FastQCFastQC Report
Wed 25 May 2016
SRR1294662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450464
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167590.6839112919022683No Hit
GGTATCAACGCAGAGTACTTTTTTT140570.5736464604254542No Hit
TATCAACGCAGAGTACTTTTTTTTT108550.4429773300077046No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67150.2740297347767606No Hit
GTACATGGGAAGCAGTGGTATCAAC59930.2445659271060501No Hit
CCCATGTACTCTGCGTTGATACCAC55160.22510022591639786No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30860.12593533306345248No Hit
GAGTACATGGGAAGCAGTGGTATCA30810.12573129007404313No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29580.12071183253457304No Hit
ATTCCATTCCATTCCATTCCATTCC28560.11654935555062225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT350.002195506416.25800912
TGCTCGG600.005793192411.10481410
GAACCGT803.703342E-410.7088716
GTGTCGA750.002606298310.1529729
AGAACCG951.6118743E-410.0199975
TAGACAG3150.09.9722825
TATACTG8750.09.8997565
GTTATAC1752.57387E-99.7715553
GGTATCA49400.09.5675951
CTAGGAC2700.09.5001223
CGGTCAG1304.3356195E-69.48345116
CTATACT3750.09.39207654
CTTACAC4250.09.3883573
TAGGACA3500.09.24702554
CTAGTAC1856.699338E-99.2433623
TTAGACA3150.09.0657114
TAGGGTC1054.4049474E-49.065715
TTACCCT2106.4392225E-109.065714
GCCCTAG2002.6084308E-99.034351
TCTAGTA2356.548362E-118.8937322
Produced by FastQC (version 0.11.5)