##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3576400 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.107727323565598 31.0 30.0 34.0 25.0 34.0 2 30.19588748462141 31.0 30.0 34.0 25.0 34.0 3 30.210719158930768 31.0 30.0 34.0 25.0 34.0 4 33.84795828207135 35.0 35.0 37.0 28.0 37.0 5 34.16747791074824 37.0 35.0 37.0 30.0 37.0 6 34.18991695559781 37.0 35.0 37.0 30.0 37.0 7 34.12715244379823 37.0 35.0 37.0 29.0 37.0 8 33.9882801140812 37.0 35.0 37.0 28.0 37.0 9 35.185427245274575 39.0 35.0 39.0 27.0 39.0 10 35.26286209596242 39.0 35.0 39.0 28.0 39.0 11 35.500721395817024 39.0 35.0 39.0 28.0 39.0 12 35.27244100212504 39.0 35.0 39.0 27.0 39.0 13 35.147093725534056 38.0 35.0 39.0 27.0 39.0 14 36.06050134213175 39.0 35.0 41.0 27.0 41.0 15 36.03982384520747 39.0 35.0 41.0 27.0 41.0 16 36.13871071468516 39.0 35.0 41.0 27.0 41.0 17 36.105033273683034 39.0 35.0 41.0 27.0 41.0 18 35.9855989262946 39.0 35.0 41.0 27.0 41.0 19 36.25254222122805 39.0 35.0 41.0 27.0 41.0 20 36.366488368191476 39.0 36.0 41.0 27.0 41.0 21 36.387313219997765 39.0 36.0 41.0 27.0 41.0 22 36.3746499273012 39.0 36.0 41.0 27.0 41.0 23 36.3380144838385 39.0 36.0 41.0 27.0 41.0 24 36.402892573537635 39.0 36.0 41.0 27.0 41.0 25 35.101932669723745 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9444899809192648 1101 2 0.8955381583528776 1101 3 1.178544639654799 1101 4 0.9479673661909942 1101 5 0.6755452758793723 1101 6 0.688349216043882 1101 7 0.7822081161697128 1101 8 0.877548521240314 1101 9 1.1546357467807198 1101 10 1.0716085391645436 1101 11 0.8554815435405487 1101 12 1.1020052989397868 1101 13 1.111058744987254 1101 14 1.190581212852642 1101 15 1.3461974099878375 1101 16 1.254080154176691 1101 17 1.2794632863986806 1101 18 1.3750948698137506 1101 19 1.226277809909071 1101 20 1.0885414758746208 1101 21 0.9320171306655567 1101 22 0.8621878881704959 1101 23 0.970161607952015 1101 24 1.0024785922054065 1101 25 0.9886494144214737 1102 1 0.8901151087681036 1102 2 0.8152658564471693 1102 3 1.0779573182054953 1102 4 0.9727647891269413 1102 5 0.7604182931857224 1102 6 0.5737138486162934 1102 7 0.6897005343111289 1102 8 0.7232183693082845 1102 9 1.0244177787126816 1102 10 0.9745867337576968 1102 11 0.6808725536707811 1102 12 0.8675787773926871 1102 13 0.9470796529182692 1102 14 1.0632390374474099 1102 15 1.2560646069085308 1102 16 1.2003196936075753 1102 17 1.1108270257952881 1102 18 1.2482224409959883 1102 19 0.8586239812624541 1102 20 0.3076934625764949 1102 21 0.5265306723533385 1102 22 0.5836505219088295 1102 23 0.7386306591646559 1102 24 0.7950100084664911 1102 25 0.790388527228842 1103 1 0.714633436565645 1103 2 0.7129247456628427 1103 3 0.9898906083460695 1103 4 0.7941535291602904 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0.3065284609035501 2207 9 -1.3072954766030733 2207 10 -0.32484524349651167 2207 11 -0.005682878541115599 2207 12 0.32504088624934724 2207 13 0.4900320735697363 2207 14 0.6567501930372899 2207 15 0.5482611600210063 2207 16 0.5762869365330658 2207 17 0.6781004069986025 2207 18 0.7227328165285556 2207 19 -0.5354141055618058 2207 20 0.06691243352299381 2207 21 -0.362504174848155 2207 22 0.12809894613676676 2207 23 -0.6054309064071148 2207 24 -1.7162170397616734 2207 25 -0.37908021315190865 2208 1 0.5115959972953661 2208 2 0.2591082192971008 2208 3 0.6628057010583639 2208 4 0.6041430483358496 2208 5 0.4298437904664567 2208 6 0.40301152144442653 2208 7 0.4512153448061582 2208 8 0.650165065379845 2208 9 -0.029056783957841503 2208 10 0.17904966276400103 2208 11 0.3572455665356671 2208 12 0.30384533166905214 2208 13 0.6345392077765908 2208 14 0.9758812387362852 2208 15 0.8617406096773124 2208 16 0.6192534589411167 2208 17 0.5766638383986162 2208 18 0.83875668028098 2208 19 -1.130917379489425 2208 20 0.08513462230389024 2208 21 -0.11434983539324861 2208 22 0.1945632101589183 2208 23 0.4584364737040758 2208 24 0.5540114518818271 2208 25 0.5710856885445281 2209 1 0.15133041655715118 2209 2 -1.2849681030445268 2209 3 -0.22752226451212465 2209 4 0.10795864581929493 2209 5 0.10111252138598559 2209 6 0.22172470378886544 2209 7 0.111028072218879 2209 8 0.32791790769931595 2209 9 -1.991488238345866 2209 10 -0.42283770632680984 2209 11 -0.14005143713128376 2209 12 0.24667660259173374 2209 13 0.3200419968372614 2209 14 0.582307249626119 2209 15 0.6867324260079002 2209 16 0.5714329602309007 2209 17 0.34732138029357174 2209 18 0.6663915502232314 2209 19 0.38767228022580724 2209 20 0.3826399375558438 2209 21 0.4012740530802219 2209 22 0.2945024171861519 2209 23 0.2987025834875894 2209 24 0.4417848844552381 2209 25 0.40798914864246427 2210 1 0.14514510213519927 2210 2 0.183450026484401 2210 3 0.1493036083887631 2210 4 0.17150996307245947 2210 5 0.10632918215230092 2210 6 0.16444539263646618 2210 7 0.14173620197080794 2210 8 0.18877022146653388 2210 9 0.3578291532127267 2210 10 -1.4111387896473886 2210 11 -0.4283412557018096 2210 12 -2.2533170711552657 2210 13 -0.6223186028379288 2210 14 -0.4094866718688124 2210 15 -0.6682471438317847 2210 16 -0.05188474697039425 2210 17 0.06457749390886391 2210 18 0.40090470581469617 2210 19 0.2142784522177692 2210 20 0.3115798652374906 2210 21 0.2638361587481697 2210 22 0.1913611789460674 2210 23 0.13664624308425743 2210 24 0.14304882386562667 2210 25 0.13645727758279946 2211 1 -0.29905883917449927 2211 2 -0.08115836609645655 2211 3 -1.5094186143196922 2211 4 -0.5485394041921765 2211 5 -0.37775348528455766 2211 6 -0.41786198848193123 2211 7 -0.4060317806376048 2211 8 -0.03006423117580681 2211 9 0.2606783465030986 2211 10 0.15522506309005024 2211 11 -0.14250829433693468 2211 12 -0.16708469346481536 2211 13 -0.16464612130749856 2211 14 -0.725995422122871 2211 15 -0.2184306118031074 2211 16 -0.05791075753332109 2211 17 -0.1315297467853611 2211 18 0.04867105954162554 2211 19 -0.049811737247893006 2211 20 0.018455896659830273 2211 21 -0.10788000403494635 2211 22 -0.16429779842754755 2211 23 -0.4675577419864325 2211 24 -0.3180620562846812 2211 25 -0.18112416405997322 2212 1 -0.4218273531135601 2212 2 -0.3622700476110019 2212 3 -2.5091627153395315 2212 4 -1.5079918064601614 2212 5 -0.7380362940902003 2212 6 -0.4059664683053015 2212 7 -0.3423947126921192 2212 8 -0.3274552354267044 2212 9 -1.0385436006038091 2212 10 -0.28907882386155137 2212 11 -0.3456190451200172 2212 12 -0.05412890112226876 2212 13 -0.4339281485908728 2212 14 -0.17725710061149869 2212 15 0.11030606110321628 2212 16 -1.126945311460446 2212 17 -1.395022483532351 2212 18 -0.3545486504708393 2212 19 -0.19324272434494816 2212 20 -0.24071381679591752 2212 21 -0.1527010625646028 2212 22 -0.4817497436771703 2212 23 -0.11351289471195258 2212 24 -0.19854797218912523 2212 25 0.05824937645501649 2213 1 -3.023211100911027 2213 2 -1.348671596954258 2213 3 -2.3694170947218787 2213 4 -1.0110477030533218 2213 5 -0.7961280477528874 2213 6 -0.6398790679756416 2213 7 -0.3418832134344143 2213 8 -0.16044628145788664 2213 9 -0.363918919899362 2213 10 -0.165040242438387 2213 11 -0.4235721949427358 2213 12 -0.21393281368124661 2213 13 -0.6468401089500091 2213 14 -0.20426885633892766 2213 15 -0.360285714848267 2213 16 -1.5353602206385233 2213 17 -1.1007921311503992 2213 18 -0.3932516218574662 2213 19 -0.2553402430100391 2213 20 -0.07689161178902992 2213 21 0.03576294779897893 2213 22 0.0035276731745383927 2213 23 0.04012968638622283 2213 24 0.06814415170986621 2213 25 -0.004273915335204492 2214 1 -0.7045398096389128 2214 2 -0.5537801152082729 2214 3 -6.542921259577305 2214 4 -2.040694875652285 2214 5 -1.1271288339976593 2214 6 -0.6138045668748404 2214 7 -0.47720853434746857 2214 8 -0.2984744908940158 2214 9 -0.10438663818109006 2214 10 -0.022181553472194082 2214 11 -0.6826673626416948 2214 12 -0.23070181585676863 2214 13 -4.991551693660092 2214 14 -3.5975977436424103 2214 15 -1.9167101192713574 2214 16 -2.5777681076886836 2214 17 -3.153130386763486 2214 18 -0.9633121283521646 2214 19 -0.42652360154253444 2214 20 -0.0700319124405624 2214 21 -0.22344697018138504 2214 22 -0.1527403713072104 2214 23 -0.09810806914271808 2214 24 0.041945623673328214 2214 25 0.19972594609477312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1370.0 3 175.0 4 181.0 5 387.0 6 51.0 7 63.0 8 241.0 9 610.0 10 1548.0 11 2812.0 12 5074.0 13 8158.0 14 11777.0 15 15720.0 16 18598.0 17 21206.0 18 22262.0 19 23588.0 20 24419.0 21 25259.0 22 26527.0 23 27812.0 24 30349.0 25 33022.0 26 36931.0 27 41503.0 28 48289.0 29 59280.0 30 71798.0 31 90195.0 32 116995.0 33 155120.0 34 206582.0 35 282824.0 36 397154.0 37 598507.0 38 1170013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70154895777997 21.00183774651861 12.996892680193481 23.299720615507937 2 14.580067164187454 21.082219618612154 41.30466042471669 23.033052792483698 3 18.537101227894954 26.864041897823697 29.386416219739765 25.21244065454159 4 11.818451398485976 17.322986196979784 38.63002086958468 32.228541534949564 5 12.799385681855375 38.55973726429807 35.12934669950863 13.511530354337923 6 30.831843174166334 38.56432129463797 17.65818327516793 12.945652256027765 7 25.92937044799647 32.939165605564234 23.63820058291386 17.49326336352544 8 24.04519807521803 37.9820173503444 21.257655160296377 16.7151294141412 9 24.30396286570856 17.274104623459905 22.212305070850896 36.20962743998064 10 14.656053523458901 30.425658893711606 33.643960550175585 21.274327032653908 11 32.46805423554687 26.404395202252996 22.619584859639517 18.507965702560615 12 20.626692850446098 27.846863500122964 32.20602072114694 19.320422928283993 13 26.425905512251624 24.098768402616518 26.98488061730951 22.49044546782235 14 19.85944027358757 23.13995062866889 28.703612254587746 28.296996843155792 15 22.98018714934679 30.458354737658517 24.218084612276737 22.343373500717952 16 21.3636680298572 31.693494483960528 26.128129465143797 20.814708021038477 17 19.873613503311027 30.591984143771494 29.024435692630007 20.509966660287468 18 20.200269146141427 30.236911326818795 29.73006422882014 19.832755298219638 19 20.61697191809933 29.064849735823696 29.942564772370204 20.37561357370677 20 22.028970260884552 28.668146926204514 28.87311885238104 20.429763960529897 21 22.090637294702425 29.36109934469289 28.236092979635735 20.312170380968954 22 20.635126261135444 29.010472960590207 29.530049990752815 20.824350787521535 23 20.43601357894363 29.54116925191302 29.227744446586417 20.795072722556935 24 20.607122171515233 28.71404148397693 29.196174497958815 21.482661846549018 25 21.4075942736844 28.527183882510027 28.12250626476655 21.942715579039028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3367.0 1 3367.0 2 3202.5 3 3038.0 4 3038.0 5 3038.0 6 4716.5 7 6395.0 8 6395.0 9 6395.0 10 10220.5 11 14046.0 12 14046.0 13 14046.0 14 23690.0 15 33334.0 16 33334.0 17 33334.0 18 52687.5 19 72041.0 20 72041.0 21 72041.0 22 103037.0 23 134033.0 24 134033.0 25 134033.0 26 174422.5 27 214812.0 28 214812.0 29 214812.0 30 259254.0 31 303696.0 32 303696.0 33 303696.0 34 342303.5 35 380911.0 36 380911.0 37 380911.0 38 408454.5 39 435998.0 40 435998.0 41 435998.0 42 434001.0 43 432004.0 44 432004.0 45 432004.0 46 439812.0 47 447620.0 48 447620.0 49 447620.0 50 411509.5 51 375399.0 52 375399.0 53 375399.0 54 328059.0 55 280719.0 56 280719.0 57 280719.0 58 238755.0 59 196791.0 60 196791.0 61 196791.0 62 162049.0 63 127307.0 64 127307.0 65 127307.0 66 96905.0 67 66503.0 68 66503.0 69 66503.0 70 48346.5 71 30190.0 72 30190.0 73 30190.0 74 21029.5 75 11869.0 76 11869.0 77 11869.0 78 8014.0 79 4159.0 80 4159.0 81 4159.0 82 2824.5 83 1490.0 84 1490.0 85 1490.0 86 973.5 87 457.0 88 457.0 89 457.0 90 304.5 91 152.0 92 152.0 93 152.0 94 97.5 95 43.0 96 43.0 97 43.0 98 34.5 99 26.0 100 26.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09914998322335308 2 0.05245498266413153 3 0.05564254557655743 4 0.05088916228609776 5 0.047673638295492676 6 0.06702270439548148 7 0.06394698579577228 8 0.060871267196063075 9 0.06556872833016442 10 0.0654568840174477 11 0.05980874622525445 12 0.060871267196063075 13 0.06095515043060061 14 0.06321999776311374 15 0.06587630019013534 16 0.061150877977854824 17 0.06386310256123476 18 0.14276926518286545 19 0.06881221339894866 20 0.06296834805950118 21 0.061458449837825745 22 0.06540096186108936 23 0.06534503970473102 24 0.06962308466614472 25 0.0683368750699027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3576400.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.8797915143462 #Duplication Level Percentage of deduplicated Percentage of total 1 90.28472423611747 72.11924948911303 2 7.306159712150648 11.672290291542188 3 1.288654729514951 3.0881241338289147 4 0.39496733307335974 1.2619963288350924 5 0.18600812315926973 0.7429145048968647 6 0.11543535995159157 0.5532571491789986 7 0.0820154168880606 0.45859620813863283 8 0.05130489509966323 0.32785794593812007 9 0.04058507385779744 0.2917734514519681 >10 0.21996229120257202 3.2165979205612634 >50 0.015739156069018084 0.8808533694920188 >100 0.0122240089297333 1.8416579407556306 >500 0.0010217501090676537 0.5944545440551186 >1k 0.0010569828324049672 1.87407123354884 >5k 7.046552216033108E-5 0.33221007735227287 >10k+ 7.046552216033108E-5 0.7440954113111439 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 13562 0.3792081422659658 No Hit CCCATGTACTCTGCGTTGATACCAC 12877 0.3600548037132312 No Hit GAGTACATGGGAAGCAGTGGTATCA 6375 0.178251873392238 No Hit GTATCAACGCAGAGTACTTTTTTTT 5429 0.15180069343473884 No Hit CATGTACTCTGCGTTGATACCACTG 4894 0.13684151660888044 No Hit ATTCCATTCCATTCCATTCCATTCC 4747 0.13273123811654178 No Hit GCGTTGATACCACTGCTTCCCATGT 4160 0.11631808522536628 No Hit GTATCAACGCAGAGTACATGGGAAG 3854 0.10776199530253885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.796107817917459E-5 0.0 0.0 0.0 0.0 6 2.796107817917459E-5 0.0 0.0 0.0 0.0 7 2.796107817917459E-5 0.0 0.0 5.592215635834918E-5 0.0 8 2.796107817917459E-5 0.0 0.0 8.388323453752377E-5 0.0 9 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 10 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 11 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 12 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 13 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 50 0.0015034215 13.296229 6 CGCCCTA 185 0.0 11.815894 16 TCGCGCG 135 4.7584763E-9 11.25591 9 CCCCGTA 170 1.0913936E-11 11.180967 15 GCGCCTT 155 3.110472E-10 11.036852 13 CGCGCGC 160 5.475158E-10 10.6843195 10 TGGACCG 100 2.4079194E-5 10.447184 5 GCGCGCC 155 3.6889105E-9 10.415671 11 GCCCCGT 165 9.240466E-10 10.367806 14 TATCGCG 150 2.4590918E-8 10.13046 7 CGCCTTT 160 6.1409082E-9 10.097811 14 CCCGTAC 180 3.9108272E-10 10.032094 16 CTAGGAC 315 0.0 9.950116 3 GTCTAGA 370 0.0 9.757541 1 TAGACAG 595 0.0 9.736871 5 CCCGCCC 225 1.8189894E-12 9.715018 14 CGGTCAG 380 0.0 9.504088 16 GTATACG 90 0.0011134408 9.500763 1 TAGGACT 410 0.0 9.497572 4 TTAGGAC 285 0.0 9.331209 3 >>END_MODULE