##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907216 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43517462101828 31.0 31.0 34.0 28.0 34.0 2 31.539018129042542 31.0 31.0 34.0 28.0 34.0 3 31.610093455591816 31.0 31.0 34.0 28.0 34.0 4 35.18329019890773 37.0 35.0 37.0 32.0 37.0 5 35.431178219981376 37.0 35.0 37.0 33.0 37.0 6 35.05858172330769 37.0 35.0 37.0 32.0 37.0 7 34.92389745052474 37.0 35.0 37.0 32.0 37.0 8 34.97106463033028 37.0 35.0 37.0 32.0 37.0 9 36.37123744767242 39.0 37.0 39.0 32.0 39.0 10 36.17867247338529 39.0 37.0 39.0 31.0 39.0 11 36.346904598115785 39.0 37.0 39.0 32.0 39.0 12 36.308455885437205 39.0 37.0 39.0 32.0 39.0 13 36.15399566698266 39.0 37.0 39.0 31.0 39.0 14 37.24546354476892 40.0 37.0 41.0 31.0 41.0 15 37.295468368553955 40.0 37.0 41.0 31.0 41.0 16 37.355576924690226 40.0 37.0 41.0 31.0 41.0 17 37.44972095452219 40.0 37.0 41.0 32.0 41.0 18 37.485878369308985 40.0 37.0 41.0 32.0 41.0 19 37.54089625925957 40.0 37.0 41.0 31.0 41.0 20 37.610109185325626 40.0 37.0 41.0 32.0 41.0 21 37.47354416070335 40.0 37.0 41.0 31.0 41.0 22 37.47783732938482 40.0 37.0 41.0 31.0 41.0 23 37.49554271776243 40.0 37.0 41.0 31.0 41.0 24 37.440251654768 40.0 37.0 41.0 31.0 41.0 25 36.49711254519677 39.0 35.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.533950790626136 1101 2 0.48743825966939625 1101 3 0.31916336191236994 1101 4 0.2899098475621642 1101 5 0.30844775376382216 1101 6 0.35441279471572784 1101 7 0.4640583415624988 1101 8 0.3962915456034395 1101 9 0.5942879859541392 1101 10 0.6761396618394571 1101 11 0.5323064305659599 1101 12 0.6832942092719421 1101 13 0.6738684637668868 1101 14 0.8924427350944555 1101 15 0.8233914164763689 1101 16 0.9652652212948567 1101 17 0.8196008935690102 1101 18 0.8398004330059123 1101 19 0.9006079261047759 1101 20 0.8609474378422988 1101 21 1.040307768112406 1101 22 0.9760208843612546 1101 23 0.962096356583352 1101 24 1.1084001092707183 1101 25 1.0922592487529172 1102 1 0.3724994860827273 1102 2 0.40174325292175794 1102 3 0.3810015081020204 1102 4 0.3524729632267807 1102 5 0.3705240181427172 1102 6 0.44405884206854296 1102 7 0.4777512850016805 1102 8 0.4298197342068164 1102 9 0.5731895668294058 1102 10 0.6198301609810883 1102 11 0.6203430284709839 1102 12 0.6553833148196944 1102 13 0.7412300912788865 1102 14 0.8939859130943972 1102 15 0.8389784036329004 1102 16 0.8051201241196893 1102 17 0.8270149759361018 1102 18 0.7242956109691363 1102 19 0.919186021985503 1102 20 0.782678562815903 1102 21 1.1110222718626233 1102 22 1.0486366040634394 1102 23 0.9696432687355241 1102 24 1.0967707611752004 1102 25 0.9696309632956783 1103 1 0.2579881266468611 1103 2 0.2607312821520438 1103 3 0.11937920158323223 1103 4 0.09268364273871299 1103 5 0.14630869864462426 1103 6 0.13952029031754876 1103 7 0.2124873785348882 1103 8 0.16949625823279035 1103 9 0.07908050974585734 1103 10 0.4212064310148165 1103 11 0.41652615240723634 1103 12 0.3213346309428289 1103 13 0.4103068408049353 1103 14 0.5325476160319909 1103 15 0.616826444666259 1103 16 0.6175616237938542 1103 17 0.45835861635225683 1103 18 0.4652207705919693 1103 19 0.6043054250311997 1103 20 0.5840176943910791 1103 21 0.8407712216856638 1103 22 0.786524389050328 1103 23 0.7124798983971985 1103 24 0.8248111968325347 1103 25 0.6969588047794559 1104 1 0.1389868292042351 1104 2 0.1951717674974205 1104 3 0.05275363348704687 1104 4 0.0807333251767588 1104 5 0.11159455838747334 1104 6 0.11840843465654416 1104 7 0.21597156565199782 1104 8 0.14894099130582106 1104 9 0.03144176632626028 1104 10 0.37082559447435415 1104 11 0.3420658136949086 1104 12 0.41191226208658804 1104 13 0.06854902999754131 1104 14 0.21072005874748356 1104 15 0.3924419639650054 1104 16 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0.1865274338809435 2207 4 0.20324431589085634 2207 5 0.11177958199461102 2207 6 0.2969351491895864 2207 7 0.14471197552155957 2207 8 0.18857505757517856 2207 9 0.2817435730921787 2207 10 0.453988993672624 2207 11 0.376573963776913 2207 12 0.3664331129651188 2207 13 0.4087187430744379 2207 14 0.3668171305860568 2207 15 0.450629948088455 2207 16 0.42818788369789473 2207 17 0.45309670395570123 2207 18 0.45262328983858424 2207 19 0.5890954954381868 2207 20 0.432264007787623 2207 21 0.8407934721056165 2207 22 0.6603626363993058 2207 23 0.6602357044051459 2207 24 0.8215522381272322 2207 25 0.6769675302712415 2208 1 0.05117090888525766 2208 2 0.15778713500548136 2208 3 0.03866013219238695 2208 4 0.0588162785849633 2208 5 0.004195152500351185 2208 6 0.15848246048616232 2208 7 0.13310234991277525 2208 8 -0.0016443846214926339 2208 9 0.17358089040688895 2208 10 0.169152864891025 2208 11 -0.03670656482171353 2208 12 -0.237727934256597 2208 13 0.008243395456389635 2208 14 -0.5174034023571821 2208 15 0.13234760486029984 2208 16 0.11625162477486839 2208 17 0.1213238207554923 2208 18 0.21892105840211684 2208 19 0.4206503605813552 2208 20 0.3904943206980036 2208 21 0.6053643565225784 2208 22 0.5616234921825267 2208 23 0.5132551757408876 2208 24 0.5713550571361523 2208 25 0.5765411415546282 2209 1 -0.037806577258375995 2209 2 -0.02829123308850967 2209 3 0.001521990081400304 2209 4 -0.5430126244855984 2209 5 -0.0868688240857054 2209 6 0.048318265719998976 2209 7 -0.005608678950757451 2209 8 0.055134483656772204 2209 9 0.15502504238802572 2209 10 0.22109157808009172 2209 11 0.16288908519199907 2209 12 0.18538338080058026 2209 13 0.20439935397271825 2209 14 0.17674363650087344 2209 15 -0.43170825395956314 2209 16 -0.4088837967945196 2209 17 -0.03414158103825571 2209 18 -0.50976684323944 2209 19 -0.0032969515091494372 2209 20 0.14854849194418307 2209 21 0.4243165683372965 2209 22 0.3168627931421142 2209 23 0.36038063813803944 2209 24 0.4133163345862272 2209 25 0.5186585424816812 2210 1 -0.24415677282608783 2210 2 -0.16129721515584095 2210 3 -0.08001047227081415 2210 4 -0.06261801784614818 2210 5 -0.2048986111672093 2210 6 -0.6162213307094717 2210 7 -0.24744546733459316 2210 8 -0.36837499540434493 2210 9 -0.014315596646731876 2210 10 0.029397919501896297 2210 11 0.05099584700170112 2210 12 -0.02337276603057603 2210 13 0.0681336789632283 2210 14 0.20257526388025582 2210 15 0.0742024811818709 2210 16 0.19472853661708456 2210 17 -1.1060263399073946 2210 18 -1.4857010589177477 2210 19 -0.30903332054156607 2210 20 -0.2424966213584696 2210 21 -0.44903099886531805 2210 22 -0.22973079274392205 2210 23 -0.22703087083056772 2210 24 0.11996734007962573 2210 25 0.1458936150243062 2211 1 -0.2671395329307771 2211 2 -0.20992662858830258 2211 3 -0.16440229684790353 2211 4 -0.06482589348867407 2211 5 -0.08160761985278953 2211 6 0.04774175915932233 2211 7 -0.16277907980337147 2211 8 -0.05054254021521132 2211 9 -0.0758774724966571 2211 10 0.04283700494215026 2211 11 -0.24452529416807778 2211 12 -0.14101165540316885 2211 13 -0.07367506615471342 2211 14 -0.030388879534143598 2211 15 -0.24611418948039443 2211 16 -0.08259561249185055 2211 17 -0.27505114673782316 2211 18 -0.29007136480987583 2211 19 -0.2155913665119229 2211 20 -0.3861513279169131 2211 21 0.09527047651786802 2211 22 -0.05128129089835198 2211 23 -0.6970791656226254 2211 24 -0.19602789590129532 2211 25 -0.7386416816675094 2212 1 -0.21576509428711788 2212 2 -0.31553981962447253 2212 3 -0.1808747075335937 2212 4 0.019605079231077127 2212 5 -0.08436319990239127 2212 6 -0.10140810715780901 2212 7 -0.12112960448070709 2212 8 -0.2766713970963437 2212 9 -0.1311693033189414 2212 10 -0.30659884589904607 2212 11 -0.08222758725752755 2212 12 -0.1038366462799658 2212 13 -0.164385782382098 2212 14 -0.16957695159628372 2212 15 -0.06894826632384365 2212 16 -0.29893247842915827 2212 17 -0.47519711800531894 2212 18 -0.34594708070212477 2212 19 -0.3192309664314905 2212 20 -0.5922016880041951 2212 21 -0.007316044971929614 2212 22 -0.43606453101363485 2212 23 -0.12201493081741432 2212 24 -0.4042380349463741 2212 25 -0.13310222101645053 2213 1 -0.12486741514377186 2213 2 -0.08628431672427439 2213 3 -0.017854295447115476 2213 4 -0.008317166464010484 2213 5 0.019425726644406893 2213 6 -0.07897489838175176 2213 7 -0.24566013224238503 2213 8 -0.16525376489509114 2213 9 -0.08750979202108766 2213 10 -0.04287187896676414 2213 11 0.060379916822384416 2213 12 0.10310090817191053 2213 13 0.1999321504571938 2213 14 -0.4770223333581214 2213 15 -0.15444703013012884 2213 16 -0.2692262033135151 2213 17 -0.0900811450023582 2213 18 -0.09224299742222541 2213 19 -0.16697889217689266 2213 20 -0.144033715177045 2213 21 0.04610863675799237 2213 22 -0.19602237021521063 2213 23 -0.2888938117653268 2213 24 -0.44370011507900387 2213 25 -0.221307507628957 2214 1 -0.2857607478354005 2214 2 -0.3273320394759054 2214 3 -0.16743742156341312 2214 4 -0.325430252152934 2214 5 -0.18140267358660367 2214 6 -0.41962566682273206 2214 7 -0.3606834675543098 2214 8 -0.3488562464193521 2214 9 -0.4284595211683637 2214 10 -0.23451738944259404 2214 11 -0.2001543956448657 2214 12 -0.45025279927507 2214 13 -0.49849620859778554 2214 14 -0.43216552701380806 2214 15 -0.3223083698484217 2214 16 -0.4389815735769318 2214 17 -0.5399521691090499 2214 18 -0.42021559263510966 2214 19 -0.5813187120718624 2214 20 -0.6351796989098375 2214 21 -0.8004596820300449 2214 22 -1.0689844575190932 2214 23 -1.1419812787442893 2214 24 -0.5048032383076304 2214 25 -0.973312332443669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 9.0 9 25.0 10 45.0 11 91.0 12 298.0 13 689.0 14 1448.0 15 2605.0 16 4147.0 17 5661.0 18 6699.0 19 7406.0 20 7820.0 21 8041.0 22 8629.0 23 9211.0 24 9821.0 25 10970.0 26 12312.0 27 14077.0 28 16791.0 29 20994.0 30 27461.0 31 36748.0 32 50605.0 33 70466.0 34 99263.0 35 143142.0 36 211635.0 37 346673.0 38 773433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.111597520031786 21.222433498989947 12.158310271042492 22.507658709935775 2 14.724289225761527 22.330873902064578 40.3653283110041 22.57950856116979 3 18.585114330880966 28.786664083570514 29.664234251119996 22.963987334428523 4 11.24773726945319 17.785953556079008 38.57685522929353 32.389453945174274 5 11.708417637674366 40.267739049351206 34.09110218158132 13.9327411313931 6 29.29011024969795 39.23943230634985 17.53197786615653 13.938479577795675 7 24.893105186175024 35.554895921450864 22.587334360894094 16.964664531480018 8 25.18391993320775 36.908802564285445 20.60086804754333 17.30640945496348 9 25.709843934742544 16.08748882532908 22.25512068644594 35.94754655348243 10 15.257854054210544 31.31483855006935 32.380521261280705 21.046786134439404 11 31.52571682505172 26.802286872727255 21.958448254966267 19.71354804725476 12 21.009219817994584 29.762279283303865 31.391949228081433 17.836551670620114 13 27.00732376406238 24.52768852610297 27.351752502076327 21.113235207758326 14 20.64514505303327 23.69716007611162 29.629891794196624 26.02780307665849 15 23.42177579487319 29.753443677114667 25.93709806726227 20.88768246074987 16 20.198111065727844 31.808140988971868 27.379112873077105 20.614635072223187 17 18.305392053539876 30.732419120245552 29.952118640068708 21.010070186145864 18 18.519536097960742 29.264848760993132 32.402149104478625 19.813466036567494 19 19.32515836017456 28.22525971278359 33.62274493066548 18.826836996376368 20 21.450136167205148 27.070271086516772 32.559324753278865 18.92026799299921 21 21.693580269440137 28.024857200083474 31.412258928468432 18.869303602007957 22 19.944671687392937 27.682717924705884 33.047131311934336 19.325479075966843 23 19.536129906696413 28.110150140387947 32.800287329954934 19.553432622960703 24 19.329745555825877 27.769376934757258 32.86156366137868 20.039313848038187 25 20.06298274581072 27.64639477262086 31.774669421855933 20.515953059712487 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1385.0 1 1385.0 2 1648.0 3 1911.0 4 1911.0 5 1911.0 6 4658.0 7 7405.0 8 7405.0 9 7405.0 10 9980.0 11 12555.0 12 12555.0 13 12555.0 14 15658.5 15 18762.0 16 18762.0 17 18762.0 18 30001.5 19 41241.0 20 41241.0 21 41241.0 22 61397.5 23 81554.0 24 81554.0 25 81554.0 26 106606.5 27 131659.0 28 131659.0 29 131659.0 30 156090.5 31 180522.0 32 180522.0 33 180522.0 34 195684.5 35 210847.0 36 210847.0 37 210847.0 38 215472.0 39 220097.0 40 220097.0 41 220097.0 42 219053.5 43 218010.0 44 218010.0 45 218010.0 46 226794.5 47 235579.0 48 235579.0 49 235579.0 50 218551.0 51 201523.0 52 201523.0 53 201523.0 54 174721.5 55 147920.0 56 147920.0 57 147920.0 58 122895.0 59 97870.0 60 97870.0 61 97870.0 62 77400.5 63 56931.0 64 56931.0 65 56931.0 66 41387.5 67 25844.0 68 25844.0 69 25844.0 70 18084.0 71 10324.0 72 10324.0 73 10324.0 74 6956.0 75 3588.0 76 3588.0 77 3588.0 78 2395.5 79 1203.0 80 1203.0 81 1203.0 82 777.0 83 351.0 84 351.0 85 351.0 86 227.5 87 104.0 88 104.0 89 104.0 90 65.0 91 26.0 92 26.0 93 26.0 94 15.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09799624164226811 2 0.0 3 0.1323919262422295 4 0.01195459769632805 5 0.02275568157985252 6 0.014261625321935218 7 0.022808114025889045 8 0.02139243798290283 9 5.2432446036526536E-5 10 0.3880525331163329 11 0.06328596236608754 12 0.0010486489207305308 13 0.0 14 8.913515826209511E-4 15 0.0013108111509131633 16 1.5729733810957962E-4 17 1.0486489207305307E-4 18 1.5729733810957962E-4 19 6.81621798474845E-4 20 1.0486489207305307E-4 21 1.0486489207305307E-4 22 0.00288378453200896 23 5.2432446036526536E-5 24 0.0 25 0.0014156760429862166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1907216.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.81340473702612 #Duplication Level Percentage of deduplicated Percentage of total 1 90.39303475801245 68.53003729517268 2 7.473268455723195 11.331478522843856 3 1.1192004094357628 2.5455118086719652 4 0.328087996002332 0.9949387212113843 5 0.1724317374649891 0.6536318550970923 6 0.09962271476505215 0.45316423172905246 7 0.06579091206744248 0.34914831312109784 8 0.05343164378857668 0.3240667869046377 9 0.03677220719738039 0.2509043604595906 >10 0.21816763314355853 3.1625416210767505 >50 0.02220925022962337 1.1560532977981544 >100 0.014164551274754324 2.2063825928161007 >500 0.0018741588029280304 0.969081598876418 >1k 0.0015270923579413524 2.541714479724789 >5k 1.388265779946684E-4 0.7302712651429822 >10k+ 2.776531559893368E-4 3.8010732493534345 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 26647 1.3971673895353227 No Hit GGTATCAACGCAGAGTACTTTTTTT 19010 0.9967407991543695 No Hit TATCAACGCAGAGTACTTTTTTTTT 16456 0.8628283319770808 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10117 0.530459056551539 No Hit GTACATGGGAAGCAGTGGTATCAAC 7285 0.3819703693760958 No Hit CCCATGTACTCTGCGTTGATACCAC 6592 0.34563468427278293 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4766 0.24989303781008548 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4349 0.2280287078128539 No Hit GAGTACATGGGAAGCAGTGGTATCA 3633 0.19048707645070093 No Hit GTGGTATCAACGCAGAGTACTTTTT 2745 0.14392706437026537 No Hit CATGTACTCTGCGTTGATACCACTG 2678 0.14041409048581807 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2622 0.13747787350777257 No Hit GGTATCAACGCAGAGTACATGGGAA 2503 0.13123841242942594 No Hit GTGGTATCAACGCAGAGTACATGGG 2331 0.12222003171114336 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2300 0.12059462588401104 No Hit ATTCCATTCCATTCCATTCCATTCC 2269 0.11896922005687872 No Hit GTATCAACGCAGAGTACATGGGAAG 2212 0.11598057063279671 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2114 0.11084219092121711 No Hit GCGTTGATACCACTGCTTCCCATGT 2032 0.10654273034622191 No Hit GAATGGAATGGAATGGAATGGAATG 1999 0.10481245962701655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.2432446036526536E-5 0.0 3 0.0 0.0 0.0 5.2432446036526536E-5 0.0 4 0.0 0.0 0.0 1.0486489207305307E-4 0.0 5 0.0 0.0 0.0 1.0486489207305307E-4 0.0 6 0.0 0.0 0.0 1.0486489207305307E-4 0.0 7 0.0 0.0 0.0 1.0486489207305307E-4 0.0 8 0.0 0.0 0.0 1.0486489207305307E-4 0.0 9 0.0 0.0 0.0 1.0486489207305307E-4 0.0 10 0.0 0.0 0.0 1.0486489207305307E-4 0.0 11 0.0 0.0 0.0 1.0486489207305307E-4 0.0 12 0.0 0.0 0.0 1.0486489207305307E-4 0.0 13 0.0 0.0 0.0 1.0486489207305307E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCCCG 55 1.9918408E-4 13.792382 17 CCCGTAC 75 1.507281E-5 12.643349 16 GCGCCTT 60 4.158725E-4 12.642686 13 ATCCGAA 55 0.0031127448 12.068018 12 CAGCGTA 65 7.8865956E-4 11.716557 10 CGCAGCG 75 2.0337521E-4 11.424243 8 GAGTGCC 75 2.0347469E-4 11.423642 9 CCCCGTA 85 5.4278447E-5 11.155312 15 CCCCCGG 70 0.0015151051 10.836872 18 CCGTACA 90 9.6987504E-5 10.535848 17 CTAGGAC 155 3.6543497E-9 10.420939 3 GGTATCA 6085 0.0 10.253371 1 TAGACAG 260 0.0 10.252527 5 CGCCCTA 85 6.707043E-4 10.040307 16 TGTCCCG 95 1.6636334E-4 9.9884 11 GTTAGCC 105 4.0986703E-5 9.953896 3 ACGCAGC 100 2.6973922E-4 9.520204 7 GTATCGC 90 0.0010923211 9.520204 6 CTCTAGG 180 4.100002E-9 9.510941 1 CCTAGTA 240 1.8189894E-12 9.501445 2 >>END_MODULE