FastQCFastQC Report
Wed 25 May 2016
SRR1294671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573212
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC178840.500502069286681No Hit
CCCATGTACTCTGCGTTGATACCAC162190.4539053378305009No Hit
GTATCAACGCAGAGTACTTTTTTTT144260.4037263951872993No Hit
GGTATCAACGCAGAGTACTTTTTTT85610.23958835915697138No Hit
TATCAACGCAGAGTACTTTTTTTTT80190.22441993366192658No Hit
GAGTACATGGGAAGCAGTGGTATCA77050.21563232184376413No Hit
CATGTACTCTGCGTTGATACCACTG59840.1674683729932621No Hit
GTATCAACGCAGAGTACATGGGAAG54060.15129245060186747No Hit
GCGTTGATACCACTGCTTCCCATGT52120.14586316177153777No Hit
ACGCAGAGTACATGGGAAGCAGTGG43680.12224295675711376No Hit
GGTATCAACGCAGAGTACATGGGAA43070.12053580923829876No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38500.10774619585963553No Hit
ATTCCATTCCATTCCATTCCATTCC37410.1046957191456874No Hit
TATCAACGCAGAGTACATGGGAAGC36970.10346433405014872No Hit
GTGGTATCAACGCAGAGTACATGGG36030.1008336477096797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTG550.003076113312.0875855
AGGACCG750.002655058210.13054855
CGCCCTA1901.0459189E-99.50417516
TCACGGT1002.7596598E-49.4991181
GTCTTAG3400.09.4991181
TAGGACC2303.6379788E-129.4973894
GTCTAGG3450.09.3614491
GTAGGAC1856.7411747E-99.2404433
GTACTGG6450.09.1309351
CACGGTA950.00182970168.9972732
CCGAGAC950.00182970168.9972733
TGCACTG6050.08.9479525
TTAGACA4800.08.9038014
TGTCCCG2054.1691237E-98.80221111
ATTAGAC3150.08.7433833
ACTGTCC3600.08.7056968
CCGTGTG1201.7706845E-48.7056959
GTATTAG5250.08.6849071
GTACAAG4400.08.6355621
TATTCCG1107.167626E-48.6339895
Produced by FastQC (version 0.11.5)