FastQCFastQC Report
Wed 25 May 2016
SRR1294668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301671
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119680.5199700565371854No Hit
GGTATCAACGCAGAGTACTTTTTTT78580.34140413638612993No Hit
TATCAACGCAGAGTACTTTTTTTTT70850.3078198404550433No Hit
GTACTGGTTCACTATCGGTCAGTCA34380.1493697405059194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29550.12838498638597784No Hit
GTTAATGATAGTGTGTCGAAACACA24940.10835605957584729No Hit
GGTATCAACGCAGAGTACATGGGGG23280.10114390805636427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC456.778296E-414.7736846
AATGCGA400.00528983414.24605410
TCGACTT604.0796518E-412.67170113
GGTATCA27400.012.2748611
AATCGCC1405.2750693E-1112.22020418
CCGAATG2300.011.98406618
ATCGCCG1353.7289283E-1011.96120919
TACACCG752.0799843E-411.3968425
GTATAGA2450.011.2459411
GTCCTAC1053.4641143E-610.858151
GTACTAG1408.345523E-910.858151
AAATCGC1909.094947E-1210.50440317
GCTAGAC1002.4065166E-510.4473313
CGAATGG2650.010.39470319
GTACAAG3300.010.3645971
CCCCCGG3600.010.29665418
CCCCGGT3800.09.99853519
GTCTAGT1252.5679547E-69.8809171
GTAGGAC1252.5794034E-69.8774783
GTCCTAG1451.6156628E-79.8284981
Produced by FastQC (version 0.11.5)