##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294667_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3576400 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43731601610558 31.0 31.0 34.0 28.0 34.0 2 31.596101386869478 31.0 31.0 34.0 28.0 34.0 3 31.680170562576894 31.0 31.0 34.0 29.0 34.0 4 35.28718152331954 37.0 35.0 37.0 32.0 37.0 5 35.443969075047534 37.0 35.0 37.0 33.0 37.0 6 35.08969019125377 37.0 35.0 37.0 32.0 37.0 7 34.94375237669165 37.0 35.0 37.0 32.0 37.0 8 34.996665361816355 37.0 35.0 37.0 32.0 37.0 9 36.461351918129964 39.0 37.0 39.0 32.0 39.0 10 36.223242366625655 39.0 37.0 39.0 32.0 39.0 11 36.409470417179286 39.0 37.0 39.0 32.0 39.0 12 36.33289760653171 39.0 37.0 39.0 32.0 39.0 13 36.212683704283634 39.0 35.0 39.0 31.0 39.0 14 37.339888435298064 40.0 37.0 41.0 31.0 41.0 15 37.410272340901464 40.0 37.0 41.0 31.0 41.0 16 37.446072866569736 40.0 37.0 41.0 32.0 41.0 17 37.47797002572419 40.0 37.0 41.0 32.0 41.0 18 37.474295380829886 40.0 37.0 41.0 32.0 41.0 19 37.476965943406775 40.0 37.0 41.0 31.0 41.0 20 37.53865507213958 40.0 37.0 41.0 31.0 41.0 21 37.387192148529245 40.0 37.0 41.0 31.0 41.0 22 37.379564366401965 40.0 37.0 41.0 31.0 41.0 23 37.39109215971368 40.0 37.0 41.0 31.0 41.0 24 37.329331730231516 40.0 37.0 41.0 31.0 41.0 25 36.317774298176936 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5434859827649099 1101 2 0.5044352165009656 1101 3 0.4521697174879691 1101 4 0.3493722791767553 1101 5 0.3690406724774462 1101 6 0.44989692915250856 1101 7 0.5680790857352136 1101 8 0.5887468565901344 1101 9 0.7442447901613747 1101 10 0.8043963362504059 1101 11 0.7723269660919456 1101 12 0.7611745458566403 1101 13 0.7393811167989242 1101 14 0.938191342424318 1101 15 0.9109273531386037 1101 16 0.9759616543218286 1101 17 1.0370364887383587 1101 18 0.9399231038421476 1101 19 1.0852050122950416 1101 20 1.0214592730515974 1101 21 1.290760467915952 1101 22 1.2429629487257117 1101 23 1.115946489095542 1101 24 1.3268986902528823 1101 25 1.175858403136111 1102 1 0.38995790762019666 1102 2 0.39994593307490334 1102 3 0.35233686544984977 1102 4 0.27688098825514373 1102 5 0.3233299742093081 1102 6 0.38218756880521454 1102 7 0.46206581341303377 1102 8 0.47964543199315557 1102 9 0.5972514570368972 1102 10 0.6968873937423083 1102 11 0.6602680484318171 1102 12 0.6780059954736046 1102 13 0.7335013908454115 1102 14 0.9196352772138852 1102 15 0.8848372824401167 1102 16 0.8599617673509528 1102 17 0.8068559308536223 1102 18 0.7671586387248439 1102 19 0.9462779658382274 1102 20 0.8761661068293662 1102 21 1.1630794508503968 1102 22 1.1210813700774054 1102 23 0.9801609151747996 1102 24 1.0992366547089105 1102 25 0.9999140724374627 1103 1 0.3435900830223808 1103 2 0.30540622618786273 1103 3 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0.19271729000915272 2207 2 0.20296561107980438 2207 3 0.18503533937570538 2207 4 0.2066426486010613 2207 5 0.15384861307595088 2207 6 0.27044265495369757 2207 7 0.23494168297844453 2207 8 0.2162263871589687 2207 9 0.3078965878409221 2207 10 0.48920828793151827 2207 11 0.35252646100777696 2207 12 0.33266843033674576 2207 13 0.420906442642341 2207 14 0.4410305043770606 2207 15 0.4103683835593017 2207 16 0.4540594620490239 2207 17 0.5787845329452921 2207 18 0.5285607036103244 2207 19 0.6662988345192957 2207 20 0.4753595044574368 2207 21 0.8396986178629504 2207 22 0.6858081441152706 2207 23 0.642082475123523 2207 24 0.7642977601861105 2207 25 0.7265255218301689 2208 1 0.1760436569303181 2208 2 0.17383372521528884 2208 3 0.2011897673293177 2208 4 0.12478227991430657 2208 5 0.09459230165434462 2208 6 0.23901579164824938 2208 7 0.20624920081956333 2208 8 0.1927641213193425 2208 9 0.37091817589870857 2208 10 0.4044205193682231 2208 11 0.06335400115667511 2208 12 -0.12664639703975666 2208 13 0.06143511178868977 2208 14 -0.30552180822859754 2208 15 0.2563514148860335 2208 16 0.2895780744808576 2208 17 0.20325690470775726 2208 18 0.3672151265572765 2208 19 0.5598942651632512 2208 20 0.49529616494159256 2208 21 0.7801016054953465 2208 22 0.7592442179617152 2208 23 0.6908673106413374 2208 24 0.7373050635227685 2208 25 0.7186373498634495 2209 1 -0.032684362300475556 2209 2 -0.014921133617004756 2209 3 0.0912084303468319 2209 4 -0.5246655999130496 2209 5 -0.08003487493010653 2209 6 0.06354119786765011 2209 7 0.04487460888118022 2209 8 0.0795331236107728 2209 9 0.15423274859307412 2209 10 0.17697941226536074 2209 11 0.2331961519903203 2209 12 0.18131483716623364 2209 13 0.26484858761155294 2209 14 0.30421782118462204 2209 15 -0.3085719373150084 2209 16 -0.3008125735098801 2209 17 0.034836468632725826 2209 18 -0.29022077039414995 2209 19 0.1355056549416247 2209 20 0.1327020291252765 2209 21 0.442083702035724 2209 22 0.47498846423986407 2209 23 0.4004742536050543 2209 24 0.4744028060581513 2209 25 0.42034451290056296 2210 1 -0.0858841112027875 2210 2 -0.10205683691210155 2210 3 -0.06917148172303911 2210 4 -0.05120472893636219 2210 5 -0.04439146389488258 2210 6 -0.35171814548643 2210 7 -0.10312586927253165 2210 8 -0.27227954885977823 2210 9 -0.0550076004974116 2210 10 0.13890941902325693 2210 11 0.11144816904575094 2210 12 0.11903435982029009 2210 13 0.18110620603283678 2210 14 0.08300058311183278 2210 15 -0.0592778887686265 2210 16 0.16593965548937462 2210 17 -0.8945876231743171 2210 18 -1.4664766297151246 2210 19 -0.29041324287771175 2210 20 -0.18748203899612292 2210 21 -0.28571543282764367 2210 22 -0.25279233310345717 2210 23 -0.22281870368661316 2210 24 0.17906156021890496 2210 25 0.186325006028639 2211 1 -0.28243692391642483 2211 2 -0.21451858250901523 2211 3 -0.1424077556007397 2211 4 -0.07694087396224347 2211 5 -0.11395643341291617 2211 6 -0.0851352203459328 2211 7 -0.16306664119703385 2211 8 -0.09612258108829508 2211 9 -0.12891037863986554 2211 10 0.08318356026235563 2211 11 -0.25476766212627666 2211 12 -0.16623372003476788 2211 13 -0.12402280138027066 2211 14 -0.13318578232359357 2211 15 -0.23547399831892335 2211 16 -0.20200971887426533 2211 17 -0.3157469637641981 2211 18 -0.36456243870519245 2211 19 -0.3229593888468898 2211 20 -0.34331640071283687 2211 21 -0.030466058366755533 2211 22 -0.06046513068551462 2211 23 -0.7916286137042832 2211 24 -0.1598077213130793 2211 25 -0.822322956702557 2212 1 -0.2413129665547764 2212 2 -0.3437152214415562 2212 3 -0.18046796829165856 2212 4 -0.03334400902473078 2212 5 -0.0753236434931992 2212 6 -0.09371148815452557 2212 7 -0.2127024351255713 2212 8 -0.2904597270816609 2212 9 -0.09685296649068675 2212 10 -0.5213753022418999 2212 11 -0.21170881109965478 2212 12 -0.28998370177490074 2212 13 -0.2820611596200635 2212 14 -0.29806767750987717 2212 15 -0.11651514457940948 2212 16 -0.4518993771121771 2212 17 -0.5757338062264239 2212 18 -0.3933821157455526 2212 19 -0.5089645579541155 2212 20 -0.6593341639105503 2212 21 -0.08880424360735617 2212 22 -0.43971746220955765 2212 23 -0.26161956724492086 2212 24 -0.44724579428159217 2212 25 -0.18947082301112061 2213 1 -0.16495578585730186 2213 2 -0.16215883995852565 2213 3 -0.11308114724662843 2213 4 -0.036524951469942835 2213 5 -0.09161238593289767 2213 6 -0.29091967834236243 2213 7 -0.3368709087848103 2213 8 -0.3273859043540668 2213 9 -0.38178964596872333 2213 10 -0.31548609524428883 2213 11 -0.1435661370286283 2213 12 0.018676736190990084 2213 13 0.046861552762088365 2213 14 -0.6986819658295786 2213 15 -0.22483029597152893 2213 16 -0.37451375714741886 2213 17 -0.2833483259381069 2213 18 -0.30597330491880115 2213 19 -0.34233421630736416 2213 20 -0.5723058628109285 2213 21 -0.09588010145047576 2213 22 -0.42054345247098013 2213 23 -0.5111766146028245 2213 24 -0.7070380846630258 2213 25 -0.5664084222048302 2214 1 -0.1587856028313297 2214 2 -0.04629966015102127 2214 3 -0.019265858866127417 2214 4 0.03347644051136456 2214 5 -0.035141622396118066 2214 6 -0.05280868926112703 2214 7 0.02101280033274122 2214 8 -0.05050043281713812 2214 9 -0.08224415854649436 2214 10 -0.03726304611140563 2214 11 0.061270604243112814 2214 12 -0.06114592703306698 2214 13 -0.09115583391065485 2214 14 0.029168971090761886 2214 15 0.13793790986119348 2214 16 -0.20738927309488275 2214 17 -0.1941155598480222 2214 18 -0.14168032633632777 2214 19 -0.43045426703713474 2214 20 -0.3127718900358758 2214 21 -0.4233055588221504 2214 22 -0.562825491075678 2214 23 -0.9086702365406083 2214 24 -0.4087983829693016 2214 25 -0.604114978146896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 23.0 9 60.0 10 106.0 11 184.0 12 478.0 13 1172.0 14 2335.0 15 4046.0 16 6063.0 17 8024.0 18 9784.0 19 10916.0 20 12350.0 21 13468.0 22 14981.0 23 17026.0 24 19310.0 25 22126.0 26 25711.0 27 29199.0 28 34717.0 29 44052.0 30 56331.0 31 74262.0 32 100479.0 33 138425.0 34 190663.0 35 270501.0 36 391903.0 37 633144.0 38 1444555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.470995953782946 21.323818551920574 12.24035934280949 24.964826151486985 2 14.361704507325804 21.568308913991725 40.05175595570965 24.018230622972823 3 18.289028889674068 26.547361135764614 29.61596158271939 25.547648391841925 4 11.867664400893856 17.469306920725543 38.64496588197493 32.01806279640567 5 13.035457736010844 38.24296784128242 35.06837576479979 13.653198657906948 6 31.139186913478195 38.80343539094742 17.204611692432284 12.852766003142099 7 26.00591332052077 33.19911087643778 23.29538971310439 17.499586089937065 8 24.016288751996452 38.27308577659486 20.850883416733808 16.85974205467488 9 24.14306141884815 16.777933303769103 22.348907866415164 36.73009741096758 10 14.4749200154271 30.36101547145004 33.64154785236018 21.522516660762676 11 32.73718807646067 25.95909484781089 22.514369460276832 18.789347615451614 12 20.671977684442105 27.713703874269193 32.22415436567954 19.390164075609164 13 26.6483614808187 23.65182865451292 27.086315848339108 22.61349401632927 14 19.855909199196052 22.751454273567845 28.95950514993474 28.43313137730136 15 23.113933224805795 30.09941957712287 24.312526877902044 22.474120320169288 16 21.330244189961068 31.409049780840824 26.27343280777632 20.98727322142179 17 19.66791177382613 30.487957300066356 29.214022260939004 20.63010866516851 18 19.92911846862339 30.05172813926893 30.07949356753598 19.939659824571702 19 20.458565528734443 28.791540025221103 30.423502042573897 20.326392403470557 20 21.854856636361475 28.26273384231887 29.47610652626458 20.406302995055075 21 21.942660680003378 29.130375456395463 28.61692531639411 20.310038547207046 22 20.362431762327798 28.78723100243353 29.958613500538405 20.891723734700264 23 20.167101042137638 29.299611145177035 29.725178874057395 20.808108938627935 24 20.351079297617716 28.470305334973716 29.82269880326585 21.355916564142714 25 21.22934551561344 28.248109381037946 28.612280369159325 21.91026473418929 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1698.0 1 1698.0 2 1844.5 3 1991.0 4 1991.0 5 1991.0 6 3940.5 7 5890.0 8 5890.0 9 5890.0 10 9412.0 11 12934.0 12 12934.0 13 12934.0 14 21411.5 15 29889.0 16 29889.0 17 29889.0 18 48834.5 19 67780.0 20 67780.0 21 67780.0 22 99614.5 23 131449.0 24 131449.0 25 131449.0 26 173314.5 27 215180.0 28 215180.0 29 215180.0 30 261007.5 31 306835.0 32 306835.0 33 306835.0 34 344746.5 35 382658.0 36 382658.0 37 382658.0 38 409646.0 39 436634.0 40 436634.0 41 436634.0 42 434119.5 43 431605.0 44 431605.0 45 431605.0 46 441627.5 47 451650.0 48 451650.0 49 451650.0 50 416029.5 51 380409.0 52 380409.0 53 380409.0 54 331976.5 55 283544.0 56 283544.0 57 283544.0 58 240741.0 59 197938.0 60 197938.0 61 197938.0 62 162561.5 63 127185.0 64 127185.0 65 127185.0 66 96316.5 67 65448.0 68 65448.0 69 65448.0 70 47372.5 71 29297.0 72 29297.0 73 29297.0 74 20227.5 75 11158.0 76 11158.0 77 11158.0 78 7409.5 79 3661.0 80 3661.0 81 3661.0 82 2401.5 83 1142.0 84 1142.0 85 1142.0 86 727.5 87 313.0 88 313.0 89 313.0 90 200.0 91 87.0 92 87.0 93 87.0 94 51.5 95 16.0 96 16.0 97 16.0 98 12.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09599038138910636 2 0.0 3 0.13069007940946203 4 0.012051224695224249 5 0.0221172128397271 6 0.012470640867911865 7 0.020803042165305895 8 0.019041494240017894 9 1.6776646907504754E-4 10 0.38692539984341795 11 0.06506542892293926 12 0.00170562576892965 13 0.0 14 0.001230287439883682 15 0.0011743652835253327 16 1.1184431271669834E-4 17 2.7961078179174586E-5 18 2.796107817917459E-4 19 8.388323453752377E-4 20 1.3980539089587294E-4 21 1.6776646907504754E-4 22 0.0031596018342467287 23 2.7961078179174586E-5 24 0.0 25 0.0016217425343921263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3576400.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.10954287157847 #Duplication Level Percentage of deduplicated Percentage of total 1 89.50965177466917 69.91557982713582 2 7.986876994924956 12.477026220902292 3 1.3604691221933918 3.1879686367627036 4 0.3885403991006738 1.2139485184357715 5 0.19904764827901783 0.7773760408368408 6 0.11804275751256932 0.5532159497164425 7 0.07528168572264685 0.41161526408784466 8 0.06081209581290163 0.3800004004006702 9 0.04158932521731437 0.2923670862955653 >10 0.22660949085484572 3.2849316090252536 >50 0.01742470034269232 0.9536059683785849 >100 0.013175817641338305 1.941243935245561 >500 8.61978353159598E-4 0.4491387685678273 >1k 0.0013288832640700491 2.039780091418862 >5k 1.7957881946892531E-4 0.9624361930450673 >10k+ 1.0774729168135519E-4 1.159765489744837 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 14469 0.40456884017447714 No Hit GTACATGGGAAGCAGTGGTATCAAC 13657 0.38186444469298736 No Hit GTATCAACGCAGAGTACTTTTTTTT 13215 0.3695056481377922 No Hit GGTATCAACGCAGAGTACTTTTTTT 9068 0.2535510569287552 No Hit TATCAACGCAGAGTACTTTTTTTTT 7911 0.22120008947545017 No Hit GAGTACATGGGAAGCAGTGGTATCA 6729 0.1881500950676658 No Hit CATGTACTCTGCGTTGATACCACTG 5491 0.15353428028184768 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5108 0.1428251873392238 No Hit ATTCCATTCCATTCCATTCCATTCC 4675 0.13071804048764118 No Hit GCGTTGATACCACTGCTTCCCATGT 4549 0.1271949446370652 No Hit GAATGGAATGGAATGGAATGGAATG 4063 0.11360586064198634 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4000 0.11184431271669835 No Hit GTATCAACGCAGAGTACATGGGAAG 3939 0.11013868694776871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.796107817917459E-5 0.0 4 0.0 0.0 0.0 5.592215635834918E-5 0.0 5 0.0 0.0 0.0 5.592215635834918E-5 0.0 6 2.796107817917459E-5 0.0 0.0 5.592215635834918E-5 0.0 7 2.796107817917459E-5 0.0 0.0 5.592215635834918E-5 0.0 8 2.796107817917459E-5 0.0 0.0 5.592215635834918E-5 0.0 9 2.796107817917459E-5 0.0 0.0 8.388323453752377E-5 0.0 10 2.796107817917459E-5 0.0 0.0 8.388323453752377E-5 0.0 11 2.796107817917459E-5 0.0 0.0 1.1184431271669836E-4 0.0 12 2.796107817917459E-5 0.0 0.0 1.1184431271669836E-4 0.0 13 2.796107817917459E-5 0.0 0.0 1.3980539089587294E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 75 2.0367553E-4 11.423006 5 CGCCCTA 220 0.0 11.207305 16 AATACCG 60 0.005790639 11.105699 5 TCGTAGG 60 0.0058697443 11.085474 2 TGTCCCG 260 0.0 10.584115 11 TGGACCG 90 9.329445E-5 10.576857 5 GTGCGCC 90 9.6324635E-5 10.543563 11 CGTGAAA 110 5.910293E-6 10.384113 10 GTCCCGC 260 0.0 10.212716 12 TCGCGCG 170 1.5097612E-9 10.078697 9 CAATCCG 95 1.6127338E-4 10.019758 10 TATTCCG 155 3.943933E-8 9.8262415 5 CGCGCGC 205 4.0017767E-11 9.750936 10 CGCGCCT 225 3.6379788E-12 9.693975 12 CTAGAGC 305 0.0 9.657603 3 TAGACAG 710 0.0 9.653243 5 ACTGCGT 90 0.0010939008 9.519171 8 CGAACCG 90 0.0011324023 9.483236 19 GCGCGCC 265 0.0 9.310164 11 TATCGCG 185 6.4792403E-9 9.261896 7 >>END_MODULE