FastQCFastQC Report
Wed 25 May 2016
SRR1294665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907216
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT266471.3971673895353227No Hit
GGTATCAACGCAGAGTACTTTTTTT190100.9967407991543695No Hit
TATCAACGCAGAGTACTTTTTTTTT164560.8628283319770808No Hit
ACGCAGAGTACTTTTTTTTTTTTTT101170.530459056551539No Hit
GTACATGGGAAGCAGTGGTATCAAC72850.3819703693760958No Hit
CCCATGTACTCTGCGTTGATACCAC65920.34563468427278293No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47660.24989303781008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43490.2280287078128539No Hit
GAGTACATGGGAAGCAGTGGTATCA36330.19048707645070093No Hit
GTGGTATCAACGCAGAGTACTTTTT27450.14392706437026537No Hit
CATGTACTCTGCGTTGATACCACTG26780.14041409048581807No Hit
ATCAACGCAGAGTACTTTTTTTTTT26220.13747787350777257No Hit
GGTATCAACGCAGAGTACATGGGAA25030.13123841242942594No Hit
GTGGTATCAACGCAGAGTACATGGG23310.12222003171114336No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23000.12059462588401104No Hit
ATTCCATTCCATTCCATTCCATTCC22690.11896922005687872No Hit
GTATCAACGCAGAGTACATGGGAAG22120.11598057063279671No Hit
ACGCAGAGTACATGGGAAGCAGTGG21140.11084219092121711No Hit
GCGTTGATACCACTGCTTCCCATGT20320.10654273034622191No Hit
GAATGGAATGGAATGGAATGGAATG19990.10481245962701655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCG551.9918408E-413.79238217
CCCGTAC751.507281E-512.64334916
GCGCCTT604.158725E-412.64268613
ATCCGAA550.003112744812.06801812
CAGCGTA657.8865956E-411.71655710
CGCAGCG752.0337521E-411.4242438
GAGTGCC752.0347469E-411.4236429
CCCCGTA855.4278447E-511.15531215
CCCCCGG700.001515105110.83687218
CCGTACA909.6987504E-510.53584817
CTAGGAC1553.6543497E-910.4209393
GGTATCA60850.010.2533711
TAGACAG2600.010.2525275
CGCCCTA856.707043E-410.04030716
TGTCCCG951.6636334E-49.988411
GTTAGCC1054.0986703E-59.9538963
ACGCAGC1002.6973922E-49.5202047
GTATCGC900.00109232119.5202046
CTCTAGG1804.100002E-99.5109411
CCTAGTA2401.8189894E-129.5014452
Produced by FastQC (version 0.11.5)