FastQCFastQC Report
Wed 25 May 2016
SRR1294664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440031
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT534843.714086710633313No Hit
GGTATCAACGCAGAGTACTTTTTTT365862.5406397501164903No Hit
TATCAACGCAGAGTACTTTTTTTTT341062.3684212353761827No Hit
ACGCAGAGTACTTTTTTTTTTTTTT189491.3158744499250363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT111630.7751916451798607No Hit
GAGTACTTTTTTTTTTTTTTTTTTT101540.7051237091423727No Hit
GCAGAGTACTTTTTTTTTTTTTTTT70560.4899894516159722No Hit
GTACTGGTTCACTATCGGTCAGTCA66080.4588790102435295No Hit
ATCAACGCAGAGTACTTTTTTTTTT54530.3786724035802007No Hit
GGATACCACGTGTCCCGCCCTACTC46490.32284027218858485No Hit
ATACAGGGTGACAGCCCCGTACACA45390.3152015477444583No Hit
GTTAATGATAGTGTGTCGAAACACA39540.2745774222916034No Hit
GTGGTATCAACGCAGAGTACTTTTT38250.2656192818071278No Hit
ACCCTGTATCGCGCGCCTTTCCAGA31880.22138412298068583No Hit
GGTATCAACGCAGAGTACATGGGGG30430.21131489530433722No Hit
ACTTAGATGTTTCAGTTCCCCCGGT29640.2058289022944645No Hit
AGCGTACACGGTGGATGCCCTGGCA26900.18680153413364017No Hit
CCCATATTCAGACAGGATACCACGT25590.17770450775018037No Hit
CTATCGGTCAGTCAGGAGTATTTAG24600.1708296557504665No Hit
GTCAGGAGTATTTAGCCTTGGAGGA23040.1599965556297052No Hit
GTTCACTATCGGTCAGTCAGGAGTA22990.15964934088224492No Hit
GTATTTAGCCTTGGAGGATGGTCCC22410.15562164981170543No Hit
CAACGCAGAGTACTTTTTTTTTTTT21750.15103841514522953No Hit
GTGGATGCCCTGGCAGTCAGAGGCG21700.1506912003977692No Hit
GCCTTGGAGGATGGTCCCCCCATAT21430.14881624076148361No Hit
TCATTAACCTATGGATTCAGTTAAT21230.1474273817716424No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21180.1470801670241821No Hit
GTATCAACGCAGAGTACATGGGGGT21160.146941281125198No Hit
ATCATTAACTGAATCCATAGGTTAA21110.1465940663777377No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG20830.14464966379196004No Hit
GTACATGGGGGTTAAGCGACTAAGC20310.14103863041837295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20120.13971921437802381No Hit
GTACTTAGATGTTTCAGTTCCCCCG19990.13881645603462703No Hit
TATTCAGACAGGATACCACGTGTCC19630.13631650985291288No Hit
CCTATGGATTCAGTTAATGATAGTG18470.12826112771183398No Hit
CACACACACTGATTCAGGCTCTGGG18360.12749725526742134No Hit
CGGTACTGGTTCACTATCGGTCAGT18240.12666393987351662No Hit
GGTATCAACGCAGAGTACATGGGGA18130.12590006742910395No Hit
GGTTAATGAGGCGAACCGGGGGAAC17790.12353900714637393No Hit
AACGCAGAGTACTTTTTTTTTTTTT17740.12319179239891365No Hit
TCACGGTACTGGTTCACTATCGGTC16830.11687248399513622No Hit
GCCTGAATCAGTGTGTGTGTTAGTG15990.11103927623780321No Hit
GTGGTATCAACGCAGAGTACATGGG15930.11062261854085086No Hit
TCTAAGTACCCCGAGGAAAAGAAAT15710.10909487365202554No Hit
TCAACGCAGAGTACTTTTTTTTTTT15600.10833100120761288No Hit
CATCTAAGTACCCCGAGGAAAAGAA15380.10680325631878759No Hit
GGGTACTTAGATGTTTCAGTTCCCC15090.10478941078351785No Hit
CCCATGTACTCTGCGTTGATACCAC15070.10465052488453375No Hit
GGTTCACTATCGGTCAGTCAGGAGT15000.10416442423808932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64400.015.2241241
CAGCGCG456.664696E-414.8060229
GCGTACT851.7560524E-814.504106512
TAAGTGT400.005208780514.2787286
CTCTCGG400.005340248314.22518312
ATCTATA500.001498335413.3013142
GTACATA2150.012.3845171
CGACGGG701.1086854E-412.19301315
TCTACGG550.003065981412.0921062
CGTACTT951.0616986E-611.97910113
CAGCGTA1302.5356712E-911.71465510
AGGACTA752.0351766E-411.4229825
GTATTAG1001.9059025E-611.41144851
ATACACT1104.83893E-711.2499074
CGTGCGA855.219464E-511.19783110
CGCAGTA600.005870973711.0844292
GACGGGA600.0059520211.06403116
ACAGGGT9450.011.0592943
CGCAGAA2500.011.021092
CTGCTAG700.001478848510.8680461
Produced by FastQC (version 0.11.5)