63260604184935 2 13.341677834498883 24.023488663779492 41.51802546809702 21.116808033624597 3 16.117325538693354 31.811482626184386 31.764352422690266 20.306839412431994 4 10.362520723037145 18.77144959613342 41.33898737004258 29.52704231078686 5 11.726532488191253 39.864637905681796 35.373749146291 13.035080459835946 6 26.85738173842581 42.28683424624979 18.29415857544947 12.561625439874932 7 21.85080485588077 36.74887133473723 26.182084647460695 15.218239161921298 8 22.56378376103056 39.27305454481937 22.614925882581012 15.548235811569059 9 24.064498600451827 16.392535399536147 24.47885189238896 35.06411410762306 10 14.537114940344804 31.55802580636631 33.77650866981573 20.12835058347315 11 30.14567296935479 28.450848515180798 23.863907544403915 17.5395709710605 12 18.6173295038112 30.519310227939023 34.310697467751396 16.55266280049839 13 24.748297913578874 26.71378866787349 29.21427608255445 19.32363733599319 14 18.862953219312583 24.243423680162437 31.54025353913526 25.353369561389723 15 21.774210633903504 31.36584684325136 26.698161337995792 20.161781184849346 16 19.334665171069005 33.30204144500386 28.359469074281158 19.003824309645974 17 17.181274458411433 32.3113418891191 31.799964442347505 18.707419210121966 18 17.53386294040718 31.088458199824558 33.230200429213056 18.147478430555207 19 18.387997588249068 29.46003576399883 34.097846544617106 18.05412010313499 20 20.39735360984211 28.42267004379444 32.71854491485463 18.46143143150881 21 20.701432731695196 30.032855145340214 31.622304395077112 17.64340772788748 22 18.591135647943098 29.62672630174253 33.76873390080563 18.013404149508748 23 18.43390799250253 30.23652764127113 33.47800731252528 17.851557053701054 24 18.32695089146146 28.49693493917403 33.627347465556 19.54876670380851 25 19.333486217913208 28.5882426032064 32.580620133066844 19.497651045813555 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2700.0 1 2700.0 2 3379.5 3 4059.0 4 4059.0 5 4059.0 6 9791.5 7 15524.0 8 15524.0 9 15524.0 10 21552.5 11 27581.0 12 27581.0 13 27581.0 14 38972.0 15 50363.0 16 50363.0 17 50363.0 18 78512.5 19 106662.0 20 106662.0 21 106662.0 22 149603.5 23 192545.0 24 192545.0 25 192545.0 26 247679.0 27 302813.0 28 302813.0 29 302813.0 30 352787.0 31 402761.0 32 402761.0 33 402761.0 34 433940.0 35 465119.0 36 465119.0 37 465119.0 38 471258.5 39 477398.0 40 477398.0 41 477398.0 42 446546.0 43 415694.0 44 415694.0 45 415694.0 46 393309.5 47 370925.0 48 370925.0 49 370925.0 50 320159.0 51 269393.0 52 269393.0 53 269393.0 54 218860.5 55 168328.0 56 168328.0 57 168328.0 58 135233.5 59 102139.0 60 102139.0 61 102139.0 62 83106.0 63 64073.0 64 64073.0 65 64073.0 66 47654.0 67 31235.0 68 31235.0 69 31235.0 70 23061.0 71 14887.0 72 14887.0 73 14887.0 74 10342.0 75 5797.0 76 5797.0 77 5797.0 78 3936.0 79 2075.0 80 2075.0 81 2075.0 82 1347.0 83 619.0 84 619.0 85 619.0 86 391.5 87 164.0 88 164.0 89 164.0 90 105.5 91 47.0 92 47.0 93 47.0 94 31.5 95 16.0 96 16.0 97 16.0 98 9.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09330305111570007 2 0.0 3 0.13484423772781448 4 0.012281991079667178 5 0.02301799726818744 6 0.012854578076388259 7 0.021214348228516037 8 0.01975425138687728 9 1.7177609901632417E-4 10 0.401097191203117 11 0.06561846982423583 12 0.0015746142409829714 13 0.0 14 0.0013169500924584852 15 0.0015173555413108635 16 2.862934983605403E-5 17 0.0 18 4.2944024754081045E-4 19 6.012163465571347E-4 20 2.862934983605403E-4 21 3.149228481965943E-4 22 0.0034355219803264836 23 2.2903479868843223E-4 24 8.588804950816208E-5 25 0.0025193827855727545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3492919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.5671328618844 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07911314501264 53.81692116764686 2 12.415991627348404 16.28161945284794 3 3.0335235258009705 5.966983201675327 4 1.0188550602401278 2.6721362040707075 5 0.4618843937449315 1.5142217705752425 6 0.23472745021183164 0.9234243548622312 7 0.15406068707245446 0.7070922276650973 8 0.09509739171041248 0.49882106536762233 9 0.07523313584819133 0.4439538912397103 >10 0.3684503150837473 4.511848821433159 >50 0.033561874350644394 1.5338988262708653 >100 0.02440301653083891 3.245636166157074 >500 0.0029447522625840263 1.3275187308487273 >1k 0.0017141095208274499 2.0800079609561535 >5k 2.637091570503769E-4 1.322965522341643 >10k+ 1.7580610470025127E-4 3.15295063604163 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 39027 1.1173176360516806 No Hit GGTATCAACGCAGAGTACTTTTTTT 29199 0.8359483858629415 No Hit TATCAACGCAGAGTACTTTTTTTTT 25625 0.7336270895488844 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 15559 0.4454440540991646 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 8613 0.24658459013793335 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7964 0.22800414209433428 No Hit CCCATGTACTCTGCGTTGATACCAC 7601 0.21761168810384668 No Hit ATTCCATTCCATTCCATTCCATTCC 7561 0.21646651411040452 No Hit GTACATGGGAAGCAGTGGTATCAAC 7232 0.20704745801434274 No Hit GAATGGAATGGAATGGAATGGAATG 6937 0.19860179981270681 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4981 0.14260279153338512 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3811 0.1091064522252019 No Hit GAGTACATGGGAAGCAGTGGTATCA 3541 0.1013765277694673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.862934983605403E-5 0.0 8 0.0 0.0 0.0 2.862934983605403E-5 0.0 9 0.0 0.0 0.0 1.4314674918027015E-4 0.0 10 0.0 0.0 0.0 1.4314674918027015E-4 0.0 11 0.0 0.0 0.0 2.004054488523782E-4 0.0 12 0.0 0.0 0.0 2.004054488523782E-4 0.0 13 0.0 0.0 0.0 2.004054488523782E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATT 50 0.0015151215 13.282541 11 TCGGAAC 75 1.5101417E-5 12.641709 13 GGTATCA 7895 0.0 11.884067 1 CCCCGTA 330 0.0 11.492299 15 CCCGTAC 330 0.0 11.492299 16 ATCGCCG 105 3.5525954E-6 10.835752 19 CCGTACA 345 0.0 10.717818 17 AATCGCC 110 6.1961637E-6 10.343069 18 AAATCGC 120 1.5646383E-6 10.271243 17 TACCGTA 75 0.0025957553 10.158157 7 CTCGTAG 75 0.0026350312 10.139787 1 TATTCCG 245 0.0 9.717626 5 TAGACAG 500 0.0 9.7137375 5 AGCGCTC 80 0.004427551 9.523272 8 CGCAGCG 160 6.397386E-8 9.523272 8 CCAGGAC 260 0.0 9.497462 3 GCTCGAA 80 0.00456704 9.48753 11 TCGCCCA 130 4.3494274E-6 9.481418 12 GCCCCGT 355 0.0 9.347744 14 GTATTAG 530 0.0 9.326691 1 >>END_MODULE