FastQCFastQC Report
Wed 25 May 2016
SRR1294667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576400
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC144690.40456884017447714No Hit
GTACATGGGAAGCAGTGGTATCAAC136570.38186444469298736No Hit
GTATCAACGCAGAGTACTTTTTTTT132150.3695056481377922No Hit
GGTATCAACGCAGAGTACTTTTTTT90680.2535510569287552No Hit
TATCAACGCAGAGTACTTTTTTTTT79110.22120008947545017No Hit
GAGTACATGGGAAGCAGTGGTATCA67290.1881500950676658No Hit
CATGTACTCTGCGTTGATACCACTG54910.15353428028184768No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51080.1428251873392238No Hit
ATTCCATTCCATTCCATTCCATTCC46750.13071804048764118No Hit
GCGTTGATACCACTGCTTCCCATGT45490.1271949446370652No Hit
GAATGGAATGGAATGGAATGGAATG40630.11360586064198634No Hit
ACGCAGAGTACATGGGAAGCAGTGG40000.11184431271669835No Hit
GTATCAACGCAGAGTACATGGGAAG39390.11013868694776871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG752.0367553E-411.4230065
CGCCCTA2200.011.20730516
AATACCG600.00579063911.1056995
TCGTAGG600.005869744311.0854742
TGTCCCG2600.010.58411511
TGGACCG909.329445E-510.5768575
GTGCGCC909.6324635E-510.54356311
CGTGAAA1105.910293E-610.38411310
GTCCCGC2600.010.21271612
TCGCGCG1701.5097612E-910.0786979
CAATCCG951.6127338E-410.01975810
TATTCCG1553.943933E-89.82624155
CGCGCGC2054.0017767E-119.75093610
CGCGCCT2253.6379788E-129.69397512
CTAGAGC3050.09.6576033
TAGACAG7100.09.6532435
ACTGCGT900.00109390089.5191718
CGAACCG900.00113240239.48323619
GCGCGCC2650.09.31016411
TATCGCG1856.4792403E-99.2618967
Produced by FastQC (version 0.11.5)