##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294670_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4891639 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.390687456699073 31.0 30.0 34.0 26.0 34.0 2 30.501225458379082 31.0 30.0 34.0 26.0 34.0 3 30.50871844794761 31.0 30.0 34.0 26.0 34.0 4 34.17221651066238 37.0 35.0 37.0 30.0 37.0 5 34.542867124904355 37.0 35.0 37.0 31.0 37.0 6 34.59654667893522 37.0 35.0 37.0 31.0 37.0 7 34.54052762274567 37.0 35.0 37.0 30.0 37.0 8 34.36595668650119 37.0 35.0 37.0 30.0 37.0 9 35.59579049067194 39.0 35.0 39.0 29.0 39.0 10 35.715861289028076 39.0 35.0 39.0 30.0 39.0 11 35.933162075124514 39.0 35.0 39.0 30.0 39.0 12 35.73106662204631 39.0 35.0 39.0 30.0 39.0 13 35.60795512506135 39.0 35.0 39.0 29.0 39.0 14 36.570602000679116 40.0 36.0 41.0 28.0 41.0 15 36.56055383481896 40.0 36.0 41.0 28.0 41.0 16 36.68010946842153 40.0 36.0 41.0 29.0 41.0 17 36.679777473358115 40.0 36.0 41.0 29.0 41.0 18 36.56802760792446 39.0 36.0 41.0 28.0 41.0 19 36.858677633406714 40.0 36.0 41.0 29.0 41.0 20 36.952748352852694 40.0 36.0 41.0 30.0 41.0 21 37.00561979328401 40.0 36.0 41.0 30.0 41.0 22 37.00911657626411 40.0 36.0 41.0 30.0 41.0 23 36.99538171152859 40.0 36.0 41.0 30.0 41.0 24 36.996578856289275 40.0 36.0 41.0 30.0 41.0 25 35.95915091035949 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9413690267805741 1101 2 0.9464719343318144 1101 3 1.2336881736121015 1101 4 1.0175010062204777 1101 5 0.7596831066451273 1101 6 0.6967336709217591 1101 7 0.7692044285223574 1101 8 0.8918883468560139 1101 9 1.1276125594736612 1101 10 1.0325542764321014 1101 11 0.835923145812103 1101 12 1.0486258858111839 1101 13 1.095255070702244 1101 14 1.2655498463097103 1101 15 1.3892334559870534 1101 16 1.2774777434430575 1101 17 1.2765995683143458 1101 18 1.2575957242401046 1101 19 1.1071380948332035 1101 20 1.037230185352378 1101 21 0.9247487748749847 1101 22 0.9110772323794905 1101 23 0.9101311768958738 1101 24 0.9628952982926435 1101 25 0.9715508358904614 1102 1 0.7300157681162922 1102 2 0.7280647440390133 1102 3 1.028058986873301 1102 4 0.896011524498654 1102 5 0.6276265163355532 1102 6 0.5335415780027901 1102 7 0.5238425052988589 1102 8 0.5852406237314867 1102 9 0.8795516493112032 1102 10 0.7429149887484954 1102 11 0.5461272904248347 1102 12 0.8167046573977004 1102 13 0.8258186886441337 1102 14 0.9911522453784443 1102 15 1.0573236795805414 1102 16 0.9851517057315107 1102 17 0.9678998067655229 1102 18 1.0395958020159455 1102 19 0.7931288760401713 1102 20 0.09641860550095771 1102 21 0.3664690514376545 1102 22 0.5362103157649827 1102 23 0.6060376968102688 1102 24 0.692660591771336 1102 25 0.7738883248274178 1103 1 0.6768920616947653 1103 2 0.7014496048352079 1103 3 0.9593552878128939 1103 4 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0.3786080818427422 2207 9 -1.2322285962145827 2207 10 -0.10481642576787209 2207 11 0.10895062987361115 2207 12 0.4406910447734447 2207 13 0.4909853356541163 2207 14 0.7930651898651675 2207 15 0.8924751232144033 2207 16 0.7568367599870598 2207 17 0.7975759936044398 2207 18 0.8351629320151659 2207 19 -0.33267737079334836 2207 20 0.2476360434723759 2207 21 -0.2977242962229809 2207 22 0.263270475831213 2207 23 -0.5107558360178643 2207 24 -1.5475640883296649 2207 25 -0.17147367702246896 2208 1 0.4160733570035049 2208 2 0.14836529471943294 2208 3 0.648293005688803 2208 4 0.4786112669961682 2208 5 0.376668634079266 2208 6 0.27022175836602713 2208 7 0.31887550208585935 2208 8 0.553903119268341 2208 9 -0.2579865109111239 2208 10 0.04702360326042765 2208 11 0.20979875239736856 2208 12 0.19218052690133192 2208 13 0.4818494142767449 2208 14 0.8026446788655122 2208 15 0.7147558730578183 2208 16 0.5687942607704812 2208 17 0.5125062990636664 2208 18 0.6941255890801017 2208 19 -1.3308728659027835 2208 20 -0.09018712367503667 2208 21 -0.27012778993837827 2208 22 0.13376065565550022 2208 23 0.2717851521857213 2208 24 0.4614308962846039 2208 25 0.38500941510470454 2209 1 0.13650696686371333 2209 2 -1.4125384630191604 2209 3 -0.1882586837980078 2209 4 0.13431170845885276 2209 5 0.12250233708041236 2209 6 0.18198194305974624 2209 7 0.174380574746948 2209 8 0.29844539478685306 2209 9 -2.004633462643099 2209 10 -0.3289171360977221 2209 11 -0.021329129949926084 2209 12 0.2793964672436502 2209 13 0.42088930330169916 2209 14 0.5179777938262191 2209 15 0.704496094563801 2209 16 0.6278466197525674 2209 17 0.4810007466786388 2209 18 0.6658315571917655 2209 19 0.40512427581030863 2209 20 0.34288486858224587 2209 21 0.3617460181067642 2209 22 0.2700721045517511 2209 23 0.3355137143943452 2209 24 0.48820017762069057 2209 25 0.47999511292549357 2210 1 0.19283105603591721 2210 2 0.2340279620956629 2210 3 0.1651304930044759 2210 4 0.3403091853833402 2210 5 0.21820801822870095 2210 6 0.19237080704355947 2210 7 0.21142339559955303 2210 8 0.28637010155014053 2210 9 0.46882063019097586 2210 10 -1.2962689864899346 2210 11 -0.2867987725812853 2210 12 -2.176118152308092 2210 13 -0.5694707501648395 2210 14 -0.2700052482819686 2210 15 -0.5868683244063106 2210 16 0.03228029070017158 2210 17 0.2152862885446183 2210 18 0.4384836929797018 2210 19 0.27724152041151484 2210 20 0.33098189245335163 2210 21 0.2751721789431798 2210 22 0.28582457713290665 2210 23 0.21916655792425388 2210 24 0.28085556885749696 2210 25 0.2638480865091495 2211 1 -0.2875875012858238 2211 2 -0.07507851066559112 2211 3 -1.6511738786280965 2211 4 -0.588480295059064 2211 5 -0.4653524943288616 2211 6 -0.40292035598523057 2211 7 -0.4098656596870214 2211 8 -0.03832337827729049 2211 9 0.10027486066321956 2211 10 0.12701281495112937 2211 11 -0.14011048953229022 2211 12 -0.15268932854677786 2211 13 -0.22701823234050522 2211 14 -0.8095083012443567 2211 15 -0.28522029803119153 2211 16 -0.030584849442355733 2211 17 -0.07554870461424201 2211 18 -0.038329532200862104 2211 19 -0.03613305518419452 2211 20 -0.05646921462665233 2211 21 -0.15432345741202624 2211 22 -0.18346038377423213 2211 23 -0.49851273114649786 2211 24 -0.2804219578349887 2211 25 -0.14769442177280467 2212 1 -0.388551821913552 2212 2 -0.37859325156452783 2212 3 -2.596448199270263 2212 4 -1.3397443720868836 2212 5 -0.5952364414271045 2212 6 -0.39220941102841067 2212 7 -0.337732536019999 2212 8 -0.32274970555022975 2212 9 -0.9027516696337017 2212 10 -0.1843536693405028 2212 11 -0.2959429239862956 2212 12 -0.05144683906258507 2212 13 -0.3823291579479502 2212 14 -0.08746246634069621 2212 15 0.10650879923404943 2212 16 -1.0812865529089208 2212 17 -1.31471991621693 2212 18 -0.29674117994413507 2212 19 -0.165409778613828 2212 20 -0.2547284644197987 2212 21 -0.11506196800146995 2212 22 -0.3522899923537963 2212 23 -0.03742936857251067 2212 24 -0.16893036686911955 2212 25 0.10475773546537681 2213 1 -2.950927639436845 2213 2 -1.2707578074400665 2213 3 -2.3885243938718013 2213 4 -0.9650438519545617 2213 5 -0.6995010834734927 2213 6 -0.6051994104715774 2213 7 -0.326785447262786 2213 8 -0.17061920577464917 2213 9 -0.27690448646080057 2213 10 -0.1929493059142331 2213 11 -0.5502540416878077 2213 12 -0.25542071523435794 2213 13 -0.5514322704047601 2213 14 -0.226733542744185 2213 15 -0.31266049649995864 2213 16 -1.7126523747976705 2213 17 -1.0297330571953438 2213 18 -0.3503685668322021 2213 19 -0.1700974361848182 2213 20 -0.15983836225457537 2213 21 0.029775850205403742 2213 22 -0.020885253602848763 2213 23 0.027884570524598473 2213 24 0.07111512740425496 2213 25 0.0010456697921767955 2214 1 -0.9516701576730711 2214 2 -0.731668662461221 2214 3 -6.505629018699672 2214 4 -2.0125988132549395 2214 5 -1.2468582547226106 2214 6 -0.6529689944706121 2214 7 -0.6228949661893353 2214 8 -0.2951563595260609 2214 9 -0.0977883112919713 2214 10 -0.04911185927991113 2214 11 -0.7315399528392703 2214 12 -0.40081130225296135 2214 13 -5.078983009365718 2214 14 -3.7791034402996146 2214 15 -2.0574905537825146 2214 16 -2.635498788361126 2214 17 -3.276888803778675 2214 18 -1.1175184310110353 2214 19 -0.7115992949937038 2214 20 -0.3540409200952155 2214 21 -0.35918984676673205 2214 22 -0.3901067965044476 2214 23 -0.19583144389631002 2214 24 -0.13876128023554912 2214 25 0.01229098668158457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1836.0 3 249.0 4 239.0 5 563.0 6 79.0 7 85.0 8 187.0 9 439.0 10 940.0 11 2099.0 12 4165.0 13 7474.0 14 12187.0 15 17017.0 16 22103.0 17 25968.0 18 27411.0 19 27725.0 20 28226.0 21 28392.0 22 29346.0 23 29952.0 24 32305.0 25 35165.0 26 39650.0 27 45300.0 28 53744.0 29 66588.0 30 83417.0 31 106837.0 32 143593.0 33 194730.0 34 270182.0 35 381266.0 36 549140.0 37 854730.0 38 1768310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60867280411171 22.53237547281164 13.413546580490324 22.44540514258632 2 13.881498841398274 22.608922777543356 42.17657994351906 21.33299843753931 3 16.954922390224805 29.337407285742163 30.8749165975652 22.832753726467832 4 11.133512150762973 18.230828293951635 40.59093610801696 30.044723447268424 5 12.077288757636316 39.50910365305798 35.7113215161022 12.702286073203512 6 28.369805563493962 40.94376996975041 18.438031303526706 12.248393163228922 7 23.628083736779455 34.88321285692631 25.528356396602724 15.960347009691503 8 22.497886425193773 39.07449049704447 22.658135590758732 15.769487487003023 9 23.84167668698278 17.578382465271883 23.44140273242667 35.13853811531867 10 14.128697745512628 31.16150449719171 34.37360894725128 20.336188810044384 11 30.834329168485493 27.931467995337815 23.922299084377137 17.31190375179956 12 19.219302331337488 29.180573871780226 33.51688082586348 18.083242971018805 13 25.030729587606785 25.780676760740423 28.227199914249674 20.96139373740312 14 19.046715084786257 24.030751179087037 29.953454291353143 26.96907944477356 15 21.88384877953582 31.635267755042257 25.269853894516515 21.211029570905406 16 20.37499713620847 32.927772722958444 26.952185318404524 19.74504482242856 17 18.49485514188173 31.692330099808263 30.331230222607346 19.481584535702662 18 18.947036763613003 31.28774394735409 30.829503918266504 18.935715370766403 19 19.6361720077803 29.93919697952884 31.050107155043815 19.37452385764704 20 21.261012544502258 29.409129478148376 29.791004059309618 19.53885391803975 21 21.539543486523186 30.364532471140105 29.020696646782383 19.075227395554325 22 19.874780499699696 29.85838241575525 30.737836042072047 19.529001042473002 23 19.73864308872556 30.352112373529966 30.31743852961627 19.591806008128206 24 19.790985561874116 29.172371238971728 30.39852353529906 20.638119663855093 25 20.53405980949435 29.20513132091745 29.504890703306337 20.755918166281866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4221.0 1 4221.0 2 4357.0 3 4493.0 4 4493.0 5 4493.0 6 7802.5 7 11112.0 8 11112.0 9 11112.0 10 18591.0 11 26070.0 12 26070.0 13 26070.0 14 43842.0 15 61614.0 16 61614.0 17 61614.0 18 96337.0 19 131060.0 20 131060.0 21 131060.0 22 183053.5 23 235047.0 24 235047.0 25 235047.0 26 301774.5 27 368502.0 28 368502.0 29 368502.0 30 435275.5 31 502049.0 32 502049.0 33 502049.0 34 551640.5 35 601232.0 36 601232.0 37 601232.0 38 624315.5 39 647399.0 40 647399.0 41 647399.0 42 620072.5 43 592746.0 44 592746.0 45 592746.0 46 572772.5 47 552799.0 48 552799.0 49 552799.0 50 493551.5 51 434304.0 52 434304.0 53 434304.0 54 368792.5 55 303281.0 56 303281.0 57 303281.0 58 250197.0 59 197113.0 60 197113.0 61 197113.0 62 158324.0 63 119535.0 64 119535.0 65 119535.0 66 88686.5 67 57838.0 68 57838.0 69 57838.0 70 41696.0 71 25554.0 72 25554.0 73 25554.0 74 17893.0 75 10232.0 76 10232.0 77 10232.0 78 6978.5 79 3725.0 80 3725.0 81 3725.0 82 2471.5 83 1218.0 84 1218.0 85 1218.0 86 786.5 87 355.0 88 355.0 89 355.0 90 227.0 91 99.0 92 99.0 93 99.0 94 60.5 95 22.0 96 22.0 97 22.0 98 20.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10047756999238906 2 0.05225242500519764 3 0.05505312227660299 4 0.05071919657194654 5 0.047795841025881095 6 0.06762559542926205 7 0.06398673328101277 8 0.06137002342159755 9 0.06617413917911767 10 0.06654211400309794 11 0.060204769812326704 12 0.061042934689170644 13 0.06190154261179127 14 0.06359831541125582 15 0.06650122791154459 16 0.06163578301669441 17 0.06472268292897329 18 0.14475720714468093 19 0.06940414041183333 20 0.06384363196057599 21 0.06175844129135449 22 0.06670565836931139 23 0.06599015176712754 24 0.07660009252522519 25 0.06919970995406652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4891639.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.19125741387535 #Duplication Level Percentage of deduplicated Percentage of total 1 85.59873348144144 63.506776700233324 2 10.519649547286656 15.609320549330036 3 2.1822431382565495 4.857100872301645 4 0.6811226068519052 2.0213337062143806 5 0.2919659633590405 1.083066097183034 6 0.15421368336218688 0.6864784247439524 7 0.10897458885383975 0.565947324125852 8 0.07458135792756482 0.4426627779424268 9 0.06048355867081022 0.40386161435879825 >10 0.28934401174051577 3.9728757974544275 >50 0.02141708757784891 1.1061860328174422 >100 0.014354568090049006 2.073860880352453 >500 0.001694294897536688 0.9044141204251411 >1k 9.999095596984922E-4 1.3424930285362515 >5k 1.3887632773406657E-4 0.6894011215898289 >10k+ 8.332579664043994E-5 0.7342209523911405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12934 0.26441035407559715 No Hit GTACATGGGAAGCAGTGGTATCAAC 11678 0.23873388858008535 No Hit CCCATGTACTCTGCGTTGATACCAC 11018 0.2252414783674756 No Hit GGTATCAACGCAGAGTACTTTTTTT 7940 0.16231778346685027 No Hit ATTCCATTCCATTCCATTCCATTCC 7391 0.1510945513354522 No Hit TATCAACGCAGAGTACTTTTTTTTT 7388 0.15103322219812215 No Hit GAATGGAATGGAATGGAATGGAATG 5551 0.11347934710635843 No Hit GAGTACATGGGAAGCAGTGGTATCA 5185 0.10599719235209304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0443045776681393E-5 0.0 5 0.0 0.0 0.0 2.0443045776681393E-5 0.0 6 0.0 0.0 0.0 2.0443045776681393E-5 0.0 7 0.0 0.0 0.0 2.0443045776681393E-5 0.0 8 0.0 0.0 0.0 2.0443045776681393E-5 0.0 9 0.0 0.0 0.0 4.0886091553362786E-5 0.0 10 0.0 0.0 0.0 4.0886091553362786E-5 0.0 11 0.0 6.132913733004418E-5 0.0 4.0886091553362786E-5 0.0 12 0.0 6.132913733004418E-5 0.0 8.177218310672557E-5 0.0 13 0.0 6.132913733004418E-5 0.0 8.177218310672557E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCC 70 1.0855781E-4 12.221795 18 TAACGCC 55 0.003075468 12.088071 19 AGCACCG 80 2.8789216E-5 11.8711195 5 GTACCGC 65 8.051559E-4 11.688367 6 TAGACCG 60 0.005893033 11.079712 5 AGTACCG 60 0.005893033 11.079712 5 TATTCCG 250 0.0 10.256647 5 GTACCGT 75 0.002656727 10.129918 6 GTATTAG 735 0.0 9.951776 1 AGCGTAC 365 0.0 9.629551 1 CGCCCTA 310 0.0 9.504966 16 TCCAGCG 110 6.866497E-5 9.496992 2 ACCGTCC 90 0.0011179587 9.496701 8 TACACTG 1015 0.0 9.356547 5 ATAGGCG 95 0.0018300095 8.997244 3 AATACCG 140 1.0907581E-5 8.818546 5 ACGGTGG 410 0.0 8.801821 8 CTTACAC 725 0.0 8.776621 3 TACACGG 445 0.0 8.749949 5 AGGGCCG 110 7.172519E-4 8.633541 5 >>END_MODULE