FastQCFastQC Report
Wed 25 May 2016
SRR1294670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4891639
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129340.26441035407559715No Hit
GTACATGGGAAGCAGTGGTATCAAC116780.23873388858008535No Hit
CCCATGTACTCTGCGTTGATACCAC110180.2252414783674756No Hit
GGTATCAACGCAGAGTACTTTTTTT79400.16231778346685027No Hit
ATTCCATTCCATTCCATTCCATTCC73910.1510945513354522No Hit
TATCAACGCAGAGTACTTTTTTTTT73880.15103322219812215No Hit
GAATGGAATGGAATGGAATGGAATG55510.11347934710635843No Hit
GAGTACATGGGAAGCAGTGGTATCA51850.10599719235209304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC701.0855781E-412.22179518
TAACGCC550.00307546812.08807119
AGCACCG802.8789216E-511.87111955
GTACCGC658.051559E-411.6883676
TAGACCG600.00589303311.0797125
AGTACCG600.00589303311.0797125
TATTCCG2500.010.2566475
GTACCGT750.00265672710.1299186
GTATTAG7350.09.9517761
AGCGTAC3650.09.6295511
CGCCCTA3100.09.50496616
TCCAGCG1106.866497E-59.4969922
ACCGTCC900.00111795879.4967018
TACACTG10150.09.3565475
ATAGGCG950.00183000958.9972443
AATACCG1401.0907581E-58.8185465
ACGGTGG4100.08.8018218
CTTACAC7250.08.7766213
TACACGG4450.08.7499495
AGGGCCG1107.172519E-48.6335415
Produced by FastQC (version 0.11.5)