FastQCFastQC Report
Wed 25 May 2016
SRR1294662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450464
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67740.276437442051791No Hit
GTACATGGGAAGCAGTGGTATCAAC57540.23481267221228305No Hit
GGTATCAACGCAGAGTACTTTTTTT51100.20853193517635843No Hit
CCCATGTACTCTGCGTTGATACCAC48810.19918676626141008No Hit
TATCAACGCAGAGTACTTTTTTTTT39530.16131638742703422No Hit
ATTCCATTCCATTCCATTCCATTCC29050.1185489768468339No Hit
GAGTACATGGGAAGCAGTGGTATCA28330.11561075779933923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT655.468979E-513.1502556
ATACCGT802.8759618E-511.8717586
CGAAGAA600.005862452611.08709914
AATACCG600.00589014711.0800825
CGCCCTA1054.0878553E-59.95657716
TGTCCCG1151.03049915E-59.91013611
GACTGAC1106.857926E-59.4976017
TAGACAG4000.09.4972135
TATTCCG1201.7070255E-59.4972135
GTCTTGC4350.09.3911181
CTAGGAC2054.2564352E-109.2659523
TTATACC4100.09.26557354
TCTACAC3000.09.1810143
TCAGGAC2700.09.1458383
GCGTGCA850.0074527378.9389189
TCTATAC3950.08.8964873
TAGGACT2900.08.8422324
GTGTAAG4850.08.8147271
GTCTAGA2502.5465852E-118.7402921
CCGCCCT1201.7574015E-48.71147115
Produced by FastQC (version 0.11.5)