FastQCFastQC Report
Wed 25 May 2016
SRR1294669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253888
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125590.5572149104125848No Hit
GGTATCAACGCAGAGTACTTTTTTT76770.3406114234602607No Hit
TATCAACGCAGAGTACTTTTTTTTT69610.30884409518130446No Hit
ATTCCATTCCATTCCATTCCATTCC50140.22246003350654514No Hit
GTACATGGGAAGCAGTGGTATCAAC45810.20324878609762329No Hit
CCCATGTACTCTGCGTTGATACCAC43680.1937984496124031No Hit
GAATGGAATGGAATGGAATGGAATG39300.1743653633188517No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31010.13758447624726694No Hit
GTACTGGTTCACTATCGGTCAGTCA24010.10652703239912543No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23450.1040424368912741No Hit
GTTAATGATAGTGTGTCGAAACACA22700.10071485362183037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA350.002174660616.28116610
ATCGCCG1155.347829E-912.38839319
AATCGCC1209.938958E-911.88170818
GTGTAGC1201.00335455E-811.8740561
AGCGTCG2150.011.49409617
TCGCACG600.005889278411.0802379
AAATCGC1303.2443495E-810.96699917
TATTCCG1451.4515535E-810.4793655
TAGGACC1106.071359E-610.3602824
CTAGACT1409.739233E-810.1752774
GGACCTT750.002654992710.1302796
CGCGCCT2350.010.11096912
TCGCGCG2350.010.103569
CTAGATA1606.208211E-910.0904834
GTATCGC3150.09.9493816
TGTATCG3150.09.949165
GCGATAA2201.8189894E-129.92838311
GTGTAGG1752.577508E-99.7706521
GCGTCGG2550.09.69174618
TATCGCG2450.09.6909557
Produced by FastQC (version 0.11.5)