FastQCFastQC Report
Wed 25 May 2016
SRR1294667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576400
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC135620.3792081422659658No Hit
CCCATGTACTCTGCGTTGATACCAC128770.3600548037132312No Hit
GAGTACATGGGAAGCAGTGGTATCA63750.178251873392238No Hit
GTATCAACGCAGAGTACTTTTTTTT54290.15180069343473884No Hit
CATGTACTCTGCGTTGATACCACTG48940.13684151660888044No Hit
ATTCCATTCCATTCCATTCCATTCC47470.13273123811654178No Hit
GCGTTGATACCACTGCTTCCCATGT41600.11631808522536628No Hit
GTATCAACGCAGAGTACATGGGAAG38540.10776199530253885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT500.001503421513.2962296
CGCCCTA1850.011.81589416
TCGCGCG1354.7584763E-911.255919
CCCCGTA1701.0913936E-1111.18096715
GCGCCTT1553.110472E-1011.03685213
CGCGCGC1605.475158E-1010.684319510
TGGACCG1002.4079194E-510.4471845
GCGCGCC1553.6889105E-910.41567111
GCCCCGT1659.240466E-1010.36780614
TATCGCG1502.4590918E-810.130467
CGCCTTT1606.1409082E-910.09781114
CCCGTAC1803.9108272E-1010.03209416
CTAGGAC3150.09.9501163
GTCTAGA3700.09.7575411
TAGACAG5950.09.7368715
CCCGCCC2251.8189894E-129.71501814
CGGTCAG3800.09.50408816
GTATACG900.00111344089.5007631
TAGGACT4100.09.4975724
TTAGGAC2850.09.3312093
Produced by FastQC (version 0.11.5)