FastQCFastQC Report
Wed 25 May 2016
SRR1294666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612213
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC630111.744387720214727No Hit
CCCATGTACTCTGCGTTGATACCAC600491.6623881260601188No Hit
GAGTACATGGGAAGCAGTGGTATCA280320.776033971418629No Hit
CATGTACTCTGCGTTGATACCACTG227390.6295032989472105No Hit
GCGTTGATACCACTGCTTCCCATGT191540.5302566598370583No Hit
GTATCAACGCAGAGTACATGGGAAG168070.46528263975573975No Hit
GTATCAACGCAGAGTACTTTTTTTT158510.4388168693263659No Hit
ACGCAGAGTACATGGGAAGCAGTGG146470.40548550154711255No Hit
TATCAACGCAGAGTACATGGGAAGC137000.3792688858602746No Hit
GTACTGGTTCACTATCGGTCAGTCA131500.36404276270530006No Hit
ACTCTGCGTTGATACCACTGCTTCC126180.3493149490353974No Hit
GGTATCAACGCAGAGTACATGGGAA117080.324122636178985No Hit
ATTCCATTCCATTCCATTCCATTCC113970.31551295563135395No Hit
GCTTCCCATGTACTCTGCGTTGATA97990.27127414690108254No Hit
GTTAATGATAGTGTGTCGAAACACA96900.2682566061303694No Hit
GGTATCAACGCAGAGTACTTTTTTT93890.259923764185556No Hit
TATCAACGCAGAGTACTTTTTTTTT91350.25289206367398603No Hit
GGATACCACGTGTCCCGCCCTACTC89070.24658014352974203No Hit
GAATGGAATGGAATGGAATGGAATG84190.23307041971223735No Hit
GTGGTATCAACGCAGAGTACATGGG78760.21803808357923524No Hit
CAGTGGTATCAACGCAGAGTACATG77610.21485443964683143No Hit
ATACCACTGCTTCCCATGTACTCTG69080.19124010682648004No Hit
ATACAGGGTGACAGCCCCGTACACA68810.19049264259887222No Hit
GTACATGGGGGTTAAGCGACTAAGC64720.17916994374362752No Hit
GTACTCTGCGTTGATACCACTGCTT64600.17883773742024625No Hit
AGCGTACACGGTGGATGCCCTGGCA64400.17828406021461082No Hit
GCAGTGGTATCAACGCAGAGTACAT60180.1666014711757031No Hit
ACTTAGATGTTTCAGTTCCCCCGGT59820.1656048522055593No Hit
GGGAAGCAGTGGTATCAACGCAGAG58800.16278109845681857No Hit
CATGGGAAGCAGTGGTATCAACGCA57660.15962513838469658No Hit
GTGGATGCCCTGGCAGTCAGAGGCG56900.15752116500328192No Hit
CTGCTTCCCATGTACTCTGCGTTGA54380.1505448322122754No Hit
ACCCTGTATCGCGCGCCTTTCCAGA53830.14902221989677797No Hit
TCATTAACCTATGGATTCAGTTAAT53580.14833012338973367No Hit
GCAGAGTACATGGGAAGCAGTGGTA52590.14558942122183824No Hit
TACCACTGCTTCCCATGTACTCTGC52130.14431596364887672No Hit
GTATCAACGCAGAGTACATGGGGGT50870.1408277972533735No Hit
ACATGGGAAGCAGTGGTATCAACGC49490.13700742453448897No Hit
GTTCACTATCGGTCAGTCAGGAGTA49490.13700742453448897No Hit
GTTGATACCACTGCTTCCCATGTAC47960.13277179391137786No Hit
ATCATTAACTGAATCCATAGGTTAA47800.1323288521468695No Hit
GTATTTAGCCTTGGAGGATGGTCCC47370.13113844615475334No Hit
GTACATGGTAAGCAGTGGTATCAAC47120.13044634964770904No Hit
GTACTTAGATGTTTCAGTTCCCCCG47060.1302802464860184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA46810.1295881499789741No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46730.12936667909671992No Hit
AAGCAGTGGTATCAACGCAGAGTAC45940.12717965413445995No Hit
CCATGTACTCTGCGTTGATACCACT45210.12515873233389063No Hit
GATACCACTGCTTCCCATGTACTCT44660.12363612001839315No Hit
CTATCGGTCAGTCAGGAGTATTTAG43970.12172593365895089No Hit
CCACTGCTTCCCATGTACTCTGCGT43340.11998185046119926No Hit
CCCATATTCAGACAGGATACCACGT43220.119649644137818No Hit
CCTATGGATTCAGTTAATGATAGTG42120.11660441950682311No Hit
CTTCCCATGTACTCTGCGTTGATAC41810.11574621983808818No Hit
GCCCAGAGCCTGAATCAGTGTGTGT41390.11458349770625376No Hit
TATTCAGACAGGATACCACGTGTCC41120.11383603347864592No Hit
GGTATCAACGCAGAGTACATGGGGG40060.1109015442887781No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39950.11059702182567861No Hit
GCCTTGGAGGATGGTCCCCCCATAT39440.10918514495130824No Hit
GTCAGGAGTATTTAGCCTTGGAGGA38860.10757948105496547No Hit
ATGGGAAGCAGTGGTATCAACGCAG38720.10719190701102066No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG36130.10002178719804176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA400.005281787414.2496371
GTCTGCG655.4733235E-513.1495019
ATCGCCG3500.013.02643419
AATCGCC3700.012.07495618
AAATCGC3800.012.00601617
GACCCGA802.8781276E-511.8712436
CCGGGGG7850.011.502418516
CGGAACA752.0813727E-411.3963934
ACCGGGG7950.011.35757715
AGACCCG855.3451033E-511.172785
CACTTCG600.005892682411.0796729
CCCCTAT1303.27218E-810.9612591
GTACAAA6900.010.87653351
GTACTGG30900.010.72951
CGGGGGA8150.010.612679517
TACTGGT28900.010.5815732
CCGTACA17400.010.54263917
CTCGGAA1451.4353645E-810.48757812
TGTCCCG18300.010.4824411
CCCGTAC17450.010.40334916
Produced by FastQC (version 0.11.5)