##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294671_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3573212 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.099402722256613 31.0 30.0 33.0 26.0 34.0 2 30.2549543659878 31.0 30.0 34.0 26.0 34.0 3 30.21757035406799 31.0 30.0 34.0 25.0 34.0 4 33.816954605548176 37.0 35.0 37.0 28.0 37.0 5 34.209719434503185 37.0 35.0 37.0 30.0 37.0 6 34.26755759244064 37.0 35.0 37.0 30.0 37.0 7 34.20334645691327 37.0 35.0 37.0 30.0 37.0 8 34.00849207939523 37.0 35.0 37.0 28.0 37.0 9 35.13193647620125 39.0 35.0 39.0 27.0 39.0 10 35.3044300198253 39.0 35.0 39.0 28.0 39.0 11 35.496137648703744 39.0 35.0 39.0 28.0 39.0 12 35.305856747374634 39.0 35.0 39.0 27.0 39.0 13 35.141372244356056 39.0 35.0 39.0 27.0 39.0 14 36.08324275189941 39.0 36.0 41.0 27.0 41.0 15 36.08490456205789 39.0 36.0 41.0 27.0 41.0 16 36.21269798713315 39.0 36.0 41.0 27.0 41.0 17 36.213967713082795 39.0 36.0 41.0 27.0 41.0 18 36.126766058101225 39.0 36.0 41.0 27.0 41.0 19 36.41763209123892 40.0 36.0 41.0 27.0 41.0 20 36.535301012086606 40.0 36.0 41.0 27.0 41.0 21 36.598937034802304 40.0 36.0 41.0 27.0 41.0 22 36.61090329932845 40.0 36.0 41.0 28.0 41.0 23 36.61660545190154 39.0 36.0 41.0 28.0 41.0 24 36.640337880875805 40.0 36.0 41.0 28.0 41.0 25 35.556960796056885 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8981649947806432 1101 2 0.9355394686819238 1101 3 1.229320360857205 1101 4 0.9527385748005912 1101 5 0.7660333995459112 1101 6 0.714873263173267 1101 7 0.6371557403301367 1101 8 0.7769525573201292 1101 9 1.0910007435091416 1101 10 1.0385785054789949 1101 11 0.8166741773966706 1101 12 1.0109453707540013 1101 13 1.1223705456234612 1101 14 1.1359460342781489 1101 15 1.225752923432701 1101 16 1.2279226975408477 1101 17 1.1615536870161591 1101 18 1.319466180121978 1101 19 1.1031344216548717 1101 20 0.9954672342065933 1101 21 0.8753314226710955 1101 22 1.0010555238581844 1101 23 0.9091074592713966 1101 24 0.9479166527786305 1101 25 0.9450094084451095 1102 1 0.8578521087263589 1102 2 0.8235134727105056 1102 3 1.0897809495258066 1102 4 0.9110938384704852 1102 5 0.7904278008092334 1102 6 0.6654410368376418 1102 7 0.6629560939901182 1102 8 0.7113631713830912 1102 9 0.9477671595820638 1102 10 0.8951092285904991 1102 11 0.6431575901952229 1102 12 0.9369289833575891 1102 13 0.8041452474300996 1102 14 1.1235928892860159 1102 15 1.2067469560125943 1102 16 1.044533123573899 1102 17 1.1307313920227244 1102 18 1.1599648544045849 1102 19 0.8296230495170676 1102 20 0.23857958587969819 1102 21 0.4417254848918333 1102 22 0.48974137349990343 1102 23 0.6633592164898374 1102 24 0.8213940064599328 1102 25 0.8276995125298257 1103 1 0.690455216513854 1103 2 0.7760258111582914 1103 3 1.0011589014507116 1103 4 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0.34522152673618933 2207 6 0.37494857243629554 2207 7 0.33849208173896983 2207 8 0.3783694040478238 2207 9 -1.2305882076132733 2207 10 -0.22102725106053356 2207 11 0.16957546787649846 2207 12 0.4976515048381458 2207 13 0.629187994371776 2207 14 0.9041868486701787 2207 15 0.7403086597504114 2207 16 0.8238636386433171 2207 17 0.7924259379056267 2207 18 0.8160900270879523 2207 19 -0.29215821286526733 2207 20 0.38672094171630533 2207 21 -0.22795845546591664 2207 22 0.27219647582483475 2207 23 -0.464976428212573 2207 24 -1.4483092994225473 2207 25 -0.11170089691269425 2208 1 0.44298010068234106 2208 2 0.16988959282275573 2208 3 0.6501308441851954 2208 4 0.411394734656092 2208 5 0.365229454834072 2208 6 0.25335332149776946 2208 7 0.33937035995432296 2208 8 0.43714860324126903 2208 9 -0.27061766322631087 2208 10 -0.06868562500090292 2208 11 0.1615834546445143 2208 12 0.2059577180151777 2208 13 0.47958311275699117 2208 14 0.7270391416225266 2208 15 0.6864590681229785 2208 16 0.4906834847211812 2208 17 0.4507787066754645 2208 18 0.6773234805024231 2208 19 -1.2959523358456195 2208 20 -0.13546305527145108 2208 21 -0.23665008528398346 2208 22 0.10181307697111208 2208 23 0.27501804847850764 2208 24 0.3761128011602324 2208 25 0.5417114305337307 2209 1 0.19108339830178167 2209 2 -1.4264096270813234 2209 3 -0.16561740482584497 2209 4 0.12121510164377014 2209 5 0.1638047232741755 2209 6 0.27037070656453466 2209 7 0.2092569846153154 2209 8 0.4051660694660768 2209 9 -1.9224631150716647 2209 10 -0.24213425083551954 2209 11 0.07324575890091012 2209 12 0.40049281922693325 2209 13 0.4933080030568604 2209 14 0.626666562423253 2209 15 0.809409257324198 2209 16 0.7564209173930863 2209 17 0.47045158960875 2209 18 0.7099945797573 2209 19 0.49878983857987436 2209 20 0.4629113834635348 2209 21 0.4458350159116904 2209 22 0.3533384817944025 2209 23 0.424856784027547 2209 24 0.5670245849459761 2209 25 0.5344849574059864 2210 1 0.15336754156937715 2210 2 0.1931210605249909 2210 3 0.168847342902545 2210 4 0.2083725540078234 2210 5 0.22237555420526434 2210 6 0.16836463622676234 2210 7 0.11534167392742489 2210 8 0.19565711881497094 2210 9 0.3660411327353472 2210 10 -1.5209795906362515 2210 11 -0.3976461233326347 2210 12 -2.1611842712039433 2210 13 -0.6807260651653522 2210 14 -0.34354689047444964 2210 15 -0.6407962479099183 2210 16 -0.08532552926750725 2210 17 0.11888162110398781 2210 18 0.33311568633127564 2210 19 0.28572710063836126 2210 20 0.26557444159868737 2210 21 0.25569494072335885 2210 22 0.2680203703651074 2210 23 0.14312430772044848 2210 24 0.21093994328172982 2210 25 0.23526960335853886 2211 1 -0.2563588988670027 2211 2 -0.07759310486665782 2211 3 -1.4959060846906418 2211 4 -0.6091317295492047 2211 5 -0.4882131395964109 2211 6 -0.44532859231433974 2211 7 -0.4777614952568783 2211 8 -0.15924535363720338 2211 9 0.1554878636262913 2211 10 0.10869380246919036 2211 11 -0.20852868060870122 2211 12 -0.22321300766675733 2211 13 -0.24581009664289155 2211 14 -0.7901776274256775 2211 15 -0.2925086241253396 2211 16 -0.06909374372943944 2211 17 -0.13717776559231964 2211 18 -0.07036175459849403 2211 19 0.0013651587591780867 2211 20 -0.06209423685294979 2211 21 -0.20561591244955935 2211 22 -0.11925197814050392 2211 23 -0.41114414845470293 2211 24 -0.18742924501037805 2211 25 -0.20188202808112976 2212 1 -0.26530163543523955 2212 2 -0.24365618137014167 2212 3 -2.386996156439782 2212 4 -1.257441182355116 2212 5 -0.4980958047300774 2212 6 -0.2765449658159014 2212 7 -0.18528793946333622 2212 8 -0.3332400003143263 2212 9 -0.8646413524992269 2212 10 -0.13148411698190898 2212 11 -0.179290772959952 2212 12 0.07161319094755214 2212 13 -0.21925927126237355 2212 14 0.09453651808304642 2212 15 0.2931263270778288 2212 16 -1.0092970558452805 2212 17 -0.9966099838788622 2212 18 -0.10924100518085567 2212 19 -0.0033783077643931847 2212 20 -0.04479885940974526 2212 21 0.07169751983769146 2212 22 -0.1860541660390922 2212 23 0.07658418959701407 2212 24 -0.01427987463279834 2212 25 0.16974008539500574 2213 1 -3.0218048722237256 2213 2 -1.4390468555169882 2213 3 -2.3675544561979045 2213 4 -1.1443770711795835 2213 5 -0.8734925117617536 2213 6 -0.7061780924089192 2213 7 -0.47198666190465843 2213 8 -0.27137736394365675 2213 9 -0.3213771581192262 2213 10 -0.2245298155238089 2213 11 -0.5654602162861551 2213 12 -0.2687944661064918 2213 13 -0.6213437563197743 2213 14 -0.28712710036950284 2213 15 -0.49309010098338035 2213 16 -1.6007558141250797 2213 17 -1.1735081839934622 2213 18 -0.4395739688411382 2213 19 -0.292587741940352 2213 20 -0.27777788223939126 2213 21 -0.0990696769677939 2213 22 -0.1889931228882631 2213 23 -0.05633596214462244 2213 24 -0.006439367063094892 2213 25 -0.07852949709123891 2214 1 -0.8914916382439699 2214 2 -0.7196711907273219 2214 3 -6.3359299429572395 2214 4 -2.167279001769444 2214 5 -1.226847118364418 2214 6 -0.6504348067280716 2214 7 -0.5948594027494281 2214 8 -0.37536813528492985 2214 9 -0.3185790996439479 2214 10 -0.2166571695683075 2214 11 -0.6413337623341064 2214 12 -0.5043907728355848 2214 13 -5.284582480538134 2214 14 -3.7856234218290297 2214 15 -2.031146479665651 2214 16 -2.803685904233731 2214 17 -3.2660759145947083 2214 18 -1.096288649475504 2214 19 -0.599594094098812 2214 20 -0.3805486487548677 2214 21 -0.3777282681062246 2214 22 -0.22434569976438468 2214 23 -0.2008348700143756 2214 24 -0.1463824154304092 2214 25 -0.04830238641510931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1354.0 3 189.0 4 169.0 5 393.0 6 61.0 7 41.0 8 99.0 9 270.0 10 702.0 11 1645.0 12 3834.0 13 7323.0 14 12129.0 15 17697.0 16 22690.0 17 26201.0 18 27776.0 19 27804.0 20 27618.0 21 26719.0 22 25980.0 23 26121.0 24 26867.0 25 28585.0 26 30906.0 27 35406.0 28 40945.0 29 50023.0 30 63081.0 31 80055.0 32 106695.0 33 143517.0 34 197633.0 35 275903.0 36 396286.0 37 609435.0 38 1231060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55939795660238 21.105247779679928 13.130192571077837 22.20516169263985 2 14.615446433046595 21.868721643705356 41.37776835698627 22.13806356626178 3 18.43058279110363 28.615167235988014 28.862030274884347 24.092219698024003 4 11.44058043896929 18.078712739047187 38.570031746956204 31.910675075027317 5 12.04504263101291 40.30157406170752 34.169489741033146 13.483893566246419 6 29.43341093523028 39.23242174208557 18.11736845834352 13.216798864340626 7 25.10668029515955 34.849269802153486 22.93829373136 17.10575617132696 8 24.041119673820514 37.80450513015816 21.13457144137282 17.019803754648503 9 24.814932433605595 18.025079686730752 21.98438022458468 35.17560765507898 10 14.589466898282403 31.701997587693935 32.790251751890374 20.918283762133292 11 30.919211631468542 27.827338073087876 22.167383398425848 19.08606689701773 12 20.524160294815662 29.7201239413113 31.234134546050246 18.521581217822796 13 26.141414062213407 25.294459779262628 26.53491386661163 22.029212291912337 14 20.061025794543692 24.624826621718764 28.45775730765127 26.856390276086273 15 23.112693557235644 30.898306086265904 24.564731633398612 21.42426872309984 16 20.56211170210085 32.39565907241432 26.282527693659596 20.759701531825236 17 19.08299488057236 31.014081296432533 28.879889619079968 21.023034203915138 18 19.499848659798438 30.340403349663127 30.221601291436386 19.93814669910205 19 20.07758101379582 29.448214771310766 30.78632062618201 19.687883588711404 20 22.215124826726782 28.61832285526261 29.463336087035664 19.703216230974952 21 22.283826072346173 29.227406696956752 28.61612562219531 19.87264160850176 22 20.76077124494168 28.924037694106445 29.792234341963397 20.522956718988475 23 20.33455646890106 29.408909912046816 29.56590215792927 20.690631461122855 24 20.41391896047504 29.094868751459856 29.679819994320212 20.811392293744895 25 20.899514981414484 28.70658069878635 28.69462256402013 21.699281755779044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3541.0 1 3541.0 2 3823.0 3 4105.0 4 4105.0 5 4105.0 6 6953.5 7 9802.0 8 9802.0 9 9802.0 10 14963.0 11 20124.0 12 20124.0 13 20124.0 14 30831.5 15 41539.0 16 41539.0 17 41539.0 18 63405.0 19 85271.0 20 85271.0 21 85271.0 22 118644.5 23 152018.0 24 152018.0 25 152018.0 26 194028.5 27 236039.0 28 236039.0 29 236039.0 30 277328.0 31 318617.0 32 318617.0 33 318617.0 34 348357.5 35 378098.0 36 378098.0 37 378098.0 38 394587.5 39 411077.0 40 411077.0 41 411077.0 42 407087.5 43 403098.0 44 403098.0 45 403098.0 46 423627.5 47 444157.0 48 444157.0 49 444157.0 50 410617.5 51 377078.0 52 377078.0 53 377078.0 54 328147.5 55 279217.0 56 279217.0 57 279217.0 58 236808.5 59 194400.0 60 194400.0 61 194400.0 62 157666.5 63 120933.0 64 120933.0 65 120933.0 66 89957.0 67 58981.0 68 58981.0 69 58981.0 70 41189.5 71 23398.0 72 23398.0 73 23398.0 74 15822.0 75 8246.0 76 8246.0 77 8246.0 78 5368.5 79 2491.0 80 2491.0 81 2491.0 82 1606.5 83 722.0 84 722.0 85 722.0 86 451.0 87 180.0 88 180.0 89 180.0 90 115.5 91 51.0 92 51.0 93 51.0 94 33.5 95 16.0 96 16.0 97 16.0 98 14.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09988212286312706 2 0.0535932376808317 3 0.056000035822111875 4 0.051662201962827836 5 0.04900352959746021 6 0.06781013832932387 7 0.0633043883206482 8 0.0607856460797736 9 0.06587910261132 10 0.06590708863621862 11 0.0599180793079168 12 0.060645715955280566 13 0.061037520303861066 14 0.06333237434554681 15 0.06629889298479912 16 0.060953562229165245 17 0.06450778739128829 18 0.14250483878370498 19 0.06976916007222633 20 0.0633043883206482 21 0.0612054364532527 22 0.06610299081050887 23 0.06534736813824649 24 0.07438685418049643 25 0.06775416627952666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3573212.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.368199827817 #Duplication Level Percentage of deduplicated Percentage of total 1 89.35831752000554 69.1349216616531 2 8.193392615601875 12.67816074303292 3 1.3828608660665853 3.2096836745972293 4 0.39038731396027293 1.208142548668925 5 0.17669958375311945 0.6835464352651717 6 0.10511078847615535 0.4879339492129554 7 0.06350139354769958 0.3439091953740289 8 0.048890837622266384 0.30260768759270873 9 0.03305216543853087 0.23014678863512908 >10 0.2101392129508925 3.058909940172303 >50 0.02067476298143088 1.1070475175968602 >100 0.013871278422177056 2.195190669840471 >500 0.0012771546159089761 0.6545815419332166 >1k 0.0014960953772086675 2.1808468100552045 >5k 2.189407869085853E-4 1.1543118724462396 >10k+ 1.0947039345429265E-4 1.3700589639235836 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 17884 0.500502069286681 No Hit CCCATGTACTCTGCGTTGATACCAC 16219 0.4539053378305009 No Hit GTATCAACGCAGAGTACTTTTTTTT 14426 0.4037263951872993 No Hit GGTATCAACGCAGAGTACTTTTTTT 8561 0.23958835915697138 No Hit TATCAACGCAGAGTACTTTTTTTTT 8019 0.22441993366192658 No Hit GAGTACATGGGAAGCAGTGGTATCA 7705 0.21563232184376413 No Hit CATGTACTCTGCGTTGATACCACTG 5984 0.1674683729932621 No Hit GTATCAACGCAGAGTACATGGGAAG 5406 0.15129245060186747 No Hit GCGTTGATACCACTGCTTCCCATGT 5212 0.14586316177153777 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4368 0.12224295675711376 No Hit GGTATCAACGCAGAGTACATGGGAA 4307 0.12053580923829876 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3850 0.10774619585963553 No Hit ATTCCATTCCATTCCATTCCATTCC 3741 0.1046957191456874 No Hit TATCAACGCAGAGTACATGGGAAGC 3697 0.10346433405014872 No Hit GTGGTATCAACGCAGAGTACATGGG 3603 0.1008336477096797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.798602489860663E-5 0.0 4 0.0 0.0 0.0 5.597204979721326E-5 0.0 5 0.0 0.0 0.0 5.597204979721326E-5 0.0 6 0.0 2.798602489860663E-5 0.0 5.597204979721326E-5 0.0 7 0.0 2.798602489860663E-5 0.0 5.597204979721326E-5 0.0 8 0.0 2.798602489860663E-5 0.0 5.597204979721326E-5 0.0 9 2.798602489860663E-5 2.798602489860663E-5 0.0 8.39580746958199E-5 0.0 10 2.798602489860663E-5 2.798602489860663E-5 0.0 1.1194409959442652E-4 0.0 11 2.798602489860663E-5 2.798602489860663E-5 0.0 1.1194409959442652E-4 0.0 12 2.798602489860663E-5 2.798602489860663E-5 0.0 1.1194409959442652E-4 0.0 13 2.798602489860663E-5 2.798602489860663E-5 0.0 1.1194409959442652E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACTG 55 0.0030761133 12.087585 5 AGGACCG 75 0.0026550582 10.1305485 5 CGCCCTA 190 1.0459189E-9 9.504175 16 TCACGGT 100 2.7596598E-4 9.499118 1 GTCTTAG 340 0.0 9.499118 1 TAGGACC 230 3.6379788E-12 9.497389 4 GTCTAGG 345 0.0 9.361449 1 GTAGGAC 185 6.7411747E-9 9.240443 3 GTACTGG 645 0.0 9.130935 1 CACGGTA 95 0.0018297016 8.997273 2 CCGAGAC 95 0.0018297016 8.997273 3 TGCACTG 605 0.0 8.947952 5 TTAGACA 480 0.0 8.903801 4 TGTCCCG 205 4.1691237E-9 8.802211 11 ATTAGAC 315 0.0 8.743383 3 ACTGTCC 360 0.0 8.705696 8 CCGTGTG 120 1.7706845E-4 8.705695 9 GTATTAG 525 0.0 8.684907 1 GTACAAG 440 0.0 8.635562 1 TATTCCG 110 7.167626E-4 8.633989 5 >>END_MODULE