##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294663_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4307882 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.215101527850578 31.0 30.0 34.0 26.0 34.0 2 30.191100174053048 31.0 30.0 34.0 25.0 34.0 3 30.22500941297835 31.0 30.0 34.0 25.0 34.0 4 33.80389667126444 35.0 35.0 37.0 28.0 37.0 5 34.21405832378881 37.0 35.0 37.0 30.0 37.0 6 34.28781661150421 37.0 35.0 37.0 30.0 37.0 7 34.16720165501283 37.0 35.0 37.0 30.0 37.0 8 34.07254191270791 37.0 35.0 37.0 29.0 37.0 9 35.1390934106366 39.0 35.0 39.0 27.0 39.0 10 35.24693109049876 39.0 35.0 39.0 28.0 39.0 11 35.524800354327255 39.0 35.0 39.0 29.0 39.0 12 35.30227499267622 39.0 35.0 39.0 27.0 39.0 13 35.16176882282291 39.0 35.0 39.0 27.0 39.0 14 36.071705770956584 39.0 36.0 41.0 27.0 41.0 15 36.05958426902129 39.0 35.0 41.0 27.0 41.0 16 36.16030754788548 39.0 36.0 41.0 27.0 41.0 17 36.155549293132914 39.0 36.0 41.0 27.0 41.0 18 36.0307046478989 39.0 35.0 41.0 27.0 41.0 19 36.302429593011134 39.0 36.0 41.0 27.0 41.0 20 36.44167505052367 39.0 36.0 41.0 27.0 41.0 21 36.47778978161426 39.0 36.0 41.0 27.0 41.0 22 36.4809349466861 39.0 36.0 41.0 27.0 41.0 23 36.47069139776809 39.0 36.0 41.0 27.0 41.0 24 36.53446566085143 39.0 36.0 41.0 28.0 41.0 25 35.34364288529723 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0706826280040396 1101 2 1.0466659736573298 1101 3 1.264527499591793 1101 4 1.1606886158134273 1101 5 0.7738311408433134 1101 6 0.792731134993204 1101 7 0.7612522614197488 1101 8 0.898047618806693 1101 9 1.2293420330054161 1101 10 1.1822134851240662 1101 11 0.9403799270242317 1101 12 1.1290325347040167 1101 13 1.158036160720819 1101 14 1.3844686266950248 1101 15 1.4684649620310353 1101 16 1.347008090469707 1101 17 1.3853098037739997 1101 18 1.3767627227287988 1101 19 1.230660585535297 1101 20 1.2346252155680304 1101 21 1.089952403741222 1101 22 1.0339902754808037 1101 23 1.0063883202218733 1101 24 1.0762057843864739 1101 25 1.0973498500918524 1102 1 0.9471948821213978 1102 2 0.9319877741103326 1102 3 1.2141291667963117 1102 4 1.0050286295714415 1102 5 0.8051917537414184 1102 6 0.6663921952065976 1102 7 0.592945376324181 1102 8 0.7312946081803062 1102 9 1.08413018429858 1102 10 1.0031019408531279 1102 11 0.7969078601448203 1102 12 1.0031200730213143 1102 13 0.9995986235051433 1102 14 1.2168727832437085 1102 15 1.2929808864866956 1102 16 1.2259121701930056 1102 17 1.29033776979918 1102 18 1.383241284557542 1102 19 1.0757161770195012 1102 20 0.23473824320976178 1102 21 0.5846751116122206 1102 22 0.6734427977511714 1102 23 0.8396364472160371 1102 24 0.8361232219350683 1102 25 0.926586021414046 1103 1 0.9135191589214529 1103 2 0.8980142261010933 1103 3 1.1909864021366303 1103 4 0.999571107482943 1103 5 0.7995082813862453 1103 6 0.7074055421671162 1103 7 0.5653099413905807 1103 8 0.8123325583933507 1103 9 0.9356496945153339 1103 10 0.963609095580324 1103 11 0.877124096836944 1103 12 1.1014023737248522 1103 13 0.9110418859116578 1103 14 0.9639456001666957 1103 15 1.14158667944983 1103 16 1.1546854075451307 1103 17 1.137147068512732 1103 18 1.311013803846059 1103 19 1.0471550945673016 1103 20 0.21892244896936575 1103 21 0.5466831374702394 1103 22 -0.037230833189362045 1103 23 0.5716132664544631 1103 24 0.7798793042827299 1103 25 0.7531837584057186 1104 1 0.45225803956023114 1104 2 0.43646717867555296 1104 3 0.6914851617243372 1104 4 0.5696960082682452 1104 5 0.4571502018352618 1104 6 0.2863835256490077 1104 7 0.3446309128167542 1104 8 0.4438622112425037 1104 9 0.6797296748187023 1104 10 0.7108044516010565 1104 11 0.4124225250384299 1104 12 0.49069831973011446 1104 13 0.4750462230111836 1104 14 0.7547581022241445 1104 15 0.7691413435463659 1104 16 0.6938630960347609 1104 17 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0.36175682804638853 2207 7 0.31690452675437797 2207 8 0.30402117279857777 2207 9 -1.256997607673334 2207 10 -0.21163244691143746 2207 11 0.18439228998643387 2207 12 0.5098732618688473 2207 13 0.59491346699388 2207 14 0.813389541886643 2207 15 0.8465439341750525 2207 16 0.8642745740474851 2207 17 0.8157820013315273 2207 18 0.861328847788478 2207 19 -0.3315855571044821 2207 20 0.3290993629999548 2207 21 -0.2270239412036048 2207 22 0.26267004988435616 2207 23 -0.4949306337173667 2207 24 -1.6965284905681202 2207 25 -0.2009042426365042 2208 1 0.5285311901195939 2208 2 0.23984459312178075 2208 3 0.6929699940715786 2208 4 0.5586605393891233 2208 5 0.41012356653946824 2208 6 0.3729460126284465 2208 7 0.4138983499534774 2208 8 0.5695780708947353 2208 9 -0.227078310518408 2208 10 0.16254824122696476 2208 11 0.31907529442517557 2208 12 0.24900495006072987 2208 13 0.5890370665179177 2208 14 0.845465956400794 2208 15 0.7167609243884314 2208 16 0.538828945270545 2208 17 0.5326874006844307 2208 18 0.7179638951172151 2208 19 -1.3562409531149626 2208 20 -0.10675741744285006 2208 21 -0.18716636192129243 2208 22 0.23081423540229906 2208 23 0.40085841248831144 2208 24 0.4494682037738187 2208 25 0.5778627535932443 2209 1 0.07373864402830321 2209 2 -1.4181379990936378 2209 3 -0.27044662138938236 2209 4 0.039608040920164456 2209 5 0.036018499103917634 2209 6 0.11001327568251895 2209 7 0.06062809096549415 2209 8 0.25597382603418595 2209 9 -2.0636856663050835 2209 10 -0.4764106877959762 2209 11 -0.133403502924196 2209 12 0.21744075072913205 2209 13 0.3340415985819263 2209 14 0.4336712970223715 2209 15 0.6488495383741792 2209 16 0.5920791452703753 2209 17 0.3149463659926184 2209 18 0.5777558008806594 2209 19 0.2606830145205663 2209 20 0.30465341721218664 2209 21 0.3404561141197746 2209 22 0.32047611664446407 2209 23 0.19685973439590043 2209 24 0.28415480690436823 2209 25 0.29209016879905647 2210 1 0.11333616061684282 2210 2 0.140221505817685 2210 3 0.06335717754707204 2210 4 0.16811728556991312 2210 5 0.08151104940777998 2210 6 0.01264032618291111 2210 7 0.011221296374110068 2210 8 0.2043978308615948 2210 9 0.3648760790250023 2210 10 -1.5469329104099288 2210 11 -0.48758603094191955 2210 12 -2.235059254872212 2210 13 -0.7708795019297838 2210 14 -0.5770389216017833 2210 15 -0.792660065559879 2210 16 -0.1731887059331214 2210 17 0.07706103447307555 2210 18 0.3519722418758633 2210 19 0.19311088409441624 2210 20 0.21858463472809575 2210 21 0.15961704941067012 2210 22 0.20051517822197695 2210 23 0.11343683322623122 2210 24 0.2041277618173183 2210 25 0.04007015065482733 2211 1 -0.3631640806660634 2211 2 -0.09174639592317035 2211 3 -1.5236419713273008 2211 4 -0.6974682218881298 2211 5 -0.5035320645265742 2211 6 -0.42357961436069047 2211 7 -0.4501911513485055 2211 8 -0.056887931301538686 2211 9 0.09244535692418765 2211 10 0.13248666839496082 2211 11 -0.20032470952021697 2211 12 -0.20505301273419008 2211 13 -0.21292572675649524 2211 14 -0.7767190859464534 2211 15 -0.2948375526888185 2211 16 -0.15062283377414332 2211 17 -0.16783441899782758 2211 18 -0.05497627953230477 2211 19 -0.0696996842756974 2211 20 -0.037821202984289926 2211 21 -0.1258117596171786 2211 22 -0.19825881031168535 2211 23 -0.6088166019781411 2211 24 -0.3657890935643522 2211 25 -0.2800145988489575 2212 1 -0.36552804874298417 2212 2 -0.41643526157568544 2212 3 -2.4856199691251106 2212 4 -1.4719531928342136 2212 5 -0.6717741185663471 2212 6 -0.4801985073214112 2212 7 -0.3377938254595563 2212 8 -0.3285053021704556 2212 9 -0.9771995708129779 2212 10 -0.27714554890667387 2212 11 -0.37147981768116267 2212 12 -0.05813911721379128 2212 13 -0.5126390487419741 2212 14 -0.09286830103308574 2212 15 0.027112725446997388 2212 16 -1.0668562931384713 2212 17 -1.3241899051026707 2212 18 -0.2818553026027999 2212 19 -0.20000858691757628 2212 20 -0.20752673114417775 2212 21 -0.09050064614068987 2212 22 -0.40849269365535434 2212 23 -0.07520303082280577 2212 24 -0.20956308216342023 2212 25 -0.03003418776788891 2213 1 -3.24820923097084 2213 2 -1.503453759234695 2213 3 -2.5682343349558288 2213 4 -1.1073235060494326 2213 5 -0.8213520760761099 2213 6 -0.6173090111083894 2213 7 -0.3545881879469093 2213 8 -0.25716900221623007 2213 9 -0.4547425441121149 2213 10 -0.29395835841913254 2213 11 -0.5985006765593894 2213 12 -0.2544031875231596 2213 13 -0.7192999260112458 2213 14 -0.43455523186284495 2213 15 -0.37038218126576083 2213 16 -1.7094974694938116 2213 17 -1.1617659481928868 2213 18 -0.44640531096121094 2213 19 -0.3524143461551503 2213 20 -0.21926629070046744 2213 21 -0.021322838955100565 2213 22 -0.07997973760990362 2213 23 -0.08868271734042565 2213 24 -0.06382420501984853 2213 25 -0.07128109979949926 2214 1 -1.2614115098961491 2214 2 -1.034821098395497 2214 3 -6.553571939226515 2214 4 -2.360999170493386 2214 5 -1.4557060578522893 2214 6 -0.8777781447412636 2214 7 -0.7161646013049818 2214 8 -0.5760022788844594 2214 9 -0.5354446447626913 2214 10 -0.49043968257173987 2214 11 -1.1440229627705918 2214 12 -0.7798079379371998 2214 13 -5.537049198823933 2214 14 -4.204006474190688 2214 15 -2.562059168281216 2214 16 -3.245742525541516 2214 17 -3.732835738143848 2214 18 -1.4452159447352315 2214 19 -1.0135961380418976 2214 20 -0.7652990302209162 2214 21 -0.7868571116382554 2214 22 -0.6646858086457783 2214 23 -0.6096424844054553 2214 24 -0.39788020759020526 2214 25 -0.3412026001596544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1554.0 3 195.0 4 215.0 5 488.0 6 49.0 7 90.0 8 193.0 9 419.0 10 981.0 11 2295.0 12 4904.0 13 8696.0 14 14293.0 15 20437.0 16 25828.0 17 29042.0 18 30213.0 19 30050.0 20 30180.0 21 29964.0 22 30321.0 23 31338.0 24 32926.0 25 35787.0 26 39497.0 27 45373.0 28 53640.0 29 65398.0 30 81846.0 31 104170.0 32 138387.0 33 183770.0 34 251013.0 35 346377.0 36 490669.0 37 742271.0 38 1405013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.521478892875066 21.55313028607543 12.54321802330985 22.382172797739653 2 14.239303991573951 22.80700328286142 40.610470551211826 22.343222174352807 3 18.077548201112688 29.625098069818744 29.81624421742259 22.48110951164598 4 11.591213331983214 18.435313702739283 38.86621660663906 31.107256358638445 5 12.320749147624415 39.670619918172875 34.76085293992648 13.247777994276236 6 29.182943007005917 39.27654653010529 18.526561570500665 13.01394889238812 7 24.211892822998514 34.58921800628627 24.590384023978153 16.608505146737055 8 23.531884728516665 37.88874581222023 22.466567442067888 16.112802017195214 9 24.89000981845726 17.289845643259934 22.432399097175782 35.38774544110703 10 15.13330728710577 30.860993513374396 33.16397091308442 20.841728286435412 11 30.886330501465252 28.112583189076606 22.684148266724392 18.316938042733746 12 20.23579051816265 29.41230985562836 32.273403282011884 18.078496344197113 13 26.024311584062175 25.458109726143373 26.987437737422347 21.530140952372104 14 19.711093009227547 24.7785442044048 28.87497413048826 26.6353886558794 15 23.930116093785568 30.204917842393048 24.39198260683741 21.472983456983975 16 20.62503222737499 32.19567022023317 26.882996163374557 20.296301389017284 17 19.918991232257266 31.198107968177187 29.20127296837578 19.68162783118977 18 20.369968306894517 30.22544551431211 29.981505768870463 19.423080409922914 19 20.329378184217916 29.665778633832446 30.41829528822275 19.586547893726884 20 22.30816864741923 28.351326027241985 28.83387460844464 20.506630716894147 21 21.9728635680766 29.98437295313094 28.49887442525078 19.543889053541683 22 20.707729072486213 29.474495728576333 28.915301354990635 20.902473843946815 23 19.99902907411997 30.289821375188758 29.282427704928654 20.428721845762617 24 20.48128290074272 29.434861000967715 28.92728770353687 21.156568394752696 25 21.481925749010404 28.331792893318003 27.822437246329716 22.363844111341873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5806.0 1 5806.0 2 6211.5 3 6617.0 4 6617.0 5 6617.0 6 9796.0 7 12975.0 8 12975.0 9 12975.0 10 18470.0 11 23965.0 12 23965.0 13 23965.0 14 36740.5 15 49516.0 16 49516.0 17 49516.0 18 74967.5 19 100419.0 20 100419.0 21 100419.0 22 139840.5 23 179262.0 24 179262.0 25 179262.0 26 229710.0 27 280158.0 28 280158.0 29 280158.0 30 329435.5 31 378713.0 32 378713.0 33 378713.0 34 421521.5 35 464330.0 36 464330.0 37 464330.0 38 488370.0 39 512410.0 40 512410.0 41 512410.0 42 506721.5 43 501033.0 44 501033.0 45 501033.0 46 547924.5 47 594816.0 48 594816.0 49 594816.0 50 527311.5 51 459807.0 52 459807.0 53 459807.0 54 378473.5 55 297140.0 56 297140.0 57 297140.0 58 244328.5 59 191517.0 60 191517.0 61 191517.0 62 160870.0 63 130223.0 64 130223.0 65 130223.0 66 98155.5 67 66088.0 68 66088.0 69 66088.0 70 49518.0 71 32948.0 72 32948.0 73 32948.0 74 23014.5 75 13081.0 76 13081.0 77 13081.0 78 8875.5 79 4670.0 80 4670.0 81 4670.0 82 3111.5 83 1553.0 84 1553.0 85 1553.0 86 1037.0 87 521.0 88 521.0 89 521.0 90 370.5 91 220.0 92 220.0 93 220.0 94 145.5 95 71.0 96 71.0 97 71.0 98 47.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09568507215378695 2 0.05076740727810094 3 0.05283338772974747 4 0.048422867664434634 5 0.04584619541575187 6 0.06520605717612507 7 0.06105088300933034 8 0.057986732227113 9 0.06325614304198676 10 0.06304722367047194 11 0.05754568022058172 12 0.058845622976673916 13 0.05865991686866075 14 0.060841963637815515 15 0.06365076852151474 16 0.05886883624017557 17 0.061956200285894554 18 0.1392795810098791 19 0.06641314687821069 20 0.06072589732030729 21 0.0586367036051591 22 0.06406860726454439 23 0.06297758387996699 24 0.06703990499275514 25 0.06580960202716787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4307882.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.61439256876412 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74722668109384 48.338297043180425 2 13.171124185961801 15.96719383959665 3 3.775396993389135 6.865301863806623 4 1.3957789197008625 3.3841716551181427 5 0.6379387506575261 1.933413493359111 6 0.3554998700219365 1.2929045207792569 7 0.2063295701545565 0.8754579094724851 8 0.13294694494286854 0.6446798649267923 9 0.08564822330912349 0.4672363527428746 >10 0.41973712472036995 4.601176186490468 >50 0.03496847867632991 1.4824833235160235 >100 0.030210952599603578 3.8840372690167038 >500 0.0030717356561015823 1.296622687796079 >1k 0.0034605627483073223 4.277358071115735 >5k 4.277100025997719E-4 1.8842096269321862 >10k+ 2.3329636505442107E-4 2.8054562921502746 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 35016 0.8128356347736544 No Hit CCCATGTACTCTGCGTTGATACCAC 32935 0.7645288334267281 No Hit GAGTACATGGGAAGCAGTGGTATCA 16053 0.3726425189919315 No Hit CATGTACTCTGCGTTGATACCACTG 12743 0.29580661680148157 No Hit GTATCAACGCAGAGTACTTTTTTTT 11646 0.27034166674017535 No Hit GCGTTGATACCACTGCTTCCCATGT 10641 0.24701233692102056 No Hit GTATCAACGCAGAGTACATGGGAAG 9593 0.22268483677129502 No Hit ATTCCATTCCATTCCATTCCATTCC 8200 0.19034876071350143 No Hit ACGCAGAGTACATGGGAAGCAGTGG 8186 0.19002377502447837 No Hit GGTATCAACGCAGAGTACTTTTTTT 7538 0.17498158027541144 No Hit TATCAACGCAGAGTACATGGGAAGC 7355 0.17073355305461013 No Hit ACTCTGCGTTGATACCACTGCTTCC 7095 0.16469810454418202 No Hit GGTATCAACGCAGAGTACATGGGAA 6790 0.15761805917617985 No Hit TATCAACGCAGAGTACTTTTTTTTT 6722 0.1560395572580679 No Hit GAATGGAATGGAATGGAATGGAATG 6287 0.14594178763485166 No Hit GTACTGGTTCACTATCGGTCAGTCA 6285 0.14589536110784837 No Hit GCTTCCCATGTACTCTGCGTTGATA 5895 0.1368421883422062 No Hit GTGGTATCAACGCAGAGTACATGGG 4849 0.11256111471948395 No Hit GGATACCACGTGTCCCGCCCTACTC 4646 0.10784882222864972 No Hit CAGTGGTATCAACGCAGAGTACATG 4333 0.10058307075263435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.6426527003293034E-5 0.0 5 0.0 2.3213263501646517E-5 0.0 4.6426527003293034E-5 0.0 6 0.0 2.3213263501646517E-5 0.0 9.285305400658607E-5 0.0 7 0.0 2.3213263501646517E-5 0.0 1.6249284451152563E-4 0.0 8 0.0 2.3213263501646517E-5 0.0 1.6249284451152563E-4 0.0 9 0.0 2.3213263501646517E-5 0.0 2.5534589851811167E-4 0.0 10 0.0 2.3213263501646517E-5 0.0 2.5534589851811167E-4 0.0 11 0.0 2.3213263501646517E-5 0.0 3.0177242552140474E-4 0.0 12 0.0 2.3213263501646517E-5 0.0 3.0177242552140474E-4 0.0 13 0.0 2.3213263501646517E-5 0.0 3.2498568902305125E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTCGC 35 0.0021759737 16.28018 8 GACCGTT 75 2.0817794E-4 11.396257 7 GAACGAT 100 2.3882638E-5 10.45507 16 ATCGCCG 175 2.3646862E-10 10.311739 19 AAATCGC 185 6.002665E-11 10.2753725 17 GCGACTA 880 0.0 10.260657 16 CCGGTTG 75 0.0026388164 10.138131 14 GTACTGG 1905 0.0 10.023825 1 AATCGCC 190 9.822543E-11 10.005782 18 TAGCACG 95 1.650624E-4 9.996834 4 ACCGGGG 410 0.0 9.968132 15 TCGGAAC 105 4.0869585E-5 9.95721 13 CCGTACA 890 0.0 9.931897 17 GGACCGT 125 2.5833833E-6 9.876871 6 CCCCGTA 860 0.0 9.7255335 15 TAGACAG 465 0.0 9.59911 5 CGACTAA 935 0.0 9.555547 17 GAAATCG 220 1.6370905E-11 9.504609 16 CCCGTAC 900 0.0 9.504609 16 TGTCCCG 990 0.0 9.496551 11 >>END_MODULE