##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2450464 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.488422192694934 31.0 31.0 34.0 29.0 34.0 2 31.63969966504303 31.0 31.0 34.0 29.0 34.0 3 31.699586282434673 31.0 31.0 34.0 29.0 34.0 4 35.29602556903509 37.0 35.0 37.0 32.0 37.0 5 35.474673367982554 37.0 35.0 37.0 33.0 37.0 6 35.12975012079345 37.0 35.0 37.0 32.0 37.0 7 34.992913586977814 37.0 35.0 37.0 32.0 37.0 8 35.04818964898077 37.0 35.0 37.0 32.0 37.0 9 36.49559511994463 39.0 37.0 39.0 32.0 39.0 10 36.27833381759536 39.0 37.0 39.0 32.0 39.0 11 36.45623155451376 39.0 37.0 39.0 32.0 39.0 12 36.394855423299425 39.0 37.0 39.0 32.0 39.0 13 36.276568437650994 39.0 37.0 39.0 32.0 39.0 14 37.400792666205255 40.0 37.0 41.0 31.0 41.0 15 37.46120571450958 40.0 37.0 41.0 32.0 41.0 16 37.50714476931716 40.0 37.0 41.0 32.0 41.0 17 37.564660815257845 40.0 37.0 41.0 32.0 41.0 18 37.57687605286183 40.0 37.0 41.0 32.0 41.0 19 37.617909098032044 40.0 37.0 41.0 32.0 41.0 20 37.6708480516343 40.0 37.0 41.0 32.0 41.0 21 37.51771950128629 40.0 37.0 41.0 31.0 41.0 22 37.51463641171631 40.0 37.0 41.0 31.0 41.0 23 37.529493189861185 40.0 37.0 41.0 31.0 41.0 24 37.46770734032412 40.0 37.0 41.0 31.0 41.0 25 36.506169035741806 39.0 35.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3759855568646593 1101 2 0.38784074011281433 1101 3 0.31121272999723715 1101 4 0.3089667393700566 1101 5 0.28067318359084936 1101 6 0.3113994173642922 1101 7 0.4433593808148899 1101 8 0.3220534974581639 1101 9 0.5154831938855793 1101 10 0.7212970826681016 1101 11 0.635793015778745 1101 12 0.5951901184627104 1101 13 0.5945525929865241 1101 14 0.8316223279957597 1101 15 0.8249079575002654 1101 16 0.7302941818355393 1101 17 0.7784951004700318 1101 18 0.7586561175014666 1101 19 0.8944410325612964 1101 20 0.8305016595340646 1101 21 1.025401173500775 1101 22 0.9783793147234618 1101 23 1.0097278775730487 1101 24 1.113109991631866 1101 25 1.0500465590974457 1102 1 0.33738547517596373 1102 2 0.34716780774931877 1102 3 0.2687691516847188 1102 4 0.21036153041211492 1102 5 0.23630448948025418 1102 6 0.3245039354444401 1102 7 0.35966279349054275 1102 8 0.3675382416595738 1102 9 0.5307398615081809 1102 10 0.5988400778623699 1102 11 0.5099322428646289 1102 12 0.5189363273250649 1102 13 0.5371432544042563 1102 14 0.7071352721780428 1102 15 0.6775778308827896 1102 16 0.7163701697038931 1102 17 0.7040430230569612 1102 18 0.6961099503524864 1102 19 0.721764815269367 1102 20 0.811581818740116 1102 21 1.0107919322497239 1102 22 0.9135854048764998 1102 23 0.8305989050256599 1102 24 0.9247210839140223 1102 25 0.8170992917581472 1103 1 0.2561438636230555 1103 2 0.19460081288556097 1103 3 0.14843546923014017 1103 4 0.13135389020582977 1103 5 0.18161320167371997 1103 6 0.21158130510487894 1103 7 0.19793960270734345 1103 8 0.23183258901671167 1103 9 0.04997077746590861 1103 10 0.26610562618503053 1103 11 0.3706506407449339 1103 12 0.3590129027924789 1103 13 0.5185350343330271 1103 14 0.5388951900536298 1103 15 0.6381713774575246 1103 16 0.6045868075275962 1103 17 0.5287510097256174 1103 18 0.4732325092493568 1103 19 0.5921421956808857 1103 20 0.4928025350398002 1103 21 0.8068289437256411 1103 22 0.7100555810208817 1103 23 0.6726198661726315 1103 24 0.8484761256525104 1103 25 0.7364795957710797 1104 1 0.13574928974903955 1104 2 0.1262015955738427 1104 3 0.08402186637997033 1104 4 0.11381862220539318 1104 5 0.059401032731173586 1104 6 0.06564795437576265 1104 7 0.12117957859223338 1104 8 0.08098298730843823 1104 9 -0.07724460685364676 1104 10 0.36766790526652926 1104 11 0.2774822314695058 1104 12 0.2906795069584689 1104 13 -0.1347629697318098 1104 14 0.2302924751808746 1104 15 0.4070223571969507 1104 16 0.4652902890356785 1104 17 0.4274110358332024 1104 18 0.4610034616439407 1104 19 0.5514175381896678 1104 20 0.41506362852553025 1104 21 0.6774917847128705 1104 22 0.6052335508985749 1104 23 0.5028260775101216 1104 24 0.679719601691275 1104 25 0.6466520289009381 1105 1 0.012723535054863788 1105 2 0.0847431458091954 1105 3 -0.11465437756253039 1105 4 -0.0772512144802846 1105 5 -0.001701901144961937 1105 6 -0.08187095792860788 1105 7 -0.009376585066604548 1105 8 -0.03818325573243442 1105 9 0.1495984986409269 1105 10 0.26161678354401374 1105 11 0.19113163876176031 1105 12 -0.11312866966840573 1105 13 -0.4382675215602774 1105 14 -0.6776317566706638 1105 15 -0.15344851471439114 1105 16 -0.027487768235722854 1105 17 0.09259233714849557 1105 18 -0.5881774320082656 1105 19 -0.6986904293783098 1105 20 -0.5688253913394519 1105 21 0.18151776463914615 1105 22 0.3130669531697663 1105 23 0.2828730306846552 1105 24 0.38138824001588745 1105 25 0.1881488505672806 1106 1 -0.42266815591248275 1106 2 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1 0.11664259895781726 2207 2 0.23435421344338536 2207 3 0.23333884105262825 2207 4 0.1383154437616767 2207 5 0.08739385838333646 2207 6 0.23304511976955666 2207 7 0.1813211890841515 2207 8 0.16502597551775722 2207 9 0.3057703099639397 2207 10 0.4486452407967292 2207 11 0.27767435559910325 2207 12 0.30894427614852304 2207 13 0.31701314819409276 2207 14 0.3311617891886911 2207 15 0.3932363107570609 2207 16 0.4192681851421298 2207 17 0.4278931703828661 2207 18 0.42783294459661647 2207 19 0.4830498723075465 2207 20 0.3393887780094218 2207 21 0.7416406385415257 2207 22 0.6321139305763168 2207 23 0.5087995458892252 2207 24 0.763393184204709 2207 25 0.5521971069058651 2208 1 0.18643782115344365 2208 2 0.15820362480117822 2208 3 0.18902004185539312 2208 4 0.12817315584405975 2208 5 0.09138995900422486 2208 6 0.27499467898331176 2208 7 0.21887293946539188 2208 8 0.10175397173091483 2208 9 0.23453376236090406 2208 10 0.2417738964646361 2208 11 -0.012226765925397842 2208 12 -0.24571732730184692 2208 13 0.08557346633714502 2208 14 -0.3068568312525599 2208 15 0.18535976206044324 2208 16 0.3043364417610803 2208 17 0.21736536338464418 2208 18 0.2986346189199409 2208 19 0.46106043076818537 2208 20 0.3564030619570815 2208 21 0.6890425333612882 2208 22 0.6881429473484815 2208 23 0.6332838700100893 2208 24 0.7289889760161401 2208 25 0.6727980264675821 2209 1 0.03707215757033211 2209 2 0.05559140227132886 2209 3 0.08535491300280995 2209 4 -0.4459746401724658 2209 5 -0.0024802074976832955 2209 6 0.09017195891399155 2209 7 0.1843232218313986 2209 8 0.2533021563079316 2209 9 0.26964779179851206 2209 10 0.2976139270116107 2209 11 0.3703470239379669 2209 12 0.360779271208159 2209 13 0.4032260671901895 2209 14 0.4990599869279606 2209 15 -0.17769486506642096 2209 16 -0.11745271723633977 2209 17 0.3187028001983734 2209 18 -0.09912928713417557 2209 19 0.32481090370389865 2209 20 0.4070154681112612 2209 21 0.6383795433039552 2209 22 0.5764538477514733 2209 23 0.5500784235667098 2209 24 0.6910942986796016 2209 25 0.7461146369845295 2210 1 -0.14818870741569867 2210 2 -0.10569553855942004 2210 3 0.001540521164216102 2210 4 0.03967136789729153 2210 5 -0.0825225957622564 2210 6 -0.387043192171312 2210 7 -0.1682249342445914 2210 8 -0.19739264960767144 2210 9 0.0317294111243811 2210 10 0.25112604677185146 2210 11 0.13473004517329912 2210 12 0.17617046468497932 2210 13 0.21545429414598516 2210 14 0.24686312501511765 2210 15 0.10579491805899721 2210 16 0.26684434641038735 2210 17 -0.6611211732387474 2210 18 -1.3156919211321707 2210 19 -0.20976688478864958 2210 20 0.0026121862270684915 2210 21 -0.13513594917247218 2210 22 -0.12939736838464455 2210 23 -0.0624460829097373 2210 24 0.3105168011903672 2210 25 0.26187262856653604 2211 1 -0.19024606633838204 2211 2 -0.16985719900734253 2211 3 -0.07238886882708329 2211 4 0.012334830934150887 2211 5 -0.016662395770310923 2211 6 0.02935130603660241 2211 7 -0.03998123717758517 2211 8 -0.02070908056912657 2211 9 -0.0715602480208446 2211 10 0.1190115547972681 2211 11 -0.14981299546768412 2211 12 -0.10461419115973314 2211 13 -0.05164780119950052 2211 14 -0.11054797557091689 2211 15 -0.1621723149188341 2211 16 -0.25171207663683504 2211 17 -0.2482026775731896 2211 18 -0.2814942089055563 2211 19 -0.15240375985759158 2211 20 -0.1005396304569075 2211 21 0.19475126426491585 2211 22 0.03588025132249584 2211 23 -0.647910291145088 2211 24 -0.030975136623375477 2211 25 -0.7265487360081693 2212 1 -0.1116741369787917 2212 2 -0.14203456430688277 2212 3 -0.10850782989966135 2212 4 0.019500531721696746 2212 5 0.011392720204526086 2212 6 0.016299032997650897 2212 7 -0.07838148094585762 2212 8 -0.2358746411359931 2212 9 0.027435556144787654 2212 10 -0.28621101087146883 2212 11 -0.011153420475039866 2212 12 -0.11987224344818515 2212 13 -0.1680621733575265 2212 14 -0.08673703928195664 2212 15 0.11405781342160992 2212 16 -0.15615437582463443 2212 17 -0.34200916069971043 2212 18 -0.2180998205065876 2212 19 -0.2691455704302257 2212 20 -0.3895674003162384 2212 21 0.06186808812770295 2212 22 -0.24253562474090984 2212 23 -0.20725965945860736 2212 24 -0.40149250472732945 2212 25 -0.14033632015983954 2213 1 -0.1961634047094165 2213 2 -0.16960426007431195 2213 3 -0.04157779444920351 2213 4 -0.06383076304748414 2213 5 -0.09997707156385616 2213 6 -0.26423466652347116 2213 7 -0.3516251722750354 2213 8 -0.2409328327205955 2213 9 -0.34177866474168894 2213 10 -0.3628070267291008 2213 11 -0.11683356256467903 2213 12 0.015673240344206363 2213 13 0.006072764514229334 2213 14 -0.5790025384509434 2213 15 -0.12122258850786238 2213 16 -0.27311802188741297 2213 17 -0.2671340698636868 2213 18 -0.3090702832537957 2213 19 -0.36205614667235864 2213 20 -0.3043566864453311 2213 21 0.014402297461174385 2213 22 -0.24152746054843988 2213 23 -0.35644031084665784 2213 24 -0.6284827000903022 2213 25 -0.35519174827373234 2214 1 -0.2695433073688882 2214 2 -0.25102351738858175 2214 3 -0.2976152870283606 2214 4 -0.15737129588356424 2214 5 -0.09303247092938705 2214 6 -0.17719884510489692 2214 7 -0.09514151381946334 2214 8 -0.15983943864314654 2214 9 -0.3002810537951959 2214 10 -0.3186800185249865 2214 11 -0.3388803289081821 2214 12 -0.4090442445983484 2214 13 -0.31055436791543656 2214 14 -0.396412422620422 2214 15 -0.19784857021662816 2214 16 -0.5117581836160099 2214 17 -0.7534302915762225 2214 18 -0.6009203024760623 2214 19 -0.6636862474388963 2214 20 -0.5953624949706082 2214 21 -0.724649246989344 2214 22 -1.0204551455856716 2214 23 -1.2322947864971425 2214 24 -0.6591321512748891 2214 25 -1.0301366313578768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 21.0 9 41.0 10 89.0 11 177.0 12 345.0 13 803.0 14 1643.0 15 2810.0 16 4188.0 17 5660.0 18 6912.0 19 7943.0 20 8663.0 21 9287.0 22 10187.0 23 11225.0 24 12457.0 25 14014.0 26 16426.0 27 18223.0 28 21651.0 29 27682.0 30 35879.0 31 47765.0 32 64954.0 33 90727.0 34 126779.0 35 182434.0 36 268681.0 37 442005.0 38 1010788.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79526968544221 22.040326497334537 12.471167203871305 23.69323661335195 2 14.6192721052013 22.384740196142445 40.225075740757674 22.770911957898583 3 17.816657062638697 27.90738447580398 30.504191034333378 23.771767427223946 4 11.504110914073836 17.84546881153872 39.65553621461236 30.994884059775078 5 12.207876070144847 38.90939311041829 35.60370986268022 13.279020956756638 6 29.50120855071056 39.74821656118549 17.68039078513756 13.070184102966397 7 24.514468886373535 34.480046272352965 24.01541649811845 16.990068343155052 8 23.582590948517783 37.981815398744835 21.422317465176793 17.01327618756059 9 24.14413958533466 16.406198351491845 23.064708719757697 36.3849533434158 10 14.243837927549277 30.916083231354207 34.18734961749122 20.652729223605295 11 31.439582799929926 26.266029155166592 23.384201132183183 18.9101869127203 12 19.94824583920135 28.908832772683663 32.62275085484961 18.52017053326537 13 26.225258747126052 24.531088423325887 27.71930354357668 21.524349285971383 14 19.74306602705234 23.789368010169625 29.76634760766966 26.70121835510838 15 22.578017589486954 30.79984378322106 25.390939834911347 21.23119879238064 16 20.451841879419323 31.924753689002756 27.231469696085508 20.391934735492413 17 18.488947399349676 31.131491831751045 29.941757969102994 20.437802799796284 18 18.768580717800802 30.202937656118838 31.363374260393062 19.665107365687298 19 19.566446979126283 29.036544308188294 32.04150258115857 19.355506131526862 20 21.200354709056548 28.415574388308478 31.06271110840867 19.321359794226307 21 21.572620302000686 29.071218523231167 29.999820441729668 19.356340733038476 22 19.844139477863987 28.878720010969655 31.54975767182121 19.72738283934515 23 19.721546385946816 29.268276757607342 31.226560542460973 19.783616313984872 24 19.63437113720958 28.564724299040627 31.410839502575637 20.390065061174152 25 20.26685993934111 28.611293392490445 30.499129946490893 20.622716721677556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1401.0 1 1401.0 2 1715.0 3 2029.0 4 2029.0 5 2029.0 6 4395.5 7 6762.0 8 6762.0 9 6762.0 10 10316.0 11 13870.0 12 13870.0 13 13870.0 14 21286.5 15 28703.0 16 28703.0 17 28703.0 18 45110.0 19 61517.0 20 61517.0 21 61517.0 22 87162.5 23 112808.0 24 112808.0 25 112808.0 26 144072.0 27 175336.0 28 175336.0 29 175336.0 30 205353.5 31 235371.0 32 235371.0 33 235371.0 34 257928.0 35 280485.0 36 280485.0 37 280485.0 38 287980.0 39 295475.0 40 295475.0 41 295475.0 42 288224.5 43 280974.0 44 280974.0 45 280974.0 46 280596.0 47 280218.0 48 280218.0 49 280218.0 50 258055.5 51 235893.0 52 235893.0 53 235893.0 54 207069.0 55 178245.0 56 178245.0 57 178245.0 58 150925.5 59 123606.0 60 123606.0 61 123606.0 62 99912.5 63 76219.0 64 76219.0 65 76219.0 66 57011.0 67 37803.0 68 37803.0 69 37803.0 70 26765.5 71 15728.0 72 15728.0 73 15728.0 74 10654.0 75 5580.0 76 5580.0 77 5580.0 78 3684.5 79 1789.0 80 1789.0 81 1789.0 82 1139.5 83 490.0 84 490.0 85 490.0 86 310.5 87 131.0 88 131.0 89 131.0 90 78.5 91 26.0 92 26.0 93 26.0 94 15.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09373734933465662 2 0.0 3 0.1458499288298053 4 0.01138559880904188 5 0.02293443200961124 6 0.017221228306149368 7 0.0253421392846416 8 0.024036264152421744 9 2.4485158729122317E-4 10 0.3919665826553665 11 0.06394707288089113 12 0.0013466837301017278 13 8.161719576374108E-5 14 0.0011834493385742455 15 9.385977512830223E-4 16 2.0404298940935266E-4 17 0.0 18 2.856601851730938E-4 19 5.713203703461876E-4 20 2.0404298940935266E-4 21 2.4485158729122317E-4 22 0.0027749846559671962 23 8.161719576374108E-5 24 4.080859788187054E-5 25 0.0016323439152748213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2450464.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.02795330792937 #Duplication Level Percentage of deduplicated Percentage of total 1 91.71936350653219 74.31832303640292 2 6.541879005178676 10.601501331554823 3 0.8783037179673335 2.1350145794891358 4 0.27510035128464416 0.8916327367554846 5 0.1396920500477562 0.5659480454379261 6 0.08550197638104089 0.4156830089963196 7 0.06382815014638422 0.36203050588549085 8 0.04651049450116911 0.3014920141415549 9 0.030553576516936336 0.2228124394264108 >10 0.18892631488670952 2.8973779719404424 >50 0.01606947008894374 0.8971673437244686 >100 0.011897679155642635 1.8112359258648494 >500 0.0012121058617207382 0.6712139693590773 >1k 8.585749853763049E-4 1.4575025443541396 >5k 1.515132327134656E-4 0.7457751114193014 >10k+ 1.515132327134656E-4 1.7052894352476793 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16759 0.6839112919022683 No Hit GGTATCAACGCAGAGTACTTTTTTT 14057 0.5736464604254542 No Hit TATCAACGCAGAGTACTTTTTTTTT 10855 0.4429773300077046 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6715 0.2740297347767606 No Hit GTACATGGGAAGCAGTGGTATCAAC 5993 0.2445659271060501 No Hit CCCATGTACTCTGCGTTGATACCAC 5516 0.22510022591639786 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3086 0.12593533306345248 No Hit GAGTACATGGGAAGCAGTGGTATCA 3081 0.12573129007404313 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2958 0.12071183253457304 No Hit ATTCCATTCCATTCCATTCCATTCC 2856 0.11654935555062225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.080859788187054E-5 0.0 0.0 0.0 0.0 9 4.080859788187054E-5 0.0 0.0 8.161719576374108E-5 0.0 10 4.080859788187054E-5 0.0 0.0 8.161719576374108E-5 0.0 11 4.080859788187054E-5 0.0 0.0 8.161719576374108E-5 0.0 12 4.080859788187054E-5 0.0 0.0 8.161719576374108E-5 0.0 13 4.080859788187054E-5 0.0 0.0 8.161719576374108E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGGAT 35 0.0021955064 16.258009 12 TGCTCGG 60 0.0057931924 11.104814 10 GAACCGT 80 3.703342E-4 10.708871 6 GTGTCGA 75 0.0026062983 10.152972 9 AGAACCG 95 1.6118743E-4 10.019997 5 TAGACAG 315 0.0 9.972282 5 TATACTG 875 0.0 9.899756 5 GTTATAC 175 2.57387E-9 9.771555 3 GGTATCA 4940 0.0 9.567595 1 CTAGGAC 270 0.0 9.500122 3 CGGTCAG 130 4.3356195E-6 9.483451 16 CTATACT 375 0.0 9.3920765 4 CTTACAC 425 0.0 9.388357 3 TAGGACA 350 0.0 9.2470255 4 CTAGTAC 185 6.699338E-9 9.243362 3 TTAGACA 315 0.0 9.065711 4 TAGGGTC 105 4.4049474E-4 9.06571 5 TTACCCT 210 6.4392225E-10 9.06571 4 GCCCTAG 200 2.6084308E-9 9.03435 1 TCTAGTA 235 6.548362E-11 8.893732 2 >>END_MODULE