##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2450464 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.053291947973936 31.0 30.0 33.0 26.0 34.0 2 30.28245956684122 31.0 30.0 34.0 26.0 34.0 3 30.219431911670608 31.0 30.0 34.0 25.0 34.0 4 33.86042602543845 35.0 35.0 37.0 28.0 37.0 5 34.175316593102366 37.0 35.0 37.0 30.0 37.0 6 34.26276941836321 37.0 35.0 37.0 30.0 37.0 7 34.15826063961764 37.0 35.0 37.0 30.0 37.0 8 34.02196726823981 37.0 35.0 37.0 28.0 37.0 9 35.18450260848557 39.0 35.0 39.0 27.0 39.0 10 35.2916749644149 39.0 35.0 39.0 28.0 39.0 11 35.513045284484896 39.0 35.0 39.0 29.0 39.0 12 35.30838486098959 39.0 35.0 39.0 27.0 39.0 13 35.177467614296724 39.0 35.0 39.0 27.0 39.0 14 36.085636842655106 39.0 36.0 41.0 27.0 41.0 15 36.08124338900714 39.0 36.0 41.0 27.0 41.0 16 36.198839077007456 39.0 36.0 41.0 27.0 41.0 17 36.176855077242514 39.0 36.0 41.0 27.0 41.0 18 36.06905345273385 39.0 35.0 41.0 27.0 41.0 19 36.36160580200321 39.0 36.0 41.0 27.0 41.0 20 36.472963487731306 39.0 36.0 41.0 27.0 41.0 21 36.51661685297152 39.0 36.0 41.0 27.0 41.0 22 36.51622509043185 39.0 36.0 41.0 27.0 41.0 23 36.49685855413505 39.0 36.0 41.0 27.0 41.0 24 36.51127174282095 39.0 36.0 41.0 27.0 41.0 25 35.31280769682803 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.779550846342218 1101 2 0.8090557194569321 1101 3 1.0636537213898336 1101 4 0.9087860769529001 1101 5 0.6165674035534323 1101 6 0.634350291035922 1101 7 0.6359906599898579 1101 8 0.8109741199924159 1101 9 0.9925929561146134 1101 10 0.9976451956286851 1101 11 0.6695559608233239 1101 12 0.9626265978459756 1101 13 0.9937078690152319 1101 14 1.0987915510943154 1101 15 1.2728587087526861 1101 16 1.1707558461586345 1101 17 1.143972374665502 1101 18 1.1699540532125425 1101 19 1.0902477781008528 1101 20 0.953058280577082 1101 21 0.8699750796273946 1101 22 0.8672186696422273 1101 23 0.8739577437457129 1101 24 0.8831961587280119 1101 25 0.7124350570249192 1102 1 0.8057574926736137 1102 2 0.7359584577663227 1102 3 1.0825707378405447 1102 4 0.9182743494107726 1102 5 0.7938564251140363 1102 6 0.6859286643880296 1102 7 0.613939491580787 1102 8 0.622238904342943 1102 9 1.043719869064006 1102 10 0.9615201076395294 1102 11 0.712063630001488 1102 12 0.9338895572362631 1102 13 0.9477505904654677 1102 14 1.1313164704919032 1102 15 1.2246986597394667 1102 16 1.1224404582676755 1102 17 1.1734400511435013 1102 18 1.2617708445848237 1102 19 0.9479397682634527 1102 20 0.1843674288420658 1102 21 0.5402977499787482 1102 22 0.5098071584882433 1102 23 0.6900226080468883 1102 24 0.8138307234749931 1102 25 0.6910875845948539 1103 1 0.6342106716999822 1103 2 0.5892445614692043 1103 3 1.034990252099604 1103 4 0.8111660617117451 1103 5 0.5559539097070356 1103 6 0.43182513144289203 1103 7 0.3400932390274036 1103 8 0.530373140304981 1103 9 0.645921673858993 1103 10 0.5633278505013308 1103 11 0.5678801401994065 1103 12 0.7574330453736593 1103 13 0.7031972286706605 1103 14 0.6671967415579658 1103 15 0.8717132395974829 1103 16 0.8597852178075343 1103 17 0.8312618987347378 1103 18 0.9202374971479159 1103 19 0.6586996098410154 1103 20 -0.20815250401567909 1103 21 0.12032431556209389 1103 22 -0.32119827601164275 1103 23 0.2552178789568771 1103 24 0.49671343607769103 1103 25 0.43294667255607067 1104 1 0.30977521594519786 1104 2 0.2697995309298662 1104 3 0.5299706792370564 1104 4 0.36351105952741136 1104 5 0.09060080423405736 1104 6 0.08757680184233863 1104 7 0.11743745159675711 1104 8 0.3594744818015059 1104 9 0.5539372246072602 1104 10 0.4742621683232713 1104 11 0.24434130087264094 1104 12 0.3837193158138774 1104 13 0.3862961786680046 1104 14 0.5563773872766689 1104 15 0.5974127809527303 1104 16 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0.16518163811024067 2207 9 -1.4992339781830708 2207 10 -0.3857016050427262 2207 11 -0.017204731202305368 2207 12 0.3730116501730478 2207 13 0.40945866207428594 2207 14 0.6550931614347064 2207 15 0.651394105774223 2207 16 0.6334604030390523 2207 17 0.6954253648245654 2207 18 0.7470262728380135 2207 19 -0.46452505282191936 2207 20 0.19064117409301673 2207 21 -0.39292767105275317 2207 22 0.02451793534113733 2207 23 -0.6266492641902346 2207 24 -1.7978541296370238 2207 25 -0.3445397940616459 2208 1 0.41590576685932135 2208 2 0.11139828748422786 2208 3 0.668672578621532 2208 4 0.5205221124874129 2208 5 0.44694672980861583 2208 6 0.2795108951360348 2208 7 0.36896419299586114 2208 8 0.5162356721365029 2208 9 -0.18131560637253585 2208 10 0.08002810275180394 2208 11 0.24508009323184865 2208 12 0.2622754905687188 2208 13 0.4920291908938026 2208 14 0.7973806021977481 2208 15 0.6452747414118036 2208 16 0.500105714676252 2208 17 0.5015070340233194 2208 18 0.7049066760896423 2208 19 -1.4592016341988057 2208 20 -0.20513972537096947 2208 21 -0.30124628554924726 2208 22 0.1304131315006316 2208 23 0.41219968303144583 2208 24 0.5061811682318549 2208 25 0.49302953626942525 2209 1 0.1998880012851103 2209 2 -1.4783054065995493 2209 3 -0.39889645572065646 2209 4 0.0852852862642095 2209 5 0.12685793523730382 2209 6 0.14191050828810603 2209 7 0.2656670660029903 2209 8 0.35282310443098197 2209 9 -2.126292767502406 2209 10 -0.38782110932858416 2209 11 0.01062489138305267 2209 12 0.27844660148320344 2209 13 0.37322988149886527 2209 14 0.6238253276286372 2209 15 0.7597799689965541 2209 16 0.6851422270572556 2209 17 0.4406454410965708 2209 18 0.7274233711562346 2209 19 0.4561743500862505 2209 20 0.3806321405915156 2209 21 0.37287536223646356 2209 22 0.3935093674556711 2209 23 0.3465678218958388 2209 24 0.4378997967769749 2209 25 0.5151526600268284 2210 1 0.18801096702687659 2210 2 0.28107649749964025 2210 3 0.23325299741025773 2210 4 0.36340331874902176 2210 5 0.18224897448641997 2210 6 0.17222903515385468 2210 7 0.19713858694579045 2210 8 0.3685791681667965 2210 9 0.5085708901413284 2210 10 -1.325854937550183 2210 11 -0.31011506063184413 2210 12 -2.1135482911448946 2210 13 -0.6464436874659754 2210 14 -0.23513868029460383 2210 15 -0.5431310990525589 2210 16 0.03771668301398279 2210 17 0.29705661538015704 2210 18 0.5656285747581009 2210 19 0.366094052248215 2210 20 0.3538740412530501 2210 21 0.3324797194935556 2210 22 0.3111430925070593 2210 23 0.2918755964530959 2210 24 0.26023740566730424 2210 25 0.27833420692655864 2211 1 -0.322742101926746 2211 2 -0.12209085874410874 2211 3 -1.5880971758448936 2211 4 -0.6715515421128231 2211 5 -0.4633187928771747 2211 6 -0.48530752001902044 2211 7 -0.3372834639959521 2211 8 -0.08679546216677636 2211 9 0.23958443713558353 2211 10 0.17508890466944393 2211 11 -0.1461848301911175 2211 12 -0.2865144910703066 2211 13 -0.30349939175384577 2211 14 -0.7042331660708712 2211 15 -0.37735358702800426 2211 16 -0.07816444066690309 2211 17 -0.05651542601898285 2211 18 0.15100072751678795 2211 19 0.004896639431713368 2211 20 0.034840750700929846 2211 21 -0.06068125458733675 2211 22 -0.07235490179539994 2211 23 -0.5367405247677226 2211 24 -0.20185504201909765 2211 25 0.0763345938834874 2212 1 -0.19140636121482757 2212 2 -0.20164799251002208 2212 3 -2.1259145792351504 2212 4 -1.2598930780994593 2212 5 -0.5540461992413483 2212 6 -0.37282356450133847 2212 7 -0.2285629315271578 2212 8 -0.2768258869738176 2212 9 -0.7152783700209113 2212 10 -0.04552022323493787 2212 11 -0.18935065116782113 2212 12 0.008943013587028759 2212 13 -0.32344328036622727 2212 14 0.04117999957290408 2212 15 0.15079109298376636 2212 16 -0.965207240334685 2212 17 -1.033948948316386 2212 18 -0.07488208497117199 2212 19 0.008590529970206262 2212 20 -0.030605713503504717 2212 21 0.018603720072782437 2212 22 -0.14145692698272683 2212 23 0.024836195208486345 2212 24 0.04831580378900213 2212 25 0.1441911974302954 2213 1 -3.142826589458636 2213 2 -1.366479368993076 2213 3 -2.487770183110957 2213 4 -1.1908946023972575 2213 5 -0.9008407027242029 2213 6 -0.7411227441762733 2213 7 -0.5125983532067195 2213 8 -0.27756136323208125 2213 9 -0.41615075556238423 2213 10 -0.3650395606433392 2213 11 -0.6618544464946083 2213 12 -0.34506595129862205 2213 13 -0.7252675040587278 2213 14 -0.3389426123519783 2213 15 -0.42312871641220084 2213 16 -1.7931434151180952 2213 17 -1.213438097844552 2213 18 -0.43184862956382375 2213 19 -0.27644822501871147 2213 20 -0.2296107571510433 2213 21 -0.11338330438073285 2213 22 -0.10677015836044745 2213 23 -0.037102098996406596 2213 24 0.0316704820881597 2213 25 0.037237398146295675 2214 1 -0.8893803111254037 2214 2 -0.7962809480959869 2214 3 -6.23397136573821 2214 4 -2.0757696837976845 2214 5 -1.3165527505105104 2214 6 -0.793797516451221 2214 7 -0.6526986228415481 2214 8 -0.4997598155124692 2214 9 -0.3033089718403801 2214 10 -0.28843659335258565 2214 11 -0.9271050290470697 2214 12 -0.596647538718102 2214 13 -5.30127235800871 2214 14 -4.0632706828807095 2214 15 -2.2754608876917715 2214 16 -3.1034128145725504 2214 17 -3.5906721309382377 2214 18 -1.3520707186976182 2214 19 -0.9127367316706625 2214 20 -0.42924304951784364 2214 21 -0.645908692160603 2214 22 -0.5205793322212955 2214 23 -0.4581880133880034 2214 24 -0.48241826464601445 2214 25 -0.17791079639876273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 898.0 3 122.0 4 109.0 5 279.0 6 40.0 7 42.0 8 74.0 9 240.0 10 622.0 11 1395.0 12 2954.0 13 5071.0 14 7892.0 15 11236.0 16 13934.0 17 15601.0 18 16771.0 19 17377.0 20 17397.0 21 17301.0 22 17676.0 23 18442.0 24 19341.0 25 21102.0 26 23337.0 27 26355.0 28 30895.0 29 37763.0 30 46659.0 31 58659.0 32 77286.0 33 103951.0 34 140007.0 35 194497.0 36 276309.0 37 417557.0 38 811273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95570603476835 21.659243694217924 13.17671222300698 22.208338048006745 2 14.702517536195197 21.95013106213407 41.44556226063808 21.901789141032655 3 17.87917094366771 28.355639067658682 30.078157855840367 23.68703213283324 4 11.473501599053755 17.9325927289905 39.758318937834716 30.83558673412103 5 12.161902300589263 39.491958818108955 35.34265915277204 13.003479728529733 6 29.197085657209453 39.80739562260551 18.15383848935053 12.841680230834504 7 24.586105289979468 34.27718695333313 24.188789235470807 16.947918521216597 8 23.266635606484094 38.115376464520956 21.889507143501717 16.728480785493236 9 24.180352804884176 17.35860629044436 22.78314778660854 35.677893118062926 10 14.168535210220847 31.260247043849077 34.223278670182296 20.34793907574778 11 30.96816949050115 27.1286556250342 23.515394232991458 18.387780651473193 12 19.65315936449708 29.355158653192436 32.442228955978734 18.549453026331747 13 25.58597434542617 25.47564350807963 27.449920334765483 21.488461811728715 14 19.449674574950663 24.482074161549168 29.29589036285332 26.772360900646845 15 22.293687823769666 31.585463757806608 24.91091867894218 21.209929739481545 16 20.44648282079928 32.54101252425472 26.75773211030752 20.254772544638485 17 18.695174946078403 31.453543308372733 29.527556161353996 20.323725584194868 18 18.974211856399794 30.88994922795764 30.626526348826488 19.50931256681607 19 19.78377208475154 29.74524308795515 30.84590238912449 19.62508243816882 20 21.54343698319688 29.16690826680822 29.80750934073787 19.48214540925703 21 21.841930325733482 29.716014236037953 28.890288101736385 19.55176733649218 22 20.123370230337105 29.539640220524493 30.47765741934404 19.859332129794367 23 20.077267849355053 29.961035312411692 29.98520958934764 19.976487248885615 24 20.061241233531412 29.070187697274896 30.20949680174233 20.659074267451363 25 20.585295097712528 29.197579564981606 29.36900934982457 20.848115987481297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2570.0 1 2570.0 2 2708.5 3 2847.0 4 2847.0 5 2847.0 6 4707.5 7 6568.0 8 6568.0 9 6568.0 10 10574.5 11 14581.0 12 14581.0 13 14581.0 14 23782.5 15 32984.0 16 32984.0 17 32984.0 18 49725.0 19 66466.0 20 66466.0 21 66466.0 22 89921.5 23 113377.0 24 113377.0 25 113377.0 26 143413.0 27 173449.0 28 173449.0 29 173449.0 30 202689.0 31 231929.0 32 231929.0 33 231929.0 34 253993.5 35 276058.0 36 276058.0 37 276058.0 38 286558.0 39 297058.0 40 297058.0 41 297058.0 42 289897.5 43 282737.0 44 282737.0 45 282737.0 46 280676.5 47 278616.0 48 278616.0 49 278616.0 50 256476.0 51 234336.0 52 234336.0 53 234336.0 54 205215.5 55 176095.0 56 176095.0 57 176095.0 58 149266.5 59 122438.0 60 122438.0 61 122438.0 62 99267.5 63 76097.0 64 76097.0 65 76097.0 66 56966.0 67 37835.0 68 37835.0 69 37835.0 70 26886.5 71 15938.0 72 15938.0 73 15938.0 74 10875.0 75 5812.0 76 5812.0 77 5812.0 78 3841.0 79 1870.0 80 1870.0 81 1870.0 82 1225.0 83 580.0 84 580.0 85 580.0 86 365.5 87 151.0 88 151.0 89 151.0 90 95.5 91 40.0 92 40.0 93 40.0 94 29.0 95 18.0 96 18.0 97 18.0 98 16.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09928731864659102 2 0.05076589576504695 3 0.053785732008305366 4 0.04909274325189026 5 0.046317758595923054 6 0.06570184258981156 7 0.06251877195502567 8 0.05937650991812163 9 0.0642735416639461 10 0.06476324483852854 11 0.05835629497107487 12 0.05888680674353918 13 0.0598254044948222 14 0.06174340859527012 15 0.06439596745759171 16 0.0594173185160035 17 0.06276362354231688 18 0.1407080454966896 19 0.06770146388602322 20 0.06178421719315199 21 0.05990702169058594 22 0.0642735416639461 23 0.06447758465335546 24 0.07292496441490265 25 0.06725256930932265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2450464.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.67884962168657 #Duplication Level Percentage of deduplicated Percentage of total 1 92.44161142582371 77.35407701287885 2 5.955149197518099 9.96640068347649 3 0.7919929869528188 1.9881918616996588 4 0.2709461861512775 0.90689860666089 5 0.13104048091015946 0.5482658348217362 6 0.08305309748287017 0.41698725929306213 7 0.054495237755879056 0.3192069163690582 8 0.036974328922286734 0.24751754478006463 9 0.029866218444660746 0.22492537217992872 >10 0.17747142574266728 2.7322449144200136 >50 0.014719400594569719 0.8541857077705751 >100 0.010571463052865281 1.6747481504234243 >500 0.0012259015396636804 0.6851658987026632 >1k 7.355409237783559E-4 1.3574474943350128 >5k 1.471081847556712E-4 0.7237367421885301 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6774 0.276437442051791 No Hit GTACATGGGAAGCAGTGGTATCAAC 5754 0.23481267221228305 No Hit GGTATCAACGCAGAGTACTTTTTTT 5110 0.20853193517635843 No Hit CCCATGTACTCTGCGTTGATACCAC 4881 0.19918676626141008 No Hit TATCAACGCAGAGTACTTTTTTTTT 3953 0.16131638742703422 No Hit ATTCCATTCCATTCCATTCCATTCC 2905 0.1185489768468339 No Hit GAGTACATGGGAAGCAGTGGTATCA 2833 0.11561075779933923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.080859788187054E-5 0.0 10 0.0 0.0 0.0 4.080859788187054E-5 0.0 11 0.0 0.0 0.0 8.161719576374108E-5 0.0 12 0.0 0.0 0.0 8.161719576374108E-5 0.0 13 0.0 0.0 0.0 8.161719576374108E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACCGT 65 5.468979E-5 13.150255 6 ATACCGT 80 2.8759618E-5 11.871758 6 CGAAGAA 60 0.0058624526 11.087099 14 AATACCG 60 0.005890147 11.080082 5 CGCCCTA 105 4.0878553E-5 9.956577 16 TGTCCCG 115 1.03049915E-5 9.910136 11 GACTGAC 110 6.857926E-5 9.497601 7 TAGACAG 400 0.0 9.497213 5 TATTCCG 120 1.7070255E-5 9.497213 5 GTCTTGC 435 0.0 9.391118 1 CTAGGAC 205 4.2564352E-10 9.265952 3 TTATACC 410 0.0 9.2655735 4 TCTACAC 300 0.0 9.181014 3 TCAGGAC 270 0.0 9.145838 3 GCGTGCA 85 0.007452737 8.938918 9 TCTATAC 395 0.0 8.896487 3 TAGGACT 290 0.0 8.842232 4 GTGTAAG 485 0.0 8.814727 1 GTCTAGA 250 2.5465852E-11 8.740292 1 CCGCCCT 120 1.7574015E-4 8.711471 15 >>END_MODULE