FastQCFastQC Report
Wed 25 May 2016
SRR1294669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253888
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT327591.4534440043160974No Hit
GGTATCAACGCAGAGTACTTTTTTT214510.951733182837834No Hit
TATCAACGCAGAGTACTTTTTTTTT205910.9135768946815458No Hit
ACGCAGAGTACTTTTTTTTTTTTTT115300.5115604679558168No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71520.31731834057415453No Hit
GAGTACTTTTTTTTTTTTTTTTTTT63470.2816022801487918No Hit
CCCATGTACTCTGCGTTGATACCAC49360.21899934690632364No Hit
ATTCCATTCCATTCCATTCCATTCC48350.21451820143680608No Hit
GTACATGGGAAGCAGTGGTATCAAC44280.19646051622795807No Hit
GAATGGAATGGAATGGAATGGAATG42980.19069270522758894No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40800.18102052985773917No Hit
ATCAACGCAGAGTACTTTTTTTTTT34920.15493227702530027No Hit
GTACTGGTTCACTATCGGTCAGTCA23760.10541783797597751No Hit
GTTAATGATAGTGTGTCGAAACACA22660.10053738251412671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGCG400.005203380714.2814617
GCTATAC655.459036E-513.1527083
AATCGCC957.574636E-812.97580218
CAGCGTA1207.166818E-1012.69406610
CGCAGCG1155.156835E-912.4186618
GGTATCA51850.012.3612021
GGGCGAG701.06908104E-412.2412517
CTGAGCG550.003019907512.1170639
ATCGCCG1052.788347E-711.74001219
AAATCGC1201.3093813E-711.06270417
CGCCTTT3450.010.71890414
GCGCCTT3250.010.50302313
GCGTACT1002.4501262E-510.43031812
GGACAGT1855.820766E-1110.2929446
AGCGTCG1951.6370905E-1110.21172617
CGCGCCT3450.010.16921612
GCTAGGC750.002650260710.1324563
GCAGCGT1605.9608283E-910.1155849
CTTACAC3300.010.0748853
CCGTACA4350.010.02727817
Produced by FastQC (version 0.11.5)