FastQCFastQC Report
Wed 25 May 2016
SRR1294663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307882
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC371080.861397782019099No Hit
GTACATGGGAAGCAGTGGTATCAAC337960.7845154533016457No Hit
GTATCAACGCAGAGTACTTTTTTTT313830.7285018484721726No Hit
GGTATCAACGCAGAGTACTTTTTTT221690.5146148385680016No Hit
TATCAACGCAGAGTACTTTTTTTTT193900.45010517929692595No Hit
GAGTACATGGGAAGCAGTGGTATCA173110.4018448044770029No Hit
CATGTACTCTGCGTTGATACCACTG144940.3364530411928646No Hit
ACGCAGAGTACTTTTTTTTTTTTTT124540.28909798364950573No Hit
GCGTTGATACCACTGCTTCCCATGT117370.27245407371882513No Hit
ACGCAGAGTACATGGGAAGCAGTGG96320.22359015404785926No Hit
GTATCAACGCAGAGTACATGGGAAG93800.21774041164544433No Hit
ACTCTGCGTTGATACCACTGCTTCC80610.18712211708677257No Hit
TATCAACGCAGAGTACATGGGAAGC80530.1869364109787594No Hit
ATTCCATTCCATTCCATTCCATTCC76940.1786028493816683No Hit
GGTATCAACGCAGAGTACATGGGAA72330.16790153490740925No Hit
GTACTTTTTTTTTTTTTTTTTTTTT68880.1598929589993412No Hit
GAATGGAATGGAATGGAATGGAATG66800.15506460019099874No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65510.15207008919928633No Hit
GCTTCCCATGTACTCTGCGTTGATA64660.15009696180164636No Hit
GTACTGGTTCACTATCGGTCAGTCA60960.14150805430603716No Hit
GTGGTATCAACGCAGAGTACATGGG52930.12286780371421502No Hit
CAGTGGTATCAACGCAGAGTACATG52440.12173035380263433No Hit
GGATACCACGTGTCCCGCCCTACTC48300.11212006271295269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA402.7166228E-416.661577
TCGTTGG854.032496E-612.2708213
CCGTGCA752.0348345E-411.4242779
CCGTACA9000.011.06258717
CCCCGTA9200.010.61559115
ATCGCCG1701.4370016E-1010.59777319
AATCGCC1803.6379788E-1110.53579718
TACACCG1105.8954647E-610.3864345
CCCGTAC9750.010.30887216
CTGTCGC1951.4551915E-1110.2525579
GTACTGG17300.010.2238291
GCCCCGT8500.010.15131514
CTTACAC5200.010.0487473
TACTGGT16850.010.0362782
AACCGTG1356.2601794E-79.8735227
TGTCCCG10600.09.75703711
CCCCCGG7500.09.73507618
GTCGCCC2151.0913936E-119.70915811
CCCCGGT7450.09.545855519
CGCCCTA10650.09.52673416
Produced by FastQC (version 0.11.5)