##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294668_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2301671 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33076230269226 31.0 31.0 34.0 28.0 34.0 2 31.46543837064463 31.0 31.0 34.0 28.0 34.0 3 31.52814672470566 31.0 31.0 34.0 28.0 34.0 4 35.126938211412494 37.0 35.0 37.0 32.0 37.0 5 35.34617805933168 37.0 35.0 37.0 32.0 37.0 6 34.90701755376855 37.0 35.0 37.0 32.0 37.0 7 34.75490980248698 37.0 35.0 37.0 32.0 37.0 8 34.79630624880793 37.0 35.0 37.0 32.0 37.0 9 36.187811377038685 39.0 37.0 39.0 31.0 39.0 10 35.97574414414571 39.0 35.0 39.0 30.0 39.0 11 36.15716103648175 39.0 37.0 39.0 31.0 39.0 12 36.113862928281236 39.0 35.0 39.0 30.0 39.0 13 35.968533296027104 39.0 35.0 39.0 30.0 39.0 14 37.00985762083287 40.0 36.0 41.0 30.0 41.0 15 37.06865403439501 40.0 36.0 41.0 31.0 41.0 16 37.12184495525207 40.0 36.0 41.0 31.0 41.0 17 37.215706762608555 40.0 37.0 41.0 31.0 41.0 18 37.23639564472941 40.0 37.0 41.0 31.0 41.0 19 37.276113310720774 40.0 37.0 41.0 31.0 41.0 20 37.32920604204511 40.0 37.0 41.0 31.0 41.0 21 37.15374308491526 40.0 37.0 41.0 30.0 41.0 22 37.16146790744637 40.0 37.0 41.0 30.0 41.0 23 37.17555158838948 40.0 36.0 41.0 30.0 41.0 24 37.084112803263366 40.0 36.0 41.0 30.0 41.0 25 36.04779875142885 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4662774177820772 1101 2 0.40063002259049085 1101 3 0.4291250337695196 1101 4 0.3125850593718553 1101 5 0.3121585440928598 1101 6 0.3148224949377294 1101 7 0.43468977303500367 1101 8 0.3675032247504362 1101 9 0.5803043252272104 1101 10 0.6707615528964013 1101 11 0.6247133359767929 1101 12 0.7586167592249424 1101 13 0.6737544181807706 1101 14 0.8067452075803274 1101 15 0.8133757195202804 1101 16 0.8239971190374362 1101 17 0.9044170289889522 1101 18 0.966380625298406 1101 19 1.0144278066070243 1101 20 0.9612496410838389 1101 21 1.1553734946325136 1101 22 1.1131339997283476 1101 23 1.0893639328016107 1101 24 1.2124290572219465 1101 25 1.107909363704259 1102 1 0.3085656626415485 1102 2 0.34667708356480986 1102 3 0.2576656720899102 1102 4 0.21493281601340897 1102 5 0.24224663307668237 1102 6 0.3287661487783282 1102 7 0.3348052364763916 1102 8 0.3976762274734469 1102 9 0.4134327109520797 1102 10 0.5967194183622482 1102 11 0.5296106697334935 1102 12 0.5659140487206571 1102 13 0.6855665687835852 1102 14 0.8380350785499289 1102 15 0.8480491504202234 1102 16 0.7793149321598563 1102 17 0.676083743765389 1102 18 0.7498376007335565 1102 19 0.7910568033259366 1102 20 0.7868031439559928 1102 21 1.0194852921096356 1102 22 0.9848428105511218 1102 23 0.91422898490449 1102 24 0.9671207217236741 1102 25 1.024667198379852 1103 1 0.20691130943366076 1103 2 0.09732994751523805 1103 3 0.11017865256139814 1103 4 0.05193108097187604 1103 5 0.10932151151867231 1103 6 0.07933614145331802 1103 7 0.12823989106082934 1103 8 0.18582005643360588 1103 9 0.022115356817650422 1103 10 0.2927403035406613 1103 11 0.3169371049427028 1103 12 0.3761137644519721 1103 13 0.4019434689612922 1103 14 0.5386919041515768 1103 15 0.46984284056088654 1103 16 0.41838465560426386 1103 17 0.42079979039321813 1103 18 0.39785257092415094 1103 19 0.504654079650301 1103 20 0.43080027216311123 1103 21 0.7014972934873214 1103 22 0.602732252993178 1103 23 0.5749741957532706 1103 24 0.6613297578458486 1103 25 0.5525588399425914 1104 1 0.22896000136736205 1104 2 0.18329824312709775 1104 3 0.1251120815017117 1104 4 0.11587463838834111 1104 5 0.11726340130930168 1104 6 0.10118479181892326 1104 7 0.18904289055908663 1104 8 0.08692153288075843 1104 9 -0.1116939020152472 1104 10 0.2879123440242566 1104 11 0.42866636486299114 1104 12 0.43552427186511977 1104 13 -0.09551996941735297 1104 14 0.2888604855482484 1104 15 0.47146317169178786 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0.049380963632550845 2207 1 0.1771156427528986 2207 2 0.2243027128260202 2207 3 0.2444866417006466 2207 4 0.2994997317003296 2207 5 0.17528941459617897 2207 6 0.27517934401976163 2207 7 0.34029057234938165 2207 8 0.32653341006562187 2207 9 0.39521610348637637 2207 10 0.6080551028255528 2207 11 0.5079722001407205 2207 12 0.4077155925532523 2207 13 0.4328753661607365 2207 14 0.47752404383715685 2207 15 0.3960874237050547 2207 16 0.4826163422037766 2207 17 0.5135736145039687 2207 18 0.5612890874364851 2207 19 0.5595777641865141 2207 20 0.6023288446979791 2207 21 0.8974116817048099 2207 22 0.7534525411518658 2207 23 0.6764116806385942 2207 24 0.9159542662021778 2207 25 0.7605197323966308 2208 1 0.20082288849225804 2208 2 0.14828724499982116 2208 3 0.13604390757111773 2208 4 0.06782517877783789 2208 5 0.16094173479308438 2208 6 0.20504932028241996 2208 7 0.25575170808969716 2208 8 0.16722719778158535 2208 9 0.24635959756196257 2208 10 0.2782601460897567 2208 11 -0.003670530827385221 2208 12 -0.266355993702156 2208 13 0.07597743416502567 2208 14 -0.29134587608962903 2208 15 0.15591488522641583 2208 16 0.27467118338027774 2208 17 0.2565106083025199 2208 18 0.34319258179512957 2208 19 0.4976620707687047 2208 20 0.31592316780688634 2208 21 0.6086720639781475 2208 22 0.680148776950837 2208 23 0.6026335187925369 2208 24 0.6905486640273395 2208 25 0.6214760526148595 2209 1 -0.01381760514303565 2209 2 0.020322776261828324 2209 3 0.07475306553242689 2209 4 -0.5590754330196717 2209 5 -0.16406288258001211 2209 6 0.0575520782987482 2209 7 0.19205571097108276 2209 8 0.13884019066179576 2209 9 0.23641602700141107 2209 10 0.1744058980356158 2209 11 0.16750355459596733 2209 12 0.2170467213486731 2209 13 0.3486663724634482 2209 14 0.3180885692654911 2209 15 -0.25681499082592296 2209 16 -0.3137960898136498 2209 17 0.11853964510962811 2209 18 -0.23161674994247505 2209 19 0.18917945123457258 2209 20 0.22034576042731402 2209 21 0.5756480975961651 2209 22 0.5311900167394441 2209 23 0.47202806330862046 2209 24 0.5280152257381303 2209 25 0.5202669123785881 2210 1 -0.10503449400242815 2210 2 -0.11748640277269118 2210 3 -0.05376363210572066 2210 4 -0.0465989554449564 2210 5 -0.027134455046365247 2210 6 -0.5357317075777033 2210 7 -0.1584280801594602 2210 8 -0.233833837262317 2210 9 -0.02502026161960913 2210 10 0.21795392664552082 2210 11 0.07107267215762647 2210 12 0.04189971051141583 2210 13 0.16862493428058656 2210 14 0.21562213668238428 2210 15 0.01587473023717223 2210 16 0.11434834513656256 2210 17 -0.9534682712839668 2210 18 -1.3408888717378247 2210 19 -0.27888310977509434 2210 20 -0.07315966511254146 2210 21 -0.285362022058834 2210 22 -0.17814470477992472 2210 23 -0.06958346997327425 2210 24 0.3490094828788557 2210 25 0.4123502249720943 2211 1 -0.1997116222984019 2211 2 -0.16486290905561773 2211 3 -0.1427789213318711 2211 4 -0.06271964756943049 2211 5 -0.08372089679240702 2211 6 -0.11172698353953336 2211 7 -0.07276932581605422 2211 8 -0.07685374035784065 2211 9 -0.08464828768114074 2211 10 -0.021374572480844733 2211 11 -0.36865856003633724 2211 12 -0.3422314432423619 2211 13 -0.17300340466033504 2211 14 -0.21866406864140941 2211 15 -0.2274558048086277 2211 16 -0.2862816441329983 2211 17 -0.2945235121239804 2211 18 -0.33161217398152587 2211 19 -0.2110375928740993 2211 20 -0.3160929755307649 2211 21 0.1464723986405616 2211 22 -0.07511691359827921 2211 23 -0.6346642347210221 2211 24 -0.11272316388251369 2211 25 -0.6777582912965343 2212 1 -0.1996552081243408 2212 2 -0.2678830261933811 2212 3 -0.17507995363290618 2212 4 -0.0587502836000624 2212 5 -0.075576100412313 2212 6 -0.07329247686973162 2212 7 -0.1573388739150161 2212 8 -0.2408121349044663 2212 9 -0.04441636274333405 2212 10 -0.347225192449109 2212 11 -0.06263282106942114 2212 12 -0.1918923705503488 2212 13 -0.289723547262831 2212 14 -0.26327357678033536 2212 15 -0.17862855362843533 2212 16 -0.4144186052111323 2212 17 -0.48938903875421147 2212 18 -0.3779047049799402 2212 19 -0.4189645678599021 2212 20 -0.4978139996047304 2212 21 -0.05604821799770576 2212 22 -0.29020457103887765 2212 23 -0.1535343841794088 2212 24 -0.35664394897977303 2212 25 -0.0742668807463005 2213 1 -0.1514891961574243 2213 2 -0.1669321922108722 2213 3 -0.11360447586783451 2213 4 -0.007716852476136182 2213 5 -0.08701078605514567 2213 6 -0.23748307092458276 2213 7 -0.2624573183999743 2213 8 -0.20799979892471043 2213 9 -0.1683120748743363 2213 10 -0.10710600210367716 2213 11 0.0027671719323763 2213 12 0.21129401906137701 2213 13 0.22737691553898998 2213 14 -0.5375697515289986 2213 15 -0.17342778824666993 2213 16 -0.19893306845862924 2213 17 -0.12923971932887213 2213 18 -0.12042814433595339 2213 19 -0.1844476184108217 2213 20 -0.16257642002325667 2213 21 0.13838563706963924 2213 22 -0.15038243574163346 2213 23 -0.35748947048743673 2213 24 -0.5473706012448858 2213 25 -0.17583097778550894 2214 1 -0.131047731057663 2214 2 -0.025287382521636204 2214 3 -0.010988596133255868 2214 4 0.08549257161610768 2214 5 -0.02876263785306321 2214 6 0.08724104934444199 2214 7 -0.04815934042433412 2214 8 0.023802008853621714 2214 9 -0.05900642291076963 2214 10 0.04477064166417932 2214 11 0.09725880012123866 2214 12 -0.13531431558885743 2214 13 -0.17657229824804688 2214 14 -0.009358115221189678 2214 15 0.14128266320890503 2214 16 -0.055828699885715594 2214 17 -0.2818048376603812 2214 18 -0.18193807449610233 2214 19 -0.3654625839906913 2214 20 -0.20902099139368602 2214 21 -0.5309419806393763 2214 22 -0.6685542449779973 2214 23 -0.8288907638739644 2214 24 -0.35331749692485204 2214 25 -0.8524828178436721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 10.0 9 38.0 10 69.0 11 152.0 12 377.0 13 904.0 14 1847.0 15 3334.0 16 5360.0 17 7272.0 18 8652.0 19 9830.0 20 10543.0 21 11002.0 22 11725.0 23 12589.0 24 13738.0 25 15164.0 26 17462.0 27 19517.0 28 23245.0 29 29354.0 30 36974.0 31 49745.0 32 66739.0 33 91884.0 34 126433.0 35 177453.0 36 255906.0 37 408521.0 38 885829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12346593851882 20.94542674534351 12.793989040383416 24.13711827575425 2 15.419710288742397 22.021088157256187 38.53448212190187 24.02471943209955 3 18.017678201395064 26.560416473609084 30.671901300696923 24.750004024298935 4 12.15252928567673 18.147190751987612 37.48566621519861 32.214613747137044 5 12.44939535158606 38.86410924520201 34.51645509331767 14.170040309894263 6 29.893057818129197 39.042739068637054 17.01567393215335 14.048529181080388 7 25.571266786434744 34.923650023423434 21.826384959632595 17.678698230509223 8 24.768639230164855 36.38817331024941 20.476731816190735 18.366455643395007 9 25.20740384477525 16.273929917811813 21.70073107818718 36.81793515922576 10 15.401952697488921 30.055471683792646 32.79546839483695 21.747107223881482 11 32.03089812903551 25.608945989138356 23.226765973800585 19.13338990802555 12 21.382505057245936 27.797039338769437 30.926536116730276 19.89391948725435 13 27.597266332706255 23.35734488436657 27.065521990554686 21.979866792372498 14 21.03002539045868 23.60335098442336 28.867569901966068 26.499053723151896 15 23.036955163547606 29.99534247979931 25.668280288870527 21.299422067782558 16 20.69055107402498 30.52480612981276 27.0772643677262 21.707378428436062 17 19.653025997199425 29.461247936825032 28.803247727411957 22.08247833856359 18 19.88563911019042 27.938562629335944 31.1158333047308 21.05996495574283 19 20.60633603297792 27.931741468443878 31.861077717352813 19.600844781225383 20 21.533122935193084 26.771150313598657 31.67793965072287 20.01778710048539 21 21.776139544138896 26.959341624718792 31.112463754515208 20.152055076627104 22 21.445674355918527 26.86209789577822 31.12871810223361 20.563509646069644 23 21.331138982685598 27.000462273446907 31.228222315294822 20.44017642857267 24 21.075123247414595 26.882816875218047 31.166965217878662 20.875094659488695 25 21.141353367778397 26.969251436804083 31.337318324878478 20.552076870539043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1744.0 1 1744.0 2 2135.0 3 2526.0 4 2526.0 5 2526.0 6 6314.5 7 10103.0 8 10103.0 9 10103.0 10 12199.5 11 14296.0 12 14296.0 13 14296.0 14 17894.0 15 21492.0 16 21492.0 17 21492.0 18 35188.5 19 48885.0 20 48885.0 21 48885.0 22 71957.5 23 95030.0 24 95030.0 25 95030.0 26 125658.5 27 156287.0 28 156287.0 29 156287.0 30 182193.0 31 208099.0 32 208099.0 33 208099.0 34 223167.5 35 238236.0 36 238236.0 37 238236.0 38 235370.0 39 232504.0 40 232504.0 41 232504.0 42 237200.0 43 241896.0 44 241896.0 45 241896.0 46 249033.5 47 256171.0 48 256171.0 49 256171.0 50 244137.5 51 232104.0 52 232104.0 53 232104.0 54 211800.0 55 191496.0 56 191496.0 57 191496.0 58 166175.0 59 140854.0 60 140854.0 61 140854.0 62 121860.0 63 102866.0 64 102866.0 65 102866.0 66 79791.5 67 56717.0 68 56717.0 69 56717.0 70 43244.0 71 29771.0 72 29771.0 73 29771.0 74 21445.5 75 13120.0 76 13120.0 77 13120.0 78 9076.0 79 5032.0 80 5032.0 81 5032.0 82 3378.5 83 1725.0 84 1725.0 85 1725.0 86 1116.5 87 508.0 88 508.0 89 508.0 90 336.0 91 164.0 92 164.0 93 164.0 94 102.5 95 41.0 96 41.0 97 41.0 98 22.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09288903583526925 2 0.0 3 0.13616194495216738 4 0.012599541811145034 5 0.021679901254349557 6 0.014511196430767037 7 0.024199809616578565 8 0.021853688037951558 9 8.689339180100022E-5 10 0.397537267489576 11 0.06373630288603367 12 9.992740057115025E-4 13 4.344669590050011E-5 14 8.254872221095021E-4 15 7.385938303085019E-4 16 8.689339180100022E-5 17 0.0 18 4.3446695900500117E-4 19 4.3446695900500117E-4 20 1.3034008770150036E-4 21 2.1723347950250058E-4 22 0.002606801754030007 23 1.3034008770150036E-4 24 0.0 25 0.0015206343565175042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2301671.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.90205469693991 #Duplication Level Percentage of deduplicated Percentage of total 1 83.05670798459111 54.73607712548289 2 11.372432843495746 14.989333825786652 3 2.842792248687548 5.620375507951309 4 1.048747099669432 2.7645835490268804 5 0.48928183085796734 1.6122338989710334 6 0.28694395190661776 1.1346117608103612 7 0.1911827381611622 0.881953468718536 8 0.12510141571130515 0.6595552272696841 9 0.09582437336182592 0.5683520785131939 >10 0.43678380821434526 4.975995760753111 >50 0.026001102139411914 1.1850374460969064 >100 0.022643720086717844 2.9814596106718243 >500 0.003043055040209078 1.3733747495407047 >1k 0.0021169078540579545 2.2829270763661076 >5k 1.3230674087862215E-4 0.5412060866229135 >10k+ 2.646134817572443E-4 3.6929228274180055 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 31410 1.3646607182347088 No Hit GGTATCAACGCAGAGTACTTTTTTT 21847 0.949179965338226 No Hit TATCAACGCAGAGTACTTTTTTTTT 20473 0.8894842051709388 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10977 0.4769143808997897 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6692 0.29074528896614676 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5722 0.24860199394266166 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3966 0.17230959594138345 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3110 0.13511922425055536 No Hit GTACTGGTTCACTATCGGTCAGTCA 3088 0.13416339694074436 No Hit GGTATCAACGCAGAGTACATGGGGG 2479 0.10770435913733979 No Hit GTTAATGATAGTGTGTCGAAACACA 2424 0.10531479086281227 No Hit GTGGTATCAACGCAGAGTACTTTTT 2325 0.10101356796866276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.344669590050012E-5 0.0 6 0.0 0.0 0.0 4.344669590050012E-5 0.0 7 0.0 0.0 0.0 8.689339180100024E-5 0.0 8 0.0 0.0 0.0 8.689339180100024E-5 0.0 9 0.0 0.0 0.0 8.689339180100024E-5 0.0 10 0.0 0.0 0.0 8.689339180100024E-5 0.0 11 0.0 0.0 0.0 8.689339180100024E-5 0.0 12 0.0 0.0 0.0 8.689339180100024E-5 0.0 13 0.0 0.0 0.0 8.689339180100024E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 14.303131 1 CGCAGAA 120 1.2781129E-7 11.082102 2 TCTAGAC 175 2.0008883E-11 10.855937 3 CGATAGT 80 3.848034E-4 10.664488 18 TAGCACG 100 2.3315017E-5 10.477351 4 CTAGTAC 100 2.4026884E-5 10.448839 3 GTATCAA 9150 0.0 10.372339 1 TGTCCCG 495 0.0 10.157099 11 TACAGGG 835 0.0 10.124622 2 ACGGTAC 235 0.0 10.10526 3 ATACAGG 845 0.0 10.016144 1 GTGCTAG 190 9.822543E-11 10.010221 1 CTAGACC 105 3.990253E-5 9.97843 4 TAGACAG 210 5.456968E-12 9.97843 5 ACAGGGT 780 0.0 9.864288 3 CCGTACA 725 0.0 9.806426 17 CCCTATA 165 1.0244548E-8 9.786791 2 CCCCCGG 475 0.0 9.778899 18 GTCTTAG 205 4.0017767E-11 9.741654 1 CCCCGGT 475 0.0 9.5793295 19 >>END_MODULE