FastQCFastQC Report
Wed 25 May 2016
SRR1294671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573212
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT378641.0596628467608415No Hit
GGTATCAACGCAGAGTACTTTTTTT248830.6963762575520288No Hit
TATCAACGCAGAGTACTTTTTTTTT228500.6394806689331616No Hit
CCCATGTACTCTGCGTTGATACCAC179380.5020133146312058No Hit
GTACATGGGAAGCAGTGGTATCAAC175930.4923581360411865No Hit
ACGCAGAGTACTTTTTTTTTTTTTT137240.38408020570847745No Hit
GAGTACATGGGAAGCAGTGGTATCA87410.24462584363872056No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69120.19343940409916904No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67440.18873775191620312No Hit
CATGTACTCTGCGTTGATACCACTG66900.18722650657167836No Hit
GTATCAACGCAGAGTACATGGGAAG55360.1549306338386863No Hit
GCGTTGATACCACTGCTTCCCATGT54620.1528596679961894No Hit
ACGCAGAGTACATGGGAAGCAGTGG51620.14446386052660742No Hit
GGTATCAACGCAGAGTACATGGGAA47150.13195410739693028No Hit
ATCAACGCAGAGTACTTTTTTTTTT40670.11381916326263317No Hit
TATCAACGCAGAGTACATGGGAAGC40010.11197208561932513No Hit
GTGGTATCAACGCAGAGTACATGGG39990.11191611356952792No Hit
ACTCTGCGTTGATACCACTGCTTCC37650.10536738374325397No Hit
ATTCCATTCCATTCCATTCCATTCC35890.1004418433610992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCCG600.00579646211.1041999
TCGCGCG1105.9168487E-610.3831479
CGCCCTA2650.010.37804916
CTGTGCG951.6142467E-410.01882659
TATCGCG1054.021739E-59.9716787
GTCCCGC2700.09.83476412
GTTACAC2350.09.7040393
TGTCCCG2550.09.67610811
GCTCCGT800.00444624769.5184216
TGTATCG1304.1390995E-69.518425
CGCCTTT1401.0852218E-69.4833914
CGCGCGC1356.691529E-69.16537110
CTAGGAC3850.09.1316693
CTTAGAC2304.1836756E-119.0887473
GTCCTAC2201.6370905E-109.0790071
CAGGGCG1054.4113508E-49.0646539
CTTGGAC3150.09.0494013
TAGACTG4850.09.027785
ATCGAGA1901.0257281E-89.01694410
TTACACT4250.08.9585124
Produced by FastQC (version 0.11.5)