FastQCFastQC Report
Wed 25 May 2016
SRR1294664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440031
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT191831.332124100106178No Hit
GGTATCAACGCAGAGTACTTTTTTT119760.8316487631169052No Hit
TATCAACGCAGAGTACTTTTTTTTT110600.7680390213821785No Hit
GTACTGGTTCACTATCGGTCAGTCA70940.4926282836966704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48220.3348539024507111No Hit
GGATACCACGTGTCCCGCCCTACTC44960.31221550091629974No Hit
ATACAGGGTGACAGCCCCGTACACA43820.3042990046742049No Hit
GTTAATGATAGTGTGTCGAAACACA40410.2806189588974126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31810.2208980223342414No Hit
ACCCTGTATCGCGCGCCTTTCCAGA29550.20520391574903596No Hit
ACTTAGATGTTTCAGTTCCCCCGGT28250.1961763323150682No Hit
GGTATCAACGCAGAGTACATGGGGG28150.19548190282014763No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27700.19235697009300495No Hit
GTATTTAGCCTTGGAGGATGGTCCC26480.18388493025497368No Hit
GTTCACTATCGGTCAGTCAGGAGTA26340.18291272896208485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25580.17763506480068833No Hit
GTACATGGGGGTTAAGCGACTAAGC23010.159788226781229No Hit
AGCGTACACGGTGGATGCCCTGGCA22400.15555220686221338No Hit
CCCATATTCAGACAGGATACCACGT22140.15374669017541984No Hit
TCATTAACCTATGGATTCAGTTAAT21940.15235783118557863No Hit
GTATCAACGCAGAGTACATGGGGGT21670.15048287154929305No Hit
GTGGATGCCCTGGCAGTCAGAGGCG21490.14923289845843596No Hit
ATCATTAACTGAATCCATAGGTTAA21380.14846902601402331No Hit
CTATCGGTCAGTCAGGAGTATTTAG20980.1456913080343409No Hit
GCCTTGGAGGATGGTCCCCCCATAT20940.1454135362363727No Hit
TATTCAGACAGGATACCACGTGTCC20750.14409412019602355No Hit
GCCCAGAGCCTGAATCAGTGTGTGT20390.1415941740143094No Hit
GTCAGGAGTATTTAGCCTTGGAGGA19840.13777481179224613No Hit
GTACTTAGATGTTTCAGTTCCCCCG19790.13742759704478585No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG19540.13569152330748435No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19220.13346934892373843No Hit
GGTTAATGAGGCGAACCGGGGGAAC18360.12749725526742134No Hit
GGTATCAACGCAGAGTACATGGGGA17040.11833078593446945No Hit
CCTATGGATTCAGTTAATGATAGTG16870.11715025579310445No Hit
CGGTACTGGTTCACTATCGGTCAGT15960.11083094738932704No Hit
ATCAACGCAGAGTACTTTTTTTTTT15790.10965041724796203No Hit
TCTAAGTACCCCGAGGAAAAGAAAT15470.10742824286421612No Hit
CACACACACTGATTCAGGCTCTGGG14950.10381720949062903No Hit
GGTTCACTATCGGTCAGTCAGGAGT14550.10103949151094664No Hit
GTGGTATCAACGCAGAGTACATGGG14520.10083116266247046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC400.00528898214.2458823
GGTATCA35400.013.9824571
GTACATA3850.013.5722231
ATCGCCG2200.013.38482219
AATCGCC2350.013.34723818
CTAAGAC500.001502722413.2961573
GCTCTAG655.448945E-513.1546161
TTACACC802.0048228E-613.0591784
GTATATA701.0901956E-412.2150011
AAATCGC2650.012.19320817
GTTATAT802.8642968E-511.8756951
TGGTTCG802.8734808E-511.8719815
TACATGA3200.011.5747792
ATACCGT752.078514E-411.3971016
TACATAA3100.011.3354332
TCGGTCG600.005859078411.0874515
GTATAGA1201.2736564E-711.08398151
TCAGTGC600.005886430411.0805168
GAACCCG700.001486671210.861176517
GTACAAA3350.010.776751
Produced by FastQC (version 0.11.5)