FastQCFastQC Report
Wed 25 May 2016
SRR1294665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907216
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99130.5197628375600876No Hit
GTACATGGGAAGCAGTGGTATCAAC70700.37069739347824265No Hit
GGTATCAACGCAGAGTACTTTTTTT65160.34164981837400693No Hit
CCCATGTACTCTGCGTTGATACCAC61030.31999521816092147No Hit
TATCAACGCAGAGTACTTTTTTTTT55590.291471967517051No Hit
GAGTACATGGGAAGCAGTGGTATCA32820.1720832878918801No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27740.1454476053053246No Hit
ATTCCATTCCATTCCATTCCATTCC25470.1335454400550331No Hit
CATGTACTCTGCGTTGATACCACTG24090.12630976250199244No Hit
GGTATCAACGCAGAGTACATGGGAA23090.12106651789833979No Hit
GTATCAACGCAGAGTACATGGGAAG21320.11178597494987458No Hit
GTGGTATCAACGCAGAGTACATGGG20740.10874489307975606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA1103.8331564E-812.0869032
TAATACG550.003077116612.0865854
GCGGAGT550.003077721212.0862678
CTAGACT2300.010.735294
TCTATAC2550.010.4281893
GTTGTAG1901.0004442E-1010.0000961
GTGTAGG1901.0004442E-1010.0000961
TAGACTG1901.0004442E-109.9964235
CTAGTAC1151.0302594E-59.9100183
CTTGGAC1356.470036E-79.8488453
CTTAGCC1554.0825398E-89.8034583
GTCTAGA2054.1836756E-119.7318011
CCTAGAC1901.0568328E-99.4971013
ACGGTAC900.00111687039.4971013
CTAGCCC800.00452958479.4966034
TATTCCG1106.86367E-59.4966025
TAGGACT2106.730261E-119.4966024
CGGTCAG2252.7284841E-119.29385816
TTAGACA2252.7284841E-119.2855664
CAGGACC1752.6846465E-89.225274
Produced by FastQC (version 0.11.5)