FastQCFastQC Report
Wed 25 May 2016
SRR1294663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307882
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC350160.8128356347736544No Hit
CCCATGTACTCTGCGTTGATACCAC329350.7645288334267281No Hit
GAGTACATGGGAAGCAGTGGTATCA160530.3726425189919315No Hit
CATGTACTCTGCGTTGATACCACTG127430.29580661680148157No Hit
GTATCAACGCAGAGTACTTTTTTTT116460.27034166674017535No Hit
GCGTTGATACCACTGCTTCCCATGT106410.24701233692102056No Hit
GTATCAACGCAGAGTACATGGGAAG95930.22268483677129502No Hit
ATTCCATTCCATTCCATTCCATTCC82000.19034876071350143No Hit
ACGCAGAGTACATGGGAAGCAGTGG81860.19002377502447837No Hit
GGTATCAACGCAGAGTACTTTTTTT75380.17498158027541144No Hit
TATCAACGCAGAGTACATGGGAAGC73550.17073355305461013No Hit
ACTCTGCGTTGATACCACTGCTTCC70950.16469810454418202No Hit
GGTATCAACGCAGAGTACATGGGAA67900.15761805917617985No Hit
TATCAACGCAGAGTACTTTTTTTTT67220.1560395572580679No Hit
GAATGGAATGGAATGGAATGGAATG62870.14594178763485166No Hit
GTACTGGTTCACTATCGGTCAGTCA62850.14589536110784837No Hit
GCTTCCCATGTACTCTGCGTTGATA58950.1368421883422062No Hit
GTGGTATCAACGCAGAGTACATGGG48490.11256111471948395No Hit
GGATACCACGTGTCCCGCCCTACTC46460.10784882222864972No Hit
CAGTGGTATCAACGCAGAGTACATG43330.10058307075263435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGC350.002175973716.280188
GACCGTT752.0817794E-411.3962577
GAACGAT1002.3882638E-510.4550716
ATCGCCG1752.3646862E-1010.31173919
AAATCGC1856.002665E-1110.275372517
GCGACTA8800.010.26065716
CCGGTTG750.002638816410.13813114
GTACTGG19050.010.0238251
AATCGCC1909.822543E-1110.00578218
TAGCACG951.650624E-49.9968344
ACCGGGG4100.09.96813215
TCGGAAC1054.0869585E-59.9572113
CCGTACA8900.09.93189717
GGACCGT1252.5833833E-69.8768716
CCCCGTA8600.09.725533515
TAGACAG4650.09.599115
CGACTAA9350.09.55554717
GAAATCG2201.6370905E-119.50460916
CCCGTAC9000.09.50460916
TGTCCCG9900.09.49655111
Produced by FastQC (version 0.11.5)