FastQCFastQC Report
Wed 25 May 2016
SRR1294670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4891639
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT328720.6720038007710708No Hit
GGTATCAACGCAGAGTACTTTTTTT215110.4397503577021935No Hit
TATCAACGCAGAGTACTTTTTTTTT202390.41374680347425474No Hit
CCCATGTACTCTGCGTTGATACCAC122310.25003889289459014No Hit
ACGCAGAGTACTTTTTTTTTTTTTT118180.24159591498882074No Hit
GTACATGGGAAGCAGTGGTATCAAC115040.23517679861494276No Hit
ATTCCATTCCATTCCATTCCATTCC72080.1473534739583195No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71800.14678106867657242No Hit
GAGTACTTTTTTTTTTTTTTTTTTT64020.13087637906231428No Hit
GAATGGAATGGAATGGAATGGAATG60360.12339422430804889No Hit
GAGTACATGGGAAGCAGTGGTATCA55380.11321358751126157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT657.869644E-411.720254
TCGCGCG2500.011.0446369
TATCGCG2550.010.82974157
TGTATCG3200.010.7130415
CTGCGCA1201.482651E-610.3146749
GCGCGCC2950.010.2911511
GTATCGC3100.010.1370736
AGGGACG856.4551074E-410.0828627
ATCGCGC2950.010.0069098
CGCGCGC2950.010.00536810
GCGCCTT2850.09.98035213
CGCCTTT3650.09.87097914
CGCCCTA3750.09.8606916
CGCGCCT2850.09.64787112
GCACCGT800.00443027089.5227046
TACGACA1002.6922286E-49.5227044
TTACGCT800.00443027089.5227044
TAGCGTT1002.6963957E-49.5212379
TATACTG13250.09.4148995
TAGGACT5300.09.343034
Produced by FastQC (version 0.11.5)