##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294665_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907216 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.055112268353454 31.0 30.0 33.0 25.0 34.0 2 30.031151689163682 31.0 30.0 34.0 25.0 34.0 3 30.106610368201608 31.0 30.0 34.0 25.0 34.0 4 33.512004932844526 35.0 35.0 37.0 27.0 37.0 5 34.02156913532605 35.0 35.0 37.0 28.0 37.0 6 34.06709989849078 37.0 35.0 37.0 29.0 37.0 7 34.008820710396726 37.0 35.0 37.0 29.0 37.0 8 33.91014756587612 37.0 35.0 37.0 28.0 37.0 9 34.89639820555197 39.0 35.0 39.0 26.0 39.0 10 35.09203519685238 39.0 35.0 39.0 27.0 39.0 11 35.274840395634264 39.0 35.0 39.0 27.0 39.0 12 35.0975274955747 39.0 35.0 39.0 27.0 39.0 13 34.923213731428426 39.0 35.0 39.0 27.0 39.0 14 35.823205132507276 39.0 35.0 41.0 26.0 41.0 15 35.84161888323084 39.0 35.0 41.0 27.0 41.0 16 35.98323944429996 39.0 35.0 41.0 27.0 41.0 17 35.99318063606849 39.0 35.0 41.0 27.0 41.0 18 35.90247198010084 39.0 35.0 41.0 27.0 41.0 19 36.21317931477085 39.0 36.0 41.0 27.0 41.0 20 36.34547424098791 39.0 36.0 41.0 27.0 41.0 21 36.418032357111095 40.0 36.0 41.0 27.0 41.0 22 36.44136217397505 39.0 36.0 41.0 27.0 41.0 23 36.44726082415416 39.0 36.0 41.0 27.0 41.0 24 36.534071652083455 39.0 36.0 41.0 28.0 41.0 25 35.42275704482345 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.010598543625619 1101 2 0.9957352991493948 1101 3 1.241210014589445 1101 4 1.0515059266536113 1101 5 0.7199130283026705 1101 6 0.8090845030266323 1101 7 0.8438240574282929 1101 8 0.735376241478761 1101 9 1.08425154163087 1101 10 1.022986362496681 1101 11 0.8153713629603772 1101 12 1.103679797466846 1101 13 1.0317039180061087 1101 14 1.0968079414241458 1101 15 1.2639732411224145 1101 16 1.2043248273987928 1101 17 1.2041795451078983 1101 18 1.1797136481573531 1101 19 1.0960706102938644 1101 20 0.9535617530992511 1101 21 0.9219776309801659 1101 22 0.8670709220387849 1101 23 1.02709047329256 1101 24 0.9622494311697807 1101 25 1.0130787582991516 1102 1 0.9022048487657592 1102 2 0.8820000108370394 1102 3 1.1297451688670783 1102 4 1.1059701549900183 1102 5 0.8837532607519236 1102 6 0.7885294523963893 1102 7 0.6875215566709088 1102 8 0.6935285476103914 1102 9 1.1045165470932474 1102 10 0.9635778825054899 1102 11 0.7510980996739605 1102 12 1.0130786953525899 1102 13 1.0749421647217972 1102 14 1.1530333836928222 1102 15 1.35360264889092 1102 16 1.1637965378103559 1102 17 1.1926795841248534 1102 18 1.3111000927486813 1102 19 1.017861121370089 1102 20 0.3088722477424497 1102 21 0.5374919203666408 1102 22 0.5718502696752807 1102 23 0.6841483537996851 1102 24 0.7179763170658404 1102 25 0.7479525154943332 1103 1 0.6345093887682296 1103 2 0.6296749402304478 1103 3 0.9000971892282408 1103 4 0.7031893548767485 1103 5 0.544493296764621 1103 6 0.37676448197078827 1103 7 0.3015366244398905 1103 8 0.42620128061214757 1103 9 0.5196325380430693 1103 10 0.5193942293461973 1103 11 0.5177909663625613 1103 12 0.6032646545780409 1103 13 0.5629682034235515 1103 14 0.4764420442652977 1103 15 0.6868899943673696 1103 16 0.8030154173778783 1103 17 0.569810475720594 1103 18 0.8335749532038292 1103 19 0.6325564322334927 1103 20 -0.15776450505642003 1103 21 0.10808458633347584 1103 22 -0.5460148666547795 1103 23 0.1812383524708281 1103 24 0.3797498467455611 1103 25 0.3786127212297927 1104 1 0.3729266167287406 1104 2 0.35000806770619164 1104 3 0.7065097917394105 1104 4 0.5181791137899339 1104 5 0.45983717158220117 1104 6 0.24973495738218787 1104 7 0.3309035736902288 1104 8 0.42073306985307113 1104 9 0.5616636682041474 1104 10 0.7320368032342657 1104 11 0.3922887416854124 1104 12 0.40325664495248503 1104 13 0.5012932842164588 1104 14 0.7097126865949548 1104 15 0.7162008470598522 1104 16 0.617300504315871 1104 17 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0.40113137818437394 2207 5 0.2725101937751191 2207 6 0.30154912077993146 2207 7 0.2603340453874736 2207 8 0.32522284438829274 2207 9 -1.3407604251680496 2207 10 -0.209111553059671 2207 11 0.16000962019967346 2207 12 0.3746827632590737 2207 13 0.6441757898601992 2207 14 0.8349824000807047 2207 15 0.8033711507570445 2207 16 0.8076464159975174 2207 17 0.7421235503584853 2207 18 0.8617342113124451 2207 19 -0.43227713254579925 2207 20 0.14868657791518558 2207 21 -0.23512793345796013 2207 22 0.20660725175987693 2207 23 -0.5027938312020268 2207 24 -1.5043533303232834 2207 25 -0.37037634029740474 2208 1 0.41115158535513174 2208 2 0.10252987037810968 2208 3 0.5925116026717241 2208 4 0.5046400119488226 2208 5 0.35135279484473614 2208 6 0.18490040352283899 2208 7 0.19082795932349939 2208 8 0.32575443567728257 2208 9 -0.2881973347981841 2208 10 0.04379674350526841 2208 11 0.09788674648279283 2208 12 0.1999477221395054 2208 13 0.34391373504328726 2208 14 0.6543569337077884 2208 15 0.3858470798440763 2208 16 0.2710248295847322 2208 17 0.17168711391022384 2208 18 0.6259157215173516 2208 19 -1.4885092528665638 2208 20 -0.2461730655773806 2208 21 -0.4021283213227136 2208 22 -0.029211894320752663 2208 23 0.08598217657364415 2208 24 0.29897233103206844 2208 25 0.3933959102435409 2209 1 0.21746152678755237 2209 2 -1.4576856782109857 2209 3 -0.13320131211104425 2209 4 0.16695972533622694 2209 5 0.21003348927672505 2209 6 0.29050454901027933 2209 7 0.3168659513333836 2209 8 0.3338028136623663 2209 9 -1.8444674434692914 2209 10 -0.27170700509469015 2209 11 0.12244382385130592 2209 12 0.34857301728488466 2209 13 0.5134784942105526 2209 14 0.6559259582862751 2209 15 0.9017115816858094 2209 16 0.7810097278295345 2209 17 0.5080650623364491 2209 18 0.6577511640995937 2209 19 0.4265428177496915 2209 20 0.4339337268085046 2209 21 0.4166466887091218 2209 22 0.2825748717761627 2209 23 0.4272950015445005 2209 24 0.5238019207840807 2209 25 0.39709220720413896 2210 1 0.13562472255945934 2210 2 0.19012759240195365 2210 3 0.032551855800708296 2210 4 0.1895805129162298 2210 5 0.1575225664732045 2210 6 0.13938824479541978 2210 7 0.11338013435798189 2210 8 0.1400167881830825 2210 9 0.2635642178564268 2210 10 -1.2921342865645187 2210 11 -0.46172390245543227 2210 12 -2.2866477616203795 2210 13 -0.7547747728928798 2210 14 -0.4530890375820036 2210 15 -0.5841532565881664 2210 16 -0.16136594372787982 2210 17 -0.05077481812122642 2210 18 0.24912743569242934 2210 19 0.1942848763247369 2210 20 0.22195941220654447 2210 21 0.2270936560149437 2210 22 0.206965522895473 2210 23 0.01597992709528029 2210 24 0.06670544394730626 2210 25 -0.00720874426362883 2211 1 -0.3762943161247776 2211 2 -0.07264820281601558 2211 3 -1.6206105259046275 2211 4 -0.7270163876568034 2211 5 -0.4843964515992596 2211 6 -0.4478444854399726 2211 7 -0.39988094359621584 2211 8 -0.2180088973483052 2211 9 0.08485351516787887 2211 10 0.06380648317695403 2211 11 -0.29575660252657343 2211 12 -0.09969341817730992 2211 13 -0.208069965454051 2211 14 -0.7006003266113652 2211 15 -0.3173206391346781 2211 16 -0.06381578019482959 2211 17 -0.16685894057133055 2211 18 0.0977703960312013 2211 19 -0.12546418891385258 2211 20 0.0022703840705560197 2211 21 -0.161927720733118 2211 22 -0.0918185320972924 2211 23 -0.48707861039464717 2211 24 -0.24893001008337023 2211 25 0.023233556541228495 2212 1 -0.30464625582970584 2212 2 -0.1448905747001632 2212 3 -2.1287156550072766 2212 4 -1.2079410709512857 2212 5 -0.5379917388126287 2212 6 -0.37800319195579846 2212 7 -0.2650258349742316 2212 8 -0.30252626122637594 2212 9 -0.8342560601004152 2212 10 -0.2031347484928574 2212 11 -0.26104374141525 2212 12 0.006890928638505045 2212 13 -0.3054596119205826 2212 14 -0.0629099495437444 2212 15 0.04745262131231698 2212 16 -1.069720169014623 2212 17 -1.1130625704894825 2212 18 -0.22277803887380543 2212 19 -0.13584602293855852 2212 20 -0.09870896397409723 2212 21 0.09316004821521773 2212 22 -0.13944389237076393 2212 23 -0.019647332326826472 2212 24 -0.08389757616143356 2212 25 -0.040329482938041394 2213 1 -2.8392994006991223 2213 2 -1.1673402140289895 2213 3 -2.2583960930786766 2213 4 -0.8956531123694234 2213 5 -0.7924660042539529 2213 6 -0.6399006168363428 2213 7 -0.21542823974993297 2213 8 -0.014035658064891265 2213 9 -0.04036305010940566 2213 10 0.06103650058651766 2213 11 -0.21608071221282188 2213 12 -0.08963495420589851 2213 13 -0.38148690142568853 2213 14 -0.012580208887698063 2213 15 -0.008923727071262988 2213 16 -1.3786608132319529 2213 17 -0.8591913063350773 2213 18 -0.033980307604537074 2213 19 0.08993647186835574 2213 20 -0.0947412319366947 2213 21 0.15725390939100947 2213 22 0.1548722454306386 2213 23 0.23668153175923123 2213 24 0.13638840582492406 2213 25 0.30208011513366984 2214 1 -1.1147130660609896 2214 2 -0.837865128201031 2214 3 -6.29084126746184 2214 4 -2.1663537599560776 2214 5 -1.1871382537486141 2214 6 -0.6964354399933157 2214 7 -0.6864465978423624 2214 8 -0.2820739009713833 2214 9 -0.26443969768536846 2214 10 -0.22487688536656947 2214 11 -0.6677571982931809 2214 12 -0.33484406293201374 2214 13 -5.2932159537887635 2214 14 -3.599105520114321 2214 15 -1.897128966854794 2214 16 -2.8217613549373866 2214 17 -3.0908703124419645 2214 18 -1.1266877051190036 2214 19 -0.634609589136339 2214 20 -0.30409494205744636 2214 21 -0.35206083055829396 2214 22 -0.29768157083870506 2214 23 -0.20354521046812835 2214 24 -0.007568944898594054 2214 25 0.01014072411111755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 741.0 3 111.0 4 88.0 5 224.0 6 22.0 7 24.0 8 53.0 9 133.0 10 379.0 11 966.0 12 2208.0 13 4380.0 14 7398.0 15 10942.0 16 14212.0 17 16095.0 18 17297.0 19 17029.0 20 16530.0 21 15683.0 22 15320.0 23 14974.0 24 14904.0 25 15736.0 26 16471.0 27 18730.0 28 21504.0 29 26560.0 30 33606.0 31 42298.0 32 57086.0 33 76575.0 34 105252.0 35 148082.0 36 211827.0 37 326617.0 38 637159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98471357303501 20.93849476587492 12.573919387388239 21.502872273701833 2 14.70588698182604 22.332727762042484 41.31190558753618 21.649479668595298 3 18.541448510170376 29.459835771439842 28.987725877225195 23.010989841164584 4 11.264811248548842 18.093133225301997 38.336366359482064 32.30568916666711 5 11.653812701766682 41.31263619414925 33.652138241585995 13.38141286249807 6 28.849233332913588 39.396624817934935 18.27214477898245 13.481997070169033 7 25.2485462881883 35.54365385210676 22.489130280805234 16.718669578899707 8 24.4553723530674 38.145734556750774 20.78569715958903 16.6131959305928 9 25.374947204390498 18.120607470873875 21.503566490987364 35.00087883374827 10 14.857358424832798 32.225353383726905 32.30757042096487 20.609717770475427 11 30.902410706181772 28.42948124356322 21.77604970307533 18.892058347179674 12 20.265838424591234 30.704574131250833 30.930749274020286 18.09883817013764 13 25.86135253990789 26.1136067206222 26.701692642341555 21.323348097128353 14 20.01588683203033 24.97595726295278 28.47504775755897 26.533108147457924 15 22.881258555530078 31.373276367297663 24.618834574808844 21.126630502363412 16 20.50249289486925 32.88260283092963 26.214509104588256 20.40039516961286 17 18.625512274385468 31.778037430986256 28.779707454664603 20.816742839963673 18 19.01532987908739 30.862329326716083 30.392026110570875 19.730314683625654 19 19.828844569671595 29.71320184482677 31.10589914317346 19.352054442328175 20 22.123784230170713 28.57527358428201 29.962266407974003 19.33867577757328 21 22.430218392481077 29.374171702470807 28.818458167761268 19.377151737286844 22 20.74171780975872 28.93940848731352 30.42146944767559 19.89740425525217 23 20.25677820480869 29.55242205228432 30.075317759093355 20.115481983813634 24 20.029309495363528 28.987489683173067 30.33910550946105 20.64409531200236 25 20.817091938059093 28.80772724005329 29.298786230619854 21.076394591267768 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2225.0 1 2225.0 2 2544.5 3 2864.0 4 2864.0 5 2864.0 6 4457.0 7 6050.0 8 6050.0 9 6050.0 10 9001.5 11 11953.0 12 11953.0 13 11953.0 14 17810.5 15 23668.0 16 23668.0 17 23668.0 18 35131.5 19 46595.0 20 46595.0 21 46595.0 22 63858.5 23 81122.0 24 81122.0 25 81122.0 26 103610.5 27 126099.0 28 126099.0 29 126099.0 30 148934.0 31 171769.0 32 171769.0 33 171769.0 34 188664.5 35 205560.0 36 205560.0 37 205560.0 38 214973.5 39 224387.0 40 224387.0 41 224387.0 42 223736.5 43 223086.0 44 223086.0 45 223086.0 46 230088.5 47 237091.0 48 237091.0 49 237091.0 50 219338.5 51 201586.0 52 201586.0 53 201586.0 54 174119.5 55 146653.0 56 146653.0 57 146653.0 58 122044.5 59 97436.0 60 97436.0 61 97436.0 62 77166.5 63 56897.0 64 56897.0 65 56897.0 66 41433.5 67 25970.0 68 25970.0 69 25970.0 70 18243.0 71 10516.0 72 10516.0 73 10516.0 74 7166.5 75 3817.0 76 3817.0 77 3817.0 78 2543.0 79 1269.0 80 1269.0 81 1269.0 82 844.0 83 419.0 84 419.0 85 419.0 86 277.0 87 135.0 88 135.0 89 135.0 90 84.0 91 33.0 92 33.0 93 33.0 94 21.0 95 9.0 96 9.0 97 9.0 98 8.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10124705329653273 2 0.053848122079512756 3 0.05725623107188698 4 0.05133136466975948 5 0.04876217481396968 6 0.06837190963163062 7 0.06464920596303722 8 0.062342178337430054 9 0.0671659633727905 10 0.0673756931569366 11 0.06171298898499174 12 0.06139839430877258 13 0.0630238001359049 14 0.06475407085511027 15 0.0673756931569366 16 0.06286650279779532 17 0.06580271977584082 18 0.14156760429862167 19 0.07057407236516472 20 0.06407244905663544 21 0.06281407035175879 22 0.0675854229410827 23 0.06769028783315575 24 0.0707313697032743 25 0.06999731545876292 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1907216.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.1583784591731 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40149067144517 73.26595280974281 2 6.614954878179328 10.604881132309037 3 1.0338765628337292 2.4862160641108537 4 0.3246167162494127 1.0408299838117772 5 0.15814310279155308 0.6338247342136611 6 0.09720450869885919 0.46750534777326824 7 0.0668541528131531 0.3751244337934883 8 0.04182045028547584 0.2681807585052965 9 0.029995952233175048 0.21639842022151112 >10 0.19680584164768625 3.0384689408475456 >50 0.01820125016947214 1.0028279882327458 >100 0.012882345640813364 2.189747581303809 >500 0.001773881444385596 0.9832276152598092 >1k 0.0010511890040803497 1.5751127340549305 >5k 3.2849656377510923E-4 1.8517014558194995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9913 0.5197628375600876 No Hit GTACATGGGAAGCAGTGGTATCAAC 7070 0.37069739347824265 No Hit GGTATCAACGCAGAGTACTTTTTTT 6516 0.34164981837400693 No Hit CCCATGTACTCTGCGTTGATACCAC 6103 0.31999521816092147 No Hit TATCAACGCAGAGTACTTTTTTTTT 5559 0.291471967517051 No Hit GAGTACATGGGAAGCAGTGGTATCA 3282 0.1720832878918801 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2774 0.1454476053053246 No Hit ATTCCATTCCATTCCATTCCATTCC 2547 0.1335454400550331 No Hit CATGTACTCTGCGTTGATACCACTG 2409 0.12630976250199244 No Hit GGTATCAACGCAGAGTACATGGGAA 2309 0.12106651789833979 No Hit GTATCAACGCAGAGTACATGGGAAG 2132 0.11178597494987458 No Hit GTGGTATCAACGCAGAGTACATGGG 2074 0.10874489307975606 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.2432446036526536E-5 0.0 10 0.0 0.0 0.0 1.0486489207305307E-4 0.0 11 0.0 0.0 0.0 1.0486489207305307E-4 0.0 12 0.0 0.0 0.0 1.0486489207305307E-4 0.0 13 0.0 0.0 0.0 1.0486489207305307E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 110 3.8331564E-8 12.086903 2 TAATACG 55 0.0030771166 12.086585 4 GCGGAGT 55 0.0030777212 12.086267 8 CTAGACT 230 0.0 10.73529 4 TCTATAC 255 0.0 10.428189 3 GTTGTAG 190 1.0004442E-10 10.000096 1 GTGTAGG 190 1.0004442E-10 10.000096 1 TAGACTG 190 1.0004442E-10 9.996423 5 CTAGTAC 115 1.0302594E-5 9.910018 3 CTTGGAC 135 6.470036E-7 9.848845 3 CTTAGCC 155 4.0825398E-8 9.803458 3 GTCTAGA 205 4.1836756E-11 9.731801 1 CCTAGAC 190 1.0568328E-9 9.497101 3 ACGGTAC 90 0.0011168703 9.497101 3 CTAGCCC 80 0.0045295847 9.496603 4 TATTCCG 110 6.86367E-5 9.496602 5 TAGGACT 210 6.730261E-11 9.496602 4 CGGTCAG 225 2.7284841E-11 9.293858 16 TTAGACA 225 2.7284841E-11 9.285566 4 CAGGACC 175 2.6846465E-8 9.22527 4 >>END_MODULE