##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294663_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4307882
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40087379366473	31.0	31.0	34.0	28.0	34.0
2	31.54273283251491	31.0	31.0	34.0	28.0	34.0
3	31.623696052955953	31.0	31.0	34.0	28.0	34.0
4	35.21394063254286	37.0	35.0	37.0	32.0	37.0
5	35.408543920190944	37.0	35.0	37.0	33.0	37.0
6	35.02686331705465	37.0	35.0	37.0	32.0	37.0
7	34.88961211100954	37.0	35.0	37.0	32.0	37.0
8	34.943484756546255	37.0	35.0	37.0	32.0	37.0
9	36.38091015492068	39.0	37.0	39.0	32.0	39.0
10	36.14856883266533	39.0	37.0	39.0	31.0	39.0
11	36.34010820166383	39.0	37.0	39.0	32.0	39.0
12	36.28564152871411	39.0	37.0	39.0	32.0	39.0
13	36.15121073418445	39.0	35.0	39.0	31.0	39.0
14	37.24458167609976	40.0	37.0	41.0	31.0	41.0
15	37.29408233558858	40.0	37.0	41.0	31.0	41.0
16	37.327305158312136	40.0	37.0	41.0	31.0	41.0
17	37.39885841812752	40.0	37.0	41.0	31.0	41.0
18	37.4001019526533	40.0	37.0	41.0	31.0	41.0
19	37.40786539649879	40.0	37.0	41.0	31.0	41.0
20	37.48106610162488	40.0	37.0	41.0	31.0	41.0
21	37.329806155321805	40.0	37.0	41.0	31.0	41.0
22	37.337881121163484	40.0	37.0	41.0	31.0	41.0
23	37.35021061393975	40.0	37.0	41.0	31.0	41.0
24	37.282788386497124	40.0	37.0	41.0	31.0	41.0
25	36.27203878843478	39.0	35.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5008211083266296
1101	2	0.48348788563454903
1101	3	0.425970956080036
1101	4	0.3438110364920348
1101	5	0.36255979939373617
1101	6	0.4254701209772094
1101	7	0.43766761969600765
1101	8	0.4253837169208765
1101	9	0.6175516011088291
1101	10	0.6770227521869074
1101	11	0.6631661005643039
1101	12	0.7274020580210063
1101	13	0.7095396994488112
1101	14	0.8940447432582417
1101	15	0.8392766125300994
1101	16	0.9469825614263527
1101	17	0.9064575503696233
1101	18	0.8743484566132622
1101	19	0.9128354868983592
1101	20	0.8716362815792564
1101	21	1.1105274013616793
1101	22	1.0530074394865991
1101	23	0.9920173611893119
1101	24	1.1273670551681576
1101	25	1.1123806610294906
1102	1	0.42876079542913814
1102	2	0.4240049903355292
1102	3	0.33224765506232856
1102	4	0.24439868230726347
1102	5	0.2946257802336163
1102	6	0.3849767191044009
1102	7	0.4340187809833864
1102	8	0.40735025676445247
1102	9	0.4801163968792963
1102	10	0.6563535404182659
1102	11	0.5824093121736738
1102	12	0.6608995111189984
1102	13	0.6785328865417455
1102	14	0.9006720597740809
1102	15	0.8115625055024722
1102	16	0.811753837054674
1102	17	0.711845551698481
1102	18	0.7557116687583303
1102	19	0.8707793687966401
1102	20	0.8522696885411207
1102	21	1.1457002239235408
1102	22	1.03670265855704
1102	23	0.9547175338052298
1102	24	1.0654281523038165
1102	25	0.9871266800415768
1103	1	0.33816378672828407
1103	2	0.2831621081993241
1103	3	0.25413316635907535
1103	4	0.24700471685977732
1103	5	0.2587105258306934
1103	6	0.29565251480388355
1103	7	0.367559502201523
1103	8	0.3506128053209565
1103	9	0.3012639696901971
1103	10	0.4969809324484942
1103	11	0.5434723440452913
1103	12	0.5505427844118032
1103	13	0.616424091438617
1103	14	0.7716340883111741
1103	15	0.7486976782786456
1103	16	0.7198243652422178
1103	17	0.6877236171335213
1103	18	0.7214137194043673
1103	19	0.8666690398396426
1103	20	0.7926182925727332
1103	21	0.9539650946243654
1103	22	0.8682931336631086
1103	23	0.9076763984988219
1103	24	1.0128486715596026
1103	25	1.0383698198579623
1104	1	0.1484937510292852
1104	2	0.1184089058964446
1104	3	0.09225759027374991
1104	4	0.09370944021080163
1104	5	0.13632382718366642
1104	6	0.07067930734366712
1104	7	0.17555489214727515
1104	8	0.062148708238694894
1104	9	-0.13469174443614662
1104	10	0.3398722338343916
1104	11	0.31606334723844753
1104	12	0.33284019669138587
1104	13	-0.10743415087932817
1104	14	0.21922958359824918
1104	15	0.42219354692387157
1104	16	0.40683789318875796
1104	17	0.36695707845533576
1104	18	0.45641246893344345
1104	19	0.5292089428927085
1104	20	0.43311127301875274
1104	21	0.6647664315995314
1104	22	0.6181009909183075
1104	23	0.5210599922288139
1104	24	0.6424756997457237
1104	25	0.6171117683797078
1105	1	-0.004560394954250313
1105	2	0.006939497293853236
1105	3	-0.01703349139850019
1105	4	-0.04231304453761453
1105	5	0.020166779411681546
1105	6	-0.06925950039109807
1105	7	-0.013185227896954643
1105	8	-0.08073416493979835
1105	9	0.1605054200857623
1105	10	0.231024126619495
1105	11	0.16326266567045877
1105	12	-0.2552156217215398
1105	13	-0.5115224316782729
1105	14	-0.8543638312422672
1105	15	-0.23174281500274674
1105	16	0.07882994163965407
1105	17	0.01847691500172033
1105	18	-0.7201359691476554
1105	19	-0.6444023727813715
1105	20	-0.5543919000071256
1105	21	0.03660602084134723
1105	22	0.12003535290174483
1105	23	0.2075467046800128
1105	24	0.3444484488785875
1105	25	0.13053894774809294
1106	1	-0.37088484593676085
1106	2	-0.3790217480207936
1106	3	-0.5226082458546664
1106	4	-0.39398544755596276
1106	5	-0.3580450945031757
1106	6	-0.3231672150845526
1106	7	-0.3264245909953587
1106	8	-0.2293799595477637
1106	9	-0.18467759351758417
1106	10	-1.2156561680464293
1106	11	-0.9749227150940385
1106	12	-1.5209348076526155
1106	13	-0.5652476957436647
1106	14	-0.7834711356633264
1106	15	-0.5681245262343992
1106	16	-0.666055594492704
1106	17	-0.9471588906259001
1106	18	-0.5037328182183174
1106	19	-0.27081968803553735
1106	20	-0.40367946977941926
1106	21	-0.10799462981150043
1106	22	-0.24111935270548912
1106	23	-0.5841544567146713
1106	24	-0.3131755189445471
1106	25	-0.5733940841769325
1107	1	0.12932201248602126
1107	2	0.16365877651513827
1107	3	0.05567342609555226
1107	4	-0.014259379330425759
1107	5	0.11357267512886438
1107	6	0.08180293511279046
1107	7	0.1491618089261948
1107	8	0.11900762210857607
1107	9	0.27582678557168094
1107	10	0.26459040087132735
1107	11	0.3003102062642782
1107	12	-0.15096368009519523
1107	13	0.1022864344445722
1107	14	0.15491942323181718
1107	15	0.26809780931428406
1107	16	0.16837455942054902
1107	17	0.19957232373916867
1107	18	0.22238448499039265
1107	19	0.3249960012065358
1107	20	0.24658582058361844
1107	21	0.5577889775052114
1107	22	0.5983798002491056
1107	23	0.5307342371322861
1107	24	0.5674753157790278
1107	25	0.5282823947978912
1108	1	0.09258832280772111
1108	2	0.12097534440847113
1108	3	0.027698735784909445
1108	4	0.0314074439637011
1108	5	0.09602082992440586
1108	6	0.11220336685368437
1108	7	0.1011829216533755
1108	8	0.08117847394951383
1108	9	0.06701858044060316
1108	10	-0.7222372536080428
1108	11	-0.052119071538442086
1108	12	0.14535515457787795
1108	13	0.2745126754487046
1108	14	0.274947406091961
1108	15	0.021461856567690063
1108	16	0.07932893414670872
1108	17	0.2313142427779482
1108	18	0.1997381626193473
1108	19	0.23259934206311783
1108	20	0.2628475317537209
1108	21	0.5322778433006974
1108	22	0.40038122366820517
1108	23	0.3540995518776029
1108	24	0.5551078159523399
1108	25	0.3952403278383656
1109	1	-0.0024815875866117665
1109	2	-0.03160245638678205
1109	3	-0.06906850031947087
1109	4	-0.14844441802403452
1109	5	-0.020911533830158646
1109	6	-0.06229409730703139
1109	7	-0.24367037631777322
1109	8	-0.18205133976086785
1109	9	0.06511417389558005
1109	10	-0.3966890767159086
1109	11	0.027714480790500318
1109	12	0.10645747002418204
1109	13	0.1847277082150498
1109	14	0.04704984184171934
1109	15	-3.6745247079949763
1109	16	-1.716729901217839
1109	17	-1.5172849140713467
1109	18	-0.7835563520441227
1109	19	-0.41849369460560126
1109	20	-0.4273727529757352
1109	21	0.09111011597381946
1109	22	0.17257939164576186
1109	23	0.1510414361633181
1109	24	0.2431537928665577
1109	25	0.15156964708421583
1110	1	-0.13412241683915838
1110	2	-0.09512836952663761
1110	3	-0.11716201873007392
1110	4	-0.12455123423857373
1110	5	-0.052777208090262206
1110	6	-0.905375981499887
1110	7	-0.8810161670052068
1110	8	-0.9718152004161951
1110	9	-0.28680972749040734
1110	10	-0.09339477751917968
1110	11	0.026298087167120343
1110	12	-0.011282680511769172
1110	13	0.08644562848036941
1110	14	0.09883263790079155
1110	15	0.08097068477169245
1110	16	0.13297687529371416
1110	17	0.056487090492510106
1110	18	-0.120121189811627
1110	19	0.1537868880156381
1110	20	0.06872889084966971
1110	21	-1.0505179218866871
1110	22	-1.0141308486049283
1110	23	-1.192433439576881
1110	24	-1.0210524345834315
1110	25	-0.8110315156850092
1111	1	-0.3464299415280898
1111	2	-0.31028726255909334
1111	3	-0.30804417716246135
1111	4	-0.1472676343256012
1111	5	-0.22369409213698077
1111	6	-0.2269371571636114
1111	7	-0.33558724142287133
1111	8	-0.3714626456859307
1111	9	-0.3234448863304493
1111	10	-0.15795206567587883
1111	11	-0.5552506434404663
1111	12	-0.3209099481000166
1111	13	-0.22015174149975536
1111	14	-0.3664506891349504
1111	15	-0.4521256802983018
1111	16	-0.5722136867307128
1111	17	-0.6832065120929371
1111	18	-1.0366281758329592
1111	19	-0.5115826404068144
1111	20	-0.484139317065555
1111	21	-0.06418248093000045
1111	22	-0.19324239478889638
1111	23	-1.2589082201243116
1111	24	-0.9322896115890202
1111	25	-1.6050510005762533
1112	1	-0.2572758652013718
1112	2	-0.3921544843257294
1112	3	-0.2971261890790906
1112	4	-0.24114254862308115
1112	5	-0.20510058989155766
1112	6	-0.15842534206736758
1112	7	-0.3297814042410323
1112	8	-0.18889660786997808
1112	9	-0.4467133416473317
1112	10	-0.2512848872115683
1112	11	-0.10270631339183467
1112	12	-0.3587783981809949
1112	13	-0.025321593458158986
1112	14	-0.7898917169566957
1112	15	-0.34212443500265266
1112	16	-0.34501130000839453
1112	17	-0.30095140903411277
1112	18	-0.21679330486610837
1112	19	-1.1220646335024966
1112	20	-0.8439003830386298
1112	21	-0.6311353415368401
1112	22	-0.9161250914766228
1112	23	-0.5338592042970305
1112	24	-0.9318494066529794
1112	25	-0.7358319141562788
1113	1	-0.2586779233047345
1113	2	-0.1953656773707948
1113	3	-0.2771473302520491
1113	4	-0.1550723363756461
1113	5	-0.13700525377709738
1113	6	-0.16100589462363502
1113	7	-0.22899444017451742
1113	8	-0.199738548327403
1113	9	-0.6113843381899144
1113	10	-0.2821558121270442
1113	11	-0.044640648982017694
1113	12	-0.10895200300787167
1113	13	-0.18105162865285962
1113	14	-0.26157486391887375
1113	15	-0.17208691000448084
1113	16	-0.49906433676257933
1113	17	-0.34674680271380964
1113	18	-0.16710370846167422
1113	19	-0.3002369812686183
1113	20	-0.4790993434280395
1113	21	-10.28366475927718
1113	22	-2.9167299768991057
1113	23	-1.9527751578758554
1113	24	-1.0077227227528809
1113	25	-1.945527820885573
1201	1	0.3955029237757728
1201	2	0.3688139241444155
1201	3	0.42529158270276923
1201	4	0.2653308545597497
1201	5	0.2756331761607669
1201	6	0.370738095867047
1201	7	0.45723934793125665
1201	8	0.41918498273503957
1201	9	0.5928520254062306
1201	10	0.6344939787697754
1201	11	0.6223650204376341
1201	12	0.6484245771457822
1201	13	0.6216709269023681
1201	14	0.8977767953182578
1201	15	0.8078551792181159
1201	16	0.8322593498086519
1201	17	0.8118375715208188
1201	18	0.7818804388405738
1201	19	0.8963875138979702
1201	20	0.8895245616777316
1201	21	1.1092150616726215
1201	22	0.9904470725420538
1201	23	0.9989652126191828
1201	24	1.1015074805198637
1201	25	1.0468658787354883
1202	1	0.41730591397972816
1202	2	0.44258536359140876
1202	3	0.4501590233899151
1202	4	0.3194371827288762
1202	5	0.31283210234523295
1202	6	0.38824278040301863
1202	7	0.4360395924328486
1202	8	0.4849988317794356
1202	9	0.6708912913865674
1202	10	0.6530298373763799
1202	11	0.6128295960450743
1202	12	0.6153870277608604
1202	13	0.7127473458482925
1202	14	0.8883265043238922
1202	15	0.8485774581812819
1202	16	0.8024488942545176
1202	17	0.8239703049121943
1202	18	0.794966323236423
1202	19	0.849660434918249
1202	20	0.7280935623482776
1202	21	1.106411509063392
1202	22	1.0785245733433229
1202	23	1.0337100040370757
1202	24	1.0489498706305582
1202	25	1.0594948693213837
1203	1	0.3147825835816569
1203	2	0.30196178819164743
1203	3	0.2962277804768725
1203	4	0.25886867019941207
1203	5	0.21224520018119364
1203	6	0.2692969933581111
1203	7	0.3299247523602702
1203	8	0.30835982908969584
1203	9	0.4177498040399712
1203	10	0.48660205398289236
1203	11	0.5452247914987041
1203	12	0.4446246553260522
1203	13	0.5392404701897462
1203	14	0.6575483400733901
1203	15	0.6568097928875289
1203	16	0.6576044005093067
1203	17	0.640446494712883
1203	18	0.4862528618705966
1203	19	0.7132931844301211
1203	20	0.7265142518191468
1203	21	0.9616528307470915
1203	22	0.8634972156453742
1203	23	0.7976167568114789
1203	24	0.9191711215484162
1203	25	0.8899683164386047
1204	1	0.21568577308488557
1204	2	0.21491555941399199
1204	3	0.22500922800664824
1204	4	0.21798288095484963
1204	5	0.16470772802581735
1204	6	0.2760775132672535
1204	7	0.2608721631458266
1204	8	0.29885556529274027
1204	9	0.02601135249614117
1204	10	0.343940314968215
1204	11	0.3984815936125159
1204	12	0.4724371355706083
1204	13	0.3371193129236687
1204	14	0.4724419948907723
1204	15	0.54987555894661
1204	16	0.48852171288925916
1204	17	0.547616855500344
1204	18	0.6141966459600994
1204	19	0.6930174887883567
1204	20	0.6350905857269069
1204	21	0.8332881004974837
1204	22	0.7823891541704029
1204	23	0.7957512597897107
1204	24	0.850755323977701
1204	25	0.6462404034361597
1205	1	0.11357783861794957
1205	2	0.15152551146818638
1205	3	0.18668623267294038
1205	4	0.017298176795144116
1205	5	0.06699923233563254
1205	6	0.10080635735527466
1205	7	-0.014410332247969393
1205	8	-0.027747788038290366
1205	9	0.12226655129465769
1205	10	0.14771317733212186
1205	11	-0.10734569089125046
1205	12	-0.130558787966784
1205	13	0.31648593872003516
1205	14	-0.041196983435185075
1205	15	0.20013728863784053
1205	16	0.27801185646125504
1205	17	0.1181611727463121
1205	18	0.012334228232170119
1205	19	0.07645311584769843
1205	20	0.3081925385803217
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2111	21	0.021918126021837736
2111	22	-0.1485727688545566
2111	23	-1.2547033953536726
2111	24	-1.0105080998507745
2111	25	-1.161383689957134
2112	1	-0.30747647080132623
2112	2	-0.21110114459900586
2112	3	-0.23000945367926917
2112	4	-0.10561580879247146
2112	5	-0.21106012955345932
2112	6	-0.43826704239391034
2112	7	-0.5151312253745033
2112	8	-0.512461686800421
2112	9	-0.3255715344853769
2112	10	-0.39377074908046694
2112	11	-0.1887410681995192
2112	12	-0.06311884292940562
2112	13	0.04767975687461501
2112	14	-0.8823476546412579
2112	15	-1.1653999057715012
2112	16	-1.2999963927649318
2112	17	-0.7185467239426373
2112	18	-1.5864493451599273
2112	19	-1.2343354898784398
2112	20	-0.8498490232796172
2112	21	-0.5622477139336297
2112	22	-1.0580495276900166
2112	23	-0.5333450504848258
2112	24	-0.3220711247828447
2112	25	-0.41429788957463387
2113	1	-0.22629466766351314
2113	2	-0.31349429227987713
2113	3	-0.18082098102109256
2113	4	-0.06530844036954875
2113	5	-0.12476130360360571
2113	6	-0.021611189505371442
2113	7	-0.15441151603365455
2113	8	-0.022843008799519282
2113	9	-0.30869798741859
2113	10	-0.002878350511011263
2113	11	0.040598931614290734
2113	12	1.492638475184549E-4
2113	13	0.07092269842238608
2113	14	-0.40437055502511754
2113	15	-0.06145533716651386
2113	16	0.05112555809004249
2113	17	-0.25911213476076256
2113	18	-0.15580796810460384
2113	19	-0.6412996643223181
2113	20	-0.017329525705164883
2113	21	0.10311420627924406
2113	22	-1.9715376560615638
2113	23	-2.64750257547162
2113	24	-1.53898297447342
2113	25	-1.7586799592602063
2114	1	-0.7515489094448817
2114	2	-0.8814317128015894
2114	3	-0.5655117999489185
2114	4	-0.37039004342979354
2114	5	-0.45846789270074595
2114	6	-0.31512564025105405
2114	7	-0.5994281550686722
2114	8	-0.36376479343940815
2114	9	-0.8571851724424988
2114	10	-0.8187785173583038
2114	11	-0.384525410816984
2114	12	-0.8968427946867195
2114	13	-0.4042250914495682
2114	14	-1.235207900933439
2114	15	-0.6043545616600667
2114	16	-3.650227233484678
2114	17	-3.110798783299373
2114	18	-1.9305929023177129
2114	19	-2.323119366985054
2114	20	-1.4975488206414909
2114	21	-1.7716285006377177
2114	22	-2.456442889353589
2114	23	-3.402554877727823
2114	24	-7.677631630001173
2114	25	-4.007258630465152
2201	1	0.5251866874916438
2201	2	0.5373367265199356
2201	3	0.48087457857281635
2201	4	0.3421860145696982
2201	5	0.3375511746856503
2201	6	0.49102465220845914
2201	7	0.5210661815908324
2201	8	0.5334610877637047
2201	9	0.733635152896305
2201	10	0.9510601309217819
2201	11	0.8413015425185719
2201	12	0.8488490821217312
2201	13	0.844954625789768
2201	14	1.1066770272424762
2201	15	1.0204056935458183
2201	16	1.0254064263109228
2201	17	0.943448807973212
2201	18	1.0144719255112378
2201	19	1.1043837344961318
2201	20	1.061463712420057
2201	21	1.269924064090013
2201	22	1.2007120267928784
2201	23	1.2208920473537788
2201	24	1.338588175376401
2201	25	1.4124156213846817
2202	1	0.4682546962312877
2202	2	0.5377178959456934
2202	3	0.44574092279141553
2202	4	0.3321245469625822
2202	5	0.30724783703085023
2202	6	0.4473242886611075
2202	7	0.4886823626210983
2202	8	0.542192173782091
2202	9	0.6655583047883837
2202	10	0.8392069913594895
2202	11	0.7561101475302721
2202	12	0.7604455400282077
2202	13	0.8365866338222574
2202	14	1.0757282457153892
2202	15	0.9765666199116154
2202	16	0.9591130907413046
2202	17	0.9062223184263303
2202	18	0.8774980945743565
2202	19	1.0252339910644608
2202	20	0.9214976986578449
2202	21	1.1333273795330712
2202	22	1.1000750823131753
2202	23	1.0605830207722136
2202	24	1.2439500455147439
2202	25	1.2722964485682269
2203	1	0.190615038821214
2203	2	0.3201622309934393
2203	3	-0.2992316609512393
2203	4	-0.028251720446476725
2203	5	0.09025200921074372
2203	6	0.2768482807361323
2203	7	0.36715133331732375
2203	8	0.41091288541981186
2203	9	0.4462347619903966
2203	10	0.7084063510791907
2203	11	0.5888883582975524
2203	12	0.5953676258423144
2203	13	0.6236434610909711
2203	14	0.7438168255163902
2203	15	0.6999330368329382
2203	16	0.7549839048750968
2203	17	0.731935505616562
2203	18	0.791181313449286
2203	19	0.8735992610092538
2203	20	0.8510475856630677
2203	21	1.0867733332954188
2203	22	0.9561399705047791
2203	23	0.9636732147843503
2203	24	1.1309545762831306
2203	25	1.1633037303914833
2204	1	0.21578559210136916
2204	2	0.21441293715784582
2204	3	0.18344364426038595
2204	4	0.10710816416220581
2204	5	0.13053645484674803
2204	6	0.21069591638364926
2204	7	0.3055356212507192
2204	8	0.22665690098546065
2204	9	0.386580072859239
2204	10	0.5957287401892657
2204	11	0.4185766359892469
2204	12	0.4189948242322856
2204	13	0.4913197154785962
2204	14	0.6578141903044425
2204	15	0.5521399621534755
2204	16	0.5597277176401079
2204	17	0.5710742820478742
2204	18	0.5499757149719073
2204	19	0.7031775550360209
2204	20	0.5856776188938255
2204	21	0.9082608305065278
2204	22	0.8099556816754898
2204	23	0.8301265164797798
2204	24	0.9382584515226498
2204	25	0.945438304378392
2205	1	0.014283378460714147
2205	2	0.01975867116476593
2205	3	-0.14054752001923632
2205	4	-0.08251516553232108
2205	5	-0.12448887799241248
2205	6	-0.01796345968316615
2205	7	0.06655569946516948
2205	8	0.09520816642376673
2205	9	0.18747941613942487
2205	10	0.3847352225129441
2205	11	0.016431977644053575
2205	12	0.30618104421619563
2205	13	0.08133039678304499
2205	14	0.10204109584692844
2205	15	0.25945752610061845
2205	16	0.3951368198123646
2205	17	0.3837598447888837
2205	18	0.39546960978846357
2205	19	0.4874250481242157
2205	20	0.41744441621600714
2205	21	0.752467026450546
2205	22	0.8030553132083114
2205	23	0.6300830021374111
2205	24	0.720958841424995
2205	25	0.6172609129998037
2206	1	-0.5472709293076363
2206	2	-0.4872854738415384
2206	3	-0.23350289695431314
2206	4	-0.26770148172543173
2206	5	-0.2962283645320838
2206	6	-0.22920535289026844
2206	7	-0.17325816402021843
2206	8	-0.17708220300075084
2206	9	-0.24730283482528392
2206	10	-0.05138391776073803
2206	11	-0.6482180458832616
2206	12	-0.36416316156859097
2206	13	-1.3233307951042548
2206	14	-0.7956544892361705
2206	15	-0.43027140392906915
2206	16	-0.21007754118570432
2206	17	-0.1849222032025395
2206	18	-0.22953428053552472
2206	19	-0.1886338281446811
2206	20	-0.2301875496712924
2206	21	0.03397012210776751
2206	22	-0.12658151476281887
2206	23	-0.15337847529723092
2206	24	0.12005560982350971
2206	25	0.1831184638235328
2207	1	0.19689575906709322
2207	2	0.24290355914436645
2207	3	0.23795448477264003
2207	4	0.20407018684653622
2207	5	0.179429308400195
2207	6	0.3097912010380739
2207	7	0.300082089593424
2207	8	0.2833602983493222
2207	9	0.39603638807277974
2207	10	0.6164859287866307
2207	11	0.5228637696389384
2207	12	0.4471121592181504
2207	13	0.48544494648495373
2207	14	0.5077713626133047
2207	15	0.5088743282321673
2207	16	0.5801205540819723
2207	17	0.6095493227474478
2207	18	0.559725932440621
2207	19	0.6456052845933087
2207	20	0.5794398785843953
2207	21	0.889947971867258
2207	22	0.7620698934726917
2207	23	0.608297947891792
2207	24	0.8685899711004623
2207	25	0.7869050723860624
2208	1	0.16900531372242256
2208	2	0.1327531166073328
2208	3	0.14786276863808823
2208	4	0.06972518510847436
2208	5	0.11704921788733458
2208	6	0.1939837046478914
2208	7	0.17786674719543782
2208	8	0.1427073055422241
2208	9	0.3078577922922605
2208	10	0.3287273922338727
2208	11	0.05607744416698779
2208	12	-0.19981331139525338
2208	13	0.04674167358266601
2208	14	-0.41990483330626205
2208	15	0.18492109209654473
2208	16	0.24299565769713638
2208	17	0.1863147914890888
2208	18	0.3346988588003228
2208	19	0.41686644829190556
2208	20	0.37033360953257244
2208	21	0.6604341108319147
2208	22	0.6272586234849911
2208	23	0.6112802263558805
2208	24	0.6489049277826382
2208	25	0.681726904435898
2209	1	-0.015574434698514494
2209	2	0.04328056307018002
2209	3	0.08121808245619988
2209	4	-0.5005278742014809
2209	5	-0.10742908679953445
2209	6	0.09870884699769533
2209	7	0.18627083213821294
2209	8	0.1991631468304007
2209	9	0.22370435958086432
2209	10	0.26144958702324317
2209	11	0.2760628972317747
2209	12	0.2825955537385667
2209	13	0.33118654012356075
2209	14	0.3980217271439699
2209	15	-0.24532374454186368
2209	16	-0.27375498273332255
2209	17	0.1354102155256811
2209	18	-0.23497927794415574
2209	19	0.20022511767421491
2209	20	0.24250977911601268
2209	21	0.5634495188233686
2209	22	0.5216879430915
2209	23	0.4987035358082679
2209	24	0.618402310442562
2209	25	0.5203013956010167
2210	1	-0.12203139772550387
2210	2	-0.09719286708131847
2210	3	-0.05850845044661668
2210	4	-0.0017275917495709336
2210	5	-0.052625250082989794
2210	6	-0.4652729384132783
2210	7	-0.1067157223463866
2210	8	-0.2956556365872487
2210	9	-0.05374620676385433
2210	10	0.14136025551636777
2210	11	0.0032607631690027006
2210	12	0.08826542891463163
2210	13	0.20079996993460014
2210	14	0.17035394071517374
2210	15	0.06934437279110028
2210	16	0.18752467100220116
2210	17	-0.9315388008952112
2210	18	-1.4307629062253255
2210	19	-0.3497614867460328
2210	20	-0.12237455907293793
2210	21	-0.326649812432926
2210	22	-0.12381347149872113
2210	23	-0.07817854185889672
2210	24	0.2763797058942643
2210	25	0.2568416836923859
2211	1	-0.18951480943968235
2211	2	-0.19213468620178986
2211	3	-0.14001598935133686
2211	4	-0.019558996813721308
2211	5	-0.06118399280995135
2211	6	-0.024963482942524706
2211	7	-0.1622740787731587
2211	8	-0.030182031039487356
2211	9	-0.0014773016739155764
2211	10	0.10132768748098187
2211	11	-0.20037327784042702
2211	12	-0.055192118702123594
2211	13	-0.0496727836624018
2211	14	-0.1477692316991721
2211	15	-0.1878860487409355
2211	16	-0.12123739847214665
2211	17	-0.2765713641173164
2211	18	-0.3757242191343977
2211	19	-0.155623458070707
2211	20	-0.3417689740440579
2211	21	0.1706807556016301
2211	22	-0.059605222552519876
2211	23	-0.6659149753853555
2211	24	-0.09475687618380135
2211	25	-0.7234027264723295
2212	1	-0.2344292487985591
2212	2	-0.32622541301031305
2212	3	-0.14578468745150985
2212	4	-0.1213691530569676
2212	5	-0.10580728179838417
2212	6	-0.12877816401733355
2212	7	-0.1194243770643908
2212	8	-0.3316552101719523
2212	9	-0.09726303050543805
2212	10	-0.528434007506128
2212	11	-0.16614775422445405
2212	12	-0.40447803254009784
2212	13	-0.3458383347744203
2212	14	-0.2357543071493069
2212	15	-0.08156880127921795
2212	16	-0.3183217056609777
2212	17	-0.5774022980317
2212	18	-0.4231626921886047
2212	19	-0.43332591640549367
2212	20	-0.5775836151658211
2212	21	0.003573010286203271
2212	22	-0.3632449599715599
2212	23	-0.23030539178255793
2212	24	-0.4567522416304399
2212	25	-0.2790055747071065
2213	1	-0.24509938978217605
2213	2	-0.2515593614141096
2213	3	-0.1469112918020059
2213	4	-0.0035362993886920435
2213	5	-0.10487837866867267
2213	6	-0.287983941981814
2213	7	-0.3903258742758382
2213	8	-0.32234404244472614
2213	9	-0.44602522239877374
2213	10	-0.3695567138093878
2213	11	-0.1597519797890783
2213	12	-0.018300948223455293
2213	13	0.005611143403768892
2213	14	-0.661899670216016
2213	15	-0.25852112627865154
2213	16	-0.4199100454109086
2213	17	-0.24000958020477725
2213	18	-0.3241556001343824
2213	19	-0.4280390626206838
2213	20	-0.44910962687065137
2213	21	-0.1353574515535172
2213	22	-0.4833680436118968
2213	23	-0.5890156190422786
2213	24	-0.7667202125060157
2213	25	-0.573147015809063
2214	1	-0.3242430888319916
2214	2	-0.19993320206080156
2214	3	-0.19564238967256387
2214	4	-0.09171118276229606
2214	5	-0.19547005314515076
2214	6	-0.15936045795135811
2214	7	-0.23454547295871464
2214	8	-0.15309501841543494
2214	9	-0.2275686328972455
2214	10	-0.16654692886346822
2214	11	-0.07085598513491931
2214	12	-0.2594264052944979
2214	13	-0.18310000639214508
2214	14	-0.28600612653897883
2214	15	-0.11806106796478844
2214	16	-0.3728525934068756
2214	17	-0.3754586495839618
2214	18	-0.37026245386977763
2214	19	-0.45334379222932597
2214	20	-0.5500245434848168
2214	21	-0.5559728479203443
2214	22	-0.8423387747733315
2214	23	-0.9809570268811854
2214	24	-0.6069477257858154
2214	25	-0.8360760736825128
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	32.0
9	76.0
10	153.0
11	308.0
12	765.0
13	1563.0
14	3329.0
15	5854.0
16	8764.0
17	11473.0
18	13448.0
19	15025.0
20	16404.0
21	17696.0
22	18884.0
23	20910.0
24	23619.0
25	26520.0
26	30624.0
27	34671.0
28	41472.0
29	52125.0
30	67421.0
31	88816.0
32	121467.0
33	166508.0
34	231429.0
35	328327.0
36	476178.0
37	772166.0
38	1711851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27190747340553	21.861048387509022	11.835637777045594	24.031406362039856
2	13.982950322223312	23.181670250020776	39.3444388681027	23.49094055965321
3	17.933746577509314	29.157656442022965	30.188860817046358	22.71973616342136
4	11.627303369821371	18.27285308044989	39.05151065401042	31.048332895718318
5	12.495899053920736	38.85370401629187	35.12889566659151	13.521501263195876
6	29.661105485079254	39.10269357689795	17.89519236617899	13.341008571843807
7	23.943148231131254	34.619722822667335	24.636068344714058	16.80106060148735
8	23.918699481458678	37.39471410110139	22.26509734432732	16.421489073112607
9	24.906357564609564	16.022536396126537	22.842858058124236	36.22824798113966
10	15.185968107768124	30.198881928550904	33.40719095792516	21.207959005755814
11	31.42128836211242	26.893861086424593	22.754601873966052	18.930248677496937
12	20.515299005601197	28.888124389338575	32.54052099096784	18.05605561409239
13	26.689896958620725	24.367502259231394	27.37445625819283	21.56814452395505
14	20.005334440148417	23.74127640292526	29.63290787807206	26.620481278854264
15	24.258537921556975	29.160994837319866	25.034355964938342	21.546111276184817
16	20.517442934754854	31.40294660291986	27.556944536008	20.522665926317284
17	19.765819900781846	30.60026495625111	29.79464845941659	19.839266683550452
18	20.08209621827204	29.238078568722564	31.083769434189318	19.596055778816083
19	20.121517102067354	28.78948789320757	31.745147428973613	19.343847575751454
20	21.92761950038917	27.333419532098556	30.382488063134637	20.356472904377636
21	21.54518018520973	29.243364416731875	29.705564671939026	19.50589072611936
22	20.208947289616358	28.874421541465047	30.19396279062209	20.7226683782965
23	19.609013815625147	29.425986263300057	30.740949832353575	20.224050088721217
24	20.157014587221557	28.598544991968204	30.39652915122984	20.847911269580397
25	21.138800593247666	27.640568427645306	29.178053922024933	22.042577057082095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3569.0
1	3569.0
2	3787.5
3	4006.0
4	4006.0
5	4006.0
6	8574.0
7	13142.0
8	13142.0
9	13142.0
10	17825.0
11	22508.0
12	22508.0
13	22508.0
14	31943.5
15	41379.0
16	41379.0
17	41379.0
18	66917.0
19	92455.0
20	92455.0
21	92455.0
22	134848.0
23	177241.0
24	177241.0
25	177241.0
26	230192.0
27	283143.0
28	283143.0
29	283143.0
30	334772.0
31	386401.0
32	386401.0
33	386401.0
34	428724.0
35	471047.0
36	471047.0
37	471047.0
38	489424.5
39	507802.0
40	507802.0
41	507802.0
42	502690.5
43	497579.0
44	497579.0
45	497579.0
46	548231.5
47	598884.0
48	598884.0
49	598884.0
50	532932.5
51	466981.0
52	466981.0
53	466981.0
54	383493.5
55	300006.0
56	300006.0
57	300006.0
58	245739.0
59	191472.0
60	191472.0
61	191472.0
62	161979.0
63	132486.0
64	132486.0
65	132486.0
66	99337.0
67	66188.0
68	66188.0
69	66188.0
70	49349.0
71	32510.0
72	32510.0
73	32510.0
74	22620.0
75	12730.0
76	12730.0
77	12730.0
78	8503.0
79	4276.0
80	4276.0
81	4276.0
82	2870.5
83	1465.0
84	1465.0
85	1465.0
86	925.5
87	386.0
88	386.0
89	386.0
90	274.0
91	162.0
92	162.0
93	162.0
94	101.5
95	41.0
96	41.0
97	41.0
98	32.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09675288227486267
2	0.0
3	0.13521725989709096
4	0.010283475731229407
5	0.022029387063062546
6	0.012163750074862775
7	0.020706231043468692
8	0.018570610801317215
9	1.392795810098791E-4
10	0.3886596708080676
11	0.06471857864259048
12	0.0012303029655872655
13	2.3213263501646517E-5
14	6.035448510428095E-4
15	9.749570670691537E-4
16	1.392795810098791E-4
17	2.3213263501646517E-5
18	2.3213263501646516E-4
19	2.5534589851811167E-4
20	2.0891937151481862E-4
21	2.0891937151481862E-4
22	0.002251686559659712
23	1.392795810098791E-4
24	4.6426527003293034E-5
25	0.0016017151816136099
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4307882.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.69582035259124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52151502727244	45.303632248268045
2	13.915419550249933	16.057230930043122
3	3.9923287372112233	6.9102204483187855
4	1.5353569985830142	3.543347262693571
5	0.6891210774374494	1.9879702942507602
6	0.3667785464394856	1.2696953474734263
7	0.21951333989318902	0.8865501556432315
8	0.138869047923017	0.6409730913201417
9	0.09769705421574591	0.5073040520108139
>10	0.4450877156648043	4.6721509943468025
>50	0.03774196266079962	1.520189425089638
>100	0.03249254565634012	4.025273541770716
>500	0.0035723564770443	1.4342213619104391
>1k	0.0036129512440745896	4.3760671871074095
>5k	5.277344513816781E-4	2.18455548685603
>10k+	3.653546201873156E-4	4.680618172897073
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	37108	0.861397782019099	No Hit
GTACATGGGAAGCAGTGGTATCAAC	33796	0.7845154533016457	No Hit
GTATCAACGCAGAGTACTTTTTTTT	31383	0.7285018484721726	No Hit
GGTATCAACGCAGAGTACTTTTTTT	22169	0.5146148385680016	No Hit
TATCAACGCAGAGTACTTTTTTTTT	19390	0.45010517929692595	No Hit
GAGTACATGGGAAGCAGTGGTATCA	17311	0.4018448044770029	No Hit
CATGTACTCTGCGTTGATACCACTG	14494	0.3364530411928646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12454	0.28909798364950573	No Hit
GCGTTGATACCACTGCTTCCCATGT	11737	0.27245407371882513	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	9632	0.22359015404785926	No Hit
GTATCAACGCAGAGTACATGGGAAG	9380	0.21774041164544433	No Hit
ACTCTGCGTTGATACCACTGCTTCC	8061	0.18712211708677257	No Hit
TATCAACGCAGAGTACATGGGAAGC	8053	0.1869364109787594	No Hit
ATTCCATTCCATTCCATTCCATTCC	7694	0.1786028493816683	No Hit
GGTATCAACGCAGAGTACATGGGAA	7233	0.16790153490740925	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6888	0.1598929589993412	No Hit
GAATGGAATGGAATGGAATGGAATG	6680	0.15506460019099874	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6551	0.15207008919928633	No Hit
GCTTCCCATGTACTCTGCGTTGATA	6466	0.15009696180164636	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6096	0.14150805430603716	No Hit
GTGGTATCAACGCAGAGTACATGGG	5293	0.12286780371421502	No Hit
CAGTGGTATCAACGCAGAGTACATG	5244	0.12173035380263433	No Hit
GGATACCACGTGTCCCGCCCTACTC	4830	0.11212006271295269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.3213263501646517E-5	0.0
5	0.0	0.0	0.0	2.3213263501646517E-5	0.0
6	2.3213263501646517E-5	0.0	0.0	2.3213263501646517E-5	0.0
7	2.3213263501646517E-5	0.0	0.0	4.6426527003293034E-5	0.0
8	2.3213263501646517E-5	0.0	0.0	4.6426527003293034E-5	0.0
9	2.3213263501646517E-5	0.0	0.0	4.6426527003293034E-5	0.0
10	2.3213263501646517E-5	0.0	0.0	4.6426527003293034E-5	0.0
11	2.3213263501646517E-5	0.0	0.0	4.6426527003293034E-5	0.0
12	2.3213263501646517E-5	2.3213263501646517E-5	0.0	4.6426527003293034E-5	0.0
13	2.3213263501646517E-5	2.3213263501646517E-5	0.0	6.963979050493954E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	40	2.7166228E-4	16.66157	7
TCGTTGG	85	4.032496E-6	12.27082	13
CCGTGCA	75	2.0348345E-4	11.424277	9
CCGTACA	900	0.0	11.062587	17
CCCCGTA	920	0.0	10.615591	15
ATCGCCG	170	1.4370016E-10	10.597773	19
AATCGCC	180	3.6379788E-11	10.535797	18
TACACCG	110	5.8954647E-6	10.386434	5
CCCGTAC	975	0.0	10.308872	16
CTGTCGC	195	1.4551915E-11	10.252557	9
GTACTGG	1730	0.0	10.223829	1
GCCCCGT	850	0.0	10.151315	14
CTTACAC	520	0.0	10.048747	3
TACTGGT	1685	0.0	10.036278	2
AACCGTG	135	6.2601794E-7	9.873522	7
TGTCCCG	1060	0.0	9.757037	11
CCCCCGG	750	0.0	9.735076	18
GTCGCCC	215	1.0913936E-11	9.709158	11
CCCCGGT	745	0.0	9.5458555	19
CGCCCTA	1065	0.0	9.526734	16
>>END_MODULE