FastQCFastQC Report
Wed 25 May 2016
SRR1294666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612213
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC676861.8738097670320106No Hit
GTACATGGGAAGCAGTGGTATCAAC605881.6773097267519939No Hit
GTATCAACGCAGAGTACTTTTTTTT435911.2067671535427176No Hit
GAGTACATGGGAAGCAGTGGTATCA298820.827249112939907No Hit
GGTATCAACGCAGAGTACTTTTTTT282100.7809616985487844No Hit
TATCAACGCAGAGTACTTTTTTTTT267250.7398511660303532No Hit
CATGTACTCTGCGTTGATACCACTG262890.7277810029475006No Hit
GCGTTGATACCACTGCTTCCCATGT207650.5748553587509928No Hit
ACGCAGAGTACATGGGAAGCAGTGG176360.4882325599293287No Hit
ACGCAGAGTACTTTTTTTTTTTTTT171900.47588555824365836No Hit
GTATCAACGCAGAGTACATGGGAAG169190.4683832321072982No Hit
TATCAACGCAGAGTACATGGGAAGC145920.40396288923161505No Hit
ACTCTGCGTTGATACCACTGCTTCC144210.3992289491234321No Hit
GTACTGGTTCACTATCGGTCAGTCA124020.3433352352145347No Hit
GGTATCAACGCAGAGTACATGGGAA123950.3431414481925623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT111610.3089795646048558No Hit
ATTCCATTCCATTCCATTCCATTCC110020.3045778308200541No Hit
GCTTCCCATGTACTCTGCGTTGATA108570.3005636710791972No Hit
GTTAATGATAGTGTGTCGAAACACA96440.2669831485574079No Hit
GAGTACTTTTTTTTTTTTTTTTTTT95430.2641870786689489No Hit
CAGTGGTATCAACGCAGAGTACATG92270.255438978819909No Hit
GGATACCACGTGTCCCGCCCTACTC92110.2549960370554007No Hit
GAATGGAATGGAATGGAATGGAATG91690.2538333149235662No Hit
GTGGTATCAACGCAGAGTACATGGG84840.2348698706305525No Hit
ATACAGGGTGACAGCCCCGTACACA76740.2124459438023173No Hit
ATACCACTGCTTCCCATGTACTCTG75330.20854251950258748No Hit
CATGGGAAGCAGTGGTATCAACGCA74360.20585718505525558No Hit
AGCGTACACGGTGGATGCCCTGGCA74260.20558034645243786No Hit
GCAGTGGTATCAACGCAGAGTACAT69780.1931779770462041No Hit
GGGAAGCAGTGGTATCAACGCAGAG66030.18279652944053962No Hit
ACTTAGATGTTTCAGTTCCCCCGGT65690.1818552781909594No Hit
GTACTCTGCGTTGATACCACTGCTT61820.17114162426191368No Hit
ACATGGGAAGCAGTGGTATCAACGC60530.1675704062855651No Hit
GCAGAGTACATGGGAAGCAGTGGTA59730.16535569746302337No Hit
ACCCTGTATCGCGCGCCTTTCCAGA59340.16427602691203427No Hit
CTGCTTCCCATGTACTCTGCGTTGA59340.16427602691203427No Hit
GTGGATGCCCTGGCAGTCAGAGGCG57540.15929293206131534No Hit
TACCACTGCTTCCCATGTACTCTGC56880.15746579728271837No Hit
GTACATGGGGGTTAAGCGACTAAGC56260.1557493979452485No Hit
TCATTAACCTATGGATTCAGTTAAT56240.15569403022468498No Hit
GCAGAGTACTTTTTTTTTTTTTTTT55300.15309174735819844No Hit
CCATGTACTCTGCGTTGATACCACT53330.14763802688268937No Hit
AAGCAGTGGTATCAACGCAGAGTAC52620.14567247280268356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA50700.14035717162858335No Hit
CTATCGGTCAGTCAGGAGTATTTAG49480.1369797406742072No Hit
GATACCACTGCTTCCCATGTACTCT49270.13639837960829No Hit
CTTCCCATGTACTCTGCGTTGATAC49000.13565091538068216No Hit
CCACTGCTTCCCATGTACTCTGCGT48940.13548481221899153No Hit
GTATCAACGCAGAGTACATGGGGGT48860.13526334133673734No Hit
CCCATATTCAGACAGGATACCACGT48840.13520797361617382No Hit
GTTGATACCACTGCTTCCCATGTAC48200.1334362065581404No Hit
ATGGGAAGCAGTGGTATCAACGCAG48180.13338083883757684No Hit
ATCATTAACTGAATCCATAGGTTAA47760.13221811670574243No Hit
CCTATGGATTCAGTTAATGATAGTG46340.12828700854573083No Hit
GTACATGGTAAGCAGTGGTATCAAC45260.12529715163529948No Hit
GTACTTAGATGTTTCAGTTCCCCCG44310.12266718490853114No Hit
ATCAACGCAGAGTACTTTTTTTTTT44260.12252876560712228No Hit
GTTCACTATCGGTCAGTCAGGAGTA44200.12236266244543166No Hit
GGTATCAACGCAGAGTACATGGGGG43550.12056321152711649No Hit
GTCAGGAGTATTTAGCCTTGGAGGA43280.11981574729950865No Hit
GCCTTGGAGGATGGTCCCCCCATAT42860.11865302516767423No Hit
GCCCAGAGCCTGAATCAGTGTGTGT42810.11851460586626537No Hit
TATTCAGACAGGATACCACGTGTCC42050.1164106324848507No Hit
GTATTTAGCCTTGGAGGATGGTCCC41590.11513717491188918No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG38330.10611223646003157No Hit
ACCATGTACTCTGCGTTGATACCAC37730.10445120484312526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG453.4704673E-516.9285438
AGGACCG1151.8189894E-1214.9044785
GACGGAC456.6461565E-414.8124757
CGCTAAT400.00534884814.2225614
AATCGCC4100.013.875669518
ATCGCCG4100.013.41333219
GGACCGT1351.8189894E-1213.4017636
GACCGTT1259.822543E-1112.95033557
AAATCGC4400.012.714107517
CTTCACG604.0709932E-412.6752621
CCGTTCA701.0681688E-412.2427949
GCACCCG550.003114427412.06762818
ACCGTTC1052.641882E-711.789528
TCGCTCC1156.8584995E-811.5922119
CGCTCCA1406.548362E-1011.56263810
TAGGACC1655.456968E-1211.5421894
GAAATCG4900.011.4167516
CCGAACA1501.8189894E-1011.37804917
ACCCCGT3050.011.19152414
GTCCTAT1451.20599E-911.1454891
Produced by FastQC (version 0.11.5)