FastQCFastQC Report
Wed 25 May 2016
SRR1294668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301671
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT314101.3646607182347088No Hit
GGTATCAACGCAGAGTACTTTTTTT218470.949179965338226No Hit
TATCAACGCAGAGTACTTTTTTTTT204730.8894842051709388No Hit
ACGCAGAGTACTTTTTTTTTTTTTT109770.4769143808997897No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66920.29074528896614676No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57220.24860199394266166No Hit
GCAGAGTACTTTTTTTTTTTTTTTT39660.17230959594138345No Hit
ATCAACGCAGAGTACTTTTTTTTTT31100.13511922425055536No Hit
GTACTGGTTCACTATCGGTCAGTCA30880.13416339694074436No Hit
GGTATCAACGCAGAGTACATGGGGG24790.10770435913733979No Hit
GTTAATGATAGTGTGTCGAAACACA24240.10531479086281227No Hit
GTGGTATCAACGCAGAGTACTTTTT23250.10101356796866276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.014.3031311
CGCAGAA1201.2781129E-711.0821022
TCTAGAC1752.0008883E-1110.8559373
CGATAGT803.848034E-410.66448818
TAGCACG1002.3315017E-510.4773514
CTAGTAC1002.4026884E-510.4488393
GTATCAA91500.010.3723391
TGTCCCG4950.010.15709911
TACAGGG8350.010.1246222
ACGGTAC2350.010.105263
ATACAGG8450.010.0161441
GTGCTAG1909.822543E-1110.0102211
CTAGACC1053.990253E-59.978434
TAGACAG2105.456968E-129.978435
ACAGGGT7800.09.8642883
CCGTACA7250.09.80642617
CCCTATA1651.0244548E-89.7867912
CCCCCGG4750.09.77889918
GTCTTAG2054.0017767E-119.7416541
CCCCGGT4750.09.579329519
Produced by FastQC (version 0.11.5)